scholarly journals Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract ofHovenia dulcis) on the Ovariectomized Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-17 ◽  
Author(s):  
Yun-Ho Hwang ◽  
Kwang-Jin Kim ◽  
Jong-Jin Kim ◽  
Kyung-Yun Kang ◽  
Sung-Ju Lee ◽  
...  
Keyword(s):  
Bone Loss ◽  
Cell Population ◽  
Immune Cell ◽  
Osteoclast Differentiation ◽  
Water Extract ◽  
Oriental Medicine ◽  
Trabecular Microarchitecture ◽  
Immune Cell Population ◽  
Ovariectomized Mice ◽  
Hovenia Dulcis

Protective effect of new oriental medicine (Kyungokgo mixed with water extract ofHovenia dulcis, KOGHD) was assessed on the bone loss induced mice by ovariectomy. In thein vivoexperiments, antiosteoporosis effect of KOGHD was investigated using ovariectomized osteoporosis mice model. After 6 weeks of treatment, the mice were euthanized, and the effect of Kyungokgo (KOG) and KOGHD on body weight, spleen weigh, thymus weight, uterine weight, serum biochemical indicators, bone weight and length, immune cell population, bone morphometric parameters, and histological stains was observed. Our results showed that KOGHD prevented the deterioration of trabecular microarchitecture caused by ovariectomy, which were accompanied by the lower levels of bone turnover markers and immune cell population as evidenced by the inhibition of RANKL-mediated osteoclast differentiation without cytotoxic effect on bone marrow derived macrophages (BMMs). Therefore, these results suggest that theHovenia dulcis(HD) supplementation in the KOG may also prevent and treat bone loss.

scholarly journals Forsythia suspensa Protects against Bone Loss in Ovariectomized Mice

Nutrients ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1831 ◽  
Author(s):  
Youn-Hwan Hwang ◽  
Seon-A Jang ◽  
Taesoo Kim ◽  
Hyunil Ha
Keyword(s):  
Bone Loss ◽  
Trabecular Bone ◽  
Osteoclast Differentiation ◽  
Water Extract ◽  
Nuclear Factor ◽  
Micro Computed Tomography ◽  
Activated T Cells ◽  
Beneficial Effects ◽  
Ovariectomized Mice ◽  
Forsythia Suspensa

In traditional oriental medicine, the fruit of Forsythia suspensa has been used as a nutritional supplement to alleviate inflammation and treat gastrointestinal diseases. However, there is no information available on its beneficial effects on bone. We investigated the beneficial effects of F. suspensa water extract (WFS) on osteoclast differentiation and bone loss. The microarchitecture of trabecular bone was analyzed by micro-computed tomography. Osteoclast differentiation was evaluated based on tartrate-resistant alkaline phosphatase activity, and bone resorption activity was examined on a bone-like mineral surface. The mechanism of action of WFS was assessed by evaluating the expression and activation of signaling molecules. Phytochemical constituents were identified and quantitated by ultrahigh-performance liquid chromatography–tandem mass spectrometry. WFS reduced ovariectomy-induced trabecular bone loss and inhibited receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation and resorption activity. WFS suppressed RANKL-induced expression of nuclear factor of activated T cells cytoplasmic 1, a crucial transcription factor for osteoclast differentiation by decreasing c-Fos protein levels and suppressing the activation of p38 and c-Jun-N-terminal kinase. We also identified 12 phytochemicals in WFS including lignans, phenylethanoids, and flavonoids. Collectively, these results suggest that WFS inhibits osteoclast differentiation and can potentially be used to treat postmenopausal osteoporosis.

scholarly journals Water Extract of Lysimachia christinae Inhibits Trabecular Bone Loss and Fat Accumulation in Ovariectomized Mice

Nutrients ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1927
Author(s):  
Ki-Shuk Shim ◽  
Youn-Hwan Hwang ◽  
Seon-A Jang ◽  
Taesoo Kim ◽  
Hyunil Ha
Keyword(s):  
Bone Loss ◽  
Trabecular Bone ◽  
Body Weight Gain ◽  
Osteoclast Differentiation ◽  
Water Extract ◽  
Mitogen Activated Protein Kinase ◽  
Nuclear Factor ◽  
Fat Accumulation ◽  
Ovariectomized Mice ◽  
Trabecular Bone Loss

