scholarly journals Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Kumar Pranaw ◽  
Surender Singh ◽  
Debjani Dutta ◽  
Surabhi Chaudhuri ◽  
Sudershan Ganguly ◽  
...  
Keyword(s):  
Entomopathogenic Nematode ◽  
Industrial Processes ◽  
Protease Production ◽  
Chemical Components ◽  
Independent Variables ◽  
Protease Enzyme ◽  
Dipotassium Phosphate ◽  
Soya Peptone ◽  
Optimal Medium ◽  
Central Composite

Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (RCCD) using response surface methodology (RSM). The effects of all five chemical components (considered as independent variables), namely tryptone, soya peptone, dextrose, NaCl, and dipotassium phosphate, on protease production (dependent variable) were studied, and it was found that tryptone and dextrose had maximum influence on protease production. The protease production was increased significantly by 66.31% under optimal medium conditions (tryptone—5.71, soya peptone—4.9, dextrose—1.45, NaCl—6.08, and dipotassium phosphate—0.47 in g/L). To best of knowledge, there are no reports on optimization of medium component for protease production by X. indica KB-3 using RSM and their application in fibrinolysis. This study will be useful for industrial processes for production of protease enzyme from X. indica KB-3 for its application in the field of agriculture and medicine.

Cytotoxic Effect of Pseudomonas aurogenosa Protease on Cancer Cells

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Ghanyia J. Shanyoor ◽  
Fatima R. Abdul ◽  
Nehad A. Taher ◽  
Ihsan A. Raheem
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Normal Cell Line ◽  
Protease Enzyme ◽  
Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) and the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method and it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column and the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , and one normal cell line Ref ( Rat embryonic fibroblast ) . The cancer and normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8and 0.16 mg/ml) then incubated for additional 48h at 37C0 and the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andslight toxicity ( 37.12% ) on normal cell line (Ref) in a concentration (0.8mg/ml).

Constraints in Adoption ofModern Farm Machines by Tribal Farmers in Ramgarh District of Jharkhand

2019 ◽  
Vol 6 (03) ◽  
Author(s):  
PK SUNDARAM ◽  
BIKASH SARKAR ◽  
UJJWAL KUMAR ◽  
AP ANURAG ◽  
DK RAGHAV ◽  
...  
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Human Cancer ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Normal Cell Line ◽  
Protease Enzyme ◽  
Tribal Farmers ◽  
Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) andamp; the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method andamp; it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column andamp; the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , andamp; one normal cell line Ref ( Rat embryonic fibroblast ). The cancer andamp; normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8andamp; 0.16 mg/ml) then incubated for additional 48h at 37C 0 andamp; the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andamp;slight toxicity ( 37.12% )on normal cell line (Ref) in a concentration (0.8mg/ml).

Kaempferol Extraction from Cuscuta reflexa using Supercritical Carbon Dioxide and Separation of Kaempferol from the Extracts

2011 ◽  
Vol 7 (4) ◽  
Author(s):  
Pranabendu Mitra ◽  
Kyu -Seob Chang ◽  
Dae-Seok Yoo
Keyword(s):  
Supercritical Co2 ◽  
Extraction Process ◽  
Optimum Conditions ◽  
Supercritical Co2 Extraction ◽  
Independent Variables ◽  
Cuscuta Reflexa ◽  
Optimal Processing ◽  
Layer Chromatography ◽  
Temperature Time ◽  
Central Composite

Kaempferol, a strong antioxidant, was extracted from Cuscuta reflexa (a medicinal plant) using supercritical CO2 and separated using thin-layer chromatography, column chromatography and HPLC analysis. A rotatable central composite design was used to determine the influence of process variables and arrive at optimal processing conditions in the supercritical CO2 extraction process of kaempferol. The kaempferol yield was effectively modelled as a function of the independent variables (temperature, time and pressure). The kaempferol yield increased with the increasing of temperature and time and decreasing of pressure of the supercritical CO2 extraction process. The predicted kaempferol yield at the optimum point was 52.92 µg/g and the optimum conditions were 50.7°C for 132.6 min and 15.9 MPa.

