scholarly journals Physicochemical Characterization and Polyphenolic Content of Beninese Honeys

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Sênan Christa Lokossou ◽  
Fidèle Paul Tchobo ◽  
Hounnankpon Yédomonhan ◽  
Mohamed Mansourou Soumanou
Keyword(s):  
Moisture Content ◽  
Phenolic Content ◽  
Physicochemical Characterization ◽  
Physicochemical Characteristics ◽  
Total Phenolic ◽  
Free Acidity ◽  
Flavonoid Content ◽  
Phenolic Contents ◽  
Polyphenolic Content ◽  
Phenolic And Flavonoid

The physicochemical and phytochemical analyses of honeys (n=50) from Sudanese, Sudano-Guinean, and Guinean areas of Benin were investigated. Results showed that dark amber is the dominant color. Moisture content ranged from 15.50% to 23.50%, and 72% of honeys respected the Codex Alimentarius recommendation. pH varied between 2.87 and 6.15, and free acidity ranged from 9.00 to 39.00 meq/kg. Electrical conductivity varied from 0.37 to 1.43 mS/cm. The content in fructose varied from 21.67% to 94.21%, and proline content ranged between 306.31 and 1187.93 mg/kg. All physicochemical characteristics varied with the areas. A negative correlation was found between pH and moisture content (r=-0.55; p<0.01). A positive correlation was established between pH and conductivity (r=0.79; p<0.01) and between proline and color (r=0.44; p<0.01). Total phenolic content varied between 55.97 and 224.99 mg GAE/100 g, and flavonoid content ranged between 1.43 and 29.81 mg CAE/100 g. Flavonoid was positively correlated with color (r=0.78; p<0.01) and proline (r=0.47; p<0.01). Tukey’s test revealed differences between total phenolic and flavonoid contents of honeys from five areas (p<0.01). In general, Sudanese and Sudano-Guinean honeys showed potential toward therapeutic applications because of their high phenolic contents.

scholarly journals Antioxidant potential of five commercially less valued leafy vegetables from Bangladesh

2018 ◽  
Vol 47 (4) ◽  
pp. 953-959
Author(s):  
Kishwar Jahan Shethi ◽  
Mohammad Zashim Uddin
Keyword(s):  
Phenolic Content ◽  
Leafy Vegetables ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phenolic And Flavonoid

To evaluate the antioxidant potential of less valued green leafy vegetables of Bangladesh, the methanolic extracts of Alternanthera sessilis, A. philoxeroides, A. paronychioides, Glinus oppositifolius and Portulaca oleracea were subjected to assay for total phenolic content, total flavonoid content and DPPH free radicle scavenging activity. Among the studied materials, A. sessilis showed the highest phenolic and flavonoid content. However, G. oppositifolius presented the lowest phenolic content (14.53 ± 0.48 mg of GAE/g of sample) and P. oleracea exhibited minimum (7.005 ± 0.25 mg of RUE/g of sample) flavonoid content. DPPH radical scavenging activity was found to increase with increase in the concentration of each individual material. A. philoxiroides (IC50 = 6.07 ± 0.27 μg/ml) was found as the most active in DPPH radical percentage inhibition, which was followed by A. sessilis (IC50 = 7.17 ± 0.67 μg/ml). Interestingly, A. paronychioides, in spite of having good amount of total phenolic and flavonoid content, showed less DPPH scavenging activity. No linear relationship was found to exist in case of the total phenolic, flavonoid content and DPPH scavenging activity among G. oppositifolius, P. oleracea and A. paronychioides. The results from different methods may differ substantially because each complex chemical reaction generates unique values. However, all the five plants exhibited creditable antioxidant activity and thus consumption of these vegetables may play important role in combating oxidative stresses and thereby help in retaining good health.

scholarly journals Study on Total Phenolic, Flavonoid and Antioxidant Capacity of Fish Singgang Extracts

2021 ◽  
pp. 12-21
Author(s):  
Anis Nafisah Jamain ◽  
Norhaslinda Ridzwan ◽  
Mimie Noratiqah Jumli ◽  
Norhayati Abd Hadi ◽  
Mohd Adzim Khalili Rohin ◽  
...  
Keyword(s):  
Moisture Content ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Control Sample ◽  
Ash Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Aim: To evaluate the ash and moisture contents, total phenolic content, total flavonoid content, and antioxidant potential of Terengganu singgang extracts. Study Design: Experimental study. Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between April 2019 and July 2019. Methodology: Samples comprised three types of singgang dishes, which were prepared, cooked, and then extracted with distilled water and ethanol (EtOH) in different strengths, 50%, 70%, and 100%. These singgang samples were chub mackerel (ST), Indian mackerel (SK), and a control sample with no fish(SC). Extracts were analyzed for their moisture and ash content. Also, the total phenolic content (TPC) was assayed using Folin-Ciocalteu reagent, while total flavonoid content (TFC) using AlCl3 colorimetric assay, and antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl (DPPH). The total antioxidant capacity (T-AOC) was also evaluated. Results: Experimental assays showed that the SC sample extracted in 100% EtOH produced the highest yield (3.7%). SK samples were lower than SC and ST in moisture content and ash content with 94.21%, 96.37% and 93.03% moisture content and 0.85%, 0.71%, and 0.96% ash content. Meanwhile, the extract of ST in 100% EtOH yielded the highest TPC (315.0 mg GAE/100g) and T-AC (8.8 U/mL) but the lowest in DPPH scavenging activity (12.2%). On the other hand, the extract of SK in 70% EtOH gave the highest TFC with 6485.3 mg QE/100g. The correlation of TFC and TPC with DPPH and T-AOC assays was positively significant. Conclusion: In conclusion, the ST extract yielded the best antioxidant capacity.

ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nur Azfa Shuib ◽  
Anwar Iqbal ◽  
Fatimatul Akmal Sulaiman ◽  
Izzatie Razak ◽  
Deny Susanti
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Disc Diffusion ◽  
Flavonoid Content ◽  
Disc Diffusion Method ◽  
Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

scholarly journals Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1244
Author(s):  
Ioanna K. Siakavella ◽  
Fotini Lamari ◽  
Dimitrios Papoulis ◽  
Malvina Orkoula ◽  
Patroula Gkolfi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Colloidal Stability ◽  
Skin Barrier ◽  
Physicochemical Characteristics ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Laser Scattering ◽  
The Impact ◽  
Phenolic And Flavonoid

Silver nanoparticles (AgNPs) were synthesized using hydroalcoholic extracts of dittany (Origanum dictamnus), sage (Salvia officinalis), sea buckthorn (Elaeagnus rhamnoides, syn. Hippophae rhamnoides), and calendula (Calendula officinalis) as reducing agents. AgNPs synthesized using NaBH4 and citric acid were used as control. The impact of the origin of the extract and preparation conditions (light, temperature, reaction time) on the properties of the synthesized AgNPs was investigated. The structure, morphology, composition, physicochemical characteristics, and colloidal stability were characterized using dynamic laser scattering (DLS), ultraviolet-visible spectrophotometry (UV–/Vis), XRD, X-ray fluorescence (XRF), TEM, and FTΙR. The reduction of total phenolic and flavonoid content of the extracts after the reaction of AgNPs synthesis was also determined. Low IC50 values for all types of AgNPs revealed good antioxidant activity, attributable to the phenolic and flavonoid content of their surface. The results suggest that plant extract selection is important to the green synthesis of AgNPs because it affects the kinetics of their synthesis as well as their morphology, physicochemical characteristics, and colloidal stability. In vitro permeation studies on porcine skin revealed that AgNPs remained at the upper layers of stratum corneum and did not penetrate the skin barrier after 4 h of cutaneous application suggesting the safety of their application on intact skin for a relatively short time.

scholarly journals Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia

2019 ◽  
Vol 20 (3) ◽  
pp. 656-663
Author(s):  
NURUL KHUMAIDA ◽  
MUHAMAD SYUKUR ◽  
MARIA BINTANG ◽  
WARAS NURCHOLIS
Keyword(s):  
Medicinal Plant ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Morphological Diversity ◽  
Dry Weight ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
West Java ◽  
Extract Yield ◽  
Phenolic And Flavonoid

Abstract. Khumaida N, Syukur M, Bintang M, Nurcholis W. 2019. Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia. Biodiversitas 20: 656-663. Curcuma aeruginosa is a rhizomatous medicinal plant with beneficial pharmacological activities. The aim of this work was to analyze the agro-morphological, extract yield, and phenolic content of ten C. aeruginosa accessions which were collected from different locations in Indonesia. Cultivation was carried out in the open field in West Java of Indonesia using a completely randomized design. Qualitative and quantitative parameters were used to investigate agro-morphological traits. Total phenolic and total flavonoids contents were determined in ethanol extracts of samples. The plants were phenotypically diverse, in which there were significant variations among the ten C. aeruginosa accessions in number of leaves, plant height, number of shoots, fresh weight of rhizome, and dry weight of rhizome characters. Variability in the total phenolic and total flavonoid contents ranged from 29.08-46.92 mg GAE/g, and 21.31-33.81 mg QE/g, respectively. Six accessions had high phenolic content and extract yield. Therefore, these accessions could be utilized for commercial scale and also showed a high potency for medicinal plant breeding programs.

scholarly journals TOTAL PHENOLIC AND FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY OF VITEX MONTEVIDENSIS (SPRENG) MOLDENKE - VERBENACEAE

2016 ◽  
Vol 38 (3) ◽  
pp. 1197
Author(s):  
Sideney Becker Onofre ◽  
Dirceu Abatti ◽  
Amarildo Antonio Tessaro ◽  
Alessandra Buss Tessaro
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Degenerative Diseases ◽  
Flavonoid Content ◽  
The Mean ◽  
Phenolic And Flavonoid

