Detection ofToxoplasma gondiiby PCR and Mouse Bioassay in Rodents of Ahvaz District, Southwestern Iran
Toxoplasma gondiiis obligate coccidian zoonotic parasite. Felidae family is definitive and wide ranges of warm-blooded vertebrates are intermediate hosts for the parasite. Rodents are measured as an important source ofT. gondiiinfection for the definitive host. Thus, this study aimed to investigateToxoplasminfection in rodents of Ahvaz district, southwest of Iran. A total of 100 rodents (73Rattus norvegicus, 21Rattus rattus, and 6Mus musculus) were collected and studied by GRA6PCR and mouse bioassay. The finding indicated that 6 out of 100 (6%) and 2 out of 100 (2%) samples were positive by PCR and mouse bioassay, respectively. The results show notable chronic infection in the rodent and potential transmission of the infection among animal and men in the region. Accordingly, this study recommended investigating of theT. gondiiinfection in definitive and other intermediate hosts in other points of Khuzestan province, Southwest, Iran.