scholarly journals Smart Starch-Gelatin Films Incorporated with Curcumin

2020 ◽  
Vol 36 (6) ◽  
pp. 1088-1095
Author(s):  
Le Thi Bich Nguyet ◽  
Vinh Tien Nguyen
Keyword(s):  
Acetic Acid ◽  
Food Packaging ◽  
Gelatin Film ◽  
Film Properties ◽  
Elongation At Break ◽  
Independent Variables ◽  
Gelatin Films ◽  
Film Forming ◽  
Central Composite Experimental Design ◽  
Central Composite

In this study, we developed a starch-gelatin film incorporated with synthesized curcumin to be used as a pH-sensitive smart material for food packaging. The film-forming mixture contained five components: starch, gelatin, glycerol, acetic acid and curcumin. The interactions of the components and their effects on the film properties were investigated by using response surface methodology with central composite experimental design. The results showed impacts of the contents of these components as independent variables on tensile strength, elongation at break, Young’s modulus and solubility of the films. The contents of starch, gelatin and glycerol significantly affect these properties, while acetic acid and curcumin do not (p<0.05). Also, it was shown that the incorporation of curcumin provided the film with the capacity to sense pH changes from neutral to basic (yellow at pH ≤ 8 and orange-red at pH ≥ 9).

Optimization of Furfural Production from Xylose by RSM Using Chromium Sulfate as Catalyst

2014 ◽  
Vol 881-883 ◽  
pp. 29-34
Author(s):  
Ye Zhang ◽  
Ming Qiang Chen ◽  
Jun Wang
Keyword(s):  
Strong Influence ◽  
Catalyst Loading ◽  
Response Analysis ◽  
Solid Residue ◽  
Independent Variables ◽  
Temperature Time ◽  
Surface Response Analysis ◽  
Central Composite ◽  
Chromium Sulfate ◽  
Furfural Production

Optimization of chromium sulfate catalyzed conversion conditions of xylose into furfural was studied by response surfacemethodology (RSM). A central composite design (CCD) was used to determine the effects of independent variables, including temperature (120-160°C), time (30-150min), catalyst loading (1-5mmol) and moles of xylose (2.5-12.5mmol) on furfural yield, xylose conversion and solid residue. The surface response analysis revealed that temperature, time, catalyst loading and moles of xylose had a strong influence on the furfural yield, whereas moles of xylose was found to be notsignificant for xylose conversion. The solid residue was affected by temperature, time and moles of xylose. The maximumpredicted furfural yield was 45.07% at temperature of 150°C, time of 12min, catalyst loading of 2 mmol and moles of xylose of 10mmol. Under this condition, xylose conversion could be reached 100%.

scholarly journals Optimization of Pectin Extraction from Nephrolepis biserrata Leaves Using Response Surface Methodology

2014 ◽  
Vol 625 ◽  
pp. 920-923 ◽  
Author(s):  
Halifah Pagarra ◽  
Roshanida Abd Rahman ◽  
Rosli Md. Illias ◽  
Nor Azah Ramli
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Central Composite Design ◽  
Galacturonic Acid ◽  
Dry Weight ◽  
Extraction Time ◽  
Extraction Condition ◽  
Degree Of Esterification ◽  
Independent Variables ◽  
Central Composite

A central composite design was employed to optimize the extraction of pectin from Nephrolepis biserrata leaves. The independent variables were pH (1.5 to 2.5), extraction time (60 to 120 minutes) and temperature (60oC to 100oC). The combined effect of these variables on yields of pectin was investigated. The results showed that the yield of extracted pectin ranged from 3.76% to 8.50% (w/w, based on dry weight of Nephrolepis biserrata leaves). The optimum condition for the yield of pectin extraction was predicted at pH (1.5), extraction time (76.25 minutes) and temperature (100oC). Under the optimum condition, the actual pectin yield was 8.18%, which was below the predicted extraction condition of 8.316 %. The characteristics were 47.52% galacturonic acid and 83.71% degree of esterification. Keyword: Nephrolepis biserrata leaves, Extraction, Pectin, RSM, Characterization.

scholarly journals Optimization of Medium Ingredients for Keratinolytic Protease Production by Bacillus licheniformis MZK-03 using Statistical Experimental Designs

