scholarly journals The Ability of Quantitative, Specific, and Sensitive Point-of-Care/Chair-Side Oral Fluid Immunotests for aMMP-8 to Detect Periodontal and Peri-Implant Diseases

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Saeed Alassiri ◽  
Pirjo Parnanen ◽  
Nilminie Rathnayake ◽  
Gunnar Johannsen ◽  
Anna-Maria Heikkinen ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Gingival Crevicular Fluid ◽  
Point Of Care ◽  
High Sensitivity ◽  
Mouth Rinse ◽  
Treatment And Prevention ◽  
Oral Fluids ◽  
Health And Disease ◽  
Crevicular Fluid ◽  
Disease Specific

The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth rinse, gingival crevicular fluid (GCF), and peri-implantitis fluid (PISF)) is demanding. Several hosts and microbial factors may influence their expression, release, and levels. The type of saliva/oral fluids utilized for the diagnostics affects the analysis. High sensitivity and specificities together with sophisticated methods and techniques are essential for valuable outcome. We describe here recently developed practical, convenient, inexpensive, noninvasive, and quantitative mouth rinse and PISF/GCF/chair-side/point-of-care (PoC) lateral-flow aMMP-8 immunoassays (PerioSafe and ImplantSafe/ORALyser) to detect, predict, and monitor successfully the course, treatment, and prevention of periodontitis and peri-implantitis, respectively. The tests have been independently and successfully validated to differentiate periodontal and peri-implant health and disease in Finland, Germany, Netherland, Sweden, Turkey, Nigeria, Malawi, and USA. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires additional studies utilizing these noninvasive screening, diagnostic, and preventive aMMP-8 PoC/chair-side technologies.

scholarly journals Matrix Metalloproteinase-8 as an Inflammatory and Prevention Biomarker in Periodontal and Peri-Implant Diseases

2018 ◽  
Vol 2018 ◽  
pp. 1-27 ◽  
Author(s):  
Ahmed Al-Majid ◽  
Saeed Alassiri ◽  
Nilminie Rathnayake ◽  
Taina Tervahartiala ◽  
Dirk-Rolf Gieselmann ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Literature Search ◽  
Gingival Crevicular Fluid ◽  
Mouth Rinse ◽  
Strong Potential ◽  
Oral Fluids ◽  
Crevicular Fluid

Levels of and especially the degree of activation of matrix metalloproteinase (MMP-8) in oral fluids (i.e., saliva, mouth rinse, gingival crevicular fluid (GCF) and peri-implantitis sulcular fluid (PISF)) increase to pathologically elevated levels in the periodontal and peri-implant diseases. This study aimed at collecting and collating data from previously published studies and determining whether active MMP-8 (aMMP-8) could serve as a biomarker for the diagnosis and prevention of periodontal and peri-implant diseases. The literature search identified a total of 284 articles. Out of 284 articles, 61 articles were found to be relevant. Data obtained from the selected studies were combined, and it indicated that aMMP-8 in oral fluids exerts the strong potential to serve as a useful adjunctive diagnostic and preventive biotechnological tool in periodontal and peri-implant diseases. aMMP-8 can be used alone or in combination with other proinflammatory and/or microbiological biomarkers.

Trends in Diagnosis for Active Tuberculosis Using Nanomaterials

2019 ◽  
Vol 26 (11) ◽  
pp. 1946-1959 ◽  
Author(s):  
Le Minh Tu Phan ◽  
Lemma Teshome Tufa ◽  
Hwa-Jung Kim ◽  
Jaebeom Lee ◽  
Tae Jung Park
Keyword(s):  
Sensitivity And Specificity ◽  
High Efficiency ◽  
Point Of Care ◽  
High Sensitivity ◽  
Signs And Symptoms ◽  
Diagnostic Methods ◽  
Advantages And Disadvantages ◽  
Treatment And Prevention ◽  
Clinical Tools ◽  
Diagnostic Applications

Background:Tuberculosis (TB), one of the leading causes of death worldwide, is difficult to diagnose based only on signs and symptoms. Methods for TB detection are continuously being researched to design novel effective clinical tools for the diagnosis of TB.Objective:This article reviews the methods to diagnose TB at the latent and active stages and to recognize prospective TB diagnostic methods based on nanomaterials.Methods:The current methods for TB diagnosis were reviewed by evaluating their advantages and disadvantages. Furthermore, the trends in TB detection using nanomaterials were discussed regarding their performance capacity for clinical diagnostic applications.Results:Current methods such as microscopy, culture, and tuberculin skin test are still being employed to diagnose TB, however, a highly sensitive point of care tool without false results is still needed. The utilization of nanomaterials to detect the specific TB biomarkers with high sensitivity and specificity can provide a possible strategy to rapidly diagnose TB. Although it is challenging for nanodiagnostic platforms to be assessed in clinical trials, active TB diagnosis using nanomaterials is highly expected to achieve clinical significance for regular application. In addition, aspects and future directions in developing the high-efficiency tools to diagnose active TB using advanced nanomaterials are expounded.Conclusion:This review suggests that nanomaterials have high potential as rapid, costeffective tools to enhance the diagnostic sensitivity and specificity for the accurate diagnosis, treatment, and prevention of TB. Hence, portable nanobiosensors can be alternative effective tests to be exploited globally after clinical trial execution.

