scholarly journals The Differential Proteome of the ProbioticLactobacillus acidophilusNCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Twoβ-Glycoside Hydrolases

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Gabriella C. van Zanten ◽  
Nadja Sparding ◽  
Avishek Majumder ◽  
Sampo J. Lahtinen ◽  
Birte Svensson ◽  
...  
Keyword(s):  
Carbon Catabolite Repression ◽  
Molecular Level ◽  
Cell Extract ◽  
Probiotic Bacterium ◽  
Glycoside Hydrolases ◽  
Alkaline Ph ◽  
Late Exponential Phase ◽  
2D Dige ◽  
Difference Gel Electrophoresis ◽  
Insight Into

Probiotics, prebiotics, and combinations thereof, that is, synbiotics, are known to exert beneficial health effects in humans; however interactions between pro- and prebiotics remain poorly understood at the molecular level. The present study describes changes in abundance of different proteins of the probiotic bacteriumLactobacillus acidophilusNCFM (NCFM) when grown on the potential prebiotic cellobiose as compared to glucose. Cytosolic cell extract proteomes after harvest at late exponential phase of NCFM grown on cellobiose or glucose were analyzed by two dimensional difference gel electrophoresis (2D-DIGE) in the acidic (pH 4–7) and the alkaline (pH 6–11) regions showing a total of 136 spots to change in abundance. Proteins were identified by MS or MS/MS from 81 of these spots representing 49 unique proteins and either increasing 1.5–13.9-fold or decreasing 1.5–7.8-fold in relative abundance. Many of these proteins were associated with energy metabolism, including the cellobiose related glycoside hydrolases phospho-β-glucosidase (LBA0881) and phospho-β-galactosidase II (LBA0726). The data provide insight into the utilization of the candidate prebiotic cellobiose by the probiotic bacterium NCFM. Several of the upregulated or downregulated identified proteins associated with utilization of cellobiose indicate the presence of carbon catabolite repression and regulation of enzymes involved in carbohydrate metabolism.

scholarly journals Noni fruit’s water spot appearance on the second day of harvest: a trade-off between resistance and energy

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tian Wu ◽  
Danyan Hu ◽  
Qingfen Wang
Keyword(s):  
Statistical Analysis ◽  
Molecular Level ◽  
Morinda Citrifolia ◽  
Transcriptome Data ◽  
Trade Off ◽  
Kegg Pathways ◽  
Available Energy ◽  
Climacteric Fruit ◽  
The Relationship ◽  
Insight Into

Abstract Background Noni (Morinda citrifolia Linn.) is a tropical tree that bears climacteric fruit. Previous observations and research have shown that the second day (2 d) after harvest is the most important demarcation point when the fruit has the same appearance as the freshly picked fruit (0 d); however, they are beginning to become water spot appearance. We performed a conjoint analysis of metabolome and transcriptome data for noni fruit of 0 d and 2 d to reveal what happened to the fruit at the molecular level. Genes and metabolites were annotated to KEGG pathways and the co-annotated KEGG pathways were used as a statistical analysis. Results We found 25 pathways that were significantly altered at both metabolic and transcriptional levels, including a total of 285 differentially expressed genes (DEGs) and 11 differential metabolites through an integrative analysis of transcriptomics and metabolomics. The energy metabolism and pathways originating from phenylalanine were disturbed the most. The upregulated resistance metabolites and genes implied the increase of resistance and energy consumption in the postharvest noni fruit. Most genes involved in glycolysis were downregulated, further limiting the available energy. This lack of energy led noni fruit to water spot appearance, a prelude to softening. The metabolites and genes related to the resistance and energy interacted and restricted each other to keep noni fruit seemingly hard within two days after harvest, but actually the softening was already unstoppable. Conclusions This study provides a new insight into the relationship between the metabolites and genes of noni fruit, as well as a foundation for further clarification of the post-ripening mechanism in noni fruit.

scholarly journals Cisplatin under oriented external electric fields: A deeper insight into electrochemotherapy at the molecular level

2020 ◽  
Author(s):  
Li Zhang ◽  
Ya‐Ling Ye ◽  
Xiao‐Ling Zhang ◽  
Xiang‐Hui Li ◽  
Qiao‐Hong Chen ◽  
...  
Keyword(s):  
Electric Fields ◽  
Molecular Level ◽  
External Electric Fields ◽  
Insight Into

scholarly journals Molecular-Level Insight into How Hydroxyl Groups Boost Catalytic Activity in CO2 Hydrogenation into Methanol

Chem ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 613-625 ◽  
Author(s):  
Yuhan Peng ◽  
Liangbing Wang ◽  
Qiquan Luo ◽  
Yun Cao ◽  
Yizhou Dai ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Molecular Level ◽  
Co2 Hydrogenation ◽  
Hydroxyl Groups ◽  
Insight Into

scholarly journals Insight into molecular-level interactions between imidazolium-based ionic liquids and cellulose combining NMR, SAXS and MD simulations

