Extracellular space of swine aorta measured with [14C]inulin and [14C]sucrose

1976 ◽  
Vol 231 (6) ◽  
pp. 1806-1810 ◽  
Author(s):  
RG Harrison ◽  
TA Massaro
Keyword(s):  
Thoracic Aorta ◽  
Incubation Medium ◽  
Extracellular Space ◽  
Cell Damage ◽  
Significant Alteration ◽  
Ion Content ◽  
Tissue Water ◽  
Significant Difference ◽  
Ion Contents ◽  
Transient Measurements

Measurements of the extracellular space (ECS) of the isolated swine thoracic aorta were performed with both [14C]inulin and transient measurements and appeared to have better access to available tissue water than the [14C]sucrose gave more consistent results in available tissue water than the [14C]inulin. With [14C]sucrose as the tracer, no significant difference in the ECS was found when the tissue was incubated for 1.5 h in the presence of oxygen and glucose as compared to an incubation in which both oxygen and glucose were absent. However, the ion contents were markedly altered by this change in incubation medium. When oxygen and glucose were present tissue K+ was significantly higher and tissue Na+ was significantly lower than when these metabolites were deleted from the medium. Thus, significant alteration in ion content did not lead to substantial cell damage or bursting.

Osmotic and ionic effects of NaCl on germination, early seedling growth, and ion content of Atriplex halimus (Chenopodiaceae)

2002 ◽  
Vol 80 (3) ◽  
pp. 297-304 ◽  
Author(s):  
Mohammed Bajji ◽  
Jean-Marie Kinet ◽  
Stanley Lutts
Keyword(s):  
Calcium Concentration ◽  
Deionized Water ◽  
Ion Content ◽  
Short Term ◽  
Atriplex Halimus ◽  
Early Seedling Growth ◽  
Significant Difference ◽  
Early Seedling ◽  
Ionic Effects ◽  
Higher Doses

The effects of salt and osmotic stresses on the germination processes in seeds of the perennial halophyte species Atriplex halimus L. were compared using iso-osmotic concentrations of NaCl and mannitol. The lowest stress intensity delayed germination, while higher doses of NaCl and mannitol reduced final germination percentages. No significant difference occurred between the effects of these solutes on germination percentages or seedling dry weights. At an external osmotic potential of –0.7 MPa, however, the water content of mannitol-treated seedlings was reduced compared to that of seedlings that developed from NaCl-exposed seeds. The K, Mg, and Pi content decreased in seedlings that developed from mannitol-treated seeds while calcium concentration was strongly reduced in those arising from NaCl-treated seeds. Inhibited seeds were able to germinate at levels similar to those of the control after rinsing in deionized water and imbibition in control conditions. Seedlings produced from NaCl pre-treated seeds had a lower Ca and a higher Na content than control seedlings. The effect of salinity on the germination phase of development is mainly due to its osmotic component, and inhibition of germination is reversible. Both salt and osmotic stresses may have an impact on the mobilization of minerals from the seeds to the young seedling, but this effect does not have any consequence on growth processes analysed on a short-term basis.Key words: Atriplex halimus, halophyte, osmotic stress, recovery of seed germination, salinity.

Abstract 1683: Ca2+/Redox-Sensitive Tyrosine Kinase PYK2 Promotes Atherogenesis through OxLDL/ROS/p21-Dependent Premature Senescence and Cytokine Induction in Endothelium

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Asako Katsume ◽  
Mitsuhiko Okigaki ◽  
Akihiro Matsui ◽  
Shinichiro Yamaguchi ◽  
Eigo Kishita ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Tyrosine Kinase ◽  
Thoracic Aorta ◽  
Oxidized Ldl ◽  
Small Gtpase ◽  
Tnf Alpha ◽  
Premature Senescence ◽  
Ros Production ◽  
Cytokine Induction ◽  
Significant Difference

