scholarly journals Increased Frequency of CD4+ CD25+ FoxP3+ T Regulatory Cells in Pulmonary Tuberculosis Patients Undergoing Specific Treatment and Its Relationship with Their Immune-Endocrine Profile

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Ariana Díaz ◽  
Natalia Santucci ◽  
Bettina Bongiovanni ◽  
Luciano D’Attilio ◽  
Claudia Massoni ◽  
...  
Keyword(s):  
T Regulatory Cells ◽  
Specific Treatment ◽  
Cell Immune Response ◽  
Regulatory Cells ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Tb Treatment ◽  
Endocrine Profile

Tuberculosis (TB) is a major health problem requiring an appropriate cell immune response (IR) to be controlled. Since regulatory T cells (Tregs) are relevant in IR regulation, we analyzed Tregs variations throughout the course of TB treatment and its relationship with changes in immune-endocrine mediators dealing with disease immunopathology. The cohort was composed of 41 adult patients, 20 of them completing treatment and follow-up. Patients were bled at diagnosis (T0) and at 2 (T2), 4 (T4), 6 (T6), and 9 months following treatment initiation. Twenty-four age- and sex-matched healthy controls (HCo) were also included. Tregs (flow cytometry) from TB patients were increased at T0 (versus HCoP<0.05), showing even higher values at T2 (versus T0P<0.01) and T4 (versus T0P<0.001). While IL-6, IFN-γ, TGF-β(ELISA), and Cortisol (electrochemiluminescence, EQ) were augmented, DHEA-S (EQ) levels were diminished at T0 with respect to HCo, with cytokines and Cortisol returning to normal values at T9. Tregs correlated positively with IFN-γ(R=0.868,P<0.05) at T2 and negatively at T4 (R=-0.795,P<0.05). Lowered levels of proinflammatory cytokines together with an increased frequency of Tregs of patients undergoing specific treatment might reflect a downmodulatory effect of these cells on the accompanying inflammation.

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

scholarly journals Brain Studded with Innumerable Larger Stones: Starry Sky at Night Appearance: Multiple Calcified Giant Tuberculoma with 15 Years of Follow-up

2017 ◽  
Vol 06 (02) ◽  
pp. 155-158
Author(s):  
Guru Satyarthee ◽  
P. Chandra ◽  
A. Mahapatra
Keyword(s):  
Central Nervous System Involvement ◽  
Definitive Diagnosis ◽  
Shunt Surgery ◽  
Unique Case ◽  
Major Health Problem ◽  
Major Health ◽  
Vp Shunt ◽  
Cranial Ct ◽  
Rising Incidence

AbstractTuberculosis is rapidly reemerging as a major health problem due to rising incidence of HIV cases across the globe. Central nervous system involvement is rare, but rarely multiple intracerebral granulomas can occur or occasional solitary tuberculoma also develops. The authors report a unique case of 24-year-old woman suffering from pulmonary tuberculosis developed headache and vomiting. Cranial CT scan revealed multiple widespread deposit of intracranial granuloma of sizeable lesion mimicking starry sky at night appearance. Excision of one tuberculoma was done for confirmation of definitive diagnosis, and histopathology was suggestive of tuberculoma. The patient also underwent VP shunt surgery. At last follow-up at 15 years following surgery, she was doing well.

scholarly journals Chemical Dependence in Anaesthetic Registrars in Australia and New Zealand

1993 ◽  
Vol 21 (2) ◽  
pp. 151-155 ◽  
Author(s):  
A. M. Weeks ◽  
M. R. Buckland ◽  
E. B. Morgan ◽  
P. S. Myles
Keyword(s):  
New Zealand ◽  
Health Problem ◽  
Chemical Dependence ◽  
Chemically Dependent ◽  
Major Health Problem ◽  
Major Health

The Supervisors of Anaesthetic Training in Australia and New Zealand were surveyed and asked to report any cases of chemical dependence from anaesthetic registrars at their hospital from 1981 to 1991. From 83 questionnaires there were 65 (78%) returned. There were 14 departments (22%) with experience of one or more cases during this interval. Only five (7%) reported conducting a lecture or tutorial on the problem. The departmental reports covered 4425 registrar years of training and there were 17 cases reported. Of these, 13 were complete and are considered in detail. It is estimated that 1.3 % of those who entered anaesthetic training during the interval were recognised to become chemically dependent during their training. Follow-up was available on only six of the 13 registrars and only one was reported to have completed training. The results of this survey indicate that chemical dependence is already a major health problem amongst anaesthetic registrars in Australia and New Zealand.

scholarly journals Perforated uterus

2018 ◽  
Vol 3 (1) ◽  
pp. 46-47
Author(s):  
Justus W Ngatia
Keyword(s):  
Early Detection ◽  
Pregnancy Loss ◽  
Uterine Perforation ◽  
Potential Complication ◽  
Major Health Problem ◽  
Major Health ◽  
Pregnancy Failure ◽  
Dilatation And Curettage ◽  
Early Pregnancy Failure

