scholarly journals Comparison of Phytochemical Profile, Mineral Content, and In Vitro Antioxidant Activities of Corchorus capsularis and Corchorus olitorius Leaf Extracts from Different Populations

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Ashok Biswas ◽  
Susmita Dey ◽  
Defang Li ◽  
Yi Liu ◽  
Jiangjiang Zhang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Hierarchical Cluster ◽  
Bioactive Compound ◽  
Corchorus Olitorius ◽  
Leaf Extracts ◽  
Medicinal Uses ◽  
Corchorus Capsularis ◽  
Saponin Content

In addition to fiber, Corchorus (jute) leaf is also rich in secondary metabolites and is used in folk medicine in jute-producing communities. It has been reported that jute halts progression of and helps manage different chronic diseases like tumors, obesity, diabetes, and cardiovascular diseases. Various phytochemical, mineral, and antioxidant potency properties of 30 genotypes belonging to Corchorus capsularis and Corchorus olitorius were evaluated in the current study. The results demonstrate that the range of total flavonoids and polyphenols was 3.04 to 13.66 mg rutin equivalent (RE)/g and 5.12 to 7.78 mg gallic acid equivalent (GAE)/g DW, respectively. Total tannin and saponin content in both species was 13.08 to 26.95 mg tannic acid (TAE)/g and 34.45 to 114.59 mg tea saponin (TSE)/g DW, respectively, when analyzed for the first time. Moreover, this study sincerely establishes that jute leaf is a great source of mineral elements (magnesium, zinc, and selenium) and could also be a good energy source. The antioxidant properties of samples were examined with three unique strategies, including DPPH, FRAP, and ABTS values of leaf extract ranging from 206.42 to 351.77 μg/ml (IC50), 16.69 to 94.69 mmol Fe (II)/g DW, and 50.27 to 149.90 mmol trolox equivalent (TE)/g DW, respectively. A principle component analysis (PCA) explained 58.52% of the variance, while a hierarchical cluster analysis (HCA) was performed to construct five distinct groups based on their secondary compound metabolites content and antioxidant activities. Therefore, this study facilitates selection of the high genotypes that might be used as new materials for developing industrial and medicinal uses in addition to sorting genotypes for future genetic engineering purposes in order to enhance a particular bioactive compound and its natural antioxidants that are beneficial for human health.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

Evaluation of the Potential Cardioprotective Activity of Some Saudi Plants against Doxorubicin Toxicity

2012 ◽  
Vol 67 (5-6) ◽  
pp. 297-307 ◽  
Author(s):  
Osama M. Ashour ◽  
Ashraf B. Abdel-Naim ◽  
Hossam M. Abdallah ◽  
Ayman A. Nagy ◽  
Ahmed M. Mohamadin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Serum Markers ◽  
Protective Effects ◽  
Medicinal Uses ◽  
Cordia Myxa

Doxorubicin (DOX) is an anthracycline antibiotic widely used as a chemotherapeutic agent in the treatment of several tumours. However, its cardiac toxicity limits its use at maximum therapeutic doses. Most studies implicated increased oxidative stress as the major determinant of DOX cardiotoxicity. The local Saudi flora is very rich in a variety of plants of quite known folkloric or traditional medicinal uses. Tribulus macropterus Boiss., Olea europaea L. subsp. africana (Mill.) P. S. Green, Tamarix aphylla (L.) H. Karst., Cynomorium coccineum L., Cordia myxa L., Calligonum comosum L’ Hér, and Withania somnifera (L.) Dunal are Saudi plants known to have antioxidant activities. The aim of the current study was to explore the potential protective effects of methanolic extracts of these seven Saudi plants against DOX-induced cardiotoxicity in rats. Two plants showed promising cardioprotective potential in the order Calligonum comosum > Cordia myxa. The two plant extracts showed potent in vitro radical scavenging and antioxidant properties. They significantly protected against DOX-induced alterations in cardiac oxidative stress markers (GSH and MDA) and cardiac serum markers (CK-MB and LDH activities). Additionally, histopathological examination indicated a protection against DOX-induced cardiotoxicity. In conclusion, C. comosum and C. myxa exerted protective activity against DOX-induced cardiotoxicity, which is, at least partly, due to their antioxidant effect

scholarly journals TLC Profiling of Leaves Extracts of Some Aloe Threatened Species Endemic to Madagascar for Their Antioxidant Activity

