scholarly journals Glucocorticoid-Responsive Cold Agglutinin Disease in a Patient with Rheumatoid Arthritis

2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Kyoko Honne ◽  
Takao Nagashima ◽  
Masahiro Iwamoto ◽  
Toyomi Kamesaki ◽  
Seiji Minota
Keyword(s):  
Rheumatoid Arthritis ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Bovine Serum ◽  
Cold Agglutinin ◽  
Cold Agglutinin Disease ◽  
Cold Agglutinins ◽  
Thermal Amplitude

A 57-year-old man with rheumatoid arthritis developed severe anemia during treatment with adalimumab plus methotrexate. Cold agglutinin disease was diagnosed because haptoglobin was undetectable, cold agglutinin was positive (1 : 2048), and the direct Coombs test was positive (only to complement). Although the cold agglutinin titer was normalized (1 : 64) after treatment with prednisolone (0.7 mg/kg/day for two weeks), the patient’s hemoglobin did not increase above 8 g/dL. When cold agglutinins were reexamined using red blood cells suspended in bovine serum albumin, the titer was still positive at 1 : 1024. Furthermore, the cold agglutinin had a wide thermal amplitude, since the titer was 1 : 16 at 30°C and 1 : 1 at 37°C. This suggested that the cold agglutinin would show pathogenicity even at body temperature. After the dose of prednisolone was increased to 1 mg/kg/day, the patient’s hemoglobin rapidly returned to the normal range. The thermal amplitude test using red blood cells suspended in bovine serum albumin is more sensitive than the standard test for detecting pathogenic cold agglutinins.

Trypanosoma musculi infection: depression of immune response to heterologous antigens

1981 ◽  
Vol 27 (9) ◽  
pp. 893-898
Author(s):  
M. C. Lajeunesse ◽  
P. Viens
Keyword(s):  
Immune Response ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Bovine Serum ◽  
Contact Sensitivity ◽  
Spleen Cells ◽  
Antibody Titers ◽  
Heterologous Antigens

The response to dinitrofluorobenzene (DNFB), bovine serum albumin (BSA), and sheep red blood cells (SRBC) was studied in CBA mice infected with Trypanosoma musculi at days ranging from 3 to 63 days of infection. DNFB contact sensitivity was normal. In 3-, 10-, and 15-day infected mice, anti-BSA hemagglutinating antibody titers were lower than in noninfected controls when determined after 21 days of BSA challenge, and were normal in sera collected after 10 and 14 days. In 14 day infected mice, anti-SRBC indirect plaque-forming cells (PFC) (per million of nucleated spleen cells and per total spleen) were strongly diminished, but anti-SRBC direct PFC were diminished only per million of nucleated spleen cells. In T. musculi recovered mice, the response to DNFB, BSA, and SRBC was normal.

Comparative intermolecular cross-relaxation studies of human hemoglobin in red blood cells and bovine serum albumin in solution

2011 ◽  
Vol 24 (5) ◽  
pp. 483-491 ◽  
Author(s):  
Seiichi Era ◽  
Masaru Sogami ◽  
Nobuhiro Uyesaka ◽  
Kazuo Kato ◽  
Masataka Murakami ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Bovine Serum ◽  
Cross Relaxation ◽  
Human Hemoglobin ◽  
Relaxation Studies

scholarly journals Beware of Antibodies to Dietary Proteins in “Antigen-specific” Immunoassays! Falsely Positive Anticytokine Antibody Tests Due to Reactivity with Bovine Serum Albumin in Rheumatoid Arthritis (The Swedish TIRA Project)

2010 ◽  
Vol 38 (2) ◽  
pp. 215-220 ◽  
Author(s):  
CHRISTOPHER SJÖWALL ◽  
ALF KASTBOM ◽  
GUNNEL ALMROTH ◽  
JONAS WETTERÖ ◽  
THOMAS SKOGH
Keyword(s):  
Rheumatoid Arthritis ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Blocking Agent ◽  
Strongly Correlated ◽  
Disease Course ◽  
Antibody Levels ◽  
Antibody Tests ◽  
Necrosis Factor