2019 ◽  
Vol 75 (a1) ◽  
pp. a268-a268
Author(s):  
Aparna Annamraju ◽  
Nicholas D. Smith ◽  
Loukas Petridis ◽  
Hugh O'Neill ◽  
Sai Venkatesh Pingali ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Molecular Level ◽  
Md Simulations ◽  
Insight Into

Wingless induces transdetermination in developing Drosophila imaginal discs

Development ◽  
1995 ◽  
Vol 121 (5) ◽  
pp. 1263-1272 ◽  
Author(s):  
L. Maves ◽  
G. Schubiger
Keyword(s):  
Molecular Level ◽  
Ectopic Expression ◽  
Imaginal Discs ◽  
Developmental Studies ◽  
New Approach ◽  
Cell Determination ◽  
Disc Cells ◽  
The Subject ◽  
Insight Into

Drosophila imaginal discs, the precursors of the adult fly appendages, have been the subject of intensive developmental studies, particularly on cell determination. Cultured disc fragments are recognized not only for the ability to maintain their determined state through extra cell divisions but also for the ability to transdetermine, or switch to the determined state of a different disc. An understanding of transdetermination at a molecular level will provide further insight into the requirements for maintaining cell determination. We find that ectopic expression of the Drosophila gene wingless induces transdetermination of foreleg imaginal disc cells to wing cells. This transdetermination occurs in foreleg discs of developing larvae without disc fragmentation. The in situ-transdetermining cells localize to the dorsal region of the foreleg disc. This wingless-induced transdetermination event is remarkably similar to the leg-to-wing switch that occurs after leg disc culture. Thus we have identified a new approach to a molecular dissection of transdetermination.

scholarly journals Spectroscopic Studies of the Salmonella enterica Adenosyltransferase Enzyme SeCobA: Molecular-Level Insight into the Mechanism of Substrate Cob(II)alamin Activation

Biochemistry ◽  
2014 ◽  
Vol 53 (50) ◽  
pp. 7969-7982 ◽  
Author(s):  
Ivan G. Pallares ◽  
Theodore C. Moore ◽  
Jorge C. Escalante-Semerena ◽  
Thomas C. Brunold
Keyword(s):  
Salmonella Enterica ◽  
Molecular Level ◽  
Spectroscopic Studies ◽  
Insight Into

scholarly journals Molecular Level Insight into Enhanced n‐Type Transport in Solution‐Printed Hybrid Thermoelectrics

2019 ◽  
Vol 9 (13) ◽  
pp. 1803469 ◽  
Author(s):  
Edmond W. Zaia ◽  
Madeleine P. Gordon ◽  
Valerie Niemann ◽  
Jaeyoo Choi ◽  
Ruchira Chatterjee ◽  
...  
Keyword(s):  
Molecular Level ◽  
Insight Into

scholarly journals Revisiting the Metabolic Capabilities of Bifidobacterium longum susbp. longum and Bifidobacterium longum subsp. infantis from a Glycoside Hydrolase Perspective

2020 ◽  
Vol 8 (5) ◽  
pp. 723
Author(s):  
Guillermo Blanco ◽  
Lorena Ruiz ◽  
Hector Tamés ◽  
Patricia Ruas-Madiedo ◽  
Florentino Fdez-Riverola ◽  
...  
Keyword(s):  
In Silico ◽  
Genetic Background ◽  
Bifidobacterium Longum ◽  
In Silico Analysis ◽  
Glycoside Hydrolases ◽  
Computational Tools ◽  
Beneficial Effects ◽  
Silico Analysis ◽  
Insight Into

Bifidobacteria are among the most abundant microorganisms inhabiting the intestine of humans and many animals. Within the genus Bifidobacterium, several beneficial effects have been attributed to strains belonging to the subspecies Bifidobacterium longum subsp. longum and Bifidobacterium longum subsp. infantis, which are often found in infants and adults. The increasing numbers of sequenced genomes belonging to these two subspecies, and the availability of novel computational tools focused on predicting glycolytic abilities, with the aim of understanding the capabilities of degrading specific carbohydrates, allowed us to depict the potential glycoside hydrolases (GH) of these bacteria, with a focus on those GH profiles that differ in the two subspecies. We performed an in silico examination of 188 sequenced B. longum genomes and depicted the commonly present and strain-specific GHs and GH families among representatives of this species. Additionally, GH profiling, genome-based and 16S rRNA-based clustering analyses showed that the subspecies assignment of some strains does not properly match with their genetic background. Furthermore, the analysis of the potential GH component allowed the distinction of clear GH patterns. Some of the GH activities, and their link with the two subspecies under study, are further discussed. Overall, our in silico analysis poses some questions about the suitability of considering the GH activities of B. longum subsp. longum and B. longum subsp. infantis to gain insight into the characterization and classification of these two subspecies with probiotic interest.

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