In Asia, extracts of Lysimachia christinae have been used for liver or urinogenital system-related diseases in traditional medicine. In this study, we investigated the effects of the water extract of L. christinae (WELC) on receptor activator of nuclear factor-kappa Β ligand (RANKL)-induced osteoclastic differentiation of bone marrow macrophages, and on osteoporosis and obesity in ovariectomy mice. RANK signaling pathways related to osteoclast differentiation were examined by real-time polymerase chain reaction (PCR) and western blot analysis. Additionally, we performed micro-computed tomography to assess trabecular bone loss, histological analysis for fat accumulation in adipose, liver, and bone tissues, and phytochemical profiling for WELC characterization. WELC significantly inhibited osteoclast differentiation by downregulating RANKL-induced mitogen-activated protein kinase (MAPK)/c-Fos/nuclear factor of activated T-cells (NFAT) signaling in osteoclast precursors and ovariectomy-induced trabecular loss by suppressing osteolcastic bone resorption. WELC markedly decreased ovariectomy-induced body weight gain and fat accumulation in adipose, liver, and bone tissues. Furthermore, ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) identified 16 phytochemicals in WELC when compared with the mass fragmentation of standard chemicals. Collectively, these results suggest that WELC might possess beneficial effects on postmenopausal osteoporosis by inhibiting osteoclast differentiation and obesity by suppressing fat accumulation.

scholarly journals P062 Differences in immune cell population subsets in inflammatory bowel disease patients under anti-TNF treatment

2019 ◽  
Vol 13 (Supplement_1) ◽  
pp. S117-S117
Author(s):  
S Notararigo ◽  
J E Viñuela Roldán ◽  
M Abanades-Tercero ◽  
J E Dominguez-Munoz ◽  
M Barreiro-de Acosta
Keyword(s):  
Inflammatory Bowel Disease ◽  
Cell Population ◽  
Bowel Disease ◽  
Immune Cell ◽  
Immune Cell Population ◽  
Inflammatory Bowel

scholarly journals 110 Characterizing the immune cell population in the human fetal membrane

2021 ◽  
Vol 224 (2) ◽  
pp. S76-S77
Author(s):  
Sara Jacobs ◽  
Samantha Sheller-Miller ◽  
Lauren Richardson ◽  
Rheanna Urrabaz-Garza ◽  
Enkhtuya Radnaa ◽  
...  
Keyword(s):  
Cell Population ◽  
Immune Cell ◽  
Fetal Membrane ◽  
Immune Cell Population

scholarly journals Anti-Osteoporotic Effects of Commiphora Myrrha and Its Poly-Saccharide via Osteoclastogenesis Inhibition

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 945
Author(s):  
Youn-Hwan Hwang ◽  
Ami Lee ◽  
Taesoo Kim ◽  
Seon-A Jang ◽  
Hyunil Ha
Keyword(s):  
Bone Loss ◽  
Osteoclast Differentiation ◽  
Water Extract ◽  
Nuclear Factor Κb ◽  
Herbal Remedies ◽  
Specific Gene ◽  
Nuclear Factor ◽  
Activated T Cells ◽  
Herbal Products ◽  
Marrow Cavity

In traditional oriental medicines, Commiphora myrrha and its resinous exudate (i.e., myrrh) are used as herbal remedies to treat various inflammatory and metabolic disorders. Until now, C. myrrha-derived herbal products are considered useful source for bioactive compounds to manage numerous human diseases. This study investigated the effects of water extract of C. myrrha resin (WCM) and its polysaccharide (WCM-PE) on modulatory effects of osteoclast differentiation and/or ovariectomized-induced bone loss. Oral administration of WCM (200 and 500 mg/kg/day for four weeks) notably decreased trabecular bone loss and lipid accumulation in the bone marrow cavity. WCM and WCM-PE dose-dependently inhibited receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis and suppressed RANKL-mediated overexpression of c-Fos and nuclear factor of activated T cells, cytoplasmic 1, thereby downregulating osteoclast-specific gene (Atp6v0d2, DC-STAMP and cathepsin K) expression. Thus, our results suggest that WCM and WCM-PE are promising nutraceutical candidates for the management of osteoporosis in postmenopausal women.

scholarly journals Immune Cell Population in Ovarian Tumor Microenvironment

2017 ◽  
Vol 8 (15) ◽  
pp. 2915-2923 ◽  
Author(s):  
Dong Li Cai ◽  
Li-Ping Jin
Keyword(s):  
Tumor Microenvironment ◽  
Cell Population ◽  
Ovarian Tumor ◽  
Immune Cell ◽  
Immune Cell Population

Identification and Functional Characterization of Metabolites for Bone Mass in Peri-/Post-menopausal Chinese Women

2021 ◽  
Author(s):  
Rui Gong ◽  
Hong-Mei Xiao ◽  
Yin-Hua Zhang ◽  
Qi Zhao ◽  
Kuan-Jui Su ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Bone Loss ◽  
Osteoclast Differentiation ◽  
Dodecanoic Acid ◽  
Mineral Density ◽  
Ovariectomized Mice ◽  
Post Menopausal ◽  
Functional Mechanisms

