scholarly journals Detection ofToxoplasma gondiiby PCR and Mouse Bioassay in Rodents of Ahvaz District, Southwestern Iran

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
J. Saki ◽  
S. Khademvatan

Toxoplasma gondiiis obligate coccidian zoonotic parasite. Felidae family is definitive and wide ranges of warm-blooded vertebrates are intermediate hosts for the parasite. Rodents are measured as an important source ofT. gondiiinfection for the definitive host. Thus, this study aimed to investigateToxoplasminfection in rodents of Ahvaz district, southwest of Iran. A total of 100 rodents (73Rattus norvegicus, 21Rattus rattus, and 6Mus musculus) were collected and studied by GRA6PCR and mouse bioassay. The finding indicated that 6 out of 100 (6%) and 2 out of 100 (2%) samples were positive by PCR and mouse bioassay, respectively. The results show notable chronic infection in the rodent and potential transmission of the infection among animal and men in the region. Accordingly, this study recommended investigating of theT. gondiiinfection in definitive and other intermediate hosts in other points of Khuzestan province, Southwest, Iran.

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Shokrollah Salmanzadeh ◽  
Masoud Foroutan-Rad ◽  
Shahram Khademvatan ◽  
Sasan Moogahi ◽  
Shahla Bigdeli

Iran is considered as one of the malaria endemic countries of the Eastern Mediterranean Region (EMR) and is at risk due to neighboring Afghanistan, Pakistan in the east, and Iraq to the west. Therefore the aim of the present investigation is the evaluation of the trend of malaria distribution during the past decade (2001–2014) in Khuzestan province, southwestern Iran. In this retrospective cross-sectional investigation, blood samples were taken from all malaria suspicious cases who were referred to health centers across Khuzestan province. For each positive subject a questionnaire containing demographic information was filled out. Data analysis was performed using SPSS 18. From a total of 541 malaria confirmed cases, 498 (92.05%) were male and 43 (7.95%) were female. The highest number of infections was seen in 2001 with 161 (29.75%) cases and the lowest was in 2014 with 0 (0%). Also,Plasmodium vivaxwas identified as dominant species in 478 (88.35%) individuals andP. falciparumcomprised 63 (11.65%). The highest infection rate was observed in non-Iranian populations with number 459 (84.85%) and imported cases 508 (93.90%). Also, the majority of subjects were over 15 years of age, 458 (84.65%). Due to proximity to endemic countries which has made the malaria campaign difficult, more effort is needed to control the infection in order to achieve malaria elimination.


2021 ◽  
Vol 16 (3) ◽  
Author(s):  
Mehri Kargar ◽  
Homa Hajjaran ◽  
Javad Moazen ◽  
Mohammad Reza Shirzadi ◽  
Elham Kazemirad ◽  
...  

Background: Dezful and its suburbs, as the second city in Khuzestan Province, southwest of Iran, has been an endemic area of cutaneous leishmaniasis (CL) with a low incidence rate since the last decades. However, the disease incidence has rapidly increased, and now is considered as a re-emerging parasitic disease in the area. Objectives: The aim of this study was to identify the most prevalent CL species in Dezful Region. Methods: A total of 196 microscopically confirmed slides from CL patients referred to Dezful Health Center were randomly collected in the period of 2015 - 2016. After DNA extraction from microscopically positive slides, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out on 61 eligible specimens using ribosomal internal transcribed spacer 1 (ITS1) gene. The HaeIII restriction enzyme was used for the identification of species. Results: Samples were randomly collected from 196 acute CL cases, including 110 (56.2%) males and 86 (43.8%) females. Most infections were seen in the age range of 2 - 7 years (65/196, 33.1%). Totally, 60.1% of the cases had only one lesion, and half of the lesions appeared on hands. Furthermore, 162 (82.7%) cases were referred to Dezful Health Center in the cold seasons (autumn and winter). Results of PCR-RFLP on 61 eligible isolates showed that 60 (98.4%) isolates were Leishmania major, and only 1 (1.6%) isolate was Leishmania tropica. Conclusions: Our findings indicated that L. major is the main agent of re-emerged CL in Dezful and its suburbs, and the disease is a zoonosis.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sanam Hariri ◽  
Zahra Rahimi ◽  
Nahid Hashemi-Madani ◽  
Seyyed Ali Mard ◽  
Farnaz Hashemi ◽  
...  

Background The Middle East and North Africa (MENA) is postulated to have the highest increase in the prevalence of diabetes by 2030; however, studies on the epidemiology of diabetes are rather limited across the region, including in Iran. Methods This study was conducted between 2016 and 2018 among Iranian adults aged 20 to 65 years residing in Khuzestan province, southwestern Iran. Diabetes was defined as the fasting blood glucose (FBG) level of 126 mg/dl or higher, and/or taking antidiabetic medications, and/or self-declared diabetes. Prediabetes was defined as FBG 100 to 125 mg/dl. Multinomial logistic regression models were used to examine the association of multiple risk factors that attained significance on the outcome. Results Overall, 30,498 participants were recruited; the mean (±SD) age was 41.6 (±11.9) years. The prevalence of prediabetes and diabetes were 30.8 and 15.3%, respectively. We found a similar prevalence of diabetes in both sexes, although it was higher among illiterates, urban residents, married people, and smokers. Participants aged 50–65 and those with Body Mass Index (BMI) 30 kg/m2 or higher were more likely to be affected by diabetes [RR: 20.5 (18.1,23.3) and 3.2 (3.0,3.6)]. Hypertension [RR: 5.1 (4.7,5.5)], waist circumference (WC) equal or more than 90 cm [RR: 3.6 (3.3,3.9)], and family history [RR: 2.3 (2.2,2.5)] were also significantly associated with diabetes. For prediabetes, the main risk factors were age 50 to 65 years [RR: 2.6 (2.4,2.8)], BMI 30 kg/m2 or higher [RR: 1.9 (1.8,2.0)], hypertension and WC of 90 cm or higher [RR: 1.7 (1.6,1.8)]. The adjusted relative risks for all variables were higher in females than males, with the exception of family history for both conditions and waist circumference for prediabetes. Conclusions Prediabetes and diabetes are prevalent in southwestern Iran. The major determinants are older age, obesity, and the presence of hypertension. Further interventions are required to escalate diabetes prevention and diagnosis in high-risk areas across Iran.


