Acute ethanol increases angiogenic growth factor gene expression in rat skeletal muscle

2002 ◽  
Vol 92 (3) ◽  
pp. 1176-1182 ◽  
Author(s):  
Timothy P. Gavin ◽  
Peter D. Wagner
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Growth Factor ◽  
Acute Exercise ◽  
Muscle Growth ◽  
Angiogenic Growth Factor ◽  
Factor Gene ◽  
Skeletal Muscle Growth ◽  
Growth Factor Gene ◽  
Exercise Induced

Moderate ethanol consumption demonstrates a protective effect against cardiovascular disease and improves insulin sensitivity, possibly through angiogenesis. We investigated whether 1) ethanol would increase skeletal muscle growth factor gene expression and 2) the effects of ethanol on skeletal muscle growth factor gene expression were independent of exercise-induced growth factor gene expression. Female Wistar rats were used. Four groups (saline + rest; saline + exercise; 17 mmol/kg ethanol + rest; and 17 mmol/kg ethanol + exercise) were used to measure the growth factor response to acute exercise and ethanol administration. Vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1), basic fibroblast growth factor (bFGF), Flt-1, and Flk-1 mRNA were analyzed from the left gastrocnemius by quantitative Northern blot. Ethanol increased VEGF, TGF-β1, bFGF, and Flt-1 mRNA at rest and after acute exercise. Ethanol increased resting Flk-1 mRNA. Ethanol increased bFGF mRNA independently of exercise. These findings suggest that 1) ethanol can increase skeletal muscle angiogenic growth factor gene expression and 2) the mechanisms responsible for the ethanol-induced increases in VEGF, TGFβ1, and Flt-1 mRNA appear to be different from those responsible for exercise-induced regulation. Therefore, these results provide evidence in adult rat tissue that the protective cardiovascular effects of moderate ethanol consumption may result in part through the increase of angiogenic growth factors.

Effect of NO, vasodilator prostaglandins, and adenosine on skeletal muscle angiogenic growth factor gene expression

1999 ◽  
Vol 86 (5) ◽  
pp. 1513-1518 ◽  
Author(s):  
H. Benoit ◽  
M. Jordan ◽  
H. Wagner ◽  
P. D. Wagner
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Regulation Of Gene Expression ◽  
Transforming Growth Factor Β1 ◽  
Angiogenic Growth Factor ◽  
Factor Gene ◽  
Growth Factor Gene

Exercise training results in several muscle adaptations, one of which is angiogenesis. Acutely, exercise leads to release of nitric oxide, prostacyclin (PGI2), and adenosine (A) in the skeletal muscles. In this paper, we asked whether any of these locally released vasodilators, as well as other known dilator prostaglandins (PGE1 and PGE2), have the potential to increase angiogenic growth factor gene expression in resting skeletal muscle. Seven groups of 5–7 female Wistar rats (age 8–12 wk, weight 250 ± 10 g) were anesthetized and instrumented for carotid artery pressure and electromagnetic femoral artery blood flow measurement. One group acted as control while the other groups each received one of the following six agents by constant arterial infusion (dose in μg/min): A (200), nitroprusside (NP, 4.2), acetylcholine (100), PGE1 (1.9), PGE2 (1.7), and PGI2 (1.7). Each agent reduced peripheral vascular resistance to a similar extent (at least twofold). Densitometric mRNA/18S levels for vascular endothelial growth factor (VEGF) were increased 50% by NP and acetylcholine, were unaffected by PGE1 and PGE2, and were reduced 40% by PGI2. For basic fibroblast growth factor, only PGI2 had any effect, reducing mRNA/18S ∼25%. For transforming growth factor-β1, A, NP, and PGE1 led to reduced mRNA/18S, whereas PGE2 slightly increased mRNA/18S. For the principal putative angiogenic growth factor, VEGF, these data suggest that naturally secreted vasodilators in contracting skeletal muscle could be involved in regulation of gene expression, namely, nitric oxide in a positive and PGI2 in a negative direction.

IMPACT OF CARDIOPLEGIC ARREST ON MYOCARDIAL ANGIOGENIC GROWTH FACTOR GENE EXPRESSION

2004 ◽  
Vol 13 (3) ◽  
pp. 126-127
Author(s):  
Uwe Fischer ◽  
Charles Cox ◽  
Steven Allen ◽  
Wilhelm Bloch ◽  
Uwe Mehlhorn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Cardioplegic Arrest ◽  
Angiogenic Growth Factor ◽  
Factor Gene ◽  
Growth Factor Gene

Activation of insulin-like growth factor gene expression during work-induced skeletal muscle growth

1990 ◽  
Vol 259 (1) ◽  
pp. E89-E95 ◽  
Author(s):  
D. L. DeVol ◽  
P. Rotwein ◽  
J. L. Sadow ◽  
J. Novakofski ◽  
P. J. Bechtel
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Growth Factor ◽  
Muscle Growth ◽  
Experimental Period ◽  
Mrna Levels ◽  
Insulin Like Growth Factor ◽  
Muscle Weight ◽  
Skeletal Muscle Growth ◽  
Igf I

