scholarly journals Culture Method and PCR for the Detection ofHelicobacter pyloriin Drinking Water in Basrah Governorate Iraq

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
A. A. Al-Sulami ◽  
T. A. A. Al-Edani ◽  
A. A. Al-Abdula
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Culture Method ◽  
Human Infection ◽  
World Health ◽  
Peptic Ulcers ◽  
Biochemical Tests ◽  
Gram Staining ◽  
H Pylori

Helicobacter pyloriis recognized by the World Health Organization to be the primary cause of peptic ulcers, chronic gastritis, and stomach cancer, though the source of human infection is not well understood. One of the problems in understanding the source of human contamination is the difficulty in isolating the organism from the environment. However, the combination of PCR results with those of culturing of 471 drinking water samples can provide a more accurate picture ofH. pyloridetection. In this method 78 presumptiveH. pyloricolonies out of 266 tap water samples were obtained in the preliminary detection on modified Columbia agar (MCUA) slant relying on urease positivity with a rate of 29.3%. However, only 11 out of them were confirmed by Gram staining and biochemical tests reducing the rate to 4.13% whereas only 3 (1.46%) from 205 reverse osmosis (RO) water samples. Furthermore, only 6 (54.5%) out of the 11 isolates from tap water and 1 (33.3%) of the 3 RO isolates were confirmed by 16SrRNA PCR. Thus PCR confirmation reduced the rate to 2.2%. In addition, only 4 (4%) of 100 tap water samples negative forH. pyloriby culture method wereH. pyloripositive by 16SrRNA. Water samples were collected from 24 districts of Basrah Governorate from February–December 2009. The direct recovery ofH. pylorifrom drinking water is both alarming and scientifically exciting in terms of the investigation of its epidemiology.

Bacterial quality of drinking water sources and antimicrobial resistance profile of Enterobacteriaceae in Bahir Dar city, Ethiopia

2014 ◽  
Vol 4 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Bayeh Abera ◽  
Mulugeta Kibret ◽  
Goraw Goshu ◽  
Mulat Yimer
Keyword(s):  
Drinking Water ◽  
Antimicrobial Resistance ◽  
Water Samples ◽  
Multiple Drug Resistance ◽  
Tap Water ◽  
World Health ◽  
Most Probable Number ◽  
Bahir Dar ◽  
Probable Number

A cross-sectional study was conducted to determine the bacterial quality and antimicrobial susceptibility profiles of Enterobacteriaceae from drinking water in Bahir Dar city. A total of 140 water samples were collected in the wet and dry periods from springs (n = 4), reservoirs (n = 10) and private tap water at households (n = 126). Bacteriological analysis of water was conducted using multiple tube method. Overall, 21.4%, 18.6% and 17.8% of drinking water samples had total coliforms (TC), faecal coliforms (FC) and Escherichia coli, respectively. All spring water samples and 29.2% of private tap water had the highest TC load (18 most probable number/100 mL, 95% CI: 100). For FC, 81.4% of the drinking water supplies tested complied with both World Health Organization and Ethiopian Standards. High levels of resistance (98–100%) were observed for ampicillin by Enterobacteriaceae and Pseudomonas aeruginosa. All P. aeruginosa isolates and 20 (66.7%) of E. coli revealed multiple drug resistance. Enterobacteriaceae and P. aeruginosa isolates exhibited high levels of antimicrobial resistance. The bacterial quality of drinking water in Bahir Dar city was poor. Microbial surveillance and monitoring with periodic assessment on physical integrity of the water pipelines need to be undertaken.

scholarly journals Icp-Ms Determination of Trace Metals in Drinking Water Sources in Jazan Area, Saudi Arabia

2017 ◽  
Vol 12 (1) ◽  
pp. 06-17 ◽  
Author(s):  
Mohamed Albratty ◽  
Ismail Arbab ◽  
Hassan Alhazmi ◽  
Ibraheem Attafi ◽  
Abdul Al-Rajab
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Saudi Arabia ◽  
Water Samples ◽  
Research Team ◽  
Tap Water ◽  
Drinking Water Quality ◽  
World Health ◽  
Bottled Drinking Water ◽  
Icp Ms