2020 ◽  
Vol 2 (3) ◽  
pp. 653-662
Author(s):  
Rokiman Letsara ◽  
Rigobert Andrianantenaina ◽  
Gédéon Ngiala Bongo ◽  
Colette Masengo Ashande ◽  
Mahendra Ilmi S Matondang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
World Health ◽  
Leaf Extracts ◽  
Phytochemical Compounds ◽  
Vitro Model ◽  
Health Organization

The World Health Organization reported that at least 80% of populations rely on traditional medicine and medicinal plants for their primary health care. Due to their phytochemical compounds, the plants of the Aloe genus are reported to have high potential antiCovid-19 (and antioxidant properties. The aim of this study is to evaluate the in vitro antioxidant activity of some Malagasy endangered species of Aloe genus. The ethanolic extract of few Aloe of Madagascar leaf extracts was fractionated by liquid-liquid partition using hexane. In total 18 different fractions from 9 species have been used to determine their antioxidant activity through in vitro model by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. Both hexanic extract and aqueous extract displayed antioxidant activities in four species. The most evident antioxidant activity was expressed by A. helenae.

scholarly journals Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Musbau Adewunmi Akanji ◽  
Samson Olasunkanmi Olukolu ◽  
Mutiu Idowu Kazeem
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Leaf Extracts ◽  
Chemically Induced ◽  
Radical Scavenging Ability ◽  
Aerva Lanata ◽  
Radical Scavenging Properties

The leaves of Aerva lanata are one of the indigenous medicinal plants used in the management of diabetes mellitus and its associated complications in Africa. However, its effect on the activities of diabetes-related enzymes has not been investigated. This study evaluated the in vitro inhibitory effects of different extracts of the A. lanata leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. Aqueous, ethanol, and hydroethanol extracts of A. lanata leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The results obtained showed that the hydroethanol extract of the A. lanata leaf optimally inhibited both α-amylase (IC50: 2.42 mg/mL) and α-glucosidase (IC50: 0.23 mg/mL). The Lineweaver-Burk plot revealed that the mode of inhibition of both enzymes by the hydroethanol extract was uncompetitive. However, the hydroethanol and aqueous extracts displayed the best DPPH and ABTS radical-scavenging ability, respectively. It can be concluded that the A. lanata extract inhibited the activities of both α-amylase and α-glucosidase uncompetitively, which may be attributed to its free radical-scavenging properties and rich phenolic composition.

scholarly journals Potential In Vitro and In Vivo Antioxidant Activities from Piper Crocatum and Persea Americana Leaf Extracts

2019 ◽  
Vol 12 (2) ◽  
pp. 661-667
Author(s):  
Fatmawaty Fatmawaty ◽  
Ni G. M. Anggreni ◽  
Naufal Fadhil ◽  
Vivitri D. Prasasty
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Persea Americana ◽  
Health Claims ◽  
In Vitro Assays ◽  
Moderate Activity ◽  
Leaf Extracts ◽  
Water Fraction

Piper crocatum and Persea americana Mill leaves are commonly used in traditional medicinal remedies, such as antidiabetes, antitumors and Alzheimer treatment. However, the extensive use of plant extracts over worldwide becomes significant concerns including its safety, efficacy, and quality. Therefore, the accurate scientific evaluation has become a precondition for acceptance of herbal health claims. The aim of this study was to determine the antioxidant activities of Piper crocatum Ruiz & Pav and Persea americana Mill leaf extracts. Isolation of antioxidant fractions were conducted using organic solvent extraction techniques. Antioxidant assays were conducted by using in vitro and in vivo methods involving DPPH and MDA methods. In this study, in vitro assays of Persea americana Mill and Piper crocatum Ruiz & Pav leaf extracts showed the best activity in water fraction. Moreover, in vivo assays of both plant leaf extracts showed the best dose at 8 mg. Persea americana Mill and Piper crocatum Ruiz & Pav leaf extracts have been successfully determined in antioxidant actions in vitro and in vivo. Persea americana Mill in water and ethanol solvents exhibit strong antioxidant properties. Meanwile, Piper crocatum Ruiz & Pav exhibit moderate activity in water and weak antioxidant activity in ethanol. Both plant leaves showed that 8 mg dose was better than the dose of 4 mg and 16 mg in vivo.

scholarly journals Chemical Profile and Antioxidant Activity of the Oil from Peony Seeds (Paeonia suffruticosa Andr.)