We have investigated the hypothesis that there is local regulation of insulin-like growth factor (IGF) gene expression during skeletal muscle growth. Compensatory hypertrophy was induced in the soleus, a predominantly slow-twitch muscle, and plantaris, a fast-twitch muscle, in 11- to 12-wk-old female Wistar rats by unilateral cutting of the distal gastrocnemius tendon. Animals were killed 2, 4, or 8 days later, and muscles of the nonoperated leg served as controls. Muscle weight increased throughout the experimental period, reaching 127% (soleus) or 122% (plantaris) of control values by day 8. In both growing muscles, IGF-I mRNA, quantitated by a solution-hybridization nuclease-protection assay, rose by nearly threefold on day 2 and remained elevated throughout the experimental period. IGF-II mRNA levels also increased over controls. A more dramatic response was seen in hypophysectomized rats, where IGF-I mRNA levels rose by 8- to 13-fold, IGF-II values by 3- to 7-fold, and muscle mass increased on day 8 to 149% (soleus) or 133% (plantaris) of the control contralateral limb. These results indicate that signals propagated during muscle hypertrophy enhance the expression of both IGF genes, that modulation of IGF-I mRNA levels can occur in the absence of growth hormone, and that locally produced IGF-I and IGF-II may play a role in skeletal muscle growth.

scholarly journals Fat Depot-Specific Differences in Angiogenic Growth Factor Gene Expression and Its Relation to Adipocyte Size in Cattle

2010 ◽  
Vol 72 (8) ◽  
pp. 991-997 ◽  
Author(s):  
Tomoya YAMADA ◽  
Shin-ichi KAWAKAMI ◽  
Naoto NAKANISHI
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Adipocyte Size ◽  
Angiogenic Growth Factor ◽  
Factor Gene ◽  
Growth Factor Gene ◽  
Fat Depot

Effect of short-term exercise training on angiogenic growth factor gene responses in rats

2001 ◽  
Vol 90 (4) ◽  
pp. 1219-1226 ◽  
Author(s):  
Timothy P. Gavin ◽  
Peter D. Wagner
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Exercise Training ◽  
Citrate Synthase ◽  
Short Term ◽  
Vegf Mrna ◽  
Oxidative Potential ◽  
Factor Gene ◽  
Growth Factor Gene ◽  
Exercise Induced

We investigated whether 1) 5 days of exercise training would reduce the acute exercise-induced increase in skeletal muscle growth factor gene expression; and 2) reductions in the increase in growth factor gene expression in response to short-term exercise training would be coincident with increases in skeletal muscle oxidative potential. Female Wistar rats were used. Six groups (rest; exercise for 1–5 consecutive days) were used to measure the growth factor response through the early phases of an exercise training program. Vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1), and basic fibroblast growth factor (bFGF) mRNA were analyzed from the left gastrocnemius by quantitative Northern blot. Citrate synthase activity was analyzed from the right gastrocnemius. VEGF and TGF-β1 mRNA increased after each of 5 days of exercise training, whereas exercise on any day did not increase bFGF mRNA. On day 1, the VEGF mRNA response was significantly greater than on days 2–5. However, the reduced increase in VEGF mRNA observed on days 2–5 was not coincident with increases in citrate synthase activity. These findings suggest that, in skeletal muscle, 1) VEGF and TGF-β1 mRNA are increased through 5 days of exercise training and 2) the reduced exercise-induced increase in VEGF mRNA responses on days 2–5 does not result from increases in oxidative potential.

Activation of AP-1 and SP1 correlates with wound growth factor gene expression in glucan-treated human fibroblasts

2002 ◽  
Vol 2 (8) ◽  
pp. 1163-1172 ◽  
Author(s):  
Duo Wei ◽  
David Williams ◽  
William Browder
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Human Fibroblasts ◽  
Factor Gene ◽  
Growth Factor Gene

Connective tissue growth factor gene expression in goat endometrium during estrous cycle and early pregnancy

2020 ◽  
Vol 153 ◽  
pp. 85-90
Author(s):  
Hao-Nan Yu ◽  
Xiu-Meng Li ◽  
Li-Li Kong ◽  
Jie Ren ◽  
Hao Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Connective Tissue ◽  
Estrous Cycle ◽  
Connective Tissue Growth Factor ◽  
Early Pregnancy ◽  
Tissue Growth ◽  
Factor Gene ◽  
Growth Factor Gene

scholarly journals Depolarizing stimuli regulate nerve growth factor gene expression in cultured hippocampal neurons.

1991 ◽  
Vol 88 (14) ◽  
pp. 6289-6292 ◽  
Author(s):  
B. Lu ◽  
M. Yokoyama ◽  
C. F. Dreyfus ◽  
I. B. Black
Keyword(s):  
Gene Expression ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Hippocampal Neurons ◽  
Nerve Growth ◽  
Factor Gene ◽  
Growth Factor Gene ◽  
Cultured Hippocampal Neurons

scholarly journals Connective Tissue Growth Factor Gene Expression in Tissue Sections From Localized Scleroderma, Keloid, and Other Fibrotic Skin Disorders

1996 ◽  
Vol 106 (4) ◽  
pp. 729-733 ◽  
Author(s):  
Atsuyuki Igarashi ◽  
Kiyoko Nashiro ◽  
Kanako Kikuchi ◽  
Shinichi Sato ◽  
Hironobu Ihn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth ◽  
Localized Scleroderma ◽  
Skin Disorders ◽  
Factor Gene ◽  
Tissue Sections ◽  
Growth Factor Gene
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