1970 ◽  
Vol 24 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Mohammad Moniruzzaman ◽  
Alamgir Rahman ◽  
M Mozammel Hoq
Keyword(s):  
Trace Elements ◽  
Bacillus Licheniformis ◽  
Shake Flask ◽  
Culture Medium ◽  
Protease Production ◽  
Shake Flask Culture ◽  
Flask Culture ◽  
Statistical Approaches ◽  
Central Composite ◽  
Keratinolytic Protease

A culture medium was optimized for the production of keratinolytic protease by a newly isolated strain of Bacillus licheniformis MZK-03 in shake-flask culture. Based on the results of preliminary experiments, feather mill, molasses and trace elements were found to be major variables in keratinolytic protease production. The concentrations of these ingredients were optimized by using two statistical approaches, namely Box-Wilson method and central composite design. The optimized culture medium, finally determined by using the statistical approaches, composed of 0.95% feather mill, 0.12% molasses and 1.44% trace elements. The keratinolytic protease production was increased by approximately 2-fold when the strain was grown in the optimized medium (95.2 U/ml) compared to the un-optimized medium (56.05 U/ml). Keywords: Keratinolytic protease, Optimization, Bacillus licheniformis MZK-03, Statistical designsDOI: http://dx.doi.org/10.3329/bjm.v24i1.1238 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 52-56

scholarly journals Optimization of isopropyl alcohol degradation by microwave-induced catalytic oxidation process

2019 ◽  
Vol 9 (3) ◽  
pp. 213-224
Author(s):  
Quynh Thi Phuong Tran ◽  
Chi-Hsu Hsieh ◽  
Tung-Yu Yang ◽  
Hsin-hsin Tung
Keyword(s):  
Hydrogen Peroxide ◽  
Catalytic Oxidation ◽  
Working Conditions ◽  
Isopropyl Alcohol ◽  
Oxidation Process ◽  
Removal Rate ◽  
Response Variability ◽  
Manufacturing Industries ◽  
Independent Variables ◽  
Central Composite

Abstract Isopropyl alcohol (IPA) is a common waste solvent from the semiconductor and optoelectronic manufacturing industries. The current study assesses the feasibility of microwave-induced catalytic oxidation process for synthetic IPA wastewater. The effect of three independent variables, including oxidant (hydrogen peroxide), initial IPA concentration, and dosage of catalyst (granular activated carbon, GAC) on the IPA removal efficiency, were investigated and optimized by response surface methodology based on central composite design. The estimated optimal working conditions were as follows: [H2O2] &lt;0.132 M, GAC dosage = 108–123 g/L, and initial [IPA] = 0.038–0.10 M. The findings indicated that the dosage of GAC and the initial IPA concentration strongly affected the overall IPA removal. The values of R2 = 0.9948 and adjusted R2 = 0.9901 demonstrated that the response variability could be explained by the model expressing a satisfactory quadratic fit. Finally, the H2O2/GAC/MW process showed a faster and higher IPA removal rate than other processes tested.

Highly active and stable protease production by an extreme halophilic archaeon Haloarcula sp. TG1 isolated from Lake Tuz, Turkey

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Büşra Abanoz ◽  
Sezer Okay ◽  
Aslıhan Kurt-Kızıldoğan
Keyword(s):  
Protease Activity ◽  
Protease Production ◽  
Extreme Conditions ◽  
Salt Mine ◽  
Producer Strain ◽  
Extreme Halophiles ◽  
Highly Active ◽  
Protease Enzyme ◽  
Wide Range

AbstractObjective:Isolation of halophilic microorganisms from Çankırı salt mine and Lake Tuz in Turkey to explore versatile protease producers for industry and characterization of protease enzyme from the best protease producer among the isolated strains.Methods:Extreme halophiles were isolated from salt samples of Çankırı salt mine and Lake Tuz. Their protease activities were determined. The isolate with the highest protease activity was characterized. Its protease activity was evaluated in different NaCl concentrations, temperature and pH ranges, and in the presence of different inhibitors and metals. Thermostability and pH stability were also determined.Results:The highest protease producer strain was identified asConclusion:Due to the high activity and stability over a wide range of extreme conditions,

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