Background: Calcium/redox-sensitive tyrosine kinase PYK2 plays crucial roles in cell migration. Analysis of PYK2-deficient macrophages has been reported that their directional migration was impaired due to dysfunction of PI3K, small GTPase Rho, and Ca (2+)-mobilization. Since monocyte/macrophage plays crucial role in atherogenesis, we newly generated the PYK2-knock-out mice and investigated the role of PYK2 in atherogenesis. Methods: PYK2−/− mice (P-KO) were crossbred with ApoE−/− mice (E-KO), and PYK2−/−/ApoE−/− double knockout (D-KO) mice were established. Four-week-old mice were fed with high-fat-diet for 8 weeks, and the thoracic aorta and aortic root were histologically analyzed. Then we studied the mechanisms of the difference in atherogenesis. Results: At the 8th week, atherosclerotic area of thoracic aorta in D-KO was less than E-KO (~54%, P < 0.01, n = 15, respectively). Numbers of infiltrating macrophage in D-KO mice was also reduced (~52%, P < 0.01, n = 15). We also verified that no significant difference in atherosclerotic area between E-KO recipient with D-KO bone marrow and D-KO recipient with E-KO bone marrow. At the 7th day, the expression of VCAM1, and MCP-1 in the thoracic aorta in D-KO mice was inhibited (~43 to 58%, n = 5, P < 0.01, respectively). Inflammatory cells were barely detected in the region at that time. TNF-alpha and MCP-1 mRNA of peripheral blood-leukocytes in D-KO was also 38% lower (P < 0.05, n = 5 each). In the primary-cultured P-KO endothelial cells, TNF-alpha-induced expressions of VCAM-1 and MCP-1 was decreased to 31 and 32% respectively, compared to the wild-type (WT) (each n = 5, P < 0.01). Lysophosphatidylcholine (LPC), the component of Oxidized LDL, -induced Reactive Oxygen Species (ROS)-production was attenuated to 54% of the WT (p < 0.05) using DCF staining, and the high cholesterol diet induced ROS-production in endothelium in D-KO were decreased by 8-OHDG staining. Senescence-associated-beta-gal-stained area and ROS-dependent expression of p21 in the thoracic aorta in D-KO-mice was diminished. In P-KO ECs, LPC-induced p21 expression via Ets-1 or STAT1 was also decreased markedly. Conclusion: PYK2 promotes atherogenesis by inducing ROS/p21-dependent premature senescence and cytokine induction in endothelium.

Espace extracellulaire, perméabilité à l'inuline et accumulation de L-alanine dans l'intestin isolé de Grenouille

1973 ◽  
Vol 51 (1) ◽  
pp. 22-28
Author(s):  
Joël de la Noüe ◽  
André Gagnon
Keyword(s):  
Extracellular Space ◽  
Muscle Layer ◽  
Solute Concentration ◽  
Tissue Water ◽  
Intracellular Amino Acid ◽  
Inulin Space ◽  
Total Tissue ◽  
Frog Intestine

In order to calculate the intracellular concentration of accumulated L-alanine, the extracellular space (inulin-14C) of frog intestine was measured. To check the validity of the technique, frog liver and gastrocnemius were used too. By scraping proximal portions of intestine, the inulin space was found to be similar (around 20% of total tissue water) in both the muscle layer and the mucosa. The mucosal epithelium is an imperfect barrier to inulin while the serosa is very permeable. These results suggest that the interstitial solute concentration is best approximated by equating it to that of the serosal solution. The in vitro inulin space, compared to the in vivo one, increases with time, as does the cellular hydration. The data obtained from measurements of extracellular space and from L-alanine uptake show that the intracellular amino acid is in a free state.

O2-. release by activated Kupffer cells upon hypoxia-reoxygenation

1991 ◽  
Vol 261 (4) ◽  
pp. G602-G607 ◽  
Author(s):  
B. Rymsa ◽  
J. F. Wang ◽  
H. de Groot
Keyword(s):  
Nadph Oxidase ◽  
Kupffer Cells ◽  
Cell Injury ◽  
Cell Damage ◽  
Primary Cultures ◽  
Time Lag ◽  
Specific Inhibitor ◽  
Significant Difference ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

Primary cultures of rat liver Kupffer cells generated large amounts of superoxide anion radical (O2-.) when subjected to reoxygenation after a hypoxic period of at least 2 h. O2-. formation reached its maximum rate of approximately 25 nmol/10(6) cells within 1 h after reoxygenation. Two to four hours after reoxygenation, the number of injured cells began to increase and after 10 h approximately 60% of the cells were dead. During the period of O2-. release no significant difference in cell viability was observed between reoxygenated and hypoxically incubated cells, indicating a distinct time lag between O2-. release and onset of cell damage. Addition of diphenyliodonium, a specific inhibitor of the neutrophilic NADPH oxidase, to the Kupffer cells just before reoxygenation diminished both O2-. formation and cell injury up to 70%. Reoxygenation injury was completely prevented when superoxide dismutase and catalase were added immediately before reoxygenation. The results indicate that Kupffer cells subjected to hypoxia-reoxygenation generate a burst of reactive oxygen species and that this kind of "activation," probably by activating the NADPH oxidase, contributes to the self-destruction of the cells.

scholarly journals The The Role Of Von Willenbrand Factors As The Early Detection Of Endotel Cell Damage In Youth Ages With Obesity In The Usu Medan Campus Environment

2021 ◽  
Vol 6 (1) ◽  
pp. 179-181
Author(s):  
Rusdiana ◽  
Muhammad Syahputra ◽  
Sry Suryani
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Vascular System ◽  
Cell Damage ◽  
Single Layer ◽  
Control Group ◽  
Research Subjects ◽  
Cross Sectional ◽  
Significant Difference ◽  
Obese Subjects

