scholarly journals Comparison of the Effects of Repeated Applications of Platelet-Rich Plasma versus Platelet-Poor Plasma on Fat Graft Survival in Nude Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Junjie Li ◽  
Wei Chen ◽  
Xiao Shi ◽  
Pijun Yu
Keyword(s):  
Graft Survival ◽  
Platelet Rich Plasma ◽  
Capillary Density ◽  
Saline Group ◽  
Fat Grafting ◽  
Fat Graft ◽  
Control Group ◽  
Survival Ratio ◽  
Platelet Poor Plasma ◽  
Fat Grafts

Autologous fat grafting has been widely used for soft tissue filling in plastic surgery. Platelet-rich plasma (PRP) could play a wide role in health and disease because of containing a variety of growth factors and cytokines. Although previous studies have described the positive effect of autologous PRP mixed with fat grafts, only minimal improvements in fat graft survival have been reported. The present study is aimed at comparing the effects of PRP and platelet-poor plasma (PPP) on the survival and quality of fat grafting. We built a 180-day nude mouse model implanted with a fat graft supplemented with PRP, PPP, or saline, respectively. The above reagents (PRP, PPP, or saline) were injected two additional times after the initial engraftment. The survival ratio of the fat grafts and the capillary density in the PRP group were significantly higher than those in the PPP group and the saline group (control group) at 15, 30, 90, and 180 days posttransplantation ( P < 0.05 ). The survival ratio of the PPP group was higher than that of the saline group ( P < 0.05 ), but the capillary density in the PPP group was not significantly different from that in the saline group at any time point ( P > 0.05 ). We hence conclude that the repeated application of PRP or PPP three times can enhance the survival of fat grafts within 180 days. Moreover, the effect of PRP is superior to that of PPP.

The Effects of Vasonatrin Peptide on Fat Graft Viability: An Experimental Study

2021 ◽  
Author(s):  
Fatih Irmak ◽  
Mert Sizmaz ◽  
Selami Serhat Sirvan ◽  
Semra Karsidag ◽  
Aysim Ozagari
Keyword(s):  
Graft Survival ◽  
Cyst Formation ◽  
Capillary Density ◽  
Fat Graft ◽  
Control Group ◽  
Sprague Dawley ◽  
Fat Injection ◽  
Sterile Saline ◽  
Average Percentage ◽  
Fat Grafts

AbstractVasonatrin peptide (VNP) is a synthetic peptide that possesses vasodilatory, natriuretic, and anti-inflammatory properties. The authors aimed to analyze the effects of VNP on fat graft survival. Twenty Sprague–Dawley rats are randomly divided into two groups of 10. Fat grafts are harvested from the right inguinal region. After preparation, fat grafts are placed to the interscapular region. The first group of rats were administered VNP after their fat injection, while the second group received tail-vein injections of an equal volume of sterile saline following their fat injection. Experiment and control groups are evaluated according to their level of degeneration of adipocytes, fat necrosis, vacuolization, cyst formation in adipocytes, fibrosis of the fat tissue, capillary density, and CD31 immunohistochemical staining. Degeneration, vacuolization, and cyst formation in adipocytes were lower in the experiment group. Increased capillary density in the experiment group was demonstrated by CD31 antibody staining and by counting capillary density under a microscope. The average percentage of change in weight of the fat grafts in the experiment group was lower than that in the control group. The results indicate that VNP has some beneficial effects on fat graft survival by multiple independent mechanisms that influence both local and systemic homeostasis.

The Effect of Minocycline on Fat Graft Survival and Apoptotic Pathway

2019 ◽  
Vol 35 (01) ◽  
pp. 096-102 ◽  
Author(s):  
Kırdar Güney ◽  
Bora Özel ◽  
Cemile Seymen ◽  
Çiğdem Elmas ◽  
Serhan Tuncer ◽  
...  
Keyword(s):  
Graft Survival ◽  
Study Groups ◽  
Fat Grafting ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Fat Graft ◽  
Albino Rats ◽  
Apoptotic Pathway ◽  
Fat Grafts ◽  
Dorsal Area ◽  
Wistar Albino Rats