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Xin Yang ◽  
Di Zhang ◽  
Li-min Song ◽  
Qian Xu ◽  
Hong Li ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Virgin Olive Oil ◽  
Antioxidant Properties ◽  
Extra Virgin Olive Oil ◽  
Chemical Profile ◽  
Paeonia Suffruticosa ◽  
Oxidative Damages ◽  
Development And Utilization ◽  
Key Indicators

Peony seed oil (PSO) is a novel vegetable oil developed from the seeds of Paeonia suffruticosa Andr. The present study aimed to make an overall investigation on the chemical profile and antioxidant activities of PSO for reasonable development and utilization of this new resource food. Chemical analysis revealed that PSO was characterized by an uncommon high portion of α-linolenic acid (>38%), fairly low ratio of n-6 to n-3 polyunsaturated fatty acids (0.69), and much higher content of γ-tocopherol than various conventional seed oils. In vitro assay indicated that PSO is a more potent scavenger of free radicals than extra virgin olive oil. Moderate intake of PSO exhibited obvious protection against various oxidative damages such as tetrachloromethane-induced acute liver injury in mice and diet-induced hyperlipidemia in rats. The changes in the key indicators of oxidative injury and fatty acid composition in the liver caused by PSO administration were measured, and the results demonstrated that antioxidant properties of PSO are closely related to their characteristic chemical composition. Consequently, the present study provided new evidence for the health implications of PSO, which deserves further development for medical and nutritional use against oxidative damages that are associated with various diseases.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Synthesis, Neuroprotection, and Antioxidant Activity of 1,1′-Biphenylnitrones as α-Phenyl-N-tert-butylnitrone Analogues in In Vitro Ischemia Models

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1127 ◽  
Author(s):  
Beatriz Chamorro ◽  
David García-Vieira ◽  
Daniel Diez-Iriepa ◽  
Estíbaliz Garagarza ◽  
Mourad Chioua ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Human Neuroblastoma ◽  
In Vitro Ischemia ◽  
Lipoxygenase Inhibition ◽  
Antioxidant Agent

Herein, we report the neuroprotective and antioxidant activity of 1,1′-biphenyl nitrones (BPNs) 1–5 as α-phenyl-N-tert-butylnitrone analogues prepared from commercially available [1,1′-biphenyl]-4-carbaldehyde and [1,1′-biphenyl]-4,4′-dicarbaldehyde. The neuroprotection of BPNs1-5 has been measured against oligomycin A/rotenone and in an oxygen–glucose deprivation in vitro ischemia model in human neuroblastoma SH-SY5Y cells. Our results indicate that BPNs 1–5 have better neuroprotective and antioxidant properties than α-phenyl-N-tert-butylnitrone (PBN), and they are quite similar to N-acetyl-L-cysteine (NAC), which is a well-known antioxidant agent. Among the nitrones studied, homo-bis-nitrone BPHBN5, bearing two N-tert-Bu radicals at the nitrone motif, has the best neuroprotective capacity (EC50 = 13.16 ± 1.65 and 25.5 ± 3.93 μM, against the reduction in metabolic activity induced by respiratory chain blockers and oxygen–glucose deprivation in an in vitro ischemia model, respectively) as well as anti-necrotic, anti-apoptotic, and antioxidant activities (EC50 = 11.2 ± 3.94 μM), which were measured by its capacity to reduce superoxide production in human neuroblastoma SH-SY5Y cell cultures, followed by mononitrone BPMN3, with one N-Bn radical, and BPMN2, with only one N-tert-Bu substituent. The antioxidant activity of BPNs1-5 has also been analyzed for their capacity to scavenge hydroxyl free radicals (82% at 100 μM), lipoxygenase inhibition, and the inhibition of lipid peroxidation (68% at 100 μM). Results showed that although the number of nitrone groups improves the neuroprotection profile of these BPNs, the final effect is also dependent on the substitutent that is being incorporated. Thus, BPNs bearing N-tert-Bu and N-Bn groups show better neuroprotective and antioxidant properties than those substituted with Me. All these results led us to propose homo-bis-nitrone BPHBN5 as the most balanced and interesting nitrone based on its neuroprotective capacity in different neuronal models of oxidative stress and in vitro ischemia as well as its antioxidant activity.

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker
Keyword(s):  
Ascorbic Acid ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

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