scholarly journals Effects of Antimicrobial Photodynamic Therapy and Local Administration of Minocycline on Clinical, Microbiological, and Inflammatory Markers of Periodontal Pockets: A Pilot Study

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Takahiro Hokari ◽  
Toshiya Morozumi ◽  
Yasutaka Komatsu ◽  
Taro Shimizu ◽  
Toshiaki Yoshino ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Interferon Γ ◽  
Local Administration ◽  
Clinical Parameters ◽  
Antimicrobial Photodynamic Therapy ◽  
Local Host ◽  
Crevicular Fluid ◽  
Clinical Trials Registry

Objective. We evaluated the efficacies of antimicrobial photodynamic therapy (aPDT) and minocycline ointment (MO) on clinical and bacteriological markers and the local host inflammatory response. Materials and Methods. A total of 30 patients with chronic periodontitis were randomly assigned to two groups. Selected periodontal pockets (probing depth 5–7 mm with bleeding on probing) were treated with aPDT or MO. Measurements of clinical parameters and the collection of gingival crevicular fluid (GCF) and subgingival plaque were performed at baseline, and at 1 and 4 weeks after treatment. Quantification of periodontopathic bacteria in the sulcus and a multiplex bead immunoassay of ten inflammatory cytokines in the GCF were performed. Results. Local MO administration exhibited a significant decrease in scores for clinical parameters (P<0.01) and a significant reduction in bacterial counts (P<0.01) and interleukin-1β and interferon-γ levels at 1 and 4 weeks after treatment (P<0.01). No significant changes were observed in the aPDT group, except in clinical parameters. Conclusions. Although our study had some limitations, we found that while local administration of MO may slightly help to improve clinical, microbiological, and crevicular cytokine levels in periodontal pockets, aPDT did not show any effects. This trial is registered with the UMIN Clinical Trials Registry UMIN000013376.

Effects of Antimicrobial Photodynamic Therapy on Transforming Growth Factor-β1 Levels in the Gingival Crevicular Fluid

2013 ◽  
Vol 31 (2) ◽  
pp. 65-71 ◽  
Author(s):  
Sérgio L. S. Souza ◽  
Patrícia F. Andrade ◽  
João S. Silva ◽  
Fabrine S. M. Tristão ◽  
Fernanda A. Rocha ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Gingival Crevicular Fluid ◽  
Transforming Growth Factor Β1 ◽  
Antimicrobial Photodynamic Therapy ◽  
Crevicular Fluid

scholarly journals Microbiota in Gingival Crevicular Fluid Before and After Mechanical Debridement With Antimicrobial Photodynamic Therapy in Peri-Implantitis

2022 ◽  
Vol 11 ◽  
Author(s):  
Haiyan Wang ◽  
Ye Liu ◽  
Wei Li ◽  
Wenyue Li ◽  
Hongtao Xu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Treatment Group ◽  
Gingival Crevicular Fluid ◽  
Reference Database ◽  
Antimicrobial Photodynamic Therapy ◽  
Significant Difference ◽  
Before And After ◽  
Pre Treatment ◽  
Crevicular Fluid ◽  
Level 2

ObjectivesThis study aims to compare the microbiota of gingival crevicular fluid (GCF) before and after mechanical debridement (MD) with antimicrobial photodynamic therapy (aPDT) and determine the core efficient microbiota in peri-implantitis after treatment.MethodsWe recruited 9 patients (14 implants) treated with MD+aPDT for peri-implantitis at our center from February 1, 2018, to February 1, 2019. GCF was collected using filter paper strip before and after the treatment. The bacterial 16S rRNA was amplified and sequenced using an Illumina MiSeq platform to characterize the GCF. Bioinformatics and statistical analyses were performed using QIIME2 and R.ResultsA total of 4,110,861 high-quality sequences were obtained from GCF samples. Based on the reference database, 1,120 amplicon sequence variants (ASVs) were finally harvested. Principal coordinates analysis indicated significant differences in the bacterial community structure between the 180 days after-treatment group and pre-treatment group. Difference analysis and least discriminant analysis showed that the differences were mainly reflected in non-dominant bacteria between these two groups. The non-dominant genera with significantly different distribution between the 180 days after-treatment group and the pre-treatment group included Lactobacillus, Pedobacter, Bulleidia, Centipeda, Desulfovibrio, Ochrobactrum, Staphylococcus, Microbacterium, Brevundimonas, Desulfobulbus, and Parvimonas. Moreover, a total of 29 predictive functional categories at KEGG level 2 were identified. The significant difference pathways at KEGG level 2 between after-treatment and pre-treatment were concentrated in infectious disease-related pathways.ConclusionsPatients with peri-implantitis have significant changes in the low-abundance bacteria of the GCF before and after MD+aPDT. MD+aPDT may change the composition of GCF microbiota by increasing the abundance of cluster 1 (beneficial) and decreasing that of cluster 4 (harmful), which may decrease metabolic response to infection and thus improve peri-implantitis.

