scholarly journals Piper nigrumLeaf and Stem Assisted Green Synthesis of Silver Nanoparticles and Evaluation of Its Antibacterial Activity Against Agricultural Plant Pathogens

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kanniah Paulkumar ◽  
Gnanadhas Gnanajobitha ◽  
Mahendran Vanaja ◽  
Shanmugam Rajeshkumar ◽  
Chelladurai Malarkodi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Electron Microscope ◽  
Plant Pathogens ◽  
Xrd Analysis ◽  
Piper Nigrum ◽  
X Ray ◽  
Agricultural Plant ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract ofPiper nigrum. The synthesized nanoparticle was characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), energy dispersive X-ray analysis (EDAX), and Fourier Transform Infrared Spectroscopy (FTIR). The observation of the peak at 460 nm in the UV-vis spectra for leaf- and stem-synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. Further, XRD analysis has been carried out to confirm the crystalline nature of the synthesized silver nanoparticles. The TEM images show that the leaf- and stem-synthesized silver nanoparticles were within the size of about 7–50 nm and 9–30 nm, respectively. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. Further, the antibacterial activity of the green-synthesized silver nanoparticles was examined against agricultural plant pathogens. The antibacterial property of silver nanoparticles is a beneficial application in the field of agricultural nanotechnology.

scholarly journals Herbal Plant Synthesis of Antibacterial Silver Nanoparticles by Solanum trilobatum and Its Characterization

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Vanaja ◽  
K. Paulkumar ◽  
G. Gnanajobitha ◽  
S. Rajeshkumar ◽  
C. Malarkodi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Electron Microscope ◽  
Green Synthesis ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Solanum Trilobatum ◽  
Green Synthesized Silver Nanoparticles

Green synthesis method of nanomaterials is rapidly growing in the nanotechnology field; it replaces the use of toxic chemicals and time consumption. In this present investigation we report the green synthesis of silver nanoparticles (AgNPs) by using the leaf extract of medicinally valuable plant Solanum trilobatum. The influence of physical and chemical parameters on the silver nanoparticle fabrication such as incubation time, silver nitrate concentration, pH, and temperature is also studied in this present context. The green synthesized silver nanoparticles were characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), energy dispersive X-ray (EDX), and transmission electron microscope (TEM). The SEM and TEM confirm the synthesis of spherical shape of nanocrystalline particles with the size range of 2–10 nm. FTIR reveals that the carboxyl and amine groups may be involved in the reduction of silver ions to silver nanoparticles. Antibacterial activity of synthesized silver nanoparticles was done by agar well diffusion method against different pathogenic bacteria. The green synthesized silver nanoparticles can be used in the field of medicine, due to their high antibacterial activity.

Ultra-sonication enhanced green synthesis of silver nanoparticles using Barleria buxifolia leaf extract and its possible application

2021 ◽  
Author(s):  
Vanaraj sekar
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Cancer Cell Line ◽  
Xrd Analysis ◽  
Blue Dye ◽  
Multiple Use ◽  
Uniform Size ◽  
X Ray ◽  
Vis Spectroscopy

Abstract A simple and eco-friendly method for the green synthesis of silver nanoparticles (AgNPs) by ultrasound-assisted strategy using Barleria buxifolia leaf extract as a reducing and capping agent was established in this study. The obtained AgNPs were characterized. UV-vis spectrum, Fourier transform infrared spectroscopy (FTIR), scanning and transmission electron microscopy (SEM and TEM), Energy Dispersive X-Ray Analyzer (EDX), X-ray diffraction, dynamic light scattering (DLS) analysis showed that the obtained AgNPs were mono dispersed spheres with uniform size of 80 nm. UV-vis spectroscopy, FTIR, and XRD analysis indicated that the surface of the obtained AgNPs was covered with organic molecules in plant extracts. The results of ABTS assays showed that high antioxidant activity was seen in the obtained AgNPs. Green synthesized AgNPs showed potent antibacterial and anti-biofilm activity against tested pathogens. Cytotoxicity assay showed that the obtained AgNPs were significantly cytotoxic to cancer cell line (MCF-7). In addition, the AgNPs synthesized in this paper can also photo catalytically degrade methylene blue dye under visible light. The potent bioactivity exhibited by the green synthesized silver nanoparticles leads towards the multiple use as antioxidant, antibacterial, anti-biofilm, cytotoxic as well as photo catalytic agent.

scholarly journals Green synthesis of Ag/TiO2 Nanocomposite Assisted by Gambier Leaf (Uncaria gambir Roxb) Extract