Requisite reference facts about essential elements in treated and plastic bottled drinking water is usually mentioned on the products. However, more information is sometime crucial regarding trace elements in treated, bottled and other sources of drinking water such as tap water to evaluate their quality. This study is aimed to evaluate drinking water quality in the main governorates (Jazan, Sabya and Abu Arish) of Jazan province located in the South-Western region of Kingdom of Saudi Arabia. This is the first such study carried out in Jazan city so that the research team can come out with valuable recommendations in the issue. The research team has collected a sum of 68 water samples from different sources comprising hospitals (treated tap water), stations for drinking water treatment (large blue bottles of drinking water) and bottled drinking water (purchased from local markets). Using inductively coupled plasma-mass spectrometry (ICP-MS), the concentrations of 20 elements were determined. The physiochemical parameters of water samples were measured. All parameters were within the recommended limits of the World Health Organization (WHO, 2011) and Gulf Cooperation Council Standardization Organization (GSO, 2008). Trace and major elements were found to be below the standard guideline values , except for uranium in some tap water samples. This preliminary study will significantly improve the awareness and knowledge among the society about the drinking water quality in Jazan area.

scholarly journals High frequency of Helicobacter pylori DNA in drinking water in Kermanshah, Iran, during June–November 2012

2013 ◽  
Vol 12 (3) ◽  
pp. 504-512 ◽  
Author(s):  
Alvandi Amirhooshang ◽  
Abiri Ramin ◽  
Aryan Ehsan ◽  
Rezaei Mansour ◽  
Bagherabadi Shahram
Keyword(s):  
Helicobacter Pylori ◽  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Piping System ◽  
Detection Rates ◽  
High Prevalence ◽  
Polymerase Chain ◽  
H Pylori ◽  
Drinking Water Samples

To gain a better understanding of transmission and selecting appropriate measures for preventing the spread of Helicobacter pylori, the aim of this study was to investigate the prevalence of H. pylori in drinking water samples in Kermanshah, Iran. Drinking water samples were collected from around Kermanshah and filtered through 0.45 μm nitrocellulose filters. The bacterial sediment was subjected to DNA extraction and polymerase chain reaction (PCR) for H. pylori detection using newly designed primers targeted at the conserved region of the ureC gene. The overall detection rates for H. pylori DNA in the water samples were 56% (66/118) with a frequency of 36% (25/70) in tap water samples and 85% (41/48) in wells. The detection limit was 50 bacteria per liter of filtered water and a pure H. pylori DNA copy number of 6 per reaction. Based on the evidence we may suggest that recontamination occurred and H. pylori entered into the water piping system through cracked or broken pipes or was released from established H. pylori biofilms on pipes. In conclusion, a high prevalence of H. pylori was detected in drinking water samples that strengthens the evidence of H. pylori transmission through drinking water.

ECOLOGICAL AND PHYSIOLOGICAL ASSESSMENT OF CHEMICAL COMPOSITION OF DRINKING TAP WATER IN KHANTY-MANSIYSK AND SALEKHARD

2020 ◽  
pp. 159-167
Author(s):  
V.V. Lapenko ◽  
L.N. Bikbulatova ◽  
E.M. Ternikova
Keyword(s):  
Drinking Water ◽  
Chemical Composition ◽  
Water Samples ◽  
Tap Water ◽  
Iron Concentration ◽  
Absorption Spectrometry ◽  
Northern Region ◽  
Transport Processes ◽  
Magnesium Concentration ◽  
Geochemical Environment

