scholarly journals In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of Senecio glaucus from Saudi Arabia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Ali S. Alqahtani ◽  
Rashed N. Herqash ◽  
Omar M. Noman ◽  
Fahd A. Nasr ◽  
Nouf Alyhya ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Side Effects ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Hplc Method ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Chloroform Fraction ◽  
Promising Source

Current treatments for complex diseases have remarkable side effects that negatively impact patients’ quality of life. Thus, natural compounds with fewer side effects represent a promising source for safe drugs. The genus Senecio is widely used in folk medicine due to its various pharmacological properties. In the present study, the total phenolic content of Senecio glaucus, which is grown in Saudi Arabia, was assessed using the Folin-Ciocalteau colorimetric method. Scavenging DPPH and ABTS assays were utilized to determine the antioxidant properties of S. glaucus fractions, and MTT assay was used to screen the cytotoxic activity of S. glaucus against various cancer cells. In addition, HPLC-UV was utilized to detect the presence of two phenolic acids, namely, vanillic acid (VA) and gallic acid (GA). Among all fractions tested, S. glaucus chloroform fraction (SGCF) yielded the highest value (125.3 mg·GA/g) in terms of total phenolic content. SGCF also exhibited the highest scavenging activities (76.7 and 74.1%) on both DPPH and ABTS assays, respectively. Similarly, SGCF also possessed the most potent cytotoxic activity against the MCF-7 cell line, with an IC50 value of 41.8 μg/ml. The validated HPLC method confirmed the presence of VA (4.8 μg/mg DW) and GA (3.9 μg/mg DW) in SGCF. Overall, our data show that S. glaucus had antioxidant and cytotoxic properties. A developed validated HPLC method which could be helpful for quantifying phenolic compounds in S. glaucus was established.

scholarly journals Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of Dipterocarpus alatus

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3083 ◽  
Author(s):  
Chawalit Yongram ◽  
Bunleu Sungthong ◽  
Ploenthip Puthongking ◽  
Natthida Weerapreeyakul
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Composition Analysis ◽  
Cytotoxic Activities ◽  
Antioxidant Power ◽  
Dipterocarpus Alatus

Dipterocarpus alatus (Dipterocarpaceae) is a medicinal plant whose use is well known for the treatment of genito-urinary diseases. However, there is no report of its cytotoxic potential. In this study, the chemical composition, antioxidant and cytotoxic activities of extracts of the leaves, bark, twigs and oleo-resin from D. alatus are investigated. Cytotoxicity was measured by the neutral red (NR) assay against HCT116, SKLU1, SK-MEL2, SiHa and U937 cancer cell lines and antioxidant capacity was evaluated by DPPH, ABTS radical scavenging, and ferric reducing antioxidant power (FRAP) assays. The chemical composition was analyzed by gas chromatography–mass spectrometry (GC-MS). Leaf, bark and twig extracts exhibited stronger antioxidant activity than oleo-resin, with bark extract showing the highest antioxidant activity and the highest total phenolic content. All samples showed more cytotoxic activity against the U937 cell line than HCT116, SKLU1, SK-MEL2 and SiHa cells with oleo-resin being more cytotoxic than melphalan against U937 cells. Chemical composition analysis of oleo-resin by GC-MS showed that the major components were sesquiterpenes, namely α-gurjunene (30.31%), (-)-isoledene (13.69%), alloaromadendrene (3.28%), β-caryophyllene (3.14%), γ-gurjunene (3.14%) and spathulenol (1.11%). The cytotoxic activity of oleo-resin can be attributed to the sesquiterpene content, whereas the cytotoxic and antioxidant activities of leaf, bark and twig extracts correlated to total phenolic content.

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

Phytochemical screening, total phenolic content, antioxidant, and cytotoxic activity of five peruvian plants on human tumor cell lines

2018 ◽  
Vol 10 (2) ◽  
pp. 161 ◽  
Author(s):  
Oscar Herrera-Calderon ◽  
Corina Alvarado-Puray ◽  
JorgeLuis Arroyo-Acevedo ◽  
JuanPedro Rojas-Armas ◽  
Victor Chumpitaz-Cerrate ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Human Tumor ◽  
Total Phenolic ◽  
Tumor Cell Lines ◽  
Phytochemical Screening ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines

scholarly journals Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content

FEBS Open Bio ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 86-95 ◽  
Author(s):  
Malgorzata Kozyra ◽  
Agnieszka Korga ◽  
Marta Ostrowska ◽  
Ewelina Humeniuk ◽  
Grzegorz Adamczuk ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Melanoma Cells ◽  
Total Phenolic

scholarly journals Comparative Study of Phytochemical, Antioxidant, and Cytotoxic Activities and Phenolic Content of Syzygium aqueum (Burm. f. Alston f.) Extracts Growing in West Sumatera Indonesia

