scholarly journals Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Akankwasa Gilbert ◽  
An Changjuan ◽  
Cheng Guixue ◽  
Liu Jianhua ◽  
Qin Xiaosong
Keyword(s):  
Matrix Metalloproteinase ◽  
Membranous Nephropathy ◽  
Healthy Subjects ◽  
Kidney Injury ◽  
Matrix Metalloproteinase 9 ◽  
Idiopathic Membranous Nephropathy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Glomerular Diseases ◽  
Metalloproteinase 9

Aim. Idiopathic membranous nephropathy (IMN) has a varied clinical course that requires accurate prediction as a prerequisite for treatment administration. Currently, its prognosis relies on proteinuria, a clinical parameter whose onset lags behind kidney injury. Increased urinary excretion of matrix metalloproteinase-9 (MMP-9) and nephrin has been reported in a number of IMN-like glomerular diseases in which they reflected disease severity. However, little or nothing is known of the importance of these biomarkers in IMN, a major cause of adult nephrotic syndrome. To highlight their potential, we measured both biomarkers and assessed their relationships with key parameters of renal function in IMN. Methods. We quantified urinary MMP-9 and nephrin in 107 biopsy-proven IMN patients and 70 healthy subjects by enzyme-linked immunosorbent assay (ELISA). We then compared biomarker levels between patients and healthy subjects and among patients with different clinical features. We also determined the relationship of each biomarker with proteinuria and the estimated glomerular filtration rate (eGFR). Results. Urinary MMP-9 and nephrin were significantly higher in IMN compared to healthy controls. Unlike nephrin, MMP-9 correlated significantly with proteinuria and was significantly higher among patients with nephrotic range proteinuria. Both biomarkers were correlated with eGFR, but only MMP-9 was significantly higher in patients with eGFR less than 90 ml/min/1.73 m2. Conclusion. Our findings suggest that urinary MMP-9 holds a greater potential than urinary nephrin in monitoring the severity of IMN.

scholarly journals Increased matrix metalloproteinase-9 in male elderly with low 25-hydroxy-vitamin D

2016 ◽  
Vol 35 (3) ◽  
pp. 171
Author(s):  
Pusparini Pusparini ◽  
Lie Tanu Merijanti ◽  
Novia I. Sudharma
Keyword(s):  
Vitamin D ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chi Square ◽  
Cross Sectional ◽  
25 Hydroxy Vitamin D ◽  
Male Subjects ◽  
Metalloproteinase 9 ◽  
Female Subjects

<p>Background <br />One of the extra-skeletal roles of vitamin D for health is associated with cardiovascular disease. Poor vitamin D status has been associated with vascular endothelial dysfunction. There were controversial results about the association between vitamin D deficiency and matrix metalloproteinase 9 (MMP-9) concentration. The purpose of the present study was to determine the concentrations of 25(OH) vitamin D [25(OH)D] in an elderly population and to find any association between 25(OH)D and MMP-9 concentrations.</p><p>Methods<br />This study was of cross-sectional design involving 160 male and female subjects aged 55–65 years, in South Jakarta, Indonesia. Determination of MMP-9 and 25(OH)D concentrations was done concurrently on subjects who met the inclusion and exclusion criteria after all study subjects had been selected. 25(OH)D and MMP-9 concentrations were assessed by direct competitive chemiluminescence immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) respectively. Statistical analysis used chi square and t tests.</p><p>Results<br />Mean 25(OH)D concentration in the study subjects was 14.4 ± 6.4 ng/mL. A total of 68.8% of subjects had a 25(OH)D level of &lt;20 ng/mL, and 31.2 % had a 25(OH)D level of &gt;20 ng/mL.There was an increased MMP-9 concentration in male subjects with a 25(OH)D level of &lt;20 ng/mL compared with subjects with 25(OH)D level of &gt;20 ng/mL (p=0.011), but not among female subjects (p=0.809).</p><p>Conclusion<br />The MMP-9 concentration was increased among male subjects with low level of (OH)D. This study confirmed that 25(OH)D concentration may have a potential role in endothelial function.</p>

scholarly journals Impact of Matrix Metalloproteinase-9 during Periodontitis and Cardiovascular Diseases

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1777
Author(s):  
Gaetano Isola ◽  
Alessandro Polizzi ◽  
Vincenzo Ronsivalle ◽  
Angela Alibrandi ◽  
Giuseppe Palazzo ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Healthy Subjects ◽  
Multivariate Regression Analysis ◽  
Matrix Metalloproteinase 9 ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Hs Crp ◽  
The Impact ◽  
Negative Predictor ◽  
Metalloproteinase 9

