scholarly journals Phenolic Compound Analysis and Pharmacological Screening of Vitex agnus-castus Functional Parts

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Assia Berrani ◽  
Ilias Marmouzi ◽  
Abdelhakim Bouyahya ◽  
Mourad Kharbach ◽  
Maha El Hamdani ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Agnus Castus ◽  
Promising Source

Vitex agnus-castus is a medicinal plant of the Verbenaceae family, widely used in traditional medicine. This study is aimed at investigating the functional variability of phenolic compounds in different parts (leaves, stems, flowers, roots, and seeds) of Vitex agnus-castus methanolic extracts and at assessing their in vitro antidiabetic, antioxidant, and antibacterial activities. The results of HPLC-DAD-QTOF-MS indicated the presence of 25 phenolic compounds with a remarkable variability between plant parts; high levels were registered in chlorogenic, vanillic, 3,4-dihydroxybenzoic, and 3-hydroxybenzoic acids; hesperidin; and luteolin. V. agnus castus fruits and stems presented higher antioxidant activities. The extracts inhibited the growth of five pathogenic bacteria with MIC values documented between 7.81 and 31.25 mg/mL. In vitro antihyperglycemic effect revealed higher effect in flowers (2921.84 μg/mL) and seeds (2992.75 μg/mL) against α-glucosidase and of leaves (2156.80 μg/mL) and roots (2357.30 μg/mL) against α-amylase. The findings of this showed that V. agnus castus is a promising source for antidiabetic bioactive compounds. However, further investigations regarding the evaluation of in vivo antidiabetic effects of these compounds are needed.

Phytochemical and Biological Activities of five Turanecio Hamzaoglu (Asteraceae) Species from Turkey

2017 ◽  
Vol 13 (1) ◽  
pp. 49-55
Author(s):  
Sevil Albayrak ◽  
Ahmet Aksoy ◽  
Abit Yasar ◽  
Lutfiye Yurtseven ◽  
Umit Budak
Keyword(s):  
Therapeutic Potential ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
In Vitro Assays ◽  
Methanolic Extracts ◽  
Promising Source

Objectives: In vitro biological activities of methanolic extracts of five Turanecio species have been studied. Materials and Methods: The phenolic compositions of the extracts were evaluated by the Folin- Ciocalteu assay and by HPLC analysis. Antioxidant activities were determined with two in vitro assays namely, DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging assay and phosphomolybdenum assay. The antimicrobial activities of the extracts were evaluated against 13 microorganisms. Results: T. hypochionaeus var. hypochionaeus was showed the highest DPPH inhibition with 88.84% at 100 μg·mL-1. All of the extracts were exerted high total antioxidant activities 128.00- 243.13 mg AAE g-1) and seem to be a promising source of natural antioxidants. The phenolic contents in the extracts varied from 26.17 to 60.99 mg·g-1 as gallic acid equivalent. Chlorogenic acid, caffeic acid and p- coumaric acid were the predominant constituents. The methanolic extracts revealed promising antibacterial activities against most bacteria. No activity was recorded against yeasts tested. Conclusion: The polyphenolic constituents appear to be responsible, at least in part, for the extract’s activities. The present study confirms that tested Turanecio species contains significant source of phenolics have antioxidant and antimicrobial activities and may have therapeutic potential.

scholarly journals HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF β-SITOSTEROL FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

2020 ◽  
pp. 162-165
Author(s):  
RIDHI JOSHI ◽  
RISHIKESH MEENA ◽  
PREETI MISHRA ◽  
VIDYA PATNI
Keyword(s):  
High Performance ◽  
Normal Phase ◽  
Leaf Sample ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.

scholarly journals Pharmacological potential of naturally occurring nonstarch polysaccharides (NSP)

2016 ◽  
Vol 4 (6) ◽  
pp. 307-310
Author(s):  
Dhwani T. Dave ◽  
◽  
Gaurang B. Shah ◽  
Keyword(s):  
Water Extract ◽  
Plant Metabolites ◽  
Secondary Plant Metabolites ◽  
Plant Parts ◽  
Pharmacological Potential ◽  
Crude Polysaccharide ◽  
Pharmacologically Active ◽  
Promising Source

Since ancient times, plants have been serving as a promising source of medicines and in recent times, extensive research has been made to isolate, characterize and screen a number of phytoconstituents/secondary plant metabolites for their pharmacological activities and safety in various disease models – both in vitro and in vivo. One such category of phytoconstituents is “polysaccharides”. These are found in different parts of the plant such as roots, leaves, stem and leaves and are extracted maximally in polar solvent – for eg. water extract of the afore mentioned plant parts contain the crude polysaccharide fraction. These are non-starch type in nature and can be a mixture of more than one type of polysaccharide too. In this review, an attempt has been made to discuss such therapeutically active plant polysaccharides in terms of their wide pharmacologically active profile along with methods to isolate and characterize them in brief.

scholarly journals Geographic Variation in the Chemical Composition and Antioxidant Properties of Phenolic Compounds from Cyclocarya paliurus (Batal) Iljinskaja Leaves