Water is very important for humans, as it is a solvent for metabolic products. Moreover, it is necessary for metabolism, biochemical and transport processes. The elemental status in persons depends on the geochemical environment and consumption of bioelements with food and water. The aim of the paper is to conduct a comprehensive assessment of chemical composition of drinking tap water in Khanty-Mansiysk and Salekhard. Materials and Methods. The chemical composition of 100 samples of drinking tap water was analyzed by atomic absorption spectrometry, spectrophotometry and capillary electrophoresis. All in all, there were 50 samples from Khanty-Mansiysk and 50 samples from Salekhard. The results were compared with Sanitary Rules and Norms 2.1.4.1074-01. Results. Drinking tap water in Salekhard contains a significantly higher concentration of iron, which is much above the maximum allowable concentration, if compared to water samples in Khanty-Mansiysk (p=0.03). In the cities under consideration, the water undergoes high-quality reagent-free treatment. However, the deterioration of the water supply networks in Salekhard is 3 times as high as in Khanty-Mansiysk. Calcium and magnesium concentration in water samples from Khanty-Mansiysk is 5.6 and 3.9 times lower than the MAC; in water samples from Salekhard calcium concentration is 6.3 (p=0.008) and magnesium concentration 4.6 (p<0.001) times lower than the MAC. Conclusion. The consumption of ultra-fresh drinking water leads to low intake of bioelements, which are a part of enzymes contributing to the human antioxidant defense and can result in manifestation of cardiovascular diseases. This is especially true for Salekhard with very soft drinking water and high iron concentration, which excess can exhibit prooxidant properties. Keywords: tap water, bioelements, northern region, antioxidants. Вода является важнейшим соединением для человека: необходима в качестве растворителя продуктов метаболизма и протекания обменных, биохимических и транспортных процессов. Элементный статус организма человека зависит от геохимического окружения и поступления биоэлементов с пищей и водой. Цель. Провести комплексную оценку химического состава водопроводной воды городов Ханты-Мансийск и Салехард. Материалы и методы. Методами атомно-абсорбционной спектрометрии, спектрофотометрии и капиллярного электрофореза проанализирован химический состав 100 проб водопроводной воды: по 50 из Ханты-Мансийска и Салехарда. Результаты сравнивали с СанПиН 2.1.4.1074-01. Результаты. В водопроводной воде Салехарда установлена превышающая ПДК и достоверно более высокая концентрация железа сравнительно с водой Ханты-Мансийска (р=0,03). При условии качественной безреагентной водоподготовки в изучаемых городах это обусловлено изношенностью водопроводных сетей в Салехарде, более чем в 3 раза превышающей этот показатель в Ханты-Мансийске. Концентрация кальция и магния в воде Ханты-Мансийска в 5,6 и 3,9 раза ниже ПДК; в воде Салехарда – в 6,3 (р=0,008) и 4,6 (р<0,001) раза ниже ПДК соответственно. Заключение. Употребление ультрапресной питьевой воды на фоне очень малого поступления с водой биоэлементов, входящих в состав ферментов антиоксидантной защиты организма человека, может привести к манифестации кардиоваскулярных заболеваний. Это особенно актуально для г. Салехарда с очень мягкой питьевой водой с повышенным содержанием железа, избыток которого может проявлять прооксидантные свойства. Ключевые слова: водопроводная вода, биоэлементы, северный регион, антиоксиданты.

scholarly journals Diarrhea prevalence in a randomized, controlled prospective trial of point-of-use water filters in homes and schools in the Dominican Republic

2021 ◽  
Vol 49 (1) ◽  
Author(s):  
Nathan Tintle ◽  
Kristin Van De Griend ◽  
Rachel Ulrich ◽  
Randall D. Wade ◽  
Tena M. Baar ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Controlled Trial ◽  
World Health ◽  
Dissolved Metals ◽  
Randomized Controlled ◽  
Point Of Use ◽  
Diarrhea Prevalence ◽  
Clean Drinking Water ◽  
Post Filter

Abstract Background Lack of sustainable access to clean drinking water continues to be an issue of paramount global importance, leading to millions of preventable deaths annually. Best practices for providing sustainable access to clean drinking water, however, remain unclear. Widespread installation of low-cost, in-home, point of use water filtration systems is a promising strategy. Methods We conducted a prospective, randomized, controlled trial whereby 16 villages were selected and randomly assigned to one of four treatment arms based on the installation location of Sawyer® PointONE™ filters (filter in both home and school; filter in home only; filter in school only; control group). Water samples and self-reported information on diarrhea were collected at multiple times throughout the study. Results Self-reported household prevalence of diarrhea decreased from 25.6 to 9.76% from installation to follow-up (at least 7 days, and up to 200 days post-filter installation). These declines were also observed in diarrhea with economic or educational consequences (diarrhea which led to medical treatment and/or missing school or work) with baseline prevalence of 9.64% declining to 1.57%. Decreases in diarrhea prevalence were observed across age groups. There was no evidence of a loss of efficacy of filters up to 200 days post-filter installation. Installation of filters in schools was not associated with decreases in diarrhea prevalence in school-aged children or family members. Unfiltered water samples both at schools and homes contained potential waterborne bacterial pathogens, dissolved heavy metals and metals associated with particulates. All dissolved metals were detected at levels below World Health Organization action guidelines. Conclusions This controlled trial provides strong evidence of the effectiveness of point-of-use, hollow fiber membrane filters at reducing diarrhea from bacterial sources up to 200 days post-installation when installed in homes. No statistically significant reduction in diarrhea was found when filters were installed in schools. Further research is needed in order to explore filter efficacy and utilization after 200 days post-installation. Trial registration ClinicalTrials.gov, NCT03972618. Registered 3 June 2019—retrospectively registered.

