Oral tolerance attenuates changes in in vitro lung tissue mechanics and extracellular matrix remodeling induced by chronic allergic inflammation in guinea pigs

Recent studies emphasize the presence of alveolar tissue inflammation in asthma. Immunotherapy has been considered a possible therapeutic strategy for asthma, and its effect on lung tissue had not been previously investigated. Measurements of lung tissue resistance and elastance were obtained before and after both ovalbumin and acetylcholine challenges. Using morphometry, we assessed eosinophil and smooth muscle cell density, as well as collagen and elastic fiber content, in lung tissue from guinea pigs with chronic pulmonary allergic inflammation. Animals received seven inhalations of ovalbumin (1–5 mg/ml; OVA group) or saline (SAL group) during 4 wk. Oral tolerance (OT) was induced by offering ad libitum ovalbumin 2% in sterile drinking water starting with the 1st inhalation (OT1 group) or after the 4th (OT2 group). The ovalbumin-exposed animals presented an increase in baseline and in postchallenge resistance and elastance related to baseline, eosinophil density, and collagen and elastic fiber content in lung tissue compared with controls. Baseline and post-ovalbumin and acetylcholine elastance and resistance, eosinophil density, and collagen and elastic fiber content were attenuated in OT1 and OT2 groups compared with the OVA group. Our results show that inducing oral tolerance attenuates lung tissue mechanics, as well as eosinophilic inflammation and extracellular matrix remodeling induced by chronic inflammation.

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Lung Tissue Mechanics and Extracellular Matrix Remodeling in Acute Lung Injury

American Journal of Respiratory and Critical Care Medicine ◽

10.1164/ajrccm.164.6.2007062 ◽

2001 ◽

Vol 164 (6) ◽

pp. 1067-1071 ◽

Cited By ~ 112

Author(s):

PATRICIA R. M. ROCCO ◽

ELNARA M. NEGRI ◽

PEDRO M. KURTZ ◽

FERNANDA P. VASCONCELLOS ◽

GABRIELA H. SILVA ◽

...

Keyword(s):

Extracellular Matrix ◽

Acute Lung Injury ◽

Lung Injury ◽

Lung Tissue ◽

Tissue Mechanics ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling

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Effects Of Rho Kinase Inhibition On Airway And Lung Tissue Inflammation, Extracellular Matrix Remodeling And Oxidative Stress Activation In Animals With Chronic Pulmonary Inflammation

10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a5639 ◽

2012 ◽

Author(s):

Patricia A.D.S. Pigati ◽

Samantha S. Possa ◽

Renato F. Righetti ◽

Fabio C. Habrum ◽

Homar T. Charaffeddine ◽

...

Keyword(s):

Oxidative Stress ◽

Extracellular Matrix ◽

Lung Tissue ◽

Pulmonary Inflammation ◽

Rho Kinase ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling ◽

Tissue Inflammation ◽

And Oxidative Stress ◽

Stress Activation

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Effects of chronic l-NAME treatment lung tissue mechanics, eosinophilic and extracellular matrix responses induced by chronic pulmonary inflammation

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.00199.2007 ◽

2008 ◽

Vol 294 (6) ◽

pp. L1197-L1205 ◽

Cited By ~ 29

Author(s):

Patrícia Angeli ◽

Carla M. Prado ◽

Débora G. Xisto ◽

Pedro L. Silva ◽

Caroline P. Pássaro ◽

...

Keyword(s):

