scholarly journals Proteomic Analysis Reveals Growth Inhibition of Coriolus versicolor by Methanol Extracts of Cinnamomum camphora Xylem

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Quan Li ◽  
Xiangyang Li ◽  
Hui Lin
Keyword(s):  
Cellular Localization ◽  
Nitrogen Compound ◽  
Phosphoglycerate Kinase ◽  
Coriolus Versicolor ◽  
Metabolic Processes ◽  
Oxidoreductase Activity ◽  
Methanol Extracts ◽  
Functional Aspects ◽  
Differential Proteins ◽  
Resistant Tree

The extracts of decay-resistant tree species are important research objects for the future development of wood preservatives. To understand the antifungal mechanisms of Coriolus versicolor inhibition with methanol extracts of C. camphora xylem, the protein profiles of C. versicolor were analyzed using 2-DE followed by MALDI-TOF/MS and bioinformatic analyses. The results showed that 41 protein spots were obviously changed among the 366-385 protein spots of C. versicolor treated with methanol extracts of C. camphora xylem. Twenty-one protein spots were upregulated, and 20 protein spots were downregulated. Cellular localization was performed to identify these differential proteins, and biological process and functional analysis found that 9 of these proteins were in the cytoplasm, 6 were intracellular, and 5 were in the mitochondrion. A total of 18.8% were mapped to small-molecule metabolic processes, 12.5% to cellular amino acid metabolic processes, and 10.9% to cellular nitrogen compound metabolic processes. Twenty-five percent of the differential proteins were associated with ion bonding, 15% with oxidoreductase activity, and 15% with ATPase activity and transmembrane transport activity. Downregulated expression of aspartate aminotransferase, ATP synthase alpha chain, DEAD/DEAH-box helicase, and phosphoglycerate kinase showed that the methanol extracts of C. camphora xylem disrupted functional aspects such as nitrogen and carbon metabolism, energy metabolism, hormone signal response, and glucose metabolism, eventually leading to C. versicolor inhibition.

scholarly journals Glycosylation of matrix metalloproteases and tissue inhibitors: present state, challenges and opportunities

2016 ◽  
Vol 473 (11) ◽  
pp. 1471-1482 ◽  
Author(s):  
Lise Boon ◽  
Estefania Ugarte-Berzal ◽  
Jennifer Vandooren ◽  
Ghislain Opdenakker
Keyword(s):  
Matrix Metalloproteinases ◽  
Present State ◽  
Cellular Localization ◽  
Current Knowledge ◽  
Matrix Metalloproteases ◽  
Inhibitor Binding ◽  
Attachment Sites ◽  
Functional Aspects ◽  
Functional Implications ◽  
Challenges And Opportunities

Current knowledge about the glycosylation of matrix metalloproteinases (MMPs) and the inhibitors of metalloproteinases (TIMPs) is reviewed. Whereas structural and functional aspects of the glycobiology of many MMPs is unknown, research on MMP-9 and MMP-14 glycosylation reveals important functional implications, such as altered inhibitor binding and cellular localization. This, together with the fact that MMPs contain conserved and many potential attachment sites for N-linked and O-linked oligosaccharides, proves the need for further studies on MMP glycobiology.

Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolite Tuff as a Potential Novel Food Supplement

2021 ◽  
Vol 60 (1) ◽  
Author(s):  
Danka Matijašević ◽  
Milena Pantić ◽  
Nemanja Stanisavljević ◽  
Sanja Jevtić ◽  
Nevenka Rajić ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Enzymes ◽  
Antioxidant Properties ◽  
Coriolus Versicolor ◽  
World Population ◽  
Antioxidant Power ◽  
Methanol Extracts ◽  
Biological Potential ◽  
The Impact ◽  
Zeolite Tuff