Preliminary : Endothelial cells are a single layer that lines the entire vascular system. Endothelial dysfunction can be triggered by several main things, namely physical stress, oxidative stress and irritant substances. Obesity triggers an inflammatory process and metabolic disorders that will lead to increased oxidative stress. Long-term oxidative stress will cause damage to cells and tissues and trigger degenerative diseases. Damage to endothelial cells is expected to be detected by examining Von Willenbrand levels so that it can prevent complications of vascular disorders early. Method: This research is descriptive with cross sectional design. Carried out from March to October 2018 on the USU Campus. The first examination was done to measure body weight and height to determine body mass index, then performed lipid profile and blood sugar levels (KGD) in the sample, then examined von Willenbrand factor levels carried out in the integrated laboratory of USU FK using the method ELISA in both the sample group and the control group. The research subjects were adolescents aged 17-25 years with BMI> 25 kg / m2Data analysis was carried out using the T-Test statistical program, comparing two groups. Result: Of the 40 obese subjects found Von Wilenbrand level values ​​The lowest factor was 1.78 IU / ml and the highest was 35.60 IU / ml. Whereas in 40 non-obese subjects Von Wilenbrand grade values ​​were the lowest factor of 2.01 IU / ml and the highest was 45.10 IU / ml. This difference was not statistically significant (p = 0.661).Conclusion: There was no significant difference between the levels of Von Wilenbrand Factors in obese subjects with non-obese subjectsKey Words: Obesity, endothelial cells, Von Wilenbrand Factors

scholarly journals THE EFFECTS OF PANORAMIC RADIOGRAPHY ON GINGIVAL CREVICULAR FLUID VOLUME IN GINGIVITIS

2021 ◽  
Vol 6 (2) ◽  
pp. 136
Author(s):  
Rini Rahmiyati ◽  
Didit Aspriyanto ◽  
Beta Widya Oktiani
Keyword(s):  
Filter Paper ◽  
Wistar Rats ◽  
Gingival Crevicular Fluid ◽  
Panoramic Radiography ◽  
Cell Damage ◽  
Biological Fluid ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Crevicular Fluid

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography

Abstract 13051: Nourin-dependent Mirna-137 : A Novel Early Diagnostic Biomarker for Unstable Angina Patients

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Salwa A Elgebaly ◽  
Robert H Christenson ◽  
Hossam Kandil ◽  
Nashwa El-Khazragy ◽  
Laila Rashed ◽  
...  
Keyword(s):  
Gene Expression ◽  
Unstable Angina ◽  
Cell Damage ◽  
Invasive Coronary Angiography ◽  
Diagnostic Biomarker ◽  
Significant Difference ◽  
Early Biomarker ◽  
Artery Disease ◽  
Spearman’S Correlation ◽  
Early Diagnostic

Introduction: No blood biomarker exits that can diagnose Unstable Angina (UA) patients. Nourin is ischemia-dependent inflammatory mediator rapidly released by reversible ischemic myocardium “before” necrosis, and by necrotic cells. Using Nourin amino acid sequence, Bioinformatics analysis indicated that Nourin is likely regulated by miR-137; a marker of cell damage and a hypoxiaresponsive autophagy-signaling pathway linked to myocardial ischemia and coronary artery disease. Hypothesis: That the Nourin-dependent miR-137, is an early biomarker for UA patients when Troponin levels are below the decision limit. The underlying regulatory mechanism involves lncRCTB89H12.4 and mRNA-FTLH-17; also associated with ischemia. Methods: We measured serum gene expression profile of lnc-CTB89H12.4/miR-137 and mRNAFTHL- 17 in UA (n=30 - confirmed by invasive coronary angiography and negative Troponin) and STEMI (n=16) patients at presentation, and healthy volunteers (n=16). Results: Gene expression of miR-137 was up-regulated by 1,185-fold in UA (median=1,244.41) compared to healthy (1.05), and by 2.5-fold in STEMI (3,162.72) compared to UA (Fig. 1). Receiving Operator Characteristics (ROC) analysis revealed a statistically significant difference in miR-137 that discriminated UA from healthy controls with a test sensitivity & specificity of 96% & 93%, respectively. Diagnostic sensitivity was 75% & specificity was 83% for discriminating UA from STEMI. Additionally, Spearman’s correlation analysis revealed a significant association of miR-137 with lncRCTB89H12.4and mRNA-FTHL-17. The down-regulation of lncR-CTB89H12.4 after ischemia resulted in the up-regulation of miR-137 and activation of mRNA-FTLH-17. Conclusions: As a marker of cell damage, the Nourin-dependent miR-137 is a promising early diagnostic biomarker indicating UA patients and discriminating between UA and STEMI. Regulations appears to be from lncR-CTB89H12.4 and mRNA-FTLH-17.