AbstractVariable absorption rate is one of the biggest problems of fat grafting and one of the most important causes of fat graft volume loss is apoptosis. Minocycline is a tetracycline derivative and besides its antibacterial capacity, it has been widely using for anti-apoptotic effects. This study was designed to investigate the effect of minocycline on fat graft survival and adipocyte apoptosis. A total of two main and eight subgroups were designed and a total of 48 experimental animals, 6 in each group, were used. Fat grafts are obtained from Wistar albino rats and implanted to dorsal area of rats. Local and systemic minocycline was applied in the study groups. On the 9th day, apoptotic cells were detected by the terminal deoxynucleotidyl transferase dUTP nick end labeling method and on the 90th day morphologic characteristics and viability of adipocytes were evaluated using histologic and immunohistochemical methods and statistically compared. This study revealed that the fat grafts were bigger, and they kept their structures better and they were more vascular in the minocycline groups and apoptosis was significantly lower in the minocycline groups. The authors demonstrated that minocycline increases fat graft survival and statistical improvement in apoptosis inhibition via using minocycline therapy has been shown.

Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c

2021 ◽  
Vol 53 (4) ◽  
pp. 501-510
Author(s):  
Xiaoyan Hao ◽  
Yuan Guo ◽  
Rui Wang ◽  
Xueyuan Yu ◽  
Lin He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graft Survival ◽  
Macrophage Polarization ◽  
Fat Grafting ◽  
Fat Graft ◽  
Fat Grafts ◽  
Enhancer Binding Protein ◽  
Key Factor ◽  
Anti Inflammatory

Abstract The rate of fat graft survival is a critical aspect of successful surgery and has been a matter of concern for over 20 years. Owing to their anti-inflammatory effects and regenerative property, adipose-derived mesenchymal stem cells (AD-MSCs) have been adapted for clinical application in fat grafting, although the mechanism underlying their action remains unclear. Recently, exosomes derived from MSCs were suggested as a better alternative, and these exosomes have also been applied in diverse clinical therapies. Accumulating evidence suggests that MSCs modulate macrophage differentiation via exosome secretion, and the connection between macrophage regulation and the rate of fat graft survival has been established. Here, we identified that let-7c, the key factor in the regulatory process, is shuttled by AD-MSC-derived exosomes to downregulate the transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ. The downregulation of C/EBP-δ resulted in the attenuation of pro-inflammatory M1 macrophages and elevation of anti-inflammatory M2 macrophages. These results suggest that AD-MSC-derived exosomes promote the survival of fat grafts by regulating macrophage polarization via let-7c. This is the first study to elucidate the mechanism underlying the promotion of the fat graft survival rate by AD-MSCs and to evaluate the immunotherapeutic potential of AD-MSC-derived exosomes in fat grafting.

A Novel Fat Making Strategy for Adipose-Derived Progenitor Cells Enrichment Fat Improves Fat Graft Survival

2021 ◽  
Author(s):  
Pengju Fan ◽  
Man Fang ◽  
Jingjing Li ◽  
Mario G Solari ◽  
Dingyu Wu ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retention Rate ◽  
Capillary Density ◽  
Fat Grafting ◽  
Fat Graft ◽  
Histological Structure ◽  
Fat Grafts ◽  
Potential Strategy ◽  
Novel Strategy ◽  
Underlying Mechanisms

Abstract Background A low survival rate is one of the main challenges in fat grafting. Objectives This study aimed to evaluate whether the microfat obtained using a novel strategy promoted the survival and retention of fat grafts. Methods A 5 mm diameter blunt tip cannula with larger side holes (~30 mm 2 per hole) was used to obtain macrofat. Subsequently, a novel strategy based on a newly invented extracorporeal cutting device was used to cut the macrofat into microfat, which was named adipose-derived progenitor cells enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both groups of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER characteristics and underlying mechanisms. Results The retention rate of fat grafts in AER fat-treated animals was significantly higher than that in the Coleman group (54.6±13% vs. 34.8±9%, P&lt;0.05) after 10 weeks. AER fat contained more DPP4 + progenitor cells (3.3±0.61×10 3 vs. 2.0±0.46×10 3 cells/mL, P&lt;0.05), adipose-derived plastic-adherent cells (6.0±1.10×10 4 vs. 2.6±0.17×10 4 cells/mL, P&lt;0.001), and viable adipocytes as compared to Coleman fat. Moreover, histological analysis showed that AER fat graft had better histological structure and higher capillary density. Conclusions AER fat transplantation is a potential strategy to improve the survival and long-term retention of fat grafts. AER fat contained more DPP4 + progenitor cells.