Evaluation of Gross Alpha and Beta Activity Concentrations and Annual Effective Dose in Drinking Waters of Misan Province-Iraq Using Low Background Gas Flow Proportional Counter (LB- 4110)

2021 ◽  
Vol 19 (11) ◽  
pp. 22-31
Author(s):  
Sawsan Sh. Fleifil ◽  
Zahraa A. Ismail AL-Sudani
Keyword(s):  
Drinking Water ◽  
Effective Dose ◽  
Water Samples ◽  
Proportional Counter ◽  
Gas Flow ◽  
Annual Effective Dose ◽  
Tap Water ◽  
Beta Activity ◽  
Low Background ◽  
Background Gas

In this study, assessment of levels natural radioactivity in drinking water samples of Misan Province of Iraq was carried out. A total of 33 (Tigris river, station and Tap) water samples collected from eleven places in Misan Province of Iraq. The beta and alpha gross radioactivity of the samples water was measured and an average annual effective dose derived of drinking-water ingestion was estimation utilizing new model a LB-4110 low background gas flow proportional counter. The data indicated that the Beta and Alpha gross activities and annual effective dose in samples did not exceed WHO recommended levels (0.5 Bq/L of Alpha gross, 1.0 Bq/L of Beta gross and 0.1 mSv/y for annual effective dose).

scholarly journals Evaluation of Minerals Content of Drinking Water in Malaysia

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Azrina Azlan ◽  
Hock Eng Khoo ◽  
Mohd Aizat Idris ◽  
Amin Ismail ◽  
Muhammad Rizal Razman
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Mineral Water ◽  
Tap Water ◽  
Fluoride Concentration ◽  
Daily Intake ◽  
Peninsular Malaysia ◽  
Mineral Water Sample ◽  
Geographical Locations ◽  
Iron And Manganese

The drinking and mineral water samples obtained from different geographical locations had concentrations of the selected minerals lower than the standard limits, except for manganese, arsenic, and fluoride. The concentrations of manganese and arsenic in two mineral water samples were slightly higher than the standard international recommended limits. One mineral water sample had a fluoride concentration higher than the standard limits, whereas manganese was not detected in nine drinking and mineral water samples. Most of the selected minerals found in the tap water samples were below the international standard limits, except for iron and manganese. The concentrations of iron and manganese in the tap water samples were higher than the standard limits, which were obtained from one and three of the studied locations, respectively. The potable water obtained from various manufacturers and locations in Peninsular Malaysia is safe for consumption, as the minerals concentrations were below the standard limits prescribed by the Malaysian Food Regulations of 1985. The data obtained may also provide important information related to daily intake of these minerals from drinking water.

scholarly journals Physico-chemical quality of drinking water in the Njoro sub-county in Kenya

2018 ◽  
Vol 8 (3) ◽  
pp. 497-507
Author(s):  
Philip Ruciaka Kirianki ◽  
Edward Muchiri ◽  
Natasha Potgieter
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Water Source ◽  
World Health ◽  
Water Shortages ◽  
Storage Container ◽  
Standard Assessment ◽  
Physico Chemical ◽  
Study Support ◽  
Health Organization

Abstract Njoro sub-county in Kenya suffers from constant water shortages causing the residents to rely on both improved and unimproved water sources in the area. The households in the sub-county also use different household storage containers to store drinking water in times when water is not readily available. This study was therefore undertaken to assess selective physico-chemical parameters of water used by the population for drinking purposes using standard assessment methods. A total of 372 water source samples and 162 storage container water samples were tested over a period of three months. Turbidity (0.70–273.85 NTU), iron (0.7–2.10 mg/L), fluoride (0.15–4.01 mg/L), manganese (0.01–0.37 mg/L), and nitrate (0.09–27.90 mg/L) levels in water samples were generally higher than the Kenya Bureau of Standards (KEBS) and/or the World Health Organization (WHO) water quality recommendations for safe drinkable water. The results from this study support the need for continuous monitoring and treating drinking water at the points of collection and of consumption to minimize the long-term health effects on communities consuming this water.