Secretion of tetraethylammonium by proximal tubules of rabbit kidneys

1983 ◽  
Vol 245 (2) ◽  
pp. F238-F246 ◽  
Author(s):  
C. Schali ◽  
L. Schild ◽  
J. Overney ◽  
F. Roch-Ramel
Keyword(s):  
Membrane Permeability ◽  
Cellular Uptake ◽  
Incubation Medium ◽  
Proximal Tubules ◽  
Tissue Water ◽  
Secretory Rate ◽  
Luminal Membrane ◽  
Secretory Transport ◽  
Energy Dependent

The secretory transport of tetraethylammonium (TEA) was investigated in perfused and nonperfused isolated S1, S2, and S3 segments of proximal tubules from rabbit kidneys. In the perfused tubules the transepithelial net secretory flux and in nonperfused tubules the TEA cellular uptake were saturable (Km = 67 microM, Vmax = 2,480 fmol X min-1 X mm-1 in perfused S2 segments), energy dependent, and inhibited by mepiperphenidol. The net secretory flux of TEA (J b leads to j TEA) at a bath TEA concentration of 40 microM differed for the three segments and decreased in the order S1 greater than S2 greater than S3. The concentration of TEA in the perfusate leaving the tubule was approximately twice as great and the intracellular TEA concentration approximately 40 times as great as that in the bath. In nonperfused segments (40 microM TEA in the incubation medium) the TEA tissue water-to-medium ratio reached 100. In the three segments the ability to accumulate TEA across the peritubular membrane, thus, was similar, but the transepithelial secretory flux differed significantly. The differences in secretory rate between the three segments presumably result from differences in the luminal membrane permeability.

Osmotic balance and respiration in the hermit crab, Pagurus bernhardus, exposed to fluctuating salinities

1978 ◽  
Vol 58 (4) ◽  
pp. 869-876 ◽  
Author(s):  
Sandra E. Shumway
Keyword(s):  
Oxygen Consumption ◽  
Sea Water ◽  
External Medium ◽  
Temporary Increase ◽  
Tissue Water ◽  
Low Salinity ◽  
Significant Difference ◽  
Rate Of Oxygen Consumption ◽  
Osmotic Balance ◽  
Pagurus Bernhardus

Specimens of Pagurus bernhardus (with and without shells) were exposed to both gradual (sinusoidal) and abrupt (square-wave) salinity fluctuations and changes in haemolymph osmolality, tissue water content and oxygen consumption monitored. Oxygen consumption was also monitored under steady-state conditions; under these conditions there was no significant difference between the rate of oxygen consumption by animals with shells and animals without shells. Oxygen consumption was found to vary with body weight according to the equation O2 consumption = 0·292 W0·668. During exposure to fluctuating salinities the crabs with shells were seen to increase loco-motory activity when the external medium declined to approximately 75% sea water. Haemolymph osmolality values followed the same pattern of change as the external medium; the haemolymph of crabs without shells became significantly more dilute during exposure to low salinity than did that of crabs with shells. P. bernhardus showed significant increases and decreases in hydration level as salinities fell and rose respectively. Crabs with shells showed a marked temporary increase in oxygen consumption when the external medium declined to approximately 75% sea water; crabs without shells showed no such response. The importance of the shell as a means of protection against dilute media is discussed.

Chemical and histological space determinations in rabbit heart

1962 ◽  
Vol 202 (3) ◽  
pp. 589-592 ◽  
Author(s):  
John A. Johnson ◽  
Mary A. Simonds
Keyword(s):  
Weight Change ◽  
Extracellular Space ◽  
Chemical Space ◽  
Freeze Substitution ◽  
Rabbit Heart ◽  
Quantitative Agreement ◽  
Tissue Water ◽  
Histological Techniques ◽  
Chemical Technique ◽  
Osmotic Activity

The extracellular space of perfused rabbit heart ventricles was estimated by chemical and histological techniques. Sucrose and thiocyanate were used for the chemical space estimations. The method proposed by Chalkley following fixation by freeze substitution was used for the histological extracellular space determination. The extracellular space fraction was altered by using perfusing solutions of different osmotic activity to shrink or swell the cells. The magnitude of the tissue water loss or gain was determined by weighing the heart. Using the assumption that the cells alone changed in volume during an osmotically induced weight change, an alteration in the fractional extracellular space was predicted from the original chemical or histological space determination and the magnitude of the weight change. This predicted change when compared to the observed change in the histological and chemical spaces always agreed in direction with the measured values of both the histological and chemical spaces but was in better quantitative agreement with the values from the chemical technique.

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