Body fluid and hematological changes in a poikilothermic animal on cold exposure

1964 ◽  
Vol 207 (4) ◽  
pp. 865-867 ◽  
Author(s):  
Chandicharan Deb ◽  
Mohan Chand Boral

Body fluid and hematological values were compared in the toad ( Bufo melanostictus), a poikilothermic animal, kept at 6 C for 48 hr, and in the same animal at 32 C. Exposure to cold caused a reduction in total body water but an increase in plasma volume. There was no significant difference in blood volume and thiocyanate space between the two groups. The red blood cell count in the control animals of this amphibian class was found to be markedly low compared to mammals, and a further reduction occurred on exposure to cold. No notable alteration could be observed in the hemoglobin levels and hematocrit values due to either difference in class of vertebrates or to temperature exposure.

1981 ◽  
Vol 51 (4) ◽  
pp. 794-797 ◽  
Author(s):  
H. M. Biswas ◽  
P. B. Patra ◽  
M. C. Boral

Body fluid and hematologic changes were found in three groups of adult male toads that had been exposed to 48 h of continuous simulated altitudes of 12,000, 18,000, and 24,000 ft, respectively. Erythrocyte counts and hematocrit ratios were increased significantly in all the high-altitude-exposed animals compared with the control group of animals kept at sea level, whereas the hemoglobin concentrations were significantly increased only in the 18,000- and 24,000-ft-exposed animals. Exposure to high altitude generally caused a reduction of plasma volume, blood volume, extracellular fluid volume, and total body water. These reductions were markedly lower in the animals exposed to 24,000 ft. These simulated high-altitude effects on body fluids and hematology in the toad (Bufo melanostictus) were compared with those of the rat, birds, and humans acutely acutely exposed at various high altitudes and were found to be qualitatively similar.


1993 ◽  
Vol 73 (3_suppl) ◽  
pp. 1105-1106 ◽  
Author(s):  
Reuben T. Spitz ◽  
Hilliard G. Foster ◽  
Marc Hillbrand

Analysis of hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, and red blood-cell count for a group of 46 black and 59 white male forensic patients showed significant hematological differences between the groups, with white patients demonstrating higher mean values for all four measures.


2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Hüseyin Simavlı ◽  
Yasin Yücel Bucak ◽  
Mehmet Tosun ◽  
Mesut Erdurmuş

Purpose. The pathogenesis of pseudoexfoliation (PEX), the most common cause of secondary glaucoma, has not been clearly identified, but there is increasing evidence that points out the role of oxidative stress. The aim of this study is to evaluate some of the most commonly used blood parameters, hemoglobin (Hb), red blood cell count (RBC), alanine aminotransferase (ALT), and uric acid (UA) levels, in subjects with PEX.Materials and Methods. This study is performed in a state hospital between November 2011 and December 2012. Retrospective chart review of subjects who underwent cataract surgery was performed. Thirty-one healthy subjects with PEX and 34 healthy subjects without PEX were evaluated. Hb, RBC, ALT, and UA levels were recorded. Student’st-test was used to compare the two groups.Results. The mean age was73.6±14.1years in PEX group and70.1±12.7in control groupp=0.293. Hb, RBC, ALT, and UA levels did not show a statistically significant difference among PEX and control groups (p>0.05for all).Conclusion. Serum levels of Hb, RBC, ALT, and UA levels were similar in subjects with and without PEX. Further studies are needed to clarify the precise role of Hb, RBC, ALT, and UA in the pathogenesis of PEX.


Author(s):  
Benjamin Lebrun ◽  
Yves Jacquemyn

Abstract Background Fetal red blood cells (FRBC) in maternal blood are counted in rhesus-negative women to determine the amount of anti-D immunoglobulin to be administered in the case of a rhesus-positive fetus. In rhesus-positive pregnant women this is done in not always very well-defined indications including trauma, miscarriage, fetal death and diminished fetal movements. The aim of this study is to determine if the detection of FRBC is useful in rhesus-positive pregnant woman. This was done by assessing maternal and fetal characteristics that are more likely to give a positive test. Materials and methods This was a retrospective cohort study. Results A total of 169 FRBC tests were performed in 161 rhesus-positive pregnant women. FRBC were found in 45 (26.6%) of the women. Three patients experienced a miscarriage although their FRBC tests were negative (p = 0.295). Of the seven patients who experienced unexpected stillbirths, three tested positive. The deaths were not less likely to occur if the results had been negative (p = 0.631). There was a statistically significant difference between the different types of trauma indications (p = 0.025): the test was more likely positive if there had been a fall on the ground or staircase or blunt trauma (p = 0.041, 0.026 and 0.018, respectively). FRBC were not more frequently present in the absence of fetal movements (n = 16, p = 0.693). Conclusion FRBC in maternal blood were more likely positive in the case of a fall on the ground, or from a staircase. However, a positive test does not necessarily imply fetal pathology and, therefore, does not contribute to clinical management.


