scholarly journals Phytochemical Analysis, Anti-inflammatory, and Antioxidant Activities of Dendropanax dentiger Roots

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Li Yang ◽  
Yiwei Fang ◽  
Ronghua Liu ◽  
Junwei He
Keyword(s):  
High Performance ◽  
Cyclic Peptide ◽  
Antioxidant Activities ◽  
Inflammatory Diseases ◽  
Phytochemical Analysis ◽  
Chemical Compositions ◽  
Biological Functions ◽  
Cox 2 ◽  
Anti Inflammatory

Dendropanax dentiger root is a traditional medicinal plant in China and used to treat inflammatory diseases for centuries, but its phytochemical profiling and biological functions are still unknown. Thus, a rapid, efficient, and precise method based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied to rapidly analyse the phytochemical profiling of D. dentiger with anti-inflammatory and antioxidant activities in vitro. As a result, a total of 78 chemical compositions, including 15 phenylpropanoids, 15 alkaloids, 14 flavonoids, 14 fatty acids, 7 phenols, 4 steroids, 4 cyclic peptides, 3 terpenoids, and 2 others, were identified or tentatively characterized in the roots of D. dentiger. Moreover, alkaloid and cyclic peptide were reported from D. dentiger for the first time. In addition, the ethanol crude extract of D. dentiger roots exhibited remarkable anti-inflammatory activity against cyclooxygenase- (COX-) 2 inhibitory and antioxidant activities in vitro. This study is the first to explore the phytochemical analysis and COX-2 inhibitory activity of D. dentiger. This study can provide important phytochemical profiles and biological functions for the application of D. dentiger roots as a new source of natural COX-2 inhibitors and antioxidants in pharmaceutical industry.

scholarly journals Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Reina M. Toro ◽  
Diana M. Aragón ◽  
Luis F. Ospina ◽  
Freddy A. Ramos ◽  
Leonardo Castellanos
Keyword(s):  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Physalis Peruviana ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Bio Assay

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

2,5-disubstituted-4-thiazolidinones: Synthesis, anti-inflammatory, free radical scavenging potentials and structural insights through molecular docking

2021 ◽  
Vol 18 ◽  
Author(s):  
Jagseer Singh ◽  
Pooja A Chawla ◽  
Rohit Bhatia ◽  
Shamsher Singh
Keyword(s):  
Free Radicals ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Assay Method ◽  
Acidic Group ◽  
Active Compounds ◽  
Cox 2 ◽  
Anti Inflammatory

: The present work reports synthesis and screening of fifteen 2,5-disubstituted-4-thiazolidinones with different substitutions of varied arylidene groups at imino. The structures of the compounds were confirmed by spectral characterization. The compounds were subjected to in vivo anti-inflammatory and in vitro antioxidant activities. The derivatives possessed remarkable activities quite close to standard drugs used. Unlike conventional non-selective NSAIDs, the synthesized compounds did not contain any acidic group, thereby ensuring a complete cure from ulcers. To further substantiate the claim for safer derivatives, the active compounds were docked against the cyclooxygenase (COX)-2 enzyme. It was found that 4-fluorophenylimino substituent at 2- position and 3-nitro moiety on a 5-benzylidene nucleus of the 4-thiazolidinone derivative fitted in the COX-2 binding pocket. The compounds exhibited remarkable activity in scavenging free radicals, as depicted by the DPPH assay method. The structure-activity relationship was also established in the present work with respect to the nature and position of the substituents. The active compounds were evaluated for drug-like nature under Lipinski’s rule of five, and the toxicity behaviour of active compounds was predicted using ADMETlab software. The compounds have the potential to target degenerative disorders associated with inflammation and the generation of free radicals.

Phytochemical Analysis and In-vitro Anti-Diabetic and Anti- Inflammatory study of root extract of Apama siliquosa LAMK.

