scholarly journals Optimization of Processing Parameters for Extraction of Amylase Enzyme from Dragon (Hylocereus polyrhizus) Peel Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Mehrnoush Amid ◽  
Mohd Yazid Abdul Manap ◽  
Norkhanani Zohdi
Keyword(s):  
Storage Stability ◽  
Specific Activity ◽  
Mixing Time ◽  
Temperature Stability ◽  
Processing Parameters ◽  
Ph Stability ◽  
Extraction Condition ◽  
Main Effect ◽  
And Storage ◽  
Optimum Extraction

The main goal of this study was to investigate the effect of extraction conditions on the enzymatic properties of thermoacidic amylase enzyme derived from dragon peel. The studied extraction variables were the buffer-to-sample (B/S) ratio (1 : 2 to 1 : 6, w/w), temperature (−18°C to 25°), mixing time (60 to 180 seconds), and the pH of the buffer (2.0 to 8.0). The results indicate that the enzyme extraction conditions exhibited the least significant (P<0.05) effect on temperature stability. Conversely, the extraction conditions had the most significant (P<0.05) effect on the specific activity and pH stability. The results also reveal that the main effect of the B/S ratio, followed by its interaction with the pH of the buffer, was significant (P<0.05) among most of the response variables studied. The optimum extraction condition caused the amylase to achieve high enzyme activity (648.4 U), specific activity (14.2 U/mg), temperature stability (88.4%), pH stability (85.2%), surfactant agent stability (87.2%), and storage stability (90.3%).

scholarly journals Multiscale Study on the Effect of Nano-Organic Montmorillonite on the Performance of Rubber Asphalt

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Xiaoge Tian ◽  
Ren Zhang ◽  
Zhen Yang ◽  
Yantian Chu ◽  
Yichao Xu ◽  
...  
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Storage Stability ◽  
Temperature Stability ◽  
High Temperature Stability ◽  
Scanning Calorimetry ◽  
Microscopic Mechanism ◽  
Organic Montmorillonite ◽  
Temperature Performance ◽  
And Storage

In order to improve the high-temperature performance, antiaging performance, and storage stability of rubber asphalt, nano-organic montmorillonite (NOMMT) was mixed with rubber asphalt. Macroscopic influences of NOMMT on rubber asphalt were measured through penetration, softening point, ductility, rotational viscosity tests, dynamic shear rheology test, and bending beam rheology test at low temperature and were conducted on rubber asphalt with different contents of NOMMT. Then, the microscopic mechanism of NOMMT on the microscopic performance of rubber asphalt was studied through using scanning electron microscopy (SEM), infrared spectroscopy (IR), and differential scanning calorimetry (DSC). The results showed that the rubber particles were smoother, uniform, and dispersed after NOMMT was introduced, and the compatibility between NOMMT and crumbed rubber powder was good. Some stable structures were formed in the composite modified asphalt. The disappearance of alcohol phenol and the increase in related groups such as alkane, benzene, and hydrocarbon indicated that chemical reaction occurred between NOMMT and rubber asphalt, resulting in the changes of the performance of the composite modified system, so that high-temperature stability, antiaging properties, and storage stability were improved but its low-temperature performance was decreased.

scholarly journals Kinetic Characterization and Effect of Immobilized Thermostable β-Glucosidase in Alginate Gel Beads on Sugarcane Juice

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Keerti ◽  
Anuradha Gupta ◽  
Vinod Kumar ◽  
Ashutosh Dubey ◽  
A. K. Verma
Keyword(s):  
Storage Stability ◽  
Immobilized Enzyme ◽  
Residual Activity ◽  
Sugarcane Juice ◽  
Ph Stability ◽  
Gel Beads ◽  
Optimum Ph ◽  
Enzyme Change ◽  
Ph Range ◽  
And Storage

A thermostable β-glucosidase was effectively immobilized on alginate by the method of gel entrapment. After optimization of immobilized conditions, recovered enzyme activity was 60%. Optimum pH, temperature, kinetic parameters, thermal and pH stability, reusability, and storage stability were investigated. The Km and Vmax for immobilized β-glucosidase were estimated to be 5.0 mM and 0.64 U/ml, respectively. When comparing, free and immobilized enzyme, change was observed in optimum pH and temperature from 5.0 to 6.0 and 60°C to 80°C, respectively. Immobilized enzyme showed an increase in pH stability over the studied pH range (3.0–10.0) and stability at temperature up to 80°C. The storage stability and reusability of the immobilized β-glucosidase were improved significantly, with 12.09% activity retention at 30°C after being stored for 25 d and 17.85% residual activity after being repeatedly used for 4 times. The effect of both free and immobilized β-glucosidase enzyme on physicochemical properties of sugarcane juice was also analyzed.