Research background. In the recent years, considerable attention has been given to selenium (Se) status since its deficiency is linked with various disorders and affects at least 13 % of world population. Additionally, mushrooms are known to possess pronounced capacity for absorption of various micronutrients, including Se, from soil/substrate. Here, the possibility of using Se-rich zeolite tuff as a supplement for production of selenized mushroom is investigated. Further, the impact of enrichment on the activity of antioxidant enzymes and biological potential of Coriolus versicolor medicinal mushroom is studied. Experimental approach. Se(IV)- and Se(VI)-modified natural zeolitic tuff from the Serbian deposit Zlatokop was used as substrate supplement in mushroom cultivation. To examine effectiveness of selenium enrichment, beside inductively coupled plasma mass spectrometry (ICP-MS) analysis of total selenium content, determination of antioxidant enzymes in fresh fruiting bodies as well as testing of biological potential of methanol extracts was done. Antioxidant activity was evaluated using tests pertaining to different ways of antioxidant action: inhibition of lipid peroxidation, DPPH free radical scavenging assay, ferric-reducing antioxidant power assay and chelating ability on ferrous ions. The antibacterial activity against foodborne pathogens was measured by broth microdilution assay. Additionally, chemical composition of prepared extracts was studied using UV-Vis and FTIR spectroscopy analyses. Results and conclusions. Content of selenium detected in biofortified C. versicolor was even 470 times higher compared to control ((140.7±3.8) vs (0.3±0.1) µg/g dry mass), proving that Se-rich zeolite tuff is excellent supplement for mushroom production. Further, the results of monitoring the activity of antioxidant enzymes revealed that most of the Se-enriched mushrooms exhibited higher superoxide dismutase (SOD) and catalase (CAT) and lower glutathione peroxidase (GSH-Px) activity than control. Due to elevated level of enzymes, selenated mushrooms could quickly respond to superoxide radicals, formed as a result of detachment, and thus presumably preserve quality for a longer period of time. Investigation of biological potential indicated that Se-enriched mushroom methanol extracts, generally, expressed enhanced antioxidant properties. Additionally, extracts asserted antibacterial activity against all tested pathogenic microorganisms. Novelty and scientific contribution. Cultivation of mushrooms on Se-enriched zeolite tuff is a new technological approach for obtaining Se-fortified food/supplements with enhanced antioxidant and antibacterial activity.

scholarly journals Cellular Localization of Uridine 5′-Diphosphate-N-Acetyl-d-Glucosamine Oxidoreductase Activity in Citrobacter freundii

1976 ◽  
Vol 127 (2) ◽  
pp. 1032-1035
Author(s):  
Robert W. Wheat ◽  
Robert O. Barrow ◽  
Geoffrey A. Land
Keyword(s):  
Cellular Localization ◽  
Citrobacter Freundii ◽  
Oxidoreductase Activity

scholarly journals Comparing Transcriptome Profiles of Saccharomyces Cerevisiae Cells Exposed to Cadmium Selenide/Zinc Sulfide and Indium Phosphide/Zinc Sulfide

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 428
Author(s):  
Cullen Horstmann ◽  
Kyoungtae Kim
Keyword(s):  
Gene Expression ◽  
Zinc Sulfide ◽  
Metal Ion ◽  
Expression Profiles ◽  
Ion Homeostasis ◽  
Nitrogen Compound ◽  
Transmembrane Transport ◽  
Metabolic Processes ◽  
Mechanisms Of Toxicity ◽  
Metal Ion Homeostasis

The primary focus of our research was to obtain global gene expression data in baker’s yeast exposed to sub-lethal doses of quantum dots (QDs), such as green-emitting CdSe/ZnS and InP/ZnS, to reveal novel insights on their unique mechanisms of toxicity. Despite their promising applications, their toxicity and long-lasting effects on the environment are not well understood. To assess toxicity, we conducted cell viability assays, ROS detection assays, and assessed their effects on the trafficking of Vps10-GFP toward the trans-Golgi network with confocal microscopy. Most notably, we used RNA-sequencing (RNA-seq) to obtain gene expression profiles and gene identities of differentially expressed genes (DEGs) in QD-treated yeast. We found CdSe/ZnS QDs significantly altered genes implicated in carboxylic acid, amino acid, nitrogen compounds, protein metabolic processes, transmembrane transport, cellular homeostasis, cell wall organization, translation, and ribosomal biogenesis. Additionally, we found InP/ZnS QDs to alter genes associated with oxidation-reduction, transmembrane transport, metal ion homeostasis, cellular component organization, translation, and protein and nitrogen compound metabolic processes. Interestingly, we observed an increase in reactive oxygen species (ROS) in CdSe/ZnS-treated cells and a decrease in ROS levels in InP/ZnS-treated cells. Nevertheless, we concluded that both QDs modestly contributed cytotoxic effects on the budding yeast.