scholarly journals Application of Vibrational Spectroscopies in the Qualitative Analysis of Gingival Crevicular Fluid and Periodontal Ligament during Orthodontic Tooth Movement

2021 ◽  
Vol 10 (7) ◽  
pp. 1405
Author(s):  
Fabrizia d’Apuzzo ◽  
Ludovica Nucci ◽  
Ines Delfino ◽  
Marianna Portaccio ◽  
Giuseppe Minervini ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
High Sensitivity ◽  
Data Analysis Procedure ◽  
Vibrational Spectroscopies ◽  
Composition Structure ◽  
The Individual ◽  
Crevicular Fluid

Optical vibrational techniques show a high potentiality in many biomedical fields for their characteristics of high sensitivity in revealing detailed information on composition, structure, and molecular interaction with reduced analysis time. In the last years, we have used these techniques for investigating gingival crevicular fluid (GCF) and periodontal ligament (PDL) during orthodontic tooth treatment. The analysis with Raman and infrared signals of GCF and PDL samples highlighted that different days of orthodontic force application causes modifications in the molecular secondary structure at specific wavenumbers related to the Amide I, Amide III, CH deformation, and CH3/CH2. In the present review, we report the most relevant results and a brief description of the experimental techniques and data analysis procedure in order to evidence that the vibrational spectroscopies could be a potential useful tool for an immediate monitoring of the individual patient’s response to the orthodontic tooth movement, aiming to more personalized treatment reducing any side effects.

Evaluation of Immunoassay-Based MMP-8 Detection in Gingival Crevicular Fluid on a Point-of-Care Platform

2007 ◽  
Vol 1098 (1) ◽  
pp. 490-492 ◽  
Author(s):  
S. K MUNJAL ◽  
N PRESCHER ◽  
F STRUCK ◽  
T SORSA ◽  
K MAIER ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Point Of Care ◽  
Crevicular Fluid

Gingival Crevicular Fluid Levels of Leukotriene B4in Periodontal Health and Disease

2007 ◽  
Vol 78 (12) ◽  
pp. 2325-2330 ◽  
Author(s):  
A.R. Pradeep ◽  
S.G. Manjunath ◽  
Pradeep Patel Swati ◽  
Chowdhry Shikha ◽  
Pai B. Sujatha
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Health ◽  
Health And Disease ◽  
Crevicular Fluid ◽  
Fluid Levels

scholarly journals Gingival Crevicular Fluid and Serum Cystatin C Levels in Periodontal Health and Disease

2012 ◽  
Vol 32 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Anuj Sharma ◽  
A. R. Pradeep ◽  
N. M. Raghavendra ◽  
P. Arjun ◽  
Rahul Kathariya
Keyword(s):  
Cystatin C ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Therapy Group ◽  
Periodontal Diseases ◽  
Periodontal Therapy ◽  
Periodontal Health ◽  
Healthy Group ◽  
Health And Disease ◽  
Crevicular Fluid

Cystatin C (CSTC) is an inhibitor of cysteine proteinases and could play a protective and regulatory role under inflammatory conditions. The present study was designed to assess the concentration of CSTC in gingival crevicular fluid (GCF) and serum, to find out their association if any, in periodontal health and disease. 30 subjects were selected divided into 3 groups consisting of 10 subjects in each group based on clinical parameters: periodontally healthy group, gingivitis group and chronic periodontitis group, while, chronic periodontitis group after 8 weeks of the treatment (scaling and root planing) constituted after periodontal therapy group. GCF and serum samples were collected from all subjects to estimate the levels of CSTC by ELISA. The mean CSTC concentration in GCF and serum was observed to be the highest in periodontitis group and lowest in periodontally healthy group with intermediate concentration in gingivitis group and after periodontal therapy group. CSTC concentration in GCF and serum increased proportionally with the severity of periodontal disease (from health to periodontitis group) and decreased after treatment. This suggests that CSTC increases with disease progression to prevent further periodontal degeneration and decreases after treatment due to bone metabolic homeostasis. Further, longitudinal prospective studies involving larger population are needed to confirm the findings of present study and to better understand the role of CSTC in the pathogenesis of periodontal diseases.

Quantitative analysis of MRP-8 in gingival crevicular fluid in periodontal health and disease using microbore HPLC

2001 ◽  
Vol 28 (12) ◽  
pp. 1172-1177 ◽  
Author(s):  
Fionnuala T. Lundy ◽  
Rod Chalk ◽  
Philip-John Lamey ◽  
Christopher Shaw ◽  
Gerard J. Linden
Keyword(s):  
Quantitative Analysis ◽  
Gingival Crevicular Fluid ◽  
Periodontal Health ◽  
Health And Disease ◽  
Crevicular Fluid
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