2021 ◽  
pp. 5838-5842
Author(s):  
Manu Jose ◽  
Stephin Baby ◽  
Dona Mathew ◽  
Naurin Muhammed ◽  
Jayalakshmi P M
Keyword(s):  
Inflammatory Diseases ◽  
Herbal Medicines ◽  
Alpha Amylase ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Inhibition Assay ◽  
Ic50 Value ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

The demand for herbal medicines in many pharmaceutical sectors is growing at a drastic rate due to their improved pharmacological actions, minimal side effects and cost-effectiveness. Anti-inflammatory phytochemicals are found to be efficacious against the treatment of inflammatory diseases like rheumatoid arthritis, glomerulonephritis, hepatitis, inflammatory bowel disease, etc. Anti-diabetic phytochemicals are found to treat the increasing incidence of diabetes prevalent globally. This work aims to perform the phytochemical screening and to evaluate the antidiabetic and anti-inflammatory activity of crude extract of Apama siliquosa Lamk. The method employed for obtaining the active principles includes soxhlation technique with methanol as solvent. The anti-inflammatory property was studied in vitro using inhibition of albumin denaturation technique as well as heat-induced hemolysis and IC50 value was found to be 39.5μg/ml and 36.30μg/ml respectively. The anti-diabetic activity was estimated using the alpha-amylase inhibition assay and Glucose diffusion inhibitory study. The IC50 value for alpha-amylase inhibition assay was found to be 15.75μg/ml. It also shows a strong inhibition of glucose across the dialysis membrane.

scholarly journals Study on the Bioassay of Anti-Inflammatory Effects of Fuke Qianjin Capsule Based on COX-2 Inhibiting Activity

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Run-ran Ma ◽  
Xiao-juan Yang ◽  
Ying Huang ◽  
Shuai-shuai Chen ◽  
Xiao-he Xiao ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Parallel Line ◽  
Multiple Linear Regression Analysis ◽  
Positive Control ◽  
Bioassay Method ◽  
Cox 2 ◽  
Anti Inflammatory

Fuke Qianjin Capsule (FKQJ) is a common TCM compound formula in the treatment of gynecological inflammation-related diseases. This study intends to explore and establish a bioassay method to further improve its quality control. The bioassay method for the determination of anti-inflammatory biopotency was established based on its inhibitory activity on recombinant human cyclooxygenase-2 (COX-2), an active target of FKQJ in the treatment of female pelvic inflammatory disease. We firstly established chemical fingerprint of 20 batches of FKQJ by ultra-high-performance liquid chromatography to identify the components and analyze the chemical similarities. The similarity within different batches of FKQJ was relatively high. The values of similarity of the 19 batches were between 0.973 and 0.995, while one batch’s similarity value was 0.813. Celecoxib, a selective inhibitor of COX-2, was chosen as the positive control drug in COX-2 activity assay to establish an anti-inflammatory biopotency detection method based on parallel line test of qualitative response. The methodological investigation showed that the method possessed good repeatability and precision. Secondly, the anti-inflammatory biopotency of 20 batches of FKQJ for inhibiting COX-2 was determined. The results showed that the biopotency of different batches of FKQJ ranged from 676 U/μg to 1310 U/μg, with average value of 918 U/μg and RSD of 16.7%. Based on multiple linear regression analysis, we found that three contents were highly correlated with the anti-inflammatory biopotency, while chlorogenic acid was validated of the strongest anti-inflammatory activity in vitro. Compared with chemical detection, bioassay can better reflect the quality fluctuation of different batches of products and correlate the known pharmacodynamic targets. The supplement of the bioassay method based on chemical evaluation is helpful to improve the quality control ability of Chinese patent medicine and ensure its clinical efficacy is stable and controllable.

Phytochemical analysis and In vitro screening of putative dual COX-2/5-LOX inhibitors from Cucumis ficifolius root as an Anti-inflammatory agent

2019 ◽  
Vol 12 (11) ◽  
pp. 5240
Author(s):  
Tesfay Weletnsae ◽  
Kidu Hintsa ◽  
Tekleweyni Tadege ◽  
V. K. Gopalakrishnan ◽  
Zenebe Hagos ◽  
...  
Keyword(s):  
Phytochemical Analysis ◽  
In Vitro Screening ◽  
Inflammatory Agent ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Lox Inhibitors

scholarly journals Anti-Inflammatory Activities of Leaf Oil from Cinnamomum subavenium In Vitro and In Vivo