Objective.To evaluate (1) to what extent sera from healthy subjects and patients with rheumatoid arthritis (RA) contain antibodies to bovine serum albumin (BSA); and (2) if anti-BSA antibodies interfere with results of enzyme-linked immunoassays (ELISA) containing BSA.Methods.The ELISA used was a previously developed in-house assay of autoantibodies to tumor necrosis factor (TNF). Anti-TNF and anti-BSA antibodies were analyzed by ELISA in 189 patients with early RA and 186 healthy blood donors. TNF preparations containing either BSA or human serum albumin (HSA) as carrier proteins were used as antigens in the anti-TNF assay. The presence and levels of antibodies were analyzed in relation to disease course and to the presence/absence of rheumatoid factor (RF).Results.In patients with RA, anti-TNF/BSA levels strongly correlated with anti-BSA levels (r = 0.81, p < 0.001), whereas anti-TNF/HSA did not (r = −0.09). Neither the presence nor the levels of anti-BSA in RA patients were associated with disease progression, and antibody levels were not significantly altered compared to controls (p = 0.11). IgG reactivity with TNF/HSA was neglible. In paired sera, preincubation with BSA abolished the anti-TNF/BSA reactivity. There were no indications of RF interference with anti-BSA or anti-TNF reactivity.Conclusion.Antibodies to BSA are common in patients with RA as well as in healthy individuals. Their presence does not seem to be associated with RA disease activity or disease course, but may severely interfere with ELISA containing BSA. The use of BSA as a “blocking agent” or carrier protein in immunoassays should therefore be avoided.

Synthesis, Anti-Arthritic, and Anti-Inflammatory Activity of N-Tosyl aza Cyclophanes

2012 ◽  
Vol 65 (2) ◽  
pp. 186 ◽  
Author(s):  
Perumal Rajakumar ◽  
Ramar Padmanabhan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blood Cells ◽  
Bovine Serum ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Anti Inflammatory

The synthesis of novel N-tosyl tetraaza cyclophanes and N-tosyl diaza cyclophane incorporating m-terphenyl as spacer units is described. Anti-arthritic activity was studied by inhibition of the protein denaturation method (bovine serum albumin). All the N-tosyl aza cyclophanes exhibit excellent anti-arthritic activity. Anti-inflammatory activity of the synthesized cyclophanes was investigated using the human red blood cells (HRBC) membrane stabilization method and some of the N-tosyl aza cyclophanes exhibited good anti-inflammatory activity.

In vitro binding of dantrolene to bovine serum albumin and rabbit red blood cell ghosts

1982 ◽  
Vol 60 (10) ◽  
pp. 1307-1311 ◽  
Author(s):  
A. R. Dehpour ◽  
M. Mahmoudian
Keyword(s):  
Bovine Serum Albumin ◽  
Blood Cell ◽  
Serum Albumin ◽  
Red Blood Cell ◽  
Binding Sites ◽  
Blood Cells ◽  
Bovine Serum ◽  
Association Constant ◽  
Dantrolene Sodium

The binding of dantrolene to rabbit red blood cell (RBC) ghosts and bovine serum albumin as models of receptor sites was studied using fluorescence techniques. Dantrolene upon binding to rabbit RBC ghosts and bovine serum albumin showed fluorescence with emission maxima at 495 and 500 nm, respectively. Dantrolene bound to rabbit RBC ghosts with an association constant of 6.06 × 104 M−1 and there were 2.4 × 106 dantrolene binding sites per ghost. The binding of dantrolene to bovine serum albumin showed an anomaly with respect to dantrolene concentration. Dantrolene at concentrations of 1.25 – 3.75 μM bound to bovine serum albumin with an association constant of 1.72 × 104 M−1 and there were 1.078 binding sites/mol bovine serum albumin. The higher affinity of dantrolene toward blood cells was confirmed by studying the distribution of dantrolene between blood cells and plasma after a single i.p. injection of dantrolene sodium (3 mg/kg) to albino rabbits. It was found that the concentration of dantrolene in rabbit blood cells was 2.76 times that of plasma.

scholarly journals Cold agglutinin disease related mismatch between hematocrit and hemoglobin values

2020 ◽  
Vol 7 (2) ◽  
pp. 770
Author(s):  
Emre Hoca ◽  
Fatma Pınar Ziyadanoğlu ◽  
Atay Can Kula ◽  
Hayriye Esra Ataoğlu
Keyword(s):  
Autoimmune Diseases ◽  
Autoimmune Hemolytic Anemia ◽  
Blood Cells ◽  
Epstein Barr Virus ◽  
Cold Agglutinin ◽  
Cold Agglutinin Disease ◽  
Cold Agglutinins ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

Autoimmune hemolytic anemia is a disease characterized by destruction of red blood cells (RBC) and anemia, caused by production of antibodies released against the body’s own RBCs. While this condition is more commonly idiopathic, it may accompany autoimmune diseases as well. Cold agglutinin disease (CAD) is a rare subtype of acquired autoimmune hemoliytic anemia, however, is an idiosyncratic clinical and pathological terminology, usually seen in older ages. This condition is caused by IgM antibodies called “cold agglutinins” formed against I antigens on RBC membranes which cause agglutination of RBCs at lower temperatures. In this case, a 56 year old male patient who was diagnosed with CAD secondary to Epstein-Barr virus (EBV) infection is being presented.

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