2021 ◽  
Vol 44 (6) ◽  
pp. 42-48
Author(s):  
Dinka Grubišić ◽  
Ivan Juran ◽  
Mirjana Brmež ◽  
Maja Šurlog ◽  
Viktorija Sever ◽  
...  

Glodavci su važni štetnici uskladištenih poljoprivrednih proizvoda. Uz zadovoljenje osnovnih prehrambenih potreba, štetne vrste u skladištima nalaze povoljne uvjete za razvoj i razmnožavanje te zaštitu od prirodnih neprijatelja. Najštetnije vrste glodavaca u skladištima Republike Hrvatske jesu štakori vrsta Rattus norvegicus i Rattus rattus te domaći miš Mus musculus. Osim što se hrane uskladištenim proizvodima, rasipaju ih, onečišćuju urinom, izmetom, dlakom i slinom, navedene vrste rezervoari su zoonozama koje utječu na zdravlje ljudi, te domaćih i divljih životinja. U Republici Hrvatskoj u populacijama sitnih glodavaca dokazani su uzročnici Lajmske borelioza, trihineloze, leptospiroze, Q groznice i hemoragijske vrućice s bubrežnim sindromom. Utvrđene su i višestruke infekcije glodavaca uzročnicima zoonoza. Redovito praćenje brojnosti glodavaca važno je u svrhu pravovremenog suzbijanja te smanjenja materijalnih gubitaka, ali i u svrhu sprječavanja pojave epidemija zoonoza. Uz preventivne mjere koje podrazumijevaju održavanje higijene prostora te održavanje infrastrukture, čime sprječavamo privlačenje i nastanjivanje glodavaca u skladištima, provodi se i kontinuirana deratizacija koja uključuje primjenu mehaničkih, fizikalnih i kemijskih mjera zaštite.


2021 ◽  
Author(s):  
Seyed Yahya Mirzaee Aranki ◽  
Sorour Mazrae asl ◽  
Hosein Karimi Vardanjani

Abstract Changes in the quality of karstic water resources affected by the proximity to sources of salinity such as evaporate formations and oil brine pose major challenges to such water resources, especially in Khuzestan province, Iran. The karstic water resources of the Kamarderaz anticline plunge in Izeh city in Khuzestan province have hampered water supply management in the region. To determine water quality and the causes of water salinity in the study area, the hydrochemical parameters of the existing water resources were accordingly evaluated. To conduct hydrochemical studies, 21 samples were collected from different water sources of Kamarderaz anticline plunge in 2017–2018. To analyze the garnered data, Piper and Durov diagrams, composite graphs, ion exchange graphs, ion correlation diagrams, salinity source diagrams and ion ratios were used. The results showed that SO42−, Cl−, and Ca2+ in the water resources of the study area were likely due to several sources including carbonate minerals, gypsum dissolution, anhydrite, halite as well as oil brine. Besides, Br/Cl, I/Cl ion ratios as well as Br-Cl and SO4-Cl diagrams revealed the mixing of karstic freshwater with oil brine in the region. The result of TOC analysis also showed that hydrocarbon materials were present in the well of Atabaki (WT). Moreover, elemental sulfur and sulfur isotope analysis showed that the process of thermochemical sulfate reduction or bacterial sulfate reduction in the presence of hydrocarbons produced H2S gas, and eventually penetrated the WT and WH1.


2021 ◽  
Vol 15 (2) ◽  
pp. 44-51
Author(s):  
Ersandhi Resnhaleksmana ◽  
Mahardika Agus Wijayanti ◽  
Wayan Tunas Artama

Background: Cryptosporidium is a neglected zoonotic disease, but with the , its incidence is increasing. Animals such as rats and pigs can act as intermediate hosts and transmit Cryptosporidium to humans due to their proximity. Transmission occurs due to the ability of Cryptosporidium to survive . The research aimed to identify and describe the transmission of Cryptosporidium from animals to humans. Materials and Methods: This research was a cross sectional study and samples were collected from caught in residential areas, 205 pigs, and 438 humans in West Lombok. Fecal samples were examined using polymerase chain reaction (PCR) and sequencing to isolate the presence of Cryptosporidium, and identify the genetic similarity of the parasites found in rats and pigs with those that infect humans. Results: The PCR results found Cryptosporidium parvum in 4.76% (4/84) in rats; 6.34% 13/205) in pigs; and 0.91% (4/438) in humans. The sequencing results showed genetic kinship of C. parvum in rats, pigs, and humans. Conclusion: There are genetic similarities of Cryptosporidium in animals and humans, requiring that the Public Health programs in those contaminated areas must receive priority attention to prevent further transmission of these potentially fatal parasites.


2002 ◽  
Vol 55 (3) ◽  
pp. 55-3 ◽  
Author(s):  
Stefan Rothenburg ◽  
Maike Eiben ◽  
Friedrich Koch-Nolte ◽  
Friedrich Haag

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