scholarly journals Development of an Internal Control for Evaluation and Standardization of a Quantitative PCR Assay for Detection of Helicobacter pylori in Drinking Water

2007 ◽  
Vol 73 (22) ◽  
pp. 7380-7387 ◽  
Author(s):  
Keya Sen ◽  
Nancy A. Schable ◽  
Dennis J. Lye
Keyword(s):  
Helicobacter Pylori ◽  
Drinking Water ◽  
Quantitative Pcr ◽  
Water Samples ◽  
Sodium Thiosulfate ◽  
Qpcr Assay ◽  
E Coli ◽  
H Pylori ◽  
Labeled Probe ◽  
Treated Drinking Water

ABSTRACT Due to metabolic and morphological changes that can prevent Helicobacter pylori cells in water from growing on conventional media, an H. pylori-specific TaqMan quantitative PCR (qPCR) assay was developed that uses a 6-carboxyfluorescein-labeled probe (A. E. McDaniels, L. Wymer, C. Rankin, and R. Haugland, Water Res. 39:4808-4816, 2005). However, proper internal controls are needed to provide an accurate estimate of low numbers of H. pylori in drinking water. In this study, the 135-bp amplicon described by McDaniels et al. was modified at the probe binding region, using PCR mutagenesis. The fragment was incorporated into a single-copy plasmid to serve as a PCR-positive control and cloned into Escherichia coli to serve as a matrix spike. It was shown to have a detection limit of five copies, using a VIC dye-labeled probe. A DNA extraction kit was optimized that allowed sampling of an entire liter of water. Water samples spiked with the recombinant E. coli cells were shown to behave like H. pylori cells in the qPCR assay. The recombinant E. coli cells were optimized to be used at 10 cells/liter of water, where they were shown not to compete with 5 to 3,000 cells of H. pylori in a duplex qPCR assay. Four treated drinking water samples spiked with H. pylori (100 cells) demonstrated similar cycle threshold values if the chlorine disinfectant was first neutralized by sodium thiosulfate.

scholarly journals Heavy metal levels in drinking water sources of Chingola District, Zambia

2020 ◽  
Author(s):  
Francis Hamwiinga ◽  
Chisala D. Meki ◽  
Patricia Mubita ◽  
Hikabasa Halwiindi
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Drinking Water ◽  
Ground Water ◽  
Water Samples ◽  
Tap Water ◽  
Water Sources ◽  
Wet Season ◽  
Drinking Water Sources ◽  
Metal Levels

Abstract Background: One of the factors impeding access to safe water is water pollution. Of particular concern is heavy metal contamination of water bodies. This study was aimed at determining the levels of heavy metals in drinking water sources of Chingola District of Zambia. Methods: A cross sectional study was employed. A total of 60 water samples were collected. Thirsty (30) samples were collected in the dry season in the month of October 2016 and another 30 in the wet season in the months of February and March, 2017. For each season 10 water samples were collected from each of the three water sources. i.e. Tap water, Urban ground water sources and Rural ground water sources. Heavy metal analysis was done using Atomic Absorption Spectrophotometer (AAS).Results: This study revealed that the concentrations of Iron, Manganese, Lead, Nickel and Arsenic were beyond maximum permissible levels in various water sources. Combined averages for both dry and wet seasons were as follows: Iron: 2.3, Copper: 0.63, Cobalt: 0.02, Manganese: 0.36, Lead: 0.04, Zinc:3.2, Nickel: 0.03, Arsenic: 0.05. Chromium and Cadmium were below detection limit in all water samples. The median concentrations of iron, arsenic, copper, manganese in drinking water from the Tap, rural and urban ground water sources were different, and this difference was statistically significant (p<0.05). The median concentrations of arsenic, nickel, manganese and cobalt were different between dry and wet season, and this difference was statistically significant (p<0.05).Conclusions: Sources of heavy metals in water seems to be both natural and from human activities. The concentration of heavy metals in different water sources in this study was found to be above the recommended levels. This calls for improvement in water monitoring to protect the health of the public. Therefore, there is need for continuous monitoring of heavy metals in drinking water sources by regulatory authorities.

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