2019 ◽  
Vol 22 (6) ◽  
pp. 250-255 ◽  
Author(s):  
Sry Wahyuni ◽  
Syukri Syukri ◽  
Syukri Arief
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Diffuse Reflectance Spectroscopy ◽  
Irradiation Time ◽  
Rutile Phase ◽  
X Ray Diffraction ◽  
Silver Surface ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Studies of green synthesis of nanoparticles mediated by plants extract is extensively explored and studied in recent time due to eco-friendliness, cost-effectiveness, and use a minimum amount of toxic chemicals in the process of inorganic material synthesis. In this study, the immobilization of silver nanoparticles on the surface of titanium dioxide (TiO2) was carried out using Uncaria gambier Roxb. leaf extract as a silver ion (Ag+) reducing agent. The synthesized Ag/TiO2 nanocomposite was characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), and Diffuse Reflectance Spectroscopy (DRS). The formation of silver nanoparticles was confirmed through UV-Vis spectroscopic analysis, which showed a silver surface plasmon resonance (SPR) band at 426 nm. The X-ray diffraction pattern shows that Ag can inhibit the transition of the anatase into rutile phase. The presence of Ag particles in TiO2 can increase the absorption ability from an initial wavelength of 407 nm to 438 nm. Based on the results of Rhodamin B degradation, it can be seen that Ag/TiO2 has a higher photocatalytic activity than bare TiO2 with 99% percent degradation at 120 minutes of irradiation time.

scholarly journals Green synthesis and antibacterial activity studies of silver nanoparticles from the aqueous extracts of Thespesia populnea

2020 ◽  
Vol 9 (1) ◽  
pp. 931-934
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Inhibitory Concentration ◽  
Xrd Analysis ◽  
Leaf Extracts ◽  
Swarming Motility ◽  
Thespesia Populnea ◽  
Vis Spectroscopy ◽  
Test Organisms

Green synthesis of silver nanoparticles were carried out using the aqueous leaf extracts of Thespesia populnea and characterised by UV Vis spectroscopy, SEM, EDX, AFM and XRD analysis. The silver nanoparticles when tested on Pseudomonas aeruginosa and Bacillus subtilis, was found to have potent antibacterial activity. Further, the microbes were susceptible for a minimum inhibitory concentration of 0.5 µg and also arrested the swarming motility of the test organisms. The nanoparticles interacted with the microbial membrane which led to the leakage of proteins from the cells.

scholarly journals PHYTOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM RUELLIA TUBEROSA (L.): EFFECT OF PHYSICOCHEMICAL PARAMETERS

2021 ◽  
pp. 31-38
Author(s):  
HARIKA M ◽  
RADHIKA P
Keyword(s):  
Silver Nanoparticles ◽  
Metal Ion ◽  
Incubation Temperature ◽  
Ion Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Face Centered Cubic ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy ◽  
Tuber Extract

Objective: The current study focused on synthesizing silver nanoparticles (AgNPs) using Ruellia tuberosa aqueous tuber extract (RTTE) and silver nitrate (AgNO3) solution. Methods: AgNPs were synthesized using an aqueous tuber extract of the medicinal herb R. tuberosa (L.). The existence of significant phytoconstituents involved in synthesizing the AgNPs was determined using the gas chromatography–mass spectrometry (GC–MS) study. We evaluated the physical and chemical parameters such as the effect of time, temperature, metal ion concentration, crude aqueous tuber extract concentration, and pH in the synthesis of nanoparticles. The AgNPs were characterized using ultraviolet (UV)–Vis spectroscopy, field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), high-resolution transmission electron microscope (HRTEM), selected area electron diffraction (SAED), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) techniques. Results: R. tuberosa tuber extract was rich in various phytochemical constituents which were identified by GC–MS. For biosynthesis, the optimal values were 1 mM AgNO3concentration, 0.1 mL of aqueous tuber extract, and a 40 min incubation temperature of 70°C. The existence of a characteristic surface plasmon resonance (SPR) peak at 421 nm indicated the biosynthesis of AgNPs using UV–Vis spectroscopy. At higher temperatures and alkaline pH, the development of AgNPs increased overtime and remained stable up to 4 weeks. FESEM, EDX, HRTEM, SAED, and XRD analysis revealed that most AgNPs were spherical, with an average size distribution of 34.9 nm and a crystalline phase, face-centered cubic lattice. Infrared (FTIR) spectroscopic analysis revealed that hydroxyl and amino functional groups were involved in the biosynthesis and stabilization of AgNPs. Conclusion: The synthesis of AgNPs from R. tuberosa aqueous tuber extract was a cost-effective process and environmental friendly.

scholarly journals Biogenic Synthesis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Anju Arya ◽  
Khushbu Gupta ◽  
Tejpal Singh Chundawat ◽  
Dipti Vaya
Keyword(s):  
Silver Nanoparticles ◽  
Green Alga ◽  
Botryococcus Braunii ◽  
Biologically Active ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy ◽  
Active Nanoparticles