N-Methyl-d-Aspartate Receptor Subunit NR3A in the Retina: Developmental Expression, Cellular Localization, and Functional Aspects

2003 ◽  
Vol 44 (10) ◽  
pp. 4451 ◽  
Author(s):  
Nikolaus J. Sucher ◽  
Konrad Kohler ◽  
Lalitha Tenneti ◽  
Hon-kit Wong ◽  
Tatiana Gru¨nder ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Developmental Expression ◽  
Receptor Subunit ◽  
Aspartate Receptor ◽  
Functional Aspects

scholarly journals Liver Metabolome and Proteome Response of Turbot (Scophthalmus maximus) to Lysine and Leucine in Free and Dipeptide Forms

2021 ◽  
Vol 8 ◽  
Author(s):  
Yuliang Wei ◽  
Benxiang Li ◽  
Houguo Xu ◽  
Mengqing Liang
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Scophthalmus Maximus ◽  
Protein Digestion ◽  
Metabolic Processes ◽  
Differential Proteins ◽  
Protein Synthesis And Degradation ◽  
Synthesis And Degradation

Omics approaches provide more metabolic information to explain the relationship between dietary nutrition and fish growth. This study aimed to explore the metabolome and proteome response of turbot (Scophthalmus maximus) fed diets containing lysine and leucine in free and dipeptide forms by the approaches of integrated liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomics and isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics. Plant protein-based diets were formulated to contain the equivalent of lysine and leucine in free amino acid [crystalline amino acid (CAA)] and synthetic Lys-Leu (Lys-Leu) forms. The metabolome and proteome profiles of the liver were screened in fish fed either the CAA diet or the Lys-Leu diet after an 8-week feeding trial. Fish fed the Lys-Leu diet showed a significantly higher final body weight and a specific growth rate compared with fish fed the CAA diet. Protein- and amino acid-related metabolic processes in the liver were identified between the Lys-Leu and CAA groups based on differential metabolites and proteins. The proteolytic enzymes and amino acid transporters from differential proteins of the liver showed that the process of protein digestion and absorption may be affected by the different forms of lysine and leucine in the feed. A mechanistic target of rapamycin complex 1 and ubiquitin proteasome pathways were identified by differential proteins, which were involved in the processes of protein synthesis and degradation in the liver. Lysine degradation, tryptophan metabolism, alanine, aspartate, and glutamate metabolism, arginine biosynthesis, arginine and proline metabolism, and glycine, serine, and threonine metabolism were identified based on differential metabolites and proteins, which showed that the metabolism of various amino acids, including lysine, had been affected by both the CAA and Lys-Leu groups. In conclusion, the data of integrated metabonomics and proteomics suggested that different forms of lysine and leucine in the feed may affect liver metabolic processes including protein digestion and absorption, protein synthesis and degradation, and amino acid metabolism. In addition, a good correlation between differential metabolites and proteins was observed in amino acid metabolism by using the approaches of integrated LC-MS/MS-based metabolomics and iTRAQ-based proteomics.

The Immuno-Electron Microscopic Localization of the Mo-Fe Protein Component of Nitrogenase in Cells of Azotobacter Vinelandii

1973 ◽  
Vol 31 ◽  
pp. 574-575
Author(s):  
J. T. Stasny ◽  
R. C. Burns ◽  
R. W. F. Hardy
Keyword(s):  
Cellular Localization ◽  
Direct Evidence ◽  
Azotobacter Vinelandii ◽  
Intracellular Localization ◽  
Protein Component ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Fe Protein ◽  
Intracytoplasmic Membranes

Structure-functlon studies of biological N2-fixation have correlated the presence of the enzyme nitrogenase with increased numbers of intracytoplasmic membranes in Azotobacter. However no direct evidence has been provided for the internal cellular localization of any nitrogenase. Recent advances concerned with the crystallizatiorTand the electron microscopic characterization of the Mo-Fe protein component of Azotobacter nitrogenase, prompted the use of this purified protein to obtain antibodies (Ab) to be conjugated to electron dense markers for the intracellular localization of the protein by electron microscopy. The present study describes the use of ferritin conjugated to goat antitMo-Fe protein immunoglobulin (IgG) and the observations following its topical application to thin sections of N2-grown Azotobacter.

Mucin gene expression in OME: cellular localization

1998 ◽  
Vol 23 (3) ◽  
pp. 281-282
Author(s):  
Hutton ◽  
Guo ◽  
Birchall ◽  
Pearson
Keyword(s):  
Gene Expression ◽  
Cellular Localization ◽  
Mucin Gene

Functional Aspects of Language Acquisition: Another View

1978 ◽  
Vol 23 (9) ◽  
pp. 623-624
Author(s):  
MARIS MONITZ RODGON
Keyword(s):  
Language Acquisition ◽  
Functional Aspects
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