RP - HPLC Method for Determination of Piperine fromPiper longumLinn. andPiper nigrumLinn

Piper longumLinn. andPiper nigrumLinn. are used as spices and medicines. Quantitative determination of piperine was undertaken to provide an easy and simple analytical method, which can be used as a routine quality control method. RP-HPLC was performed using methanol and water as mobile phase. The detection and quantification was performed at a wavelength of 345 nm. Linearity of detector response for piperine was between the concentrations 0.005% to 0.1%. The correlation coefficient obtained for the linearity was 0.998. The assay value of piperine for fruit and root ofP. longumwas found to be 0.879% and 0.31%. The assay value of piperine for fruit ofP. nigrumwas 4.5%. The recovery value of standard piperine was 99.4%. Low value of standard deviation and coefficient of variation are indicative of high precision of the method.

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NEW VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF RIZATRIPTAN BENZOATE IN BULK AND PHARMACEUTICAL DOSAGE FORM

INDIAN DRUGS ◽

10.53879/id.51.12.10213 ◽

2014 ◽

Vol 51 (12) ◽

pp. 32-36

Author(s):

T. Vishalakhi ◽

◽

S. K Kumar ◽

K Sujana ◽

P Rani

Keyword(s):

Mobile Phase ◽

Dosage Form ◽

Hplc Method ◽

Detector Response ◽

Mobile Phase Flow Rate ◽

Pharmaceutical Dosage Form ◽

Rizatriptan Benzoate ◽

Rp Hplc ◽

Bulk Drug

A simple validated RP HPLC method for the estimation of rizatriptan benzoate in pharmaceutical dosage form and bulk was developed for routine analysis. This method was developed by selecting Agilent TC C18 (250 x 4.6 mm, 5 μ) column as stationary phase and acrylonibrile:water (45:55), pH adjusted to 3, as mobile phase. Flow rate of mobile phase was maintained at 4: 1 mL/min at ambient temperature throughout the experiment. Quantification was achieved with ultraviolet (DAD) detection at 220 nm. The retention time obtained for rizatriptan was 2.8 min. The detector response was linear in the concentration range of 2-25μg/mL. This method was validated and shown to be specific, sensitive, precise, linear, accurate, rugged and robust. Hence, this method can be applied for routine quality control of rizatriptan benzoate in dosage forms as well as in bulk drug.

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A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora

Chromatography Research International ◽

10.1155/2012/162302 ◽

2012 ◽

Vol 2012 ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

Harish C. Upadhyay ◽

Ram K. Verma ◽

Santosh K. Srivastava

Keyword(s):

Quality Control ◽

Flow Rate ◽

Control Method ◽

Hplc Method ◽

Assay Method ◽

Good Linearity ◽

Rp Hplc ◽

Quality Control Method ◽

Calibration Curves

A rapid, sensitive, and reproducible RP-HPLC method was developed for the determination of two constituents in Ammannia multiflora, namely, tetralone-4-O-β-D-glucopyranoside (1) and 4-hydroxy-α-tetralone (2). The samples were separated on a Spherisorb ODS2 column (250×4.6 mm, i.d., 10 μm) and binary elution of water and methanol (2 : 3) with flow rate of 0.8 mL/min at λ 254 nm. The LOD and LOQ were found 0.05 and 0.18 μg/mL for compound 1 and 0.06 and 0.18 μg/mL for compound 2, respectively. All calibration curves showed good linearity (r2>0.999) within test ranges for both the analytes. The RSD values for intra-and inter-day precisions were less than 1.1%. The successful application of the developed method on five different samples revealed an average 0.0206% and 0.7636% (w/w) of compounds 1 and 2, respectively in A. multiflora indicating that the developed LC assay method may be readily utilized as a quality control method for the plant.

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A comparative technique using as Joint studying to prove structure and determination the Quantitative estimation of organic compound (Irbesartan) as drug in multicomponent tablet form

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v9i3.6 ◽

2019 ◽

Vol 9 (o3) ◽

Author(s):

Imad Tarek Hanoon ◽

Abed Mohammed Daheir AL-Joubory 2 ◽

Marwa Mohamed Saied 3

Keyword(s):

Mobile Phase ◽

Chemical Structure ◽

Quantitative Estimation ◽

Potassium Phosphate ◽

Hplc Method ◽

Pharmaceutical Dosage Forms ◽

Tablet Form ◽

Rp Hplc ◽

Percent Recovery

A simple , specific, accurate and precise RP-HPLC method was developed for determination of Irbesartan (IRB) in pharmaceutical dosage forms in tablets products and sachet using symmetry (L 1 ) column at 30°C . The signal was detected at 225 nm. A mobile phase dissolve 0.5 g of buffer potassium phosphate in 100 ml distilled water and adjust pH 2.7 , methanol and acetonitrile at ratio (40 :30 :30 ) . and flow rate 1.2ml/min -1 at pH=7.2 a mobile phase The percent recovery was detected 101 % and the linearity of concentration was 10-50 µg.ml -1 and supported this method by using (FT.I.R.) spectrum method for organic spectrophotometer to prove the chemical structure of this drug and some physical properties . we are obtained the result is identical of other literature . The proposed method was applied successfully for determination of the IRB in tablets products.