Early pregnancy failure is a major health problem worldwide which occurs in 15-20% of pregnancies. During evacuation, uterine perforation is a potential complication. Careful post-evacuation follow-up helps in early detection of perforation. Perforation often requires laparotomy or laparoscopy to repair the defect and evaluate for injury to adjacent organs. Our patient had pregnancy loss at 12 weeks and uterine perforation during a dilatation and curettage procedure. She had laparotomy, evacuation through the perforation site and uterine repair with good outcome.

scholarly journals Complete Stenosis of the Uretero Vesical Junction in Urogenital Tuberculosis: Management and Evolution

2021 ◽  
Vol 3 (1) ◽  
pp. 21-23
Author(s):  
O. Elidrissi ◽  
M. Rkik ◽  
W. Bai ◽  
Y. Ghannam ◽  
M. Dakir ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Health Problem ◽  
Disease Type ◽  
Ureteral Stenosis ◽  
Major Health Problem ◽  
Major Health ◽  
Urogenital Tuberculosis ◽  
The World

Urogenital tuberculosis continues to be a major health problem around the world due to the epidemic of HIV infection. We report the case of a 40-year-old patient followed for urogenital tuberculosis and who presented during her follow-up a complication of her disease type ureteral stenosis, managed by endoscopic route. We try through our work to focus on the value of surveillance after anti-bacillary treatment in order to watch for complications and act in an innocuous way.

scholarly journals Assessment of Neopterin Serum Level for Monitoring Therapy of Patients with Mycobacterium Tuberculosis

2021 ◽  
Vol 13 (3) ◽  
Author(s):  
Shokrollah Salmanzadeh ◽  
Razieh Mombeini ◽  
Seyed Mohammad Alavi ◽  
Morteza Abdullatif Khafaie
Keyword(s):  
Serum Levels ◽  
Response To Treatment ◽  
Sputum Smear ◽  
Major Health Problem ◽  
Tb Treatment ◽  
Monitoring Therapy ◽  
Significant Difference ◽  
The Mean ◽  
First Time

Background: Tuberculosis (TB) is a major health problem worldwide. To date, the standard diagnostic method for TB is still the direct observation of Mycobacterium TB in a sputum smear or culture. Objectives: There is an urgent need for a method to detect the disease in a shorter time with acceptable sensitivity and specificity and help monitor the treatment. Methods: A panel of 34 adults newly diagnosed with smear-positive pulmonary TB was followed during their treatment period of five months. Neopterin (NPT) serum levels were measured three times (before treatment and two and five months after treatment) using the Brahms ELItest Neopterin kit, and the results were analyzed using descriptive and graphical methods. Results: The mean NPT for the first time before treatment was 27.47 ± 20.7 nmol/L. NPT was measured two months after the anti-TB treatment, and the associated mean was 16.97 ± 14.14 nmol/L. At the end of the fifth month, the mean NPT concentration reached 11.3 ± 10.5 nmol/L. The mean serum NPT before treatment had a statistically significant difference with the mean NPT at the end of the second and fifth months of the treatment (P = 0.001). Conclusions: According to the national protocol for treating TB, a sputum smear is still necessary for patients’ follow-up. It seems that the serum NPT level should be measured to monitor response to treatment in patients that, for any reason are not able to give sputum in TB treatment follow-up.

scholarly journals Human T Cell and Antibody-Mediated Responses to theMycobacterium tuberculosisRecombinant 85A, 85B, and ESAT-6 Antigens

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Gilson C. Macedo ◽  
Adriana Bozzi ◽  
Helena Rachel Weinreich ◽  
Andre Bafica ◽  
Henrique C. Teixeira ◽  
...  
Keyword(s):  
Healthy Individuals ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Cellular Immune Responses ◽  
Human T Cell ◽  
Tnf Α ◽  
Ifn Γ ◽  
Cellular Immune ◽  
Control And Eradication

Tuberculosis remains a major health problem throughout the world causing large number of deaths. Effective disease control and eradication programs require the identification of major antigens recognized by the protective responses againstM. tuberculosis. In this study, we have investigated humoral and cellular immune responses toM. tuberculosis-specific Ag85A, Ag85B, and ESAT-6 antigens in Brazilian patients with pulmonary (P,n=13) or extrapulmonary (EP,n=12) tuberculosis, patients undergoing chemotherapy (PT,n=23), and noninfected healthy individuals (NI,n=7). Compared to NI, we observed increased levels of IgG1 responses to Ag85B and ESAT-6 in P and PT groups. Regarding cellular immunity, Ag85A and ESAT-6 were able to discriminate P, PT, and EP patients from healthy individuals by IFN-γ production and P and PT groups from EP individuals by production of TNF-α. In summary, these findings demonstrate the ability of Ag85A, Ag85B, and ESAT-6 to differentiate TB patients from controls by IgG1, IFN-γ and TNF-α production.

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