Matrix metalloproteinase-9 (MMP-9) has been shown to play a key role in endothelial function and perhaps pivotal in the correlation between periodontal disease and cardiovascular disease (CVD). For the study, the impact of MMP-9 of periodontitis and CVD on serum and saliva concentrations was analyzed. For the study patients with periodontitis (n = 31), CVD (n = 31), periodontitis + CVD (n = 31), and healthy patients (n = 31) were enrolled. Clinical and demographic characteristics as well as serum and salivary MMP-9 were evaluated. MMP-9 concentrations in serum and saliva were statistically elevated in patients with CVD (p < 0.01) and in patients with periodontitis plus CVD (p < 0.001) compared to patients with periodontitis and healthy subjects. Multivariate regression analysis showed that c-reactive protein (hs-CRP) was the only significant predictor for MMP-9 serum (p < 0.001), whereas hs-CRP (p < 0.001) and total cholesterol (p = 0.029) were the statistically significant salivary MMP-9 predictors. This study evidenced that patients with CVD and periodontitis + CVD presented elevated MMP-9 concentrations in serum and saliva compared to patients with periodontitis and healthy subjects. Furthermore, hs-CRP was a negative predictor of serum and salivary MMP-9.

Matrix Metalloproteinase-9 Levels are Associated with Brain Lesion and Persistent Venous Occlusion in Patients with Cerebral Venous Thrombosis

2021 ◽  
Author(s):  
Diana Aguiar de Sousa ◽  
Maria Conceição Pereira-Santos ◽  
Ana Serra-Caetano ◽  
Lia Lucas Neto ◽  
Ana Luísa Sousa ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Venous Thrombosis ◽  
Brain Damage ◽  
Cerebral Venous Thrombosis ◽  
Brain Lesion ◽  
Venous Occlusion ◽  
Matrix Metalloproteinase 9 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Venous Recanalization ◽  
Metalloproteinase 9

Abstract Background Elucidating mechanisms of brain damage in cerebral venous thrombosis (CVT) would be instrumental to develop targeted therapies and improve prognosis prediction. Matrix metalloproteinase-9 (MMP-9), a gelatinase that degrades major components of the basal lamina, has been associated to blood–brain barrier disruption. We aimed to assess, in patients with CVT, the temporal change in serum concentrations of MMP-9 and its association with key imaging and clinical outcomes. Methods Pathophysiology of Venous Infarction—PRediction of InfarctiOn and RecanalIzaTion in CVT (PRIORITy-CVT) was a multicenter prospective cohort study of patients with newly diagnosed CVT. Serial collection of peripheral blood samples performed on day 1, 3, and 8, and standardized magnetic resonance imaging on day 1, 8, and 90. MMP-9 was quantified using enzyme-linked immunosorbent assay in 59 patients and 22 healthy controls. Primary outcomes were parenchymal brain lesion, early evolution of brain lesion, early recanalization, and functional outcome on day 90. Results CVT patients with parenchymal brain lesion had higher baseline concentrations of MMP-9 compared with controls (adjusted p = 0.001). The area under receiver operating characteristic curve value for MMP-9 for predicting brain lesion was 0.71 (95% confidence interval [CI]: 0.57–0.85, p = 0.009). Patients with venous recanalization showed early decline of circulating MMP-9 and significantly lower levels on day 8 (p = 0.021). Higher MMP-9 on day 8 was associated with persistent venous occlusion (odds ratio: 1.20 [per 20 ng/mL], 95% CI: 1.02–1.43, p = 0.030). Conclusion We report a novel relationship among MMP-9, parenchymal brain damage, and early venous recanalization, suggesting that circulating MMP-9 is a dynamic marker of brain tissue damage in patients with CVT.

scholarly journals Binding of Pro-Matrix Metalloproteinase 9 by Fusobacterium nucleatum subsp. nucleatum as a Mechanism To Promote the Invasion of a Reconstituted Basement Membrane

2004 ◽  
Vol 72 (10) ◽  
pp. 6160-6163 ◽  
Author(s):  
Renée Gendron ◽  
Pascale Plamondon ◽  
Daniel Grenier
Keyword(s):  
Basement Membrane ◽  
Matrix Metalloproteinase ◽  
Colorimetric Assay ◽  
Active Form ◽  
Fusobacterium Nucleatum ◽  
Matrix Metalloproteinase 9 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potential Contribution ◽  
Invasive Potential ◽  
Metalloproteinase 9

ABSTRACT In this study, we investigated the ability of Fusobacterium nucleatum subsp. nucleatum to increase its tissue-invasive potential by acquiring cell-associated human matrix metalloproteinase 9 (MMP-9) activity. Binding of pro-MMP-9 to fusobacteria was demonstrated by enzyme-linked immunosorbent assay. Zymography and a colorimetric assay showed that bound pro-MMP-9 can be converted into a proteolytically active form. The potential contribution of this acquired host activity in tissue invasion was demonstrated using a reconstituted basement membrane (Matrigel).

scholarly journals Expression of Matrix Metalloproteinase-9 in Different Histopathological Variants of Ameloblastoma: A Cross-sectional Study