The Effect of Locally Delivered Apocynin on Fat Graft Survival in an Experimental Rat Animal Model

2022 ◽  
Author(s):  
doğan çakan ◽  
sinem çiloglu ◽  
ekrem ramazan keskin
Keyword(s):  
Graft Survival ◽  
Survival Rates ◽  
Viable Cell ◽  
Saline Group ◽  
Fat Grafting ◽  
Male Rats ◽  
Fat Graft ◽  
Biophysical Parameters ◽  
Physical Measurements ◽  
Significant Difference

Objectives: We aimed to investigate the efficacy of locally delivered apocynin on fat graft survival in an experimental autologous fat grafting (AFG) model created in rats. Methods: Twenty-one Wistar albino male rats were included in this study. The 0.647 g mean weight grafts were harvested from the inguinal region and transferred to the nape of every rat. The subjects were randomly separated into three groups. Saline, dimethyl sulfoxide (DMSO) and apocynin, a dose of 20 mg/kg, solutions were applied once a day for 2 weeks. After 3 months, the rats were sacrificed. The evaluation of physical measurements (weight and volume) and survival rates of the grafts for volume (SRV) and weight (SRW), the viable cell count (VC) with the MTT assay, and histopathological parameters were done. Results: All biophysical parameters were found to be significantly higher in the apocynin group compared to other groups (p < .05). In the MTT test, the saline group was normalized to 100%. According to this, DMSO and apocynin groups’ means were 106% and 163%, respectively. The VC was significantly higher in the apocynin group than the other groups (p < .05). The VC was significantly higher in the DMSO group than in the saline group (p < .05). No significant difference was found in other comparisons performed according to biophysical and histopathological parameters (p > .05). Conclusion: The locally delivered apocynin decreases fat graft volume loss in an experimental AFG model. Consequently, apocynin can be used as an effective substance to increase graft survival.

A Systematic Review on Extracellular Vesicles-Enriched Fat Grafting: A Shifting Paradigm

2020 ◽  
Author(s):  
Mohammad Ghiasloo ◽  
Laura De Wilde ◽  
Kashika Singh ◽  
Patrick Tonnard ◽  
Alexis Verpaele ◽  
...  
Keyword(s):  
Systematic Review ◽  
Graft Survival ◽  
Extracellular Vesicles ◽  
Retention Rate ◽  
Fat Grafting ◽  
Retention Rates ◽  
Fat Graft ◽  
Cochrane Central Register

Abstract Background Recent evidence confirms that mesenchymal stem cells (MSCs) facilitate angiogenesis mainly through paracrine function. Extracellular vesicles (EVs) are regarded as key components of the cell secretome, possessing functional properties of their source cells. Subsequently, MSC-EVs have emerged as a novel cell-free approach to improve fat graft retention rate. Objectives To provide a systematic review of all studies reporting the use of MSC-EVs to improve graft retention rate. Methods A systematic search was undertaken using the Embase, PubMed and the Cochrane Central Register of Controlled Trials databases. Outcome measures included donor/receptor organism of the fat graft, study model, intervention groups, evaluation intervals, EV research data, in vitro and in vivo results. Results Of the total 1717 articles, 62 full-texts were screened. Seven studies reporting on 294mice were included. Overall, EV treated groups showed higher graft retention rates compared to untreated groups. Notably, retention rate was similar following EV- and MSC-treatment. In addition to reduced inflammation, graft enrichment with EVs resulted in early revascularization and better graft integrity. Interestingly, hypoxic preconditioning of MSCs improved their beneficial paracrine effects and led to a more proangiogenic EV population, as observed by both in vitro and in vivo results. Conclusions MSC-EVs appear to offer an interesting cell-free alternative to improve fat graft survival. While their clinical relevance remains to be determined, it is clear that not the cells, but their secretome is essential for graft survival. Thus, a paradigm shift from cell-assisted lipotransfer towards ‘secretome-assisted lipotransfer’ is well on its way.

