CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF

Objective. To determine whether CD9 expression on human granulosa cells (GCs) and platelets could predict the success of conventional fertilization of human oocytes during in vitro fertilization (IVF).Methods. Thirty women undergoing IVF for nonmale factor infertility participated. Platelets from venous blood and GCs separated from retrieved oocytes were prepared for immunofluorescence. Flow cytometry quantified the percent of GCs expressing CD9, and CD9 surface density on GCs and platelets. Fertilization rate was determined for the total number of oocytes, and the number of mature oocytes per patient. Correlations tested for significant relationships (P<.05) between fertilization rates and CD9 expression.Results. CD9 surface density on human GCs is inversely correlated with fertilization rate of oocytes (P=.04), but the relationship was weak.Conclusion. More studies are needed to determine if CD9 expression on GCs would be useful for predicting conventional fertilization success during IVF.

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Culture of human granulosa cells from an in vitro fertilization program: effects of extracellular matrix on morphology and cyclic adenosine 3′,5′ monophosphate production**Supported by a grant from the Rockefeller Foundation and by grant CA 30289 from the National Institutes of Health.

Fertility and Sterility ◽

10.1016/s0015-0282(16)49596-8 ◽

1986 ◽

Vol 46 (3) ◽

pp. 514-517 ◽

Cited By ~ 22

Author(s):

Anchel Furman ◽

Siegfried Rotmensch ◽

Jehoshua Dor ◽

Annette Venter ◽

Shlomo Mashiach ◽

...

Keyword(s):

Extracellular Matrix ◽

In Vitro Fertilization ◽

Granulosa Cells ◽

Rockefeller Foundation ◽

National Institutes Of Health ◽

Fertilization Program ◽

Vitro Fertilization ◽

Human Granulosa Cells ◽

Program Effects

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Direct effect of gonadotropin-releasing hormone agonist and antagonist on the growth, apoptosis and steroidogenesis in human granulosa cells from women undergoing in vitro fertilization

Fertility and Sterility ◽

10.1016/j.fertnstert.2004.07.781 ◽

2004 ◽

Vol 82 ◽

pp. S290

Author(s):

A.M. Vitale ◽

D. Abramovich ◽

M.C. Peluffo ◽

G. Meresman ◽

M. Tesone

Keyword(s):

In Vitro Fertilization ◽

Direct Effect ◽

Granulosa Cells ◽

Gonadotropin Releasing Hormone ◽

Vitro Fertilization ◽

Releasing Hormone ◽

Human Granulosa Cells ◽

Gonadotropin Releasing Hormone Agonist ◽

Agonist And Antagonist

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Stimulation of Apoptosis in Human Granulosa Cells from in Vitro Fertilization Patients and Its Prevention by Dexamethasone: Involvement of Cell Contact and Bcl-2 Expression

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.87.7.8676 ◽

2002 ◽

Vol 87 (7) ◽

pp. 3441-3451 ◽

Cited By ~ 40

Author(s):

Ravid Sasson ◽

Abraham Amsterdam

Keyword(s):

In Vitro Fertilization ◽

Actin Cytoskeleton ◽

Gap Junctions ◽

Granulosa Cells ◽

Connexin 43 ◽

Pronounced Increase ◽

Vitro Fertilization ◽

Human Granulosa Cells ◽

Progesterone Production

Human granulosa cells obtained from in vitro fertilization patients are highly luteinized, but can still be stimulated by LH/cAMP for production of progesterone. This stimulation involved enhancement of apoptosis. Incubation of the cells with dexamethasone (Dex) reduced the apoptotic incidence compared with nontreated cells and completely abolished the increase in apoptosis stimulated by LH or forskolin, concomitantly with a pronounced increase in progesterone production. Organization of the actin cytoskeleton was dramatically reduced after LH/forskolin stimulation. In contrast, Dex prevented disorganization of the actin filament networks. LH and forskolin also decreased the organization of gap junctions, which could be prevented by Dex. However, the intracellular level of connexin 43 was elevated in the presence of LH, forskolin, and Dex. Endogenous levels of the survival gene protein Bcl-2 were significantly elevated in all cultures treated with Dex compared with either nonstimulated cultures or cultures stimulated with LH and forskolin. Our data suggest that LH/cAMP can stimulate steroidogenesis even during the initial stage of apoptosis of human granulosa cells, whereas Dex, which blocks apoptosis, could further elevate progesterone production. Moreover, the integrity of gap junctions and the actin cytoskeleton as well as elevated levels of Bcl-2 may play an important role in the suppression of apoptosis of human granulosa cells.

