scholarly journals Emphasized Mechanistic Antimicrobial Study of Biofunctionalized Silver Nanoparticles on Model Proteus mirabilis

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Asra Parveen ◽  
Manjunath S. Yalagatti ◽  
Venkataraman Abbaraju ◽  
Raghunandan Deshpande
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Proteus Mirabilis ◽  
Reducing Sugars ◽  
Radical Scavenging ◽  
Human Pathogens ◽  
Antimicrobial Study ◽  
Synthetic Procedure ◽  
Dpph Method ◽  
The Stability

Antimicrobial study of biofunctionalized silver nanoparticles has been done with the emphasis on its mechanism on both gram positive and negative bacteria. The biofunctionalized silver nanoparticles are employed considering their importance in green chemistry with respect to easy synthesis, usefulness, and economic synthetic procedure involved. The stability of these nanoparticles was determined by zeta potential analyzer. The probable mechanism of antibacterial activity was performed on Proteus mirabilis by field emission scanning electron microscopy (FESEM) and energy dispersive spectroscopy (EDAX) study which does not show the presence of silver. The free radicals generated by silver nanoparticles were responsible for lethal antibacterial activity by rupturing the cell surface which causes improper nutrient and signal supply. Free radical scavenging efficacy of silver nanoparticles was confirmed by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method. AgNP enhanced the membrane leakage of reducing sugars by destroying the proteins existing on the cell wall. These nanoparticles are found to be toxic against human pathogens and are highly effective on Staphylococcus aureus. The effect of silver nanoparticles is concentration dependent and independent of the type of strains used.

scholarly journals Exploiting Fruit Waste Grape Pomace for Silver Nanoparticles Synthesis, Assessing Their Antioxidant, Antidiabetic Potential and Antibacterial Activity Against Human Pathogens: A Novel Approach

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1457 ◽  
Author(s):  
Ganesh Dattatraya Saratale ◽  
Rijuta Ganesh Saratale ◽  
Dong-Su Kim ◽  
Do-Yeong Kim ◽  
Han-Seung Shin
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Radical Scavenging Activity ◽  
Waste Product ◽  
Radical Scavenging ◽  
Grape Pomace ◽  
Wine Industry ◽  
Human Pathogens ◽  
Industry Waste ◽  
Novel Approach

Grape pomace, a most abundant and renewable wine industry waste product was utilized as a suitable reducing, capping, and stabilizing biomolecules for green synthesis of silver nanoparticles (AgNPs). The physicochemical properties of biosynthesized grape pomace extract (GPE)-AgNPs were duly appraised via UV–Visible spectroscopy, X-ray diffractometer (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The analytical studies revealed that the GPE-AgNPs were well formed and stable in nature. The functional groups of organic molecules of GPE are present on the surface of AgNPs with average NPs diameter in the range of 20–35 nm. GPE-AgNPs exhibited significant free radical scavenging activity mainly DPPH radical (IC50, 50.0 ± 2.25 μg/mL) and ABTS radical (IC50, 38.46 ± 1.14 μg/mL). Additionally, the synthesized AgNPs showed noticeable inhibition of carbohydrate hydrolyzing enzymes mainly, α-amylase (IC50, 60.2 ± 2.15 μg/mL) and α-glucosidase (IC50, 62.5 ± 2.75 μg/mL). The GPE fabricated AgNPs showed noteworthy antibacterial potential against infectious bacteria viz., Escherichia coli and Staphylococcus aureus. The reaction mechanism of antibacterial activity was studied by measuring the bacterial cell membrane breakage and cytoplasmic contents, mainly, nucleic acid, proteins, and reducing sugar. Therefore, this research attempt illustrated the potential of GPE as a novel source intended for the biosynthesis of AgNPs that may open up new horizons in the field of nanomedicine.

scholarly journals Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

2019 ◽  
Vol 10 (2) ◽  
pp. 94-98
Author(s):  
Adlis Santoni ◽  
Mai Efdi ◽  
Akmel Suhada
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Endemic Plant ◽  
Dpph Method ◽  
Hexane Extracts

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity  was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibition zone 10 mm at  concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

scholarly journals Biosynthesis of silver nanoparticles from the marine seaweed Sargassum wightii and their antibacterial activity against some human pathogens

2013 ◽  
Vol 4 (7) ◽  
pp. 881-888 ◽  
Author(s):  
N. Shanmugam ◽  
P. Rajkamal ◽  
S. Cholan ◽  
N. Kannadasan ◽  
K. Sathishkumar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Human Pathogens ◽  
Sargassum Wightii

scholarly journals The study of antibacterial activity and stability of dyed cotton fabrics modified with different forms of silver