Nitric Oxide ◽

Extracellular Matrix ◽

Smooth Muscle ◽

Lung Tissue ◽

Pulmonary Inflammation ◽

Elastic Fiber ◽

Tissue Mechanics ◽

Elastic Fibers ◽

Distal Lung ◽

Alveolar Septa

The importance of lung tissue in asthma pathophysiology has been recently recognized. Although nitric oxide mediates smooth muscle tonus control in airways, its effects on lung tissue responsiveness have not been investigated previously. We hypothesized that chronic nitric oxide synthase (NOS) inhibition by Nω-nitro-l-arginine methyl ester (l-NAME) may modulate lung tissue mechanics and eosinophil and extracellular matrix remodeling in guinea pigs with chronic pulmonary inflammation. Animals were submitted to seven saline or ovalbumin exposures with increasing doses (1∼5 mg/ml for 4 wk) and treated or not with l-NAME in drinking water. After the seventh inhalation (72 h), animals were anesthetized and exsanguinated, and oscillatory mechanics of lung tissue strips were performed in baseline condition and after ovalbumin challenge (0.1%). Using morphometry, we assessed the density of eosinophils, neuronal NOS (nNOS)- and inducible NOS (iNOS)-positive distal lung cells, smooth muscle cells, as well as collagen and elastic fibers in lung tissue. Ovalbumin-exposed animals had an increase in baseline and maximal tissue resistance and elastance, eosinophil density, nNOS- and iNOS-positive cells, the amount of collagen and elastic fibers, and isoprostane-8-PGF2α expression in the alveolar septa compared with controls ( P < 0.05). l-NAME treatment in ovalbumin-exposed animals attenuated lung tissue mechanical responses ( P < 0.01), nNOS- and iNOS-positive cells, elastic fiber content ( P < 0.001), and isoprostane-8-PGF2α in the alveolar septa ( P < 0.001). However, this treatment did not affect the total number of eosinophils and collagen deposition. These data suggest that NO contributes to distal lung parenchyma constriction and to elastic fiber deposition in this model. One possibility may be related to the effects of NO activating the oxidative stress pathway.

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Lung tissue mechanics and extracellular matrix composition in a murine model of silicosis

Journal of Applied Physiology ◽

10.1152/jappl.2001.90.4.1400 ◽

2001 ◽

Vol 90 (4) ◽

pp. 1400-1406 ◽

Cited By ~ 47

Author(s):

Débora S. Faffe ◽

Gabriela H. Silva ◽

Pedro M. P. Kurtz ◽

Elnara M. Negri ◽

Vera L. Capelozzi ◽

...

Keyword(s):

Mechanical Properties ◽

Extracellular Matrix ◽

Lung Tissue ◽

Elastic System ◽

Elastic Fiber ◽

Dynamic Mechanical Properties ◽

Tissue Mechanics ◽

Matrix Composition ◽

Elastic Fibers ◽

Oxytalan Fibers

The dynamic mechanical properties of lung tissue and its contents of collagen and elastic fibers were studied in strips prepared from mice instilled intratracheally with saline (C) or silica [15 (S15) and 30 days (S30) after instillation]. Resistance, elastance, and hysteresivity were studied during oscillations at different frequencies on S15 and S30. Elastance increased from C to silica groups but was similar between S15 and S30. Resistance was augmented from C to S15 and S30 and was greater in S30 than in S15 at higher frequencies. Hysteresivity was higher in S30 than in C and S15. Silica groups presented a greater amount of collagen than did C. Elastic fiber content increased progressively along time. This increment was related to the higher amount of oxytalan fibers at 15 and 30 days, whereas elaunin and fully developed elastic fibers were augmented only at 30 days. Silicosis led not only to pulmonary fibrosis but also to fibroelastosis, thus assigning a major role to the elastic system in the silicotic lung.

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Faculty Opinions recommendation of Sweet, yet underappreciated: Proteoglycans and extracellular matrix remodeling in heart disease.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732498270.793541733 ◽

2018 ◽

Author(s):

Nikos Karamanos

Keyword(s):

Extracellular Matrix ◽

Heart Disease ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling

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The Dynamic Interaction between Extracellular Matrix Remodeling and Breast Tumor Progression

Cells ◽

10.3390/cells10051046 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1046

Author(s):

Jorge Martinez ◽

Patricio C. Smith

Keyword(s):

Extracellular Matrix ◽

Type I Collagen ◽

Cell Metabolism ◽

Breast Tumors ◽

Extracellular Matrix Remodeling ◽

Type I ◽

Matrix Remodeling ◽

Desmoplastic Reaction ◽

The Impact

Desmoplastic tumors correspond to a unique tissue structure characterized by the abnormal deposition of extracellular matrix. Breast tumors are a typical example of this type of lesion, a property that allows its palpation and early detection. Fibrillar type I collagen is a major component of tumor desmoplasia and its accumulation is causally linked to tumor cell survival and metastasis. For many years, the desmoplastic phenomenon was considered to be a reaction and response of the host tissue against tumor cells and, accordingly, designated as “desmoplastic reaction”. This notion has been challenged in the last decades when desmoplastic tissue was detected in breast tissue in the absence of tumor. This finding suggests that desmoplasia is a preexisting condition that stimulates the development of a malignant phenotype. With this perspective, in the present review, we analyze the role of extracellular matrix remodeling in the development of the desmoplastic response. Importantly, during the discussion, we also analyze the impact of obesity and cell metabolism as critical drivers of tissue remodeling during the development of desmoplasia. New knowledge derived from the dynamic remodeling of the extracellular matrix may lead to novel targets of interest for early diagnosis or therapy in the context of breast tumors.

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