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Afrizal Itam ◽  
Mutia Siska Wati ◽  
Vina Agustin ◽  
Nursal Sabri ◽  
Rafika Aris Jumanah ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Stem Bark ◽  
Natural Antioxidant ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Hexane Extracts ◽  
Phytochemical Constituents ◽  
Ethyl Acetate Extracts

Syzygium aqueum, consisting of various fruit colors, is one of the plants that have been used as traditional medicine. This study aims to evaluate and compare phytochemical, antioxidant, and cytotoxic activities and total phenolic content of leaves and stem bark extracts of S. aqueum with pink and red fruits, in order to identify the best extract that can be used as a natural antioxidant. Phytochemical constituents were evaluated qualitatively using chemicals, while cytotoxic activities were identified using the brine shrimp lethality test. Total phenolic content was determined via the Folin–Ciocalteu method. Leaves and stem bark of S. aqueum contained flavonoids, phenolics, and triterpenoids, but the stem bark also contained saponins and alkaloids. Methanol and ethyl acetate extracts of leaves and stem bark were categorized as very powerful antioxidants to DPPH (IC50 9.71–38.69 μg/mL) and hydrogen peroxide (IC50 16.44–44.02 μg/mL), while hexane extracts were inactive. Methanol, ethyl acetate, and hexane extracts of leaves and stem bark were categorized as moderately cytotoxic to A. salina larvae (LC50 104.04–440.65 μg/mL). Comparing leaves and stem barks, antioxidant and cytotoxic activities of stem bark extracts were higher than those of leaves extracts. Total phenolic content of leaves extracts was higher than that of stem bark extracts where the order of total phenolic content progressed from methanol extracts > ethyl acetate extracts > hexane extracts. Therefore, the stem bark of S. aqueum was identified as the better source of natural antioxidant compared with the leaves.

Phenolic Composition and Antioxidant Activity of Leaves of Strawberry Tree (Arbutus unedo L.) Populations from Morocco

2021 ◽  
Author(s):  
Z. Ait lhaj ◽  
K. Taghzouti ◽  
A. Bouyahya ◽  
G. Diria ◽  
K. Bakhy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Scavenging Effect ◽  
Promising Source

This study was aimed at determining the phenolic composition and the antioxidant activity of leaves of Arbutus unedo populations belonging to seven Moroccan locations. Antioxidant activity is evaluated using two methods: scavenging effect on DPPH and scavenging effect on ABTS radicals. The Arbutus unedo leaf extracts displayed average values of 61.95 mg GAE, 3.60 mg QE, and 0.15 mg CE per g of dry weight (DW) in polyphenols, flavonoids, and tannins contents, respectively. All extracts exhibited a strong antioxidant effect on both assays, presenting mean IC50 values of 4.22 in DPPH assay and 4.86 μg/ml in ABTS assay. Significant variability (P < 0.0001) was showed among surveyed populations; samples from the Chefchaouen location (CH) was revealed the highest total phenolic content (107.67 mg GAE/g DW) and the strongest antioxidant activity (IC50 values of 0.80 and 1.56 μg/ml, in DPPH and ABTS tests, respectively). The phenolic profile achieved by HPLC-DAD-ESI/MS was dominated by gallic acid, Gallic acid glucose derivative, and Galloylquinic acid derivative. A significant negative correlation (P < 0.05) was observed between the total phenolic content and the antioxidant activity. These results provide that Arbutus unedo leaves should be considered as a promising source of natural antioxidants, and as ingredients in complimentary food and nutraceutical products.

scholarly journals Antioxidant properties of species from the Brazilian cerrado by different assays

2013 ◽  
Vol 15 (4) ◽  
pp. 520-528 ◽  
Author(s):  
K.S. Farias ◽  
T.S.N. Santos ◽  
M.R.A.B. Paiva ◽  
S.M.L. Almeida ◽  
P.T. Guedes ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Reducing Power ◽  
Total Phenolic ◽  
Brazilian Cerrado ◽  
Medicinal Plant Extracts ◽  
Ethanolic Extracts ◽  
Total Antioxidant ◽  
Promising Source