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Effect of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells

Acta Endocrinologica ◽

10.1530/acta.0.1290165 ◽

1993 ◽

Vol 129 (2) ◽

pp. 165-168 ◽

Cited By ~ 15

Author(s):

József Bódis ◽

Hans R Tinneberg ◽

Attila Török ◽

Philippe Cledon ◽

Volker Hanf ◽

...

Keyword(s):

Granulosa Cells ◽

Direct Action ◽

Serum Free Medium ◽

Free Medium ◽

Vitro Fertilization ◽

Serum Free ◽

Human Granulosa Cells ◽

Estradiol Secretion ◽

Cells Cultured

The aim of this study was to explore the direct action of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Progesterone and estradiol production was measured in the presence and absence of noradrenaline, dopamine or propranolol using radioimmunoassays; statistical analysis was performed by analysis of variance and Newman-Keul's multiple range test. Twenty-six women aged 31±3 years undergoing in vitro fertilization and embryo transfer for infertility treatment at University Women's Hospital, University of Tübingen, Germany, took part in this study. Noradrenaline significantly inhibited progesterone production by human granulosa cells in a dose-related manner at a concentration of 10−4–10−6 mol/l. Dopamine significantly stimulated estradiol secretion by granulosa cells in an inverse dose-related manner. Both effects were blocked by propranolol. The results suggest that catecholaminergic actions switch over the steroid production of human granulosa cells cultured in serum-free medium from progesterone to estradiol.

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The effects of dehydroepiandrosterone (DHEA) on cultured human granulosa cells from postovulatory human follicles after in vitro fertilization (IVF)

Fertility and Sterility ◽

10.1016/j.fertnstert.2014.07.924 ◽

2014 ◽

Vol 102 (3) ◽

pp. e272

Author(s):

Y.-G. Wu ◽

H.-J. Lee ◽

D.H. Barad ◽

V.A. Kushnir ◽

E. Lazzaroni-Tealdi ◽

...

Keyword(s):

In Vitro Fertilization ◽

Granulosa Cells ◽

Vitro Fertilization ◽

Human Granulosa Cells

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Aging attenuates the ovarian circadian rhythm

Journal of Assisted Reproduction and Genetics ◽

10.1007/s10815-020-01943-y ◽

2020 ◽

Author(s):

Ziru Jiang ◽

Kexin Zou ◽

Xia Liu ◽

Hangchao Gu ◽

Yicong Meng ◽

...

Keyword(s):

Circadian Rhythm ◽

Circadian Clock ◽

Granulosa Cells ◽

Clock Genes ◽

University Hospital ◽

Vitro Fertilization ◽

Human Granulosa Cells ◽

Circadian Clock Genes ◽

Young Mice

Abstract Objective To study the effect of aging on ovarian circadian rhythm. Design Human and animal study. Setting University hospital and research laboratory. Patients/animals Human granulosa cells were obtained by follicular aspiration from women undergoing in vitro fertilization (IVF), and ovarian and liver tissues were obtained from female C57BL/6 mice. Intervention(s) None. Main outcome measure(s) Expression of circadian genes in young and older human granulosa cells and circadian rhythm in ovaries and livers of young and older mice. Result(s) All examined circadian clock genes in human granulosa cells showed a downward trend in expression with aging, and their mRNA expression levels were negatively correlated with age (P < 0.05). Older patients (≥ 40 years of age) had significantly reduced serum anti-Müllerian hormone (AMH) levels. Except for Rev-erbα, all other examined circadian clock genes were positively correlated with the level of AMH (P < 0.05). The circadian rhythm in the ovaries of older mice (8 months) was changed significantly relative to that in ovaries of young mice (12 weeks), although the circadian rhythm in the livers of older mice was basically consistent with that of young mice. Conclusion(s) Lower ovarian reserve in older women is partially due to ovarian circadian dysrhythmia as a result of aging.