2012 ◽  
Vol 36 (0A) ◽  
pp. 50-55
Author(s):  
محمودسالم محمد شيت المعاضيدي

The aim of this experiment is to study the effect of antithyroid agent (propylthiouracil) (PTU) on some physiological parameters in domestic chicks. 36 local one 4 week of age day old chicks were used .chicks were distributed randomly in to three groups: (control group) fed on standard diet , while 2nd and 3rd groups were fed diet contain 0.01 and 0.1℅ of antithyroid agent propylthiouracil (PTU) respectively from 1-25 days of age . blood sample were taken from jugular vein at the end of the 4th week in order to study the effect of PTU treatment on blood picture .result revealed that PTU treatment in group treated with 0.1℅ PTU compared with control group significantly increased in red blood cell count ,Heterophils ,basophiles percentage and stress index at 4th weeks of age in group treated with 0.1℅ PTU compared with 0.01℅ PTU and control group significantly decreased in lymphocytes percentage, beside that no significant difference in total leukocytic count ,hemoglobin ,packed cell volume ,eosinophils , monocytes percentage and erythrocytes indexes in treated groups with compared group


The aim of this study was to identify erythrocyte changes resulting from heat stress in relation to different times of blood collection throughout the day. The experiment was carried out with 39 Rhode Island Red hens, in the initial laying phase, receiving standardized ad libitum feeding. The birds were placed in an environment of 20m2, with controlled temperature, divided into 2 distinct groups: Control Group with 12 animals at 16°C and Experimental Group with 27 animals at 30°C. Venipuncture took place at 7:00 am, 11:00 am and 3:00 pm. Blood was stored in an EDTA tube, aiming at cell preservation for further analysis. Total red blood cell count, hematimetry and erythroid morphometry were performed. After evaluating the erythroid and hematimetric parameters of the 39 birds, a negative correlation of heat stress was observed when comparing the different times of blood collection throughout the day. For morphometric correlation, there was a significant difference (P>0.01) at 3:00 pm. When evaluating the results between the times of blood collection from the same animal, it was observed that 55.5% reduced the hematocrit, while the total red blood cell count was reduced by 51.8%. It is essential that there is a systematization of time in the collection of blood from laying hens.


2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Moreno Zanardo ◽  
Fabio Martino Doniselli ◽  
Anastassia Esseridou ◽  
Massimiliano Agrò ◽  
Nicol Antonina Rita Panarisi ◽  
...  

Abstract Objectives Iodinated contrast media (ICM) could be more appropriately dosed on patient lean body weight (LBW) than on total body weight (TBW). Methods After Ethics Committee approval, trial registration NCT03384979, patients aged ≥ 18 years scheduled for multiphasic abdominal CT were randomised for ICM dose to LBW group (0.63 gI/kg of LBW) or TBW group (0.44 gI/kg of TBW). Abdominal 64-row CT was performed using 120 kVp, 100–200 mAs, rotation time 0.5 s, pitch 1, Iopamidol (370 mgI/mL), and flow rate 3 mL/s. Levene, Mann–Whitney U, and χ2 tests were used. The primary endpoint was liver contrast enhancement (LCE). Results Of 335 enrolled patients, 17 were screening failures; 44 dropped out after randomisation; 274 patients were analysed (133 LBW group, 141 TBW group). The median age of LBW group (66 years) was slightly lower than that of TBW group (70 years). Although the median ICM-injected volume was comparable between groups, its variability was larger in the former (interquartile range 27 mL versus 21 mL, p = 0.01). The same was for unenhanced liver density (IQR 10 versus 7 HU) (p = 0.02). Median LCE was 40 (35–46) HU in the LBW group and 40 (35–44) HU in the TBW group, without significant difference for median (p = 0.41) and variability (p = 0.23). Suboptimal LCE (< 40 HU) was found in 64/133 (48%) patients in the LBW group and 69/141 (49%) in the TBW group, but no examination needed repeating. Conclusions The calculation of the ICM volume to be administered for abdominal CT based on the LBW does not imply a more consistent LCE.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1806
Author(s):  
Ui-Hyung Kim ◽  
Seung-Hwan Lee ◽  
Sang-Rae Cho ◽  
Sung-Sik Kang ◽  
Shil Jin ◽  
...  