The Effect of Combined Use of Platelet Rich Plasma and Adipose Derived Stem Cells on Fat Graft Survival

2012 ◽  
Vol 130 ◽  
pp. 71
Author(s):  
Nevra Seyhan ◽  
Dogan Alhan ◽  
Ali Ugur Ural ◽  
Armagan Unal ◽  
Mustafa Cihad Avunduk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graft Survival ◽  
Platelet Rich Plasma ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Combined Use

scholarly journals Effects of increased proliferation of human adipose tissue-derived mesenchymal stem cells by sphingosylphosphorylcholine on the survival of cryopreserved fat grafts

2018 ◽  
Vol 70 (3) ◽  
pp. 543-549
Author(s):  
Yong Bae ◽  
Ji Song ◽  
Kyeong Nam ◽  
Joo Kim ◽  
Su Nam
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Nude Mice ◽  
Soft Tissue Augmentation ◽  
Fat Graft ◽  
Saline Control ◽  
Control Group ◽  
Fat Tissue ◽  
Lipid Molecule ◽  
Fat Grafts

The use of cryopreserved adipose tissue for soft-tissue augmentation is common, but the unpredictability of fat graft viability remains a limitation. Human adipose-derived stem cells (hADSC) have been introduced to enhance viability and improve the survival of transplanted fat tissue. Sphingosylphosphorylcholine (SPC) is a bioactive lipid molecule involved in various cellular responses. SPC stimulates the proliferation of various cell types such as hADSC. We demonstrated the effects of hADSC and SPC on the survival of cryopreserved fat grafts in nude mice. The cryopreserved fat grafts were treated with hADSC or hADSC+SPC, and a normal saline (control) mixture in BALB/c male nude mice. We examined the weight and volume of the mice in each group (n=11) at 8 weeks after transplantation to evaluate the survival of the fat tissue. The hADSC group showed increased weight and volume compared with the control group. The hADSC+SPC group showed a higher survival rate in terms of weight and volume than the control or hADSC group. In addition, the hADSC+SPC treatment significantly increased the expression of angiogenic factors. These results suggest the potential clinical utility of hADSC+SPC.

scholarly journals Effectiveness of Platelet Rich Plasma for the Management of Knee Osteoarthritis

2021 ◽  
Vol 15 (7) ◽  
pp. 1553-1556
Author(s):  
Adnan Qamar ◽  
Saima Naz Mohsin ◽  
Uzma Nasim Siddiqui ◽  
Sana Naz ◽  
Sana Danish
Keyword(s):  
Knee Osteoarthritis ◽  
Normal Saline ◽  
Platelet Rich Plasma ◽  
Controlled Trial ◽  
Saline Group ◽  
Control Group ◽  
Plasma Therapy ◽  
Vas Score ◽  
To Receive

Aim: To determine impact of platelet rich plasma therapy for the management of knee osteoarthritis. Study design: Prospective double-blinded, add –on randomized, placebo-controlled trial Place and duration of study: Department of Orthopedics, Shaikh Zayed Hospital Lahore from 1st January 2019 to 30th June 2019 Methodology: Fifty volunteer participants fulfilling inclusion criteria were enrolled. One hundred knees of patients were randomly allocated into two groups. Knees were assigned either of the two groups. Platelet rich plasma group which was assigned to receive 5ml of platelet rich plasma and normal saline (NS) group which was assigned to receive 5ml of NS labeled as control group. All patients given 3 successive intra-articular injections of 5 mL of autologous platelet rich plasma or 5ml of normal saline was given at weekly intervals. Patients were blinded and subjected to a standardized injection protocol and the intensity of pain was assessed on visual analog scale (VAS) for pain. Results: The VAS scores decreased from 50.9±14.7 at baseline assessment to 43.6±16.2 at 1-month follow-up after completion of therapy for PRP group (p<0.0203). The improvement was maintained from the end of the therapy to 3 and 6 months’ follow-up, as measure of VAS score as 30.54±11.8 and 20.2±8.6 respectively (p<0.0001). In normal saline group, there was very slight decrease in VAS score from baseline i.e. 49.8±19.5 to 48±22.7, 44±16.6, and 42±21.7 at 1, 3 and 6 months interval. Conclusion: Platelet rich plasma therapy can provide effective pain control up to 6 months post injection in knee osteoarthritis. Key words: Platelet rich plasma (PRP), Knee osteoarthritis (OA), Pain score

Sign in / Sign up

Export Citation Format

Share Document