Abstract Context Although metabolic profiles appear to play an important role in menopausal bone loss, the functional mechanisms by which metabolites influence bone mineral density (BMD) during menopause are largely unknown. Objective We aimed to systematically identify metabolites associated with BMD variation and their potential functional mechanisms in peri-/post-menopausal women. Design and Methods We performed serum metabolomic profiling and whole-genome sequencing for 517 perimenopausal (16%) and early postmenopausal (84%) women aged 41 to 64 years in this cross-sectional study. Partial least squares (PLS) regression and general linear regression analysis were applied to identify BMD-associated metabolites, and weighted gene co-expression network analysis was performed to construct co-functional metabolite modules. Furthermore, we performed Mendelian randomization analysis to identify causal relationships between BMD-associated metabolites and BMD variation. Finally, we explored the effects of a novel prominent BMD-associated metabolite on bone metabolism through both in vivo/in vitro experiments. Results Twenty metabolites and a co-functional metabolite module (consisting of fatty acids) were significantly associated with BMD variation. We found dodecanoic acid (DA), within the identified module, causally decreased total hip BMD. Subsequently, the in vivo experiments might support that dietary supplementation with DA could promote bone loss, as well as increase the osteoblast and osteoclast numbers in normal/ovariectomized mice. DA treatment differentially promoted osteoblast and osteoclast differentiation, especially for osteoclast differentiation at higher concentrations in vitro (e.g.,10, 100μM). Conclusions This study sheds light on metabolomic profiles associated with postmenopausal osteoporosis risk, highlighting the potential importance of fatty acids, as exemplified by DA, in regulating BMD.

Abstract 003: Cholinergic Expansion of an Inflammatory Macrophage Population in the Pre-Hypertensive Spontaneously Hypertensive Rat

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Sailesh Harwani ◽  
Mark W Chapleau ◽  
Fayyaz Sutterwala ◽  
Zuhair Ballas ◽  
David Meyerholz ◽  
...  
Keyword(s):  
Cell Population ◽  
Immune Cell ◽  
Spontaneously Hypertensive Rat ◽  
Isolated Cells ◽  
Immune Cell Population ◽  
Macrophage Population ◽  
Spontaneously Hypertensive ◽  
Hypertensive Rat ◽  
Wistar Kyoto ◽  
Cholinergic Activation

Our laboratory previously identified an abnormally elevated CD161a+ immune cell population in splenocytes of the pre-hypertensive Spontaneously Hypertensive Rat (SHR) that was abnormally expanded following cholinergic activation with nicotine . In the present study, we tested the hypothesis that the expanded CD161a+ cell population represents an activated monocyte/macrophage population and are present in the bone marrow (BM). We isolated cells from the spleen and BM of pre-hypertensive (4-5 week old) SHR (n=3) and age-matched normotensive Wistar Kyoto (WKY, n=3) rats. Isolated cells were stained with a fluorochrome conjugated anti-rat CD161a monoclonal antibody and analyzed by flow cytometry. CD161a+ cells were more prevalent in splenocytes and BM of the pre-hypertensive SHR, compared to WKY (8.7 ± 1.4% vs 1.2 ± 0.6% and 12.6 ± 1.8% vs 1.7 ± 0.8%, respectively, p<0.001). BM cells and splenocytes were cultured for 36-48 hours in the presence or absence of nicotine (10μM) and then stained with fluorochrome conjugated anti-rat CD161a and CD68 antibodies. CD68 is a well accepted pan-macrophage marker that is up-regulated on activated monocytes/macrophages. The CD161+/CD68+ macrophages were comparable between the WKY and SHR in freshly isolated cells from the spleen (0.3 ± 0.2% vs 0.9 ± 0.5%, respectively) and BM (0.6 ± 0.4% vs 0.7 ± 0.2%, respectively). However, nicotine strongly expanded the CD161a+/CD68+ macrophage population in the BM of the SHR (1.1 ± 0.15% to 11.9 ± 3.45%, p<0.001) and WKY (1.7 ± 0.17% to 11.0 ± 1.9%, p<0.001). In contrast, the response of splenocytes to nicotine was strong in SHR (1.8 ± 0.40% to 10.7 ± 1.1%, p<0.001), but not significant in WKY (1.9 ± 0.4% to 3.1 ± 0.6%, p>0.05). Nicotine had no effect on the CD161a+/CD68- cell population in either splenocytes or BM cells of the SHR or WKY. Thus, an activated monocyte/macrophage population (CD161a+/CD68+) is expanded by cholinergic activation in both the BM and spleen of SHR, but only in the BM and not spleen of the WKY. Since, the pro-inflammatory nicotinic response present in the BM of the WKY is abrogated in the peripheral spleen, we conclude that the retention of this response in the SHR may contribute to the development of hypertension.

Abstract A57: βhCG regulates immune cell population in BRCA1 mutated breast cancers

2020 ◽  
Author(s):  
Geetu Rose Varghese ◽  
Vishnu Sunil Jaikumar ◽  
Arathi Rajan ◽  
Neetha Rajan Latha ◽  
Dipyaman Patra ◽  
...  
Keyword(s):  
Cell Population ◽  
Immune Cell ◽  
Breast Cancers ◽  
Immune Cell Population
Sign in / Sign up

Export Citation Format

Share Document