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Augmentation by epidermal growth factor of basal and stimulated progesterone production by human luteinized granulosa cells

Journal of Endocrinology ◽

10.1677/joe.0.1210397 ◽

1989 ◽

Vol 121 (2) ◽

pp. 397-402 ◽

Cited By ~ 9

Author(s):

M. C. Richardson ◽

S. C. Gadd ◽

G. M. Masson

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Granulosa Cells ◽

Cultured Cells ◽

Vitro Fertilization ◽

Human Granulosa Cells ◽

Culture Cells ◽

Oocyte Collection ◽

Epidermal Growth

ABSTRACT Human granulosa cells were prepared from follicular aspirates obtained during oocyte collection for in-vitro fertilization. Following several days in culture, cells were washed and then progesterone output was measured in 2-h incubations. After culture for 3 days, incubated cells responded well to human chorionic gonadotrophin (hCG) and prostaglandin (PG) E2 with similar levels of maximum response. Exposure of cultured cells to epidermal growth factor (EGF) for 2 days (days 3–5) led to substantial increases both in basal production and in responses to hCG and PGE2 during subsequent incubations. These effects of EGF were not accompanied by measurable increases in DNA levels in cultures over this time. Results may point to a possible paracrine role for EGF-like factors modulating the activity of cells forming the early corpus luteum. Journal of Endocrinology (1989) 121, 397–402

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Cigarette smoke is associated with altered expression of antioxidant enzymes in granulosa cells from women undergoing in vitro fertilization

Zygote ◽

10.1017/s0967199417000132 ◽

2017 ◽

Vol 25 (3) ◽

pp. 296-303 ◽

Cited By ~ 7

Author(s):

Maria Cristina Budani ◽

Erminia Carletti ◽

Gian Mario Tiboni

Keyword(s):

Antioxidant Enzymes ◽

Cigarette Smoke ◽

Granulosa Cells ◽

Implantation Rate ◽

Live Birth Rate ◽

Fertilization Rate ◽

Mrna Levels ◽

Altered Expression ◽

Vitro Fertilization

SummaryThis study was undertaken to evaluate whether cigarette smoke is associated with changes in the expression of antioxidant enzymes in granulosa cells of women undergoing IVF treatments. For this aim, the expression of three antioxidant enzymes (SOD1, SOD2 and catalase) in non-smokers (n = 20) and smokers (n = 20) was analyzed. There was a statistically significant overexpression of SOD2 and catalase mRNA levels in smokers in comparison with non-smokers. Cigarette smoking was associated with a lower fertilization rate, implantation rate and pregnancy rate in comparison with non-smokers. There was no effect on retrieved oocytes number, metaphase II oocytes number, quality of embryos transferred and live birth rate. These findings suggest that cigarette smoke initiates oxidative stress in granulosa cells.

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OXYTOCIN BIOSYNTHESIS IN SERUM-FREE CULTURES OF HUMAN GRANULOSA CELLS

Journal of Endocrinology ◽

10.1677/joe.0.124r005 ◽

1990 ◽

Vol 124 (2) ◽

pp. R5-R8 ◽

Cited By ~ 8

Author(s):

I. plevrakis ◽

C. Clamagirand ◽

G. Pontonnier

Keyword(s):

Granulosa Cells ◽

High Performance ◽

Serum Free Medium ◽

Vitro Fertilization ◽

Serum Free ◽

Human Granulosa Cells ◽

Cell Extracts ◽

Preovulatory Follicles ◽

Follicular Puncture

ABSTRACT Human granulosa cells were collected from preovulatory follicles during follicular puncture for in-vitro fertilization. They were cultured in serum-free medium supplemented with ascorbic acid. Using a combination of high-performance liquid chromatography and radioimmunoassay, the oxytocin material present in the cell extracts and secreted into the medium was identified. When cells were deprived of ascorbate, intermediary forms resulting of the post-translational processing of pro-oxytocin/neurophysin were detected. These data demonstrate that oxytocin biosynthesis occurs in human granulosa cells.

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