scholarly journals Clinical and Biological Effects of Adjunctive Photodynamic Therapy in Refractory Periodontitis

2019 ◽  
Vol 10 (2) ◽  
pp. 139-145
Author(s):  
Rola Al Habashneh ◽  
Mohammad A Mashal ◽  
Yousef Khader ◽  
Rana Qudah
Keyword(s):  
Photodynamic Therapy ◽  
Gingival Crevicular Fluid ◽  
Biological Effects ◽  
Gingival Recession ◽  
Periodontal Treatment ◽  
Control Group ◽  
Clinical Parameters ◽  
Biological Parameters ◽  
Probing Depth ◽  
Crevicular Fluid

Introduction: To date, no novel treatment approach is available for optimum outcomes regarding refractory periodontitis. The aim of the present study was to assess the efficiency of photodynamic therapy (PDT) in treating patients diagnosed with refractory periodontitis and compare the clinical and biological outcomes of conventional periodontal treatment with or without adjunctive PDT in these patients, by assessing clinical parameters (plaque index [PI], gingival recession [GR], bleeding on probing [BOP], periodontal probing depth [PPD] and clinical attachment level [CAL]) as well as biological parameters (IL-1β) in the gingival crevicular fluid (GCF). Methods: Sixteen patients within the age of 30 to 60 years, with a mean age of 40 years old, diagnosed with refractory periodontitis were included. In this split mouth design study, 2 quads (1 upper + 1 lower) from the same patient were randomly treated with (scaling and root planing [SRP]+PDT) together. The other 2 quadrants (1 upper + 1 lower) were treated by SRP only and selected to serve as controls. Clinical parameters including PI, GR, BOP, PPD and CAL and biological parameters (IL-1β) in the GCF were measured at baseline, then at, 2 and 6 months after therapy. Results: A statistically significant reduction in several clinical parameters as, BOP (P < 0.001), PI (P < 0.001), PPD (P < 0.001) and CAL (P < 0.001) in quadrant treated with SRP and adjunctive PDT when compared to control group treated with SRP alone was observed and both therapies showed non-statistically significant differences in the reduction of IL-1β level. Conclusion: The inclusion of PDT as an adjunctive measure to nonsurgical conventional periodontal treatment seems to be a useful therapeutic measure in refractory periodontitis treatment.

Antimicrobial Photodynamic Therapy in the Non-Surgical Treatment of Aggressive Periodontitis: Cytokine Profile in Gingival Crevicular Fluid, Preliminary Results

2009 ◽  
Vol 80 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Rafael Ramos de Oliveira ◽  
Humberto Oswaldo Schwartz-Filho ◽  
Arthur Belém Novaes ◽  
Gustavo Pompermaier Garlet ◽  
Raphael Freitas de Souza ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Surgical Treatment ◽  
Gingival Crevicular Fluid ◽  
Aggressive Periodontitis ◽  
Cytokine Profile ◽  
Antimicrobial Photodynamic Therapy ◽  
Preliminary Results ◽  
Crevicular Fluid ◽  
Non Surgical Treatment

scholarly journals Origin of MMP-8 and Lactoferrin levels from gingival crevicular fluid, salivary glands and whole saliva

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Liza L. Ramenzoni ◽  
Deborah Hofer ◽  
Alex Solderer ◽  
Daniel Wiedemeier ◽  
Thomas Attin ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Salivary Glands ◽  
Gingival Crevicular Fluid ◽  
Clinical Parameters ◽  
Pocket Depth ◽  
Active Matrix ◽  
Probing Pocket Depth ◽  
Whole Saliva ◽  
Biomarker Testing ◽  
Crevicular Fluid

Abstract Background Pathologically elevated levels of matrix metalloproteinase-8 (MMP-8) and Lactoferrin in oral fluids have been associated with the presence of gingivitis/periodontitis. This study aimed to assess the origin of MMP-8 and Lactoferrin in periodontitis patients and to identify the degree to which conventional clinical parameters correlate with their presence. Methods A total of ten periodontitis and ten healthy patients were included in this study. Whole saliva (stimulated and unstimulated), parotid/sublingual glandular fluid and gingival crevicular fluid from pockets and sulci were tested for MMP-8 and Lactoferrin and protein concentrations were quantified using an ELISA assay. Clinical parameters were checked for potential associations with MMP-8 and Lactoferrin levels. Results Periodontal patients presented higher concentrations of MMP-8 and Lactoferrin in pockets than other sources (P = 0.03). Lactoferrin measurement was higher in the parotid compared to sublingual glandular fluid in periodontitis patients (P = 0.03). Increased probing pocket depth was positively correlated with high MMP-8 and Lactoferrin levels. Conclusions Periodontal pockets appear to be the major source of active matrix metalloproteinase and Lactoferrin, which also may also enter the oral cavity through the salivary glands. Since clinically healthy sites in periodontitis patients also had elevated biomarker levels, gingival crevicular fluid biomarker testing may be more predictive of future tissue breakdown than conventional clinical parameters.

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