Hematological reference intervals must consider several parameters, including genotype, age, sex, management, and analytic process. Work is needed to evaluate hematological changes specifically in Hanwoo calves and according to calf sex. Therefore, in this study, we sought to confirm the complete blood cell count (CBC) reference intervals in Hanwoo calves, to monitor changes in hematologic values in Hanwoo calves from birth until 28 weeks of life, and to compare the hematologic values of male and female calves. A total of 35 male calves and 35 female calves was studied. Calf blood was sampled at multiple intervals from the time of birth until 28 weeks of age (including within 6 h of birth and at 2 days, 7 days, and 4 weeks and then at 4-week intervals through 28 weeks). In addition, blood samples were collected from 210 clinically healthy pregnant Hanwoo cows to establish CBC reference intervals for adult cattle. There were significant differences in the results of the cows and calves in all 14 parameters considered. The CBC reference intervals of the calves were wider than those of the cows in all parameters except mean corpuscular volume, mean corpuscular hemoglobin concentration, and mean platelet volume. We also identified differences from birth through 28 weeks between male and female calves at only some ages and some parameters. These results suggest that CBC reference intervals specific to Hanwoo calves are necessary for accurate diagnosis of calf diseases.


Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 414
Author(s):  
Gal Avishai ◽  
Idan Rabinovich ◽  
Hanna Gilat ◽  
Gavriel Chaushu ◽  
Liat Chaushu

Sialolithiasis is a chronic disease in which a sialolith (salivary stone) causes recurrent inflammation of the affected salivary gland. Anemia of inflammation is a well-described pathology in which a chronic inflammatory disease leads to a reduction in the red blood cell count, hemoglobin and hematocrit values. In this retrospective cohort study, we aim to find whether removal of the sialolith and alleviation of the inflammation affect the complete blood count results. We examined data regarding forty-nine patients who underwent surgery for the removal of a submandibular gland sialolith using the duct-stretching technique. Complete blood counts two years before and after the surgical procedure were collected. The average pre-procedure and post-procedure values were calculated for each patient to establish the average blood profile. The pre- and post-procedure values were compared to evaluate the effect of the surgical treatment on the blood profile. We found that the average blood count values for patients with sialolithiasis were towards the lower end of the normal range. Post-surgery, a significant increase in hematocrit, hemoglobin and red blood cell count was observed, which was more pronounced in the older age group and in patients with co-morbidities. We conclude that sialolith removal surgery is associated with significant improvement in the complete blood count values, especially in the elderly and in patients and with co-morbidities. The speculated pathogenesis is relative anemia of inflammation.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alejandro Gil-Betacur ◽  
Carmen Yulieth Mantilla-Gutiérrez ◽  
Jaiberth Antonio Cardona-Arias

AbstractThe effects of platelet donation by apheresis on different parameters of the erythrogram are still unclear. The objective was to meta-analyze the effect of plateletpheresis on hematocrit, hemoglobin, and erythrocyte count, with a systematic review with random effects meta-analysis of the mean difference. The PRISMA guidelines were considered, as well as 133 search strategies on four different databases. Reproducibility was guaranteed and methodological quality was evaluated. Heterogeneity was evaluated with Galbraith and DerSimonian-Laird’s, publication bias with a funnel plot and a Begg’s test, sensitivity analysis and a cumulative meta-analysis were also conducted. Eighteen (18) articles were included, 17 evaluated the effects on hematocrit in 2,564 donors; 13 on hemoglobin in 1,640 donors; and 4 on red blood cell count in 243 donors. A decrease of 2.26% (CI95% = 2.11–2.41) was observed in hematocrit, of 0.80 g/dL (CI95% = 0.75–0.86) in hemoglobin and −0.21 × 1012/L (CI95% = −0.13; −0.29) in red blood cell count. Plateletpheresis has a negative effect on the erythrogram parameters, explained by blood loss in the kits used for the procedure and cell lysis. Such evidence is relevant to secure the efficiency and safety of the procedure, improve selection processes or determine the number of donations that can be performed without affecting donors’ health.


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