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Xincai Hao ◽  
Weiguang Sun ◽  
Changbin Ke ◽  
Fuqian Wang ◽  
Yongbo Xue ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Scientific Basis ◽  
Paw Edema ◽  
Leaf Oil ◽  
Cox 2 ◽  
Anti Inflammatory

The study determined the chemical constituents and anti-inflammatory effects of leaf oil from Cinnamomum subavenium (CS-LO) that has been used in folk medicine to treat various symptoms including inflammation. The anti-inflammatory effects of the oil were evaluated by LPS-stimulated RAW264.7 cells and the Carr-induced hind mouse paw edema model, respectively. In vitro, nitric oxide (NO), prostaglandin E2 (PGE2), TNF-α, IL-6, and IL-1β were significantly decreased by CS-LO, and the expression of nuclear factor-κB (NF-κB) protein was blocked as well. In in vivo, the malondialdehyde (MDA) and paw edema levels were decreased by CS-LO, and the same result came up on the NO and tumor necrosis factor (TNF-a) of serum at the 5th h after Carr injection. In addition, iNOS and COX-2 immunoreactive cells of the paw tissue were decreased significantly by CS-LO (200 mg/kg) in histological examination. The present findings indicated that CS-LO have anti-inflammatory properties, and the effects might be caused through inhibiting iNOS, COX-2, TNF-α, IL-1β, and IL-6 expression via affecting NF-κB pathway, which will provide a power scientific basis for CS-LO to be used as the treatment of inflammatory diseases.

scholarly journals In Vivo Anti-Inflammatory and In Vitro Antioxidant Activities of Moroccan Medicinal Plants

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601
Author(s):  
Mina Moussaid ◽  
Abd Elaziz Elamrani ◽  
Nourdinne Bourhim ◽  
Mohamed Benaissa
Keyword(s):  
Antioxidant Activities ◽  
Inflammatory Diseases ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Wild Plants ◽  
Acid Oxidation ◽  
Mentha Pulegium ◽  
Anti Inflammatory

Five ethanol extracts of wild plants from the Casablanca region (Morocco) used in local traditional medicine for the treatment of inflammatory diseases were evaluated for their in vivo topical anti-inflammatory activity (inhibition of carrageenan-induced ear edema in mice) and in vitro antioxidant and antiradical properties (inhibition of linoleic acid oxidation, DPPH radical scavenging). All the extracts showed an anti-inflammatory effect: 300 μg/cm2 provoked edema reductions ranging from 22 to 28%. All the extracts also exerted radical scavenging and/or antioxidant properties, the most active plant being Mentha pulegium L. (Lamiaceae), which contained the highest amount of phenolics (339 mg/g), and flavonoids (16.7 mg/g).

scholarly journals Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

2021 ◽  
Vol 22 (16) ◽  
pp. 8716
Author(s):  
Hun Hwan Kim ◽  
Sang Eun Ha ◽  
Preethi Vetrivel ◽  
Pritam Bhagwan Bhosale ◽  
Seong Min Kim ◽  
...  
Keyword(s):  
Binding Affinity ◽  
High Performance ◽  
Kynurenic Acid ◽  
Screening Methods ◽  
Bioactive Ingredients ◽  
Pharmaceutical Industries ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The antioxidant and anti-inflammatory potentials of polyphenols contained in Gynura procumbens (GP) extract were systematically analyzed. Polyphenols in GP were analyzed for nine peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS), and quantitatively determined through each standard. A total of nine polyphenolic compounds were identified in the samples and their MS data were tabulated. To determine the potential of bioactive ingredients targeting DPPH and COX-2, we analyzed them by ultrafiltration combined with LC. The results identified the major compounds exhibiting binding affinity for DPPH and COX-2. Caffeic acid, kynurenic acid, and chlorogenic acid showed excellent binding affinity to DPPH and COX-2, suggesting that they can be considered as major active compounds. Additionally, the anti-inflammatory effect of GP was confirmed in vitro. This study will not only be used to provide basic data for the application of GP to the food and pharmaceutical industries, but will also provide information on effective screening methods for other medicinal plants.