2012 ◽  
Vol 77 (2) ◽  
pp. 225-234 ◽  
Author(s):  
Vesna Lazic ◽  
Zoran Saponjic ◽  
Vesna Vodnik ◽  
Suzana Dimitrijevic ◽  
Petar Jovancic ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Active Component ◽  
Silver Chloride ◽  
Cotton Fabrics ◽  
Negative Bacterium ◽  
Positive Bacterium ◽  
Vat Dyes ◽  
Artificial Sweat ◽  
The Stability

This study compares the effect of colloidal silver nanoparticles and commercial RUCO-BAC AGP agent with silver chloride as an active component on antibacterial activity of dyed cotton fabrics. Cotton fabrics were dyed with vat dyes Bezanthren olive T and Bezanthren grey FFB. Antibacterial activity of silver loaded dyed cotton fabrics was tested against Gram-positive bacterium Staphylococcus aureus and Gram-negative bacterium Escherichia coli. Unlike RUCO-BAC AGP synthesized silver nanoparticles deposited onto dyed cotton fabrics provided maximum bacteria reduction independently of applied dye. The stability of modified cotton fabrics was analyzed in artificial sweat at pH 5.5 and 8.0. Approximately the same amount of silver was released from differently modified cotton fabrics in artificial sweat. Larger amount of silver was released in the sweat at pH 8.0.

scholarly journals Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

2019 ◽  
Vol 10 (2) ◽  
pp. 98-102
Author(s):  
Akmel Suhada
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Endemic Plant ◽  
Dpph Method ◽  
Hexane Extracts

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity  was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibition zone 10 mm at  concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

scholarly journals Comparative Study on Antibacterial Activity of Crude and Silver Nanoparticles Synthesized Leaf Extract of Carica papaya against Chosen Isolates

2021 ◽  
Vol 70 (1) ◽  
Author(s):  
Pandiarajan. P ◽  
Andrew Pradeep ◽  
Januja J ◽  
Dhurairaj Satheesh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Carica Papaya ◽  
Proteolytic Enzymes ◽  
Antibiotic Resistance Genes ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Antimicrobial Drugs ◽  
Ft Ir ◽  
Phytochemical Components

The Emergence of Human Pathogens with Antibiotic resistance genes has shifted the focus towards the plant origin antimicrobial drugs with advanced nano-based methods. Screening of phytochemical components is done using various biochemical methods. UVvisible spectrophotometer is utilized to determine the silver Nanoparticle's size, shape, and its stable nature in the aqueous colloidal solution. FT-IR spectral analysis to identify the bio molecules responsible for the reduction of Ag+ ions. The extracts of Carica papaya leaves were found to be rich in Vitamins, Phenols, Proteolytic enzymes which acts as excellent Antimicrobial agent. Antibacterial activity of crude extracts and silver Nanoparticles synthesized from Carica papaya leaves was evaluated against chosen clinical isolates, which shows the effective zone of inhibition lesser side effects. Though crude extract has shown significant results, silver nanoparticles synthesized using specific key phytochemical component would have the better inhibiting capacity.

scholarly journals Biomimetic Synthesis of Silver Nanoparticles from Aqueous Extract of Saraca indica and its Profound Antibacterial Activity

2020 ◽  
Vol 11 (1) ◽  
pp. 8110-8120
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Silver Nanoparticle ◽  
Pathogenic Bacteria ◽  
Drug Formulation ◽  
Biomimetic Synthesis ◽  
Visible Absorption ◽  
The Stability ◽  
Average Size

The present findings were focused on green synthesis of silver nanoparticles through an aqueous extract of Saraca indica. The stability of the nanoparticle was achieved through the optimization of physico-chemical parameters. The sharp UV-visible absorption maximum at 400 was observed for biological synthesized silver nanoparticles. The spectroscopic analysis was thus used to assess the formation of silver nanoparticles. The AFM analysis did analyze the morphology of the nanocomposite, which was further confirmed through TEM micrograph. The electron micrograph image discloses that silver nanoparticles were polydispersed and dominantly as spherical with size ranges from 40nm to 100nm. The average size distribution was 49nm. The chemical reductions of Ag+ ions were further confirmed through FTIR. The biogenic silver nanoparticle and their drug formulation showed profound antibacterial activity against pathogenic bacteria. The flavonoids rich binding of silver nanoparticle showed great medicinal potential and can be used for the treatment of several harmful infectious diseases. Hence, plant-based metal nanoparticles meet the demand for less toxic formulation during drug development and its delivery.

scholarly journals Biosynthesis of Silver Nanoparticles by Aspergillus sakultaensis and Its Antibacterial Activity against Human Pathogens

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Marwa Abdel-Kareem ◽  
Abdel-Naser Zohri ◽  
Abdel-Hamied Rasmey
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Human Pathogens
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