2021 ◽  
Author(s):  
Revati Shailesh Deshmukh ◽  
Priya Nimish Deo ◽  
Surekha Laxman Chavan ◽  
Prasad Kango
Keyword(s):  
Matrix Metalloproteinase ◽  
Tumour Progression ◽  
Matrix Metalloproteinase 9 ◽  
High Rate ◽  
P Value ◽  
Biological Behaviour ◽  
Cross Sectional ◽  
Intense Staining ◽  
Variable Expression ◽  
Metalloproteinase 9

Introduction: Ameloblastomas are benign and the most common odontogenic neoplasms with many histopathological subtypes depending on the predominant pattern. They are known for their aggressive behaviour. As ameloblastomas have a high rate of recurrence, it is necessary to understand the biological behaviour of these neoplasms. Matrix Metalloproteinase-9 (MMP-9) is an enzyme that belongs to Metalloproteinases family and is known to degrade the Extracellular Matrix (ECM) and facilitate tumour progression. Evaluation of the expression of MMP-9 in ameloblastomas could contribute in understanding its biological behaviour. Aim: To analyse the expression of MMP-9 in different histopathological variants of ameloblastoma. Materials and Methods: A cross-sectional observational study was done in the Department of Oral Pathology and Microbiology, Bharati Vidyapeeth Deemed to be University, Dental College and Hospital, Pune, India. A total of 30 cases were selected for this study. The study was carried out for a duration of two years from April 2018 to April 2020. The MMP-9 expression was studied by immunohistochemical staining. The statistical comparison was done using Chi-square test between groups, p-value <0.05 was considered significant. The data was statistically analysed using Statistical Package for Social Sciences (SPSS) version 22.0, IBM Corporation, USA for MS Windows. Results: A total of 30 paraffin embedded archival tissue blocks were selected for this study. Among them nine cases were of Plexiform Ameloblastoma, eight of Unicystic Ameloblastoma, three of Acanthomatous Ameloblastoma, four of Desmoplastic Ameloblastoma and six were of Follicular Ameloblastomas. The MMP-9 showed variable expression in different histopathological subtypes of ameloblastoma. This difference was statistically significant between Plexiform and Acanthomatous as well as Plexiform and Follicular variants (p<0.05). A 66.7% (6 out of 9 samples) of Plexiform Ameloblastoma showed intense staining for MMP-9. Conclusion: Expression of MMP-9 varies in different histopathological variants of ameloblastoma and may not have an association with biological behaviour and aggressiveness.

scholarly journals Cardiac aging is initiated by matrix metalloproteinase-9-mediated endothelial dysfunction

2014 ◽  
Vol 306 (10) ◽  
pp. H1398-H1407 ◽  
Author(s):  
Andriy Yabluchanskiy ◽  
Yonggang Ma ◽  
Ying Ann Chiao ◽  
Elizabeth F. Lopez ◽  
Andrew P. Voorhees ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Vascular Permeability ◽  
Oxygen Demand ◽  
Matrix Metalloproteinase 9 ◽  
Lv Function ◽  
Proteomic Profiling ◽  
Cross Sectional ◽  
Cardiac Aging ◽  
Myocyte Hypertrophy ◽  
Metalloproteinase 9

Aging is linked to increased matrix metalloproteinase-9 (MMP-9) expression and extracellular matrix turnover, as well as a decline in function of the left ventricle (LV). Previously, we demonstrated that C57BL/6J wild-type (WT) mice > 18 mo of age show impaired diastolic function, which was attenuated by MMP-9 deletion. To evaluate mechanisms that initiate the development of cardiac dysfunction, we compared the LVs of 6–9- and 15–18-mo-old WT and MMP-9 null (Null) mice. All groups showed similar LV function by echocardiography, indicating that dysfunction had not yet developed in the older group. Myocyte nuclei numbers and cross-sectional areas increased in both WT and Null 15–18-mo mice compared with young controls, indicating myocyte hypertrophy. Myocyte hypertrophy leads to an increased oxygen demand, and both WT and Null 15–18-mo mice showed an increase in angiogenic signaling. Plasma proteomic profiling and LV analysis revealed a threefold increase in von Willebrand factor and fivefold increase in vascular endothelial growth factor in WT 15–18-mo mice, which were further elevated in Null mice. In contrast to the upregulation of angiogenic stimulating factors, actual LV vessel numbers increased only in the 15–18-mo Null LV. The 15–18-mo WT showed amplified expression of inflammatory genes related to angiogenesis, including C-C chemokine receptor (CCR)7, CCR10, interleukin (IL)-1f8, IL-13, and IL-20 (all, P < 0.05), and these increases were blunted by MMP-9 deletion (all, P < 0.05). To measure vascular permeability as an index of endothelial function, we injected mice with FITC-labeled dextran. The 15–18-mo WT LV showed increased vascular permeability compared with young WT controls and 15–18-mo Null mice. Combined, our findings revealed that MMP-9 deletion improves angiogenesis, attenuates inflammation, and prevents vascular leakiness in the setting of cardiac aging.

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