scholarly journals Antimalarial, Antioxidant Activities and Chemoprofile of Sansevieria liberica Gerome and Labroy (Agavaceae) Leaf Extract

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ifeoma Celestina Orabueze ◽  
Sarah Chiamaka Uzor ◽  
Bou Ndiaye ◽  
Desire Uba ◽  
Duncan Azubuike. Ota ◽  
...  
Keyword(s):  
Vitamin C ◽  
High Performance ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Reducing Power ◽  
In Vitro Models ◽  
Phytochemical Analysis ◽  
Perennial Plant

Background. Sansevieria liberica Gerome and Labroy (Agavaceae) is a religious and ornamental perennial plant with highly valued medicinal usage in Nigeria. Sansevieria liberica is used in the management of malarial fever. The ease of development of resistance to available antimalarial drugs has resulted in increased clinical failure and mortality. The study investigated the antimalarial effects of Sansevieria liberica (SL) leaf extract in mice infected with Plasmodium berghei. Materials and Methods. The ability of SL leaf extract to suppress the growth of malaria parasites in early (suppressive) and established (curative) infections was established using animal models. The mean survival time (MST) was determined. The antioxidant potential was established using two standard in vitro models. High-performance liquid chromatography (HPLC) and phytochemical analysis methods were used to create a chemoprofile fingerprint of SL hydroethanolic leaf extract. Results. At 200, 400, and 400·mg kg−1, SL produced 68.71, 70.74, and 75.09% parasite suppression in the suppressive model while the curative model gave a percentage of cure of 71.09, 72.60, and 62.09, respectively. The animals lived longer compared to both negative and positive controls but were not fully protected. The IC50 values of SL and vitamin C were calculated to be 3.599 µg mL−1 and 3.08 µg mL−1, respectively. The reducing power of vitamin C was significantly ( P < 0.05 ) higher than that of SL extract. Some flavonoids were established as possible marker compounds for SL leaf extract. Discussion and Conclusions. The antimalarial assay results demonstrated that the use of SL in folk medicine may have scientific support.

scholarly journals Antioxidant and anti-inflammatory activities of loquat (Eriobotrya japonica) tea

2013 ◽  
Vol 3 (11) ◽  
pp. 447 ◽  
Author(s):  
Phyu Phyu Khine Zar ◽  
Kozue Sakao ◽  
Fumio Hashimoto ◽  
Akiko Morishita ◽  
Makoto Fujii ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Chemical Characterization ◽  
Eriobotrya Japonica ◽  
Raw 264.7 Cells ◽  
Phenolic Components ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Tea Extract ◽  
Chemical Quantification

Background: Fresh loquat leaves contain several kinds of flavonoids and have been reported to have preventive effects against some human diseases such as diabetes, coughs and ulcers,. Recently, fresh loquat leaves in Japan were processed to a beverage, called loquat tea, after the fresh leaves are roasted at 350C for 30 minutes. However, the scientific evidence supporting the functions of these processed leaves is still minimal.Objective: The aim of this study is to investigate the antioxidant and anti-inflammatory activities of roasted loquat tea extract (LTE) in vitro and in culture cells.Methods: Bioactive fractions of LTE were separated by column chromatograph. Antioxidant activities were determined by DPPH and ROS assay. Pro-inflammatory mediators cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) were determined by Western blot and ELISA assay, respectively. Chemical quantification and characterization were analyzed by HPLC, FR-IR, and NMR. Phenolic content was measured by Folin-Ciocalteu assay.Results: The results showed that loquat tea extract (LTE) possessed stronger DPPH scavenging activity than fresh. Cellular data revealed that LTE inhibited the production of reactive oxygen species (ROS), and further suppressed the production of COX-2 and PGE2 in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Chemical quantification and characterization data indicated that LTE contained new bioactive phenolic components that were produced from the roasting processes of fresh loquat leaves.Conclusions: Loquat tea made from roasted loquat leaves contained new bioactive phenolic compounds that contribute to its antioxidant and anti-inflammatory activities.Keywords: Loquat tea, Antioxidant activity, Anti-inflammatory activity, Chemical characterization

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