In recent years, a substantial amount of evidence has pointed to the key role of free radicals and other oxidants as the main culprits for aging and degenerative diseases associated with aging, such as cancer, cardiovascular diseases, cataract, decline of the immune system and brain dysfunctions. The objective of this work was therefore to detect variations in total phenol and flavonoid content, and in antioxidant activity of samples of V. montevidensis. Dried samples were submitted to extraction to obtain the hexane, ethyl acetate and ethanol fractions. Phytochemical prospecting and an analysis of the phenolic content and antioxidant activity was carried out. The data was analyzed according to the mean±standard deviation and submitted to analysis of variance followed by Tukey's test with a significance of (p < 0.05). Flavonoids, tannins, coumarins, terpenoids, steroids, alkaloids and anthraquinones were detected in the samples. The content of total flavonoids varied between 3.18±0,58 and 7.22±0,17 g/100g, while the total phenols ranged from 1.13±0.16 to 18.44±0.21 g/100g. The extracts produced EC50 between 339.75±6.56 and 278.33±23.11 μg/mL.

scholarly journals TOTAL PHENOLIC AND FLAVONOID CONTENT, ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL POTENTIAL OF PHLOMIS PUNGENS WILLD

10.23856/3713 ◽  
2020 ◽  
Vol 37 (6) ◽  
pp. 133-139
Author(s):  
Yehor Bazavluk ◽  
Vira Hamada ◽  
Nataliya Polish ◽  
Roksolana Konechna ◽  
Solomiia Mykytiuk ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Total Content ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Natural Plant ◽  
Ethanol Extracts ◽  
Antioxidant Effects ◽  
Phenolic And Flavonoid

The objective of the present study was to determine the total content of phenolics and flavonoids in ethanol extracts of Phlomis рungens Willd. The total phenolic content was estimated spectrophotometrically using Folin Ciocalteu method. The total flavonoid content was measured by aluminium chloride colorimetric assay. The antioxidant and antimicrobial effects of the extracts were investigated. The Phlomis рungens Willd can be regarded as a promising  natural plant source of antimicrobial and antioxidant effects with high potential for phytopreparations.

Evaluation of Antioxidant Activity and Total Phenols Content in Selected Spices

2017 ◽  
Vol 68 (7) ◽  
pp. 1429-1434 ◽  
Author(s):  
Gabriela Stanciu ◽  
Nicoleta Cristache ◽  
Simona Lupsor ◽  
Simona Dobrinas
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ocimum Basilicum ◽  
Extraction Time ◽  
Mentha Piperita ◽  
Total Phenolic ◽  
Thymus Vulgaris ◽  
Total Phenols ◽  
Phenolic Contents ◽  
Polyphenolic Content

The total phenols contents and antioxidant activities of alcoholic extractive solutions of Ocimum basilicum (basil), Thymus vulgaris (thyme), Mentha piperita (mint), Rosmarinus officinalis (rosemary), Sativa officinalis (sage), Artemisia dracunculus (tarragon) and Coriandrum sativum (coriander) were examined using Folin-Ciocalteu method and ACL method, respectively. Extractive alcoholic solutions of above mentioned seven spices were analyzed during a period of five months in order to determine the optimal extraction time when the polyphenolic content was at maximum. The highest total phenolic contents were registered for rosemary (608.37 mg GAE/100 g d.w.), sage (530.66 mg GAE/100g d.w.) and mint (511.87 mg GAE/100g d.w.) In general the optimal extraction time of the total phenolic content was reached after 3 months of maceration, except thyme, tarragon (after two months) and coriander (after one month). The antioxidant capacity ranged between 1410.1 nmols Trolox/g d.w. for rosemary and 4.2560 nmols Trolox/g d.w. for tarragon.

Influence of total phenolic content and total flavonoid content on the DPPH radical scavenging activity of Costus speciosus (Koen Ex. Retz.) Sm.

2015 ◽  
Vol 4 (5) ◽  
pp. 261-266
Author(s):  
M. Borkataky ◽  
B. B. Kakoti ◽  
L. R. Saikia
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Costus Speciosus ◽  
Phenolic And Flavonoid

  The focus of the present study was to determine the association of the anti-oxidant activity of Costus speciosus (Koen Ex. Retz.) Sm. with the total phe-nolic content and the total flavonoid content. The antioxidant activity of the extracts was quantified by diphenylpicrylhydrazyl (DPPH) assay. The total phenolic content was determined by the Folin-Ciocalteau method and the total flavonoid content was determined by the aluminium chloride method. Among the four extracts of the plant, the ethanol extract exhibited the high-est antioxidant activity and also the highest total phenolic and flavonoid con-tents. A strong correlation was observed between the antioxidant activity and the total phenolic and flavonoid contents. A statistical model was de-rived to explain this dependence and a non-linear association was observed between the antioxidant activity and the total flavonoid content.

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

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