The purpose of this study was to screen the antioxidant activity of medicinal plant extracts from the Brazilian cerrado, through other methods than the total phenolic content and its correlation with the antioxidant activity. Ethanolic extracts of ten species were evaluated through three antioxidant assays, in vitro, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant activity and reducing power; and by using the Folin-Ciocalteu method the total phenolic content was determined. Ethanolic extracts of Stryphnodendron obovatum, Cecropia pachystachya and Duguetia furfuraceae showed strong antioxidant activity (IC50<5 µg mL-1) in the DPPH free radical scavenging assay; the species Vernonia phosphorea, Hymenaea stignocarpa and Jacaranda ulei may also be highlighted. These results were confirmed in the assays of total antioxidant capacity and reducing power. The extracts of S. obovatum and V. phosphorea showed an abundant phenolic content; therefore, the phenolic content may play a role in the antioxidant activity. These two species, traditionally used in Brazil, showed great power in these assay systems and may be a promising source for the development of natural antioxidants and future candidates for phytochemical and pharmacological studies in related diseases.

Antiradical and Cytotoxic Activity of Different Helichrysum Plicatum Flower Extracts

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Dubravka Bigović ◽  
Katarina Šavikin ◽  
Teodora Janković ◽  
Nebojša Menković ◽  
Gordana Zdunić ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Total Phenolic Content ◽  
Antiradical Activity ◽  
Phenolic Content ◽  
Human Cancer ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Moderate Activity

Flowers of Helichrysum plicatum were extracted under different experimental conditions, and their antioxidant activity was determined by DPPH radical scavenging assay. Extracts obtained with higher concentration of ethyl acetate (90% or 100%) were found to contain the greatest amount of total phenolics (> 250 mg gallic acid equivalents/g of dried extract), and high correlation between total phenolic content and antiradical activity was observed (r = −0.79). Based on the total phenolic content and antiradical activity, some extracts were selected for investigation of cytotoxic activity toward PC3, HeLa and K562 human cancer cell lines in vitro. All tested extracts exhibited moderate activity against HeLa cells (41.9-42.1 μg/mL), whereas the extract obtained with 100% ethyl acetate was the most active against K562 and PC3 cell lines (25.9 and 39.2 μg/mL, respectively). Statistical analysis revealed significant correlation between total phenolic content and cytotoxic activity against PC3 and K562 cells. HPLC identification of phenolic compounds from the extracts indicated the presence of apigenin, naringenin and kaempferol as free aglycones, and glycosides of apigenin, naringenin, quercetin and kaempferol. Among aglycones, kaempferol displayed moderate cytostatic activity against all cell lines (24.8-64.7 μM).

scholarly journals Phenolic compounds of herbal infusions obtained from some species of the Lamiaceae family

2018 ◽  
Vol 31 (4) ◽  
pp. 194-199 ◽  
Author(s):  
Mariia Shanaida ◽  
Olena Golembiovska ◽  
Nataliia Hudz ◽  
Piotr P. Wieczorek
Keyword(s):  
Gallic Acid ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Hplc Method ◽  
Hydroxycinnamic Acids ◽  
Total Phenolic ◽  
Predominant Component ◽  
The Individual ◽  
Herbal Infusions

Abstract The present investigation was to estimate the total phenolic content and composition of flavonoids and hydroxycinnamic acids in herbal infusions obtained from aerial parts of three Lamiaceae species (Dracocephalum moldavica, Ocimum americanum and Satureja hortensis). The total phenolic content of herbal infusions was determined using a spectrophotometric method, whereas the individual phenolics were assessed by high-performance liquid chromatography (HPLC). The HPLC method was developed and validated. The total phenolic content was measured by applying the Folin-Ciocalteu method with reference to gallic acid. Results were in the range from 29.39 to 65.38 mg estimated as gallic acid equivalents per gram of dry herb. The phenolic profile was, in turn, analysed by HPLC and consisted of gallic acid, hydroxycinnamic acids (caffeic, chlorogenic, ferulic, and rosmarinic) and flavonoids (rutin, hyperoside, quercitrin, quercetin, apigenin, apigenin-7-glucoside and catechin) in different concentrations. Rosmarinic acid was the predominant component among the hydroxycinnamic acids in herbal infusions of all three plants. This was found to be in the range of 3.64 to 5.28 mg per gram of dry herb. Apigenin-7-glucoside, quercitrin and hyperoside were the prevailing flavonoid components of the infusions.

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