scholarly journals Asphalt Incorporation with Ethylene Vinyl Acetate (EVA) Copolymer and Natural Rubber (NR) Thermoplastic Vulcanizates (TPVs): Effects of TPV Gel Content on Physical and Rheological Properties

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1397
Author(s):  
Nappaphan Kunanusont ◽  
Boonchai Sangpetngam ◽  
Anongnat Somwangthanaroj
Keyword(s):  
Storage Stability ◽  
Ethylene Vinyl Acetate ◽  
Softening Temperature ◽  
Plastic Waste ◽  
Gel Content ◽  
Modified Asphalt ◽  
C Storage ◽  
Thermoplastic Vulcanizates ◽  
Polymer Modified Asphalt ◽  
And Storage

Plastic waste has been incorporated with asphalt to improve the physical properties of asphalt and alleviate the increasing trend of plastic waste being introduced into the environment. However, plastic waste comes in different types such as thermoplastic or thermoset, which results in varied properties of polymer modified asphalt (PMA). In this work, four thermoplastic vulcanizates (TPVs) were prepared using different peroxide concentrations to produce four formulations of gel content (with varying extent of crosslinked part) in order to imitate the variation of plastic waste. All four TPVs were then mixed with asphalt at 5 wt% thus producing four formulations of PMA, which went through physical, rheological, and storage stability assessments. PMA with higher gel content possessed lower penetration and higher softening temperature, indicating physically harder appearance of PMA. Superpave parameters remained unchanged among different gel content PMA at temperatures of 64, 70, and 76 °C. PMA with any level of gel content had lower Brookfield viscosity than PMA without gel content at a temperature of 135 °C. Higher gel content resulted in shorter storage stability measured with greater different softening temperatures between top and bottom layers of PMA after 5 days of 163 °C storage. This study shows that asphalt with thermoset plastic waste is harder and easier to pave, thus making the non-recycling thermoset plastic waste more useful and friendly to the environment.

Effect of extrusion on physical and chemical properties and storage stability of corn germ

2021 ◽  
Author(s):  
Zhigang Xiao ◽  
Kexin Wang ◽  
Fanshu Li ◽  
Huiying Cao ◽  
Qingsong Duan ◽  
...  
Keyword(s):  
Storage Stability ◽  
Chemical Properties ◽  
Corn Germ ◽  
Physical And Chemical Properties ◽  
Physical And Chemical ◽  
And Storage ◽  
And Chemical Properties

scholarly journals Folate in Red Rhapsody Strawberry—Content and Storage Stability.

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 47
Author(s):  
Julius Rami ◽  
Caroline Dumler ◽  
Nadine Weber ◽  
Michael Rychlik ◽  
Gabriele Netzel ◽  
...  
Keyword(s):  
Storage Stability ◽  
Active Form ◽  
Food Composition ◽  
Biologically Active ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
C Storage ◽  
Vitamin B9 ◽  
Adults And Children ◽  
And Storage

Folate (Vitamin B9) is critical for a range of biological functions in adults and children, including DNA, protein and neurotransmitter synthesis. It is also essential for the healthy development of the fetus in early pregnancy and for the prevention of neural tube defects, such as spina bifida. Strawberries are considered a tasty and healthy fruit consumed all over the world and may potentially be an important dietary source of natural folates. However, the relative importance of strawberry as a dietary source will depend on the total folate concentration, vitamer profile, storage stability and bioavailability to humans. Red Rhapsody, an important commercial strawberry cultivar in Australia, was screened for its folate content and storage stability by stable isotope dilution assay (SIDA). Total folate content ranged from 90–118 μg/100 g fresh weight (fw), which was well above the value in the Australian Food Composition Database (39 μg/100 g fw). 5-Methyltetrahydrofolate, the biologically active form in humans, was the principal vitamer present. Furthermore, folate remained relatively stable during refrigerated (4 °C) storage (loss of only 28% after 14 days of storage). This information is relevant for consumers since the inherent perishability of strawberry fruit makes refrigerated storage common practice in Australian households.

scholarly journals Characterization and storage stability of spray dried soy-rapeseed lecithin/trehalose liposomes loaded with a tilapia viscera hydrolysate

2021 ◽  
pp. 102708
Author(s):  
Cindy T. Sepúlveda ◽  
Ailén Alemán ◽  
José E. Zapata ◽  
M. Pilar Montero ◽  
M. Carmen Gómez-Guillén
Keyword(s):  
Storage Stability ◽  
And Storage ◽  
Spray Dried ◽  
Rapeseed Lecithin

Effect of heat-treated flour on the quality and storage stability of fresh noodles

LWT ◽  
2021 ◽  
pp. 111463
Author(s):  
Tingting Hong ◽  
Qiyan Zhao ◽  
Dan Xu ◽  
Yirong Yuan ◽  
Yue Ma ◽  
...  
Keyword(s):  
Storage Stability ◽  
Heat Treated ◽  
And Storage
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