scholarly journals Differences in Extended-Spectrum Beta-Lactamase ProducingEscherichia coliVirulence Factor Genes in the Baltic Sea Region

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Jana Lillo ◽  
Kristiine Pai ◽  
Arta Balode ◽  
Mariia Makarova ◽  
Kristi Huik ◽  
...  

The aim of this study was to compare the prevalence of different virulence factor (VF) genes in extended-spectrum beta-lactamase (ESBL) producingEscherichia colistrains isolated from the Baltic Sea region. A total of 432 strains of phenotypically ESBL positiveE. coliwere collected from 20 institutions located in Estonia, Latvia, Lithuania, and the region of St. Petersburg in Russia from January to May 2012 and analyzed for phylogenetic group and prevalence of 23 VF genes. The strains were collected from clinical material (urine, blood, wound, and respiratory tract). Bacterial isolates were compared according to phylogenetic group, clinical material, and geographical origin. Most of the VF genes were concentrated within phylogenetic group B2 and/or D. When comparing strains isolated from different countries, it was found that strains originating from Estonia and Latvia belonged mainly to group B2 and strains from Lithuania and Russia mainly to groups B2 and D. The P-fimbrial adhesin genepapEFwas more prevalent in Russian strains, colicin genecvaCin Lithuanian strains, and capsular genekpsMTIIin Latvian strains; serum resistant genetraTwas less prevalent in Estonian strains. The regional differences of VF genes remained statistically significant after taking into account the phylogenetic distribution in the countries.

2010 ◽  
Vol 76 (24) ◽  
pp. 8126-8134 ◽  
Author(s):  
Ivan Literak ◽  
Monika Dolejska ◽  
Dagmar Janoszowska ◽  
Jolana Hrusakova ◽  
Wlodzimierz Meissner ◽  
...  

ABSTRACT Individual cloacal swabs of mallards (Anas platyrhynchos) and of herring gulls (Larus argentatus), as well as samples of waterbird feces obtained in 2008 and 2009, were cultivated for Escherichia coli. Isolates of E. coli were tested for susceptibilities to 12 antimicrobial agents by the disk diffusion method. Moreover, the samples were subcultivated on MacConkey agar (MCA) containing cefotaxime (2 mg liter−1) to detect E. coli with extended-spectrum beta-lactamase (ESBL) and subsequently on MCA supplemented with ciprofloxacin (0.05 mg liter−1) and MCA with nalidixic acid (20 mg liter−1) to isolate fluoroquinolone-resistant E. coli. PCR was used to detect specific antibiotic resistance genes. We found 9 E. coli isolates producing ESBL with bla genes: bla CTX-M-1 (6 isolates), bla CTX-M-9 plus bla TEM-1b (1 isolate), bla CTX-M-15 plus bla OXA-1 (1 isolate), and bla SHV-12 (1 isolate). In the isolate with bla CTX-M-15, the gene aac(6)-Ib-cr was also detected. The bla genes were harbored by transferable plasmids of the IncN and IncI1 groups. Nine quinolone-resistant E. coli isolates with qnrS genes were found and characterized. The gene qnrS was associated with a Tn3-like transposon on the IncX1 plasmid together with bla TEM-1 in two isolates. The gene qnrS was also harbored by conjugative plasmids of the IncN and IncX2 groups. Even if populations of wild birds are not directly influenced by antibiotic practice, we have demonstrated that antibiotic-resistant E. coli strains, including strains with various ESBL and qnrS genes, are found in the feces of wild birds on the coast of the Baltic Sea in Poland.


Author(s):  
Evgenia Salin ◽  
Jeremy Woodard ◽  
Krister Sundblad

AbstractGeological investigations of a part of the crystalline basement in the Baltic Sea have been performed on a drill core collected from the depth of 1092–1093 m beneath the Phanerozoic sedimentary cover offshore the Latvian/Lithuanian border. The sample was analyzed for geochemistry and dated with the SIMS U–Pb zircon method. Inherited zircon cores from this migmatized granodioritic orthogneiss have an age of 1854 ± 15 Ma. Its chemical composition and age are correlated with the oldest generation of granitoids of the Transscandinavian Igneous Belt (TIB), which occur along the southwestern margin of the Svecofennian Domain in the Fennoscandian Shield and beneath the Phanerozoic sedimentary cover on southern Gotland and in northwestern Lithuania. It is suggested that the southwestern border of the Svecofennian Domain is located at a short distance to the SW of the investigated drill site. The majority of the zircon population shows that migmatization occurred at 1812 ± 5 Ma, with possible evidence of disturbance during the Sveconorwegian orogeny.


Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 406
Author(s):  
Zuhura I. Kimera ◽  
Fauster X. Mgaya ◽  
Gerald Misinzo ◽  
Stephen E. Mshana ◽  
Nyambura Moremi ◽  
...  