The spread of infectious diseases and the increase in the drug resistance among microbes has forced the researchers to synthesize biologically active nanoparticles. Improvement of the ecofriendly procedure for the synthesis of nanoparticles is growing day-by-day in the field of nanobiotechnology. In the present study, we use the extract of green alga Botryococcus braunii for the synthesis of copper and silver nanoparticles. The characterization of copper and silver nanoparticles was carried out by using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron spectroscopy (SEM). FTIR measurements showed all functional groups having control over reduction and stabilization of the nanoparticles. The X-ray diffraction pattern revealed that the particles were crystalline in nature with a face-centred cubic (FCC) geometry. SEM micrographs have shown the morphology of biogenically synthesized metal nanoparticles. Furthermore, these biosynthesized nanoparticles were found to be highly toxic against two Gram-negative bacterial strains Pseudomonas aeruginosa (MTCC 441) and Escherichia coli (MTCC 442), two Gram-positive bacterial strains Klebsiella pneumoniae (MTCC 109) and Staphylococcus aureus (MTCC 96), and a fungal strain Fusarium oxysporum (MTCC 2087). The zone of inhibition was measured by the agar well plate method, and furthermore, minimum inhibitory concentration was determined by the broth dilution assay.

scholarly journals Synthesis and Characterization of Silver Nanoparticles Using Alginate from the Brown Seaweed Laminaria ochroleuca: Structural Features and Antibacterial Activity

2021 ◽  
Vol 12 (5) ◽  
pp. 6046-6057
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Sodium Alginate ◽  
Brown Seaweed ◽  
Analytical Techniques ◽  
Spherical Shape ◽  
Structural Features ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Laminaria Ochroleuca

In this study, inexpensive and eco-friendly production of the silver nanoparticles (AgNPs) was explored using Laminaria ochroleuca sodium alginate as stabilizing and reducing agent. The synthesized nanoparticles were characterized by various analytical techniques such as UV–Vis spectroscopy, Energy dispersive X-ray spectrometer (EDX), X-ray diffraction (XRD), transmission electron microscopy (TEM), TEM selected area electron diffraction (SAED), Fourier transform infrared (FTIR) spectroscopy, and thermogravimetric analysis (TGA). The synthesis of AgNPs was elucidated by characteristic absorption peaks in the UV–Vis test. The FTIR analysis revealed the involvement of many functional groups in the bioreduction and the stabilization of AgNPs, while TEM images illustrated the spherical shape with maximum particles found in the size range of 10-20 nm. Prominent peaks and silver diffraction rings shown by the XRD spectrum and SAED pattern, respectively, confirmed the crystalline nature (fcc) of the synthesized AgNPs, which were found to be thermally stable based on TGA analysis. The AgNPs exhibited strong antibacterial activity against Gram+ and Gram– bacteria. The synthesized silver nanoparticles using Laminaria ochroleuca sodium alginate revealed interesting properties that could be potentially used for pharmaceutical applications.

scholarly journals Green Biosynthesis of Silver Nanoparticles Using Eriobotrya japonica (Thunb.) Leaf Extract for Reductive Catalysis

Materials ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 189 ◽  
Author(s):  
Chen Yu ◽  
Jingchun Tang ◽  
Xiaomei Liu ◽  
Xinwei Ren ◽  
Meinan Zhen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Leaf Extract ◽  
Reactive Dyes ◽  
Synthesis Temperature ◽  
Eriobotrya Japonica ◽  
X Ray ◽  
Green Approach ◽  
Selected Area Electron Diffraction ◽  
Vis Spectroscopy

This article reports on silver nanoparticles (AgNPs) that were green-synthesized by using Eriobotrya japonica (Thunb.) leaf extract and their use for the catalytic degradation of reactive dyes. The properties of biogenic AgNPs were characterized using UV-vis absorption spectroscopy, field emission scanning electron microscope (FESEM), X-ray powder diffraction (XRD), transmission electron microscope (TEM), Fourier transforming infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDS), and selected area electron diffraction (SAED) analysis. The UV-vis spectroscopy and X-ray analyses confirmed the formation of AgNPs and showed the strong absorbance around 467 nm with surface plasmon resonance (SPR). The mean diameter of biogenic AgNPs at room (20 °C), moderate (50 °C), and high temperatures (80 °C) were 9.26 ± 2.72, 13.09 ± 3.66, and 17.28 ± 5.78 nm, respectively. The reaction temperature had significant impacts on the sizes of synthesized AgNPs. The higher the synthesis temperature, the larger size and the lower catalysis activity for reductive decomposition of reactive dyes via NaBH4. The results supported a bio-green approach for developing AgNPs with a small size and stable degradation activity of reactive dyes over 92% in 30 min by using Eriobotrya japonica (Thunb.) leaf extract at pH 7, 20 °C, and 1:10 ratio of silver nitrate added to the leaf extract.

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

scholarly journals Optimization of Green Synthesis of Silver Nanoparticles from Leaf Extracts ofPimenta dioica(Allspice)

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akshay Rajeev Geetha ◽  
Elizabeth George ◽  
Akshay Srinivasan ◽  
Jameel Shaik
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Hot Air ◽  
Vis Spectroscopy ◽  
Pimenta Dioica ◽  
First Time

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.

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