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Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole

Separations ◽

10.3390/separations8010005 ◽

2021 ◽

Vol 8 (1) ◽

pp. 5

Author(s):

Mohd Afzal ◽

Mohd. Muddassir ◽

Abdullah Alarifi ◽

Mohammed Tahir Ansari

Keyword(s):

Flow Rate ◽

Mobile Phase ◽

Limit Of Detection ◽

Hplc Method ◽

Box Behnken Design ◽

Rp Hplc ◽

System Suitability ◽

Method Optimization ◽

Key Variables

A highly specific, accurate, and simple RP-HPLC technique was developed for the real-time quantification of domperidone (DOMP) and lansoprazole (LANS) in commercial formulations. Chromatographic studies were performed using a Luna C8(2), 5 μm, 100Å, column (250 × 4.6 mm, Phenomenex) with a mobile phase composed of acetonitrile/2 mM ammonium acetate (51:49 v/v), pH 6.7. The flow rate was 1 mL·min−1 with UV detection at 289 nm. Linearity was observed within the range of 4–36 µg·mL−1 for domperidone and 2–18 µg·mL−1 for lansoprazole. Method optimization was achieved using Box-Behnken design software, in which three key variables were examined, namely, the flow rate (A), the composition of the mobile phase (B), and the pH (C). The retention time (Y1 and Y3) and the peak area (Y2 and Y4) were taken as the response parameters. We observed that slight alterations in the mobile phase and the flow rate influenced the outcome, whereas the pH exerted no effect. Method validation featured various ICH parameters including linearity, limit of detection (LOD), accuracy, precision, ruggedness, robustness, stability, and system suitability. This method is potentially useful for the analysis of commercial formulations and laboratory preparations.

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Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms

E-Journal of Chemistry ◽

10.1155/2010/470785 ◽

2010 ◽

Vol 7 (3) ◽

pp. 807-812 ◽

Cited By ~ 2

Author(s):

Vanita Somasekhar ◽

D. Gowri Sankar

Keyword(s):

Limit Of Detection ◽

Hplc Method ◽

Detector Response ◽

Reverse Phase Hplc ◽

Limit Of Quantification ◽

Pharmaceutical Dosage Forms ◽

Rp Hplc ◽

Accuracy And Precision ◽

Development And Validation

A reverse phase HPLC method is described for the determination of esmolol hydrochloride in bulk and injections. Chromatography was carried on a C18column using a mixture of acetonitrile, 0.05 M sodium acetate buffer and glacial acetic acid (35:65:3 v/v/v) as the mobile phase at a flow rate of 1 mL/min with detection at 275 nm. The retention time of the drug was 4.76 min. The detector response was linear in the concentration of 1-50 μg/mL. The limit of detection and limit of quantification was 0.614 and 1.86 μg/mL respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of esmolol hydrochloride in bulk and injections.

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RP-HPLC Determination of Raloxifene in Pharmaceuticl Tablets

E-Journal of Chemistry ◽

10.1155/2006/713569 ◽

2006 ◽

Vol 3 (1) ◽

pp. 60-64 ◽

Cited By ~ 6

Author(s):

P. Venkata Reddy ◽

B. Sudha Rani ◽

G. Srinu Babu ◽

J. V. L. N. Seshagiri Rao

Keyword(s):

Flow Rate ◽

Retention Time ◽

Mobile Phase ◽

Dosage Forms ◽

Hplc Method ◽

Reverse Phase Hplc ◽

Reverse Phase ◽

Pharmaceutical Dosage Forms ◽

Rp Hplc

A reverse phase HPLC method is developed for the determination of Raloxifene in pharmaceutical dosage forms. Chromatography was carried out on an inertsil C18 column using a mixture of acetonitrile and phosphate buffer (30:70 v/v) as the mobile phase at a flow rate of 1 mL/min. Detection was carried out at 290 nm .The retention time of the drug was 10.609 min. The method produced linear responses in the concentration range of 0.5-200 µg/mL of Raloxifene. The method was found to be applicable for determination of the drug in tablets.

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Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation

E-Journal of Chemistry ◽

10.1155/2010/487197 ◽

2010 ◽

Vol 7 (1) ◽

pp. 246-252 ◽

Cited By ~ 7

Author(s):

S. K. Patro ◽

S. K. Kanungo ◽

V. J. Patro ◽

N. S. K. Choudhury

Keyword(s):

Linear Response ◽

Mobile Phase ◽

Hplc Method ◽

Forced Degradation ◽

Control Analysis ◽

Solution Stability ◽

Stability Indicating ◽

Quality Control Analysis ◽

Rp Hplc

A simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of valsartan in pure and tablet forms. The method showed a linear response for concentrations in the range of 50-175 µg/mL using 0.01 M NH4H2PO4(pH 3.5) buffer: methanol [50:50] as the mobile phase with detection at 210 nm and a flow rate of 1 mL/min and retention time 11.041 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis.