We determined the phenotypic profile of multidrug-resistant (MDR) Escherichia coli isolated from 698 samples (390 and 308 from poultry and domestic pigs, respectively). In total, 562 Enterobacteria were isolated. About 80.5% of the isolates were E. coli. Occurrence of E. coli was significantly higher among domestic pigs (73.1%) than in poultry (60.5%) (p = 0.000). In both poultry and domestic pigs, E. coli isolates were highly resistant to tetracycline (63.5%), nalidixic acid (53.7%), ampicillin (52.3%), and trimethoprim/sulfamethoxazole (50.9%). About 51.6%, 65.3%, and 53.7% of E. coli were MDR, extended-spectrum beta lactamase-producing enterobacteriaceae (ESBL-PE), and quinolone-resistant, respectively. A total of 68% of the extended-spectrum beta lactamase (ESBL) producers were also resistant to quinolones. For all tested antibiotics, resistance was significantly higher in ESBL-producing and quinolone-resistant isolates than the non-ESBL producers and non-quinolone-resistant E. coli. Eight isolates were resistant to eight classes of antimicrobials. We compared phenotypic with genotypic results of 20 MDR E. coli isolates, ESBL producers, and quinolone-resistant strains and found 80% harbored blaCTX-M, 15% aac(6)-lb-cr, 10% qnrB, and 5% qepA. None harbored TEM, SHV, qnrA, qnrS, qnrC, or qnrD. The observed pattern and level of resistance render this portfolio of antibiotics ineffective for their intended use.


Author(s):  
Anneli Adler ◽  
Almir Karacic ◽  
Ann-Christin Rönnberg Wästljung ◽  
Ulf Johansson ◽  
Kaspars Liepins ◽  
...  

AbstractThe increased demand for wood to replace oil-based products with renewable products has lifted focus to the Baltic Sea region where the environment is favorable for woody biomass growth. The aim of this study was to estimate broad-sense heritabilities and genotype-by-environment (G×E) interactions in growth and phenology traits in six climatically different regions in Sweden and the Baltics. We tested the hypothesis that both bud burst and bud set have a significant effect on the early growth of selected poplar clones in Northern Europe. Provenance hybrids of Populus trichocarpa adapted to the Northern European climate were compared to reference clones with adaptation to the Central European climate. The volume index of stemwood was under low to medium genetic control with heritabilities from 0.22 to 0.75. Heritabilities for phenology traits varied between 0.31 and 0.91. Locally chosen elite clones were identified. G×E interactions were analyzed using pairwise comparisons of the trials. Three different breeding zones for poplars between the latitudes of 55° N and 60° N in the Baltic Sea Region were outlined. The studied provenance hybrids with origin from North America offer a great possibility to broaden the area with commercial poplar plantations in Northern Europe and further improve the collection of commercial clones to match local climates. We conclude that phenology is an important selection criterion after growth.


2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
A Liinamo ◽  
K Matinheikki-Kokko ◽  
I Gobina ◽  
A Villeruša

Abstract In the future, health promotion would require developed strategies that lead to stronger cross-sectoral cooperation. Cross-sectoral cooperation enables the integration of fragmented resources and competencies, which benefit service solutions for urban health. Healthy Boost “Urban Labs for Better Health for All in the Baltic Sea Region”, funded by the EU Interreg Baltic Sea Region -program, aims to develop the Model for cross-sectoral cooperation, which will be tested in the cities of the Baltic Sea Region during 2020-21. The self-assessment tool for cross-sectoral cooperation was developed, and the self-assessment among the nine cities in seven countries from the Baltic Sea Region was conducted in 2019. The results indicated to what extent the staff (n = 329) in the cities have recognized the cross-sectoral cooperation for health and wellbeing as strategically crucial in their policies, communication, and in the design of their organizational functions. The daily practices were evaluated in terms of how systematically cities have implemented cross-sector actions for health and wellbeing. The biggest challenges for cooperating across sectors for the cities were coordination and systematic identification of the community needs for health promotion. The cooperative actions were less systematic than expected in the strategic approach. The variation among respondents' assessments was high within the cities that lead to a conclusion about existing gaps in coordination, communication, and leadership of cross-sectoral work within the cities. The Likert type self-assessment measurement was statistically reliable in both strategic and operational dimensions of cooperation. Key messages Evaluation and measurements are needed to identify cross-sectoral actions to health and well-being. The evidence-based Model developed in the Healthy Boost project will guide partners towards systematic cross-sectoral cooperation processes.


2013 ◽  
Vol 19 (1-2) ◽  
pp. 47-62 ◽  
Author(s):  
Petri Tapio ◽  
Vilja Varho ◽  
Hanna Heino

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