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2440 ◽  
Author(s):  
Yang Liu ◽  
Pei Chen ◽  
Mingming Zhou ◽  
Tongli Wang ◽  
Shengzuo Fang ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Geographic Origin ◽  
Cyclocarya Paliurus ◽  
Sampling Locations ◽  
Kaempferol Glycosides

Cyclocarya paliurus has been widely used as an ingredient in functional foods in China. However, the antioxidant properties of phenolic compounds and the effect of the plant origin remain unclear. The present study evaluated the geographical variation of this plant in term of its phenolic composition and antioxidant activities based on leaf materials collected from five regions. high-performance liquid chromatography (HPLC) analysis showed that there are three major components, quercetin-3-O-glucuronide, kaempferol-3-O-glucuronide, and kaempferol-3-O-rhamnoside, and their contents varied significantly among sampling locations. The investigated phenolic compounds showed substantial antioxidant activities, both in vitro and in vivo, with the highest capacity observed from Wufeng and Jinzhongshan. Correlation analysis revealed that quercetin and kaempferol glycosides might be responsible for the antioxidant activities. Our results indicate the importance of geographic origin, with sunny hours and temperature as the main drivers affecting the accumulation of C. paliurus phenolics and their antioxidant properties.

scholarly journals Design, Synthesis, and Biological Activity of Novel Myricetin Derivatives Containing Amide, Thioether, and 1,3,4-Thiadiazole Moieties

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3132 ◽  
Author(s):  
Xianghui Ruan ◽  
Cheng Zhang ◽  
Shichun Jiang ◽  
Tao Guo ◽  
Rongjiao Xia ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Xanthomonas Oryzae ◽  
Design Synthesis ◽  
Antiviral Activities ◽  
Potential Alternative ◽  
Better Than

A series of myricetin derivatives containing amide, thioether, and 1,3,4-thiadiazole moieties were designed and synthesized, and their antiviral and antibacterial activities were assessed. The bioassays showed that all the title compounds exhibited potent in vitro antibacterial activities against Xanthomonas citri (Xac), Ralstonia solanacearum (Rs), and Xanthomonas oryzae pv. Oryzae (Xoo). In particular, the compounds 5a, 5f, 5g, 5h, 5i, and 5l, with EC50 values of 11.5–27.3 μg/mL, showed potent antibacterial activity against Xac that was better than the commercial bactericides Bismerthiazol (34.7 μg/mL) and Thiodiazole copper (41.1% μg/mL). Moreover, the in vivo antiviral activities against tobacco mosaic virus (TMV) of the target compounds were also tested. Among these compounds, the curative, protection, and inactivation activities of 5g were 49.9, 52.9, and 73.3%, respectively, which were better than that of the commercial antiviral Ribavirin (40.6, 51.1, and 71.1%, respectively). This study demonstrates that myricetin derivatives bearing amide, thioether, and 1,3,4-thiadiazole moieties can serve as potential alternative templates for the development of novel, highly efficient inhibitors against plant pathogenic bacteria and viruses.

scholarly journals Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of Chuanminshen violaceum and Their Antioxidant Activities

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 266 ◽  
Author(s):  
Lin ◽  
Li ◽  
Wang ◽  
Liu ◽  
Yang ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Galacturonic Acid ◽  
Antioxidant Activities ◽  
Chemical Properties ◽  
Natural Antioxidants ◽  
Chemical Characteristics ◽  
Pectic Polysaccharides ◽  
Methanolic Extracts

The root of Chuanminshen violaceum is used as an important edible and medicinal plant in China. However, its leaves are generally considered byproducts, and therefore do not have a use. Thus, the phenolic compounds in the methanolic extracts (CVLMs) and the chemical characteristics of crude polysaccharides (CVLPs) from the leaves of C. violaceum and their in vitro antioxidant activities were explored. The results showed that chlorogenic acid and rutin were the major individual phenolic compounds in the leaves, which ranged from 1.22 ± 0.03 to 2.87 ± 0.04 mg/g DW, and from 2.25 ± 0.04 to 4.03 ± 0.05 mg/g DW, respectively. Meanwhile, the extraction yields of CVLPs from the leaves ranged from 4.73% to 5.41%. The CVLPs consisted of mannose, rhamnose, galacturonic acid, glucose, galactose, and arabinose, suggesting the existence of pectic polysaccharides. Furthermore, both CVLMs and CVLPs exhibited strong antioxidant activities. Chlorogenic acid and rutin were major contributors to the antioxidant activities of CVLMs, and the antioxidant activities of CVLPs were closely correlated to their α-1,4-D-galactosiduronic linkages. The results are beneficial for understanding the chemical properties and in vitro antioxidant activities of CVLMs and CVLPs. The leaves of C. violaceum have potential to be developed as natural antioxidants.

Phenolic-rich lychee (Litchi chinensis Sonn.) pulp extracts offer hepatoprotection against restraint stress-induced liver injury in mice by modulating mitochondrial dysfunction

2016 ◽  
Vol 7 (1) ◽  
pp. 508-515 ◽  
Author(s):  
Dongxiao Su ◽  
Ruifen Zhang ◽  
Cuilan Zhang ◽  
Fei Huang ◽  
Juan Xiao ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Liver Injury ◽  
Mitochondrial Dysfunction ◽  
Restraint Stress ◽  
Antioxidant Activities ◽  