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PREPARATION OF POLYHERBAL DISPERSIBLE TABLET AND EVALUATION BY RP-HPLC

INDIAN DRUGS ◽

10.53879/id.57.04.11931 ◽

2020 ◽

Vol 57 (04) ◽

pp. 32-39

Author(s):

Divya Hegde ◽

◽

Kirankumar Hullatti ◽

Vishakha Parab Gaonkar ◽

Uday Kumar Bolmal

Keyword(s):

Raw Materials ◽

Chemical Properties ◽

Hplc Method ◽

Piper Nigrum ◽

Piper Longum ◽

Rp Hplc ◽

Physico Chemical ◽

Poor Patient Compliance ◽

Dispersible Tablet ◽

Acceptance Range

The aim of the present study was to convert an Ayurvedic formulation, “trikatu churna”, into a polyherbal dispersible tablet. Standardization parameters were performed for raw materials, trikatu churna and its extract. The standardized extract was further used for formulation of the dispersible tablet. The evaluation of raw materials, trikatu churna, trikatu extract and dispersible tablet was done using preliminary phytochemical and physico-chemical tests. The pre- and post-compression parameters for polyherbal dispersible tablet formulation were within the acceptance range. The piperine content in Piper nigrum, Piper longum, trikatu extract and formulation was found to be 2.884mg/mL, 0.515mg/gm, 0.303mg/gm and 0.357mg/gm, respectively. The developed RP-HPLC method was found to be precise, accurate and reproducible for the estimation of piperine in raw materials and dispersible tablet. It can be concluded that the prepared dispersible tablet has overcome the disadvantages of churna, such as poor patient compliance and pungent taste, without altering its dose and chemical properties.

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RP-HPLC AND HPTLC STABILITY INDICATING ASSAY METHODS FOR IVERMECTIN IN BULK AND TABLET DOSAGE FORM

INDIAN DRUGS ◽

10.53879/id.55.03.11143 ◽

2018 ◽

Vol 55 (03) ◽

pp. 32-42

Author(s):

G. P Wani ◽

◽

S. B Jadhav

Keyword(s):

Mobile Phase ◽

Stress Testing ◽

Hplc Method ◽

Rp Hplc ◽

Assay Methods ◽

Photo Stability ◽

Tablet Dosage ◽

Hptlc Method ◽

Alkaline Oxidation

Simple, rapid, precise, accurate RP-HPLC and HPTLC methods have been developed and validated for ivermectin in bulk and its marketed formulation. RP-HPLC method for drug was achieved on Grace C18 (250 mm X 4.6 ID, Particle size; 5 μ) column using mobile phase acetonitrile: 10 mM phosphate buffer (95:05 v/v) pH adjusted to 3 with o-phosphoric acid. Detection of drug was done at 245 nm. The retention time was found to be 5.83 min. HPTLC method for ivermectin was accomplished on a precoated silica gel aluminium plate 60F-254 (CAMAG Linomat 5), using toluene: methanol: glacial acetic acid (8:2:0.1 v/v/v) as a mobile phase. The densitometric scanning was performed at 245 nm which showed Rf 0.46 for ivermectin. The stress testing of the drug individually was carried out under acidic, alkaline, oxidation, photo-stability and thermal degradation conditions. The proposed methods were successfully applied for the determination of drug in bulk and its marketed formulation.

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DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF BERBERINE AND CURCUMIN IN AN AYURVEDIC FORMULATION

INDIAN DRUGS ◽

10.53879/id.53.08.10621 ◽

2016 ◽

Vol 53 (08) ◽

pp. 48-52

Author(s):

K. P Parekh ◽

◽

A. P. Jadhav

Keyword(s):

Mobile Phase ◽

Herbal Medicines ◽

Hplc Method ◽

Simultaneous Estimation ◽

Quality And Safety ◽

Stability Indicating ◽

Rp Hplc ◽

Water Ph ◽

Development And Validation

A simple, accurate, precise, robust stability indicating RP-HPLC method was developed and validated for simultaneous estimation of berberine and curcumin in an ayurvedic formulation. The two markers were resolved using a C-18 column using as the mobile phase methanol: water (pH 3 adjusted using acetic acid) in the ratio 75:25 V/V at a flow rate of 1mL/min. Retention times of berberine and curcumin were 2.58 ± 0.2 min and 8.5 ± 0.2 min, respectively at 358 nm. Linear response was observed in the concentration range of 2 – 8 ppm for berberine and 5 – 40 ppm for curcumin, with correlation coefficient (r2) of 0.994 and 0.998 for berberine and curcumin, respectively. The developed method was applied for quantitation of markers in marketed and in-house formulations of Gruhadhoomadi Churna. This method can also be used to evaluate formulations containing berberine and curcumin as markers, thus conforming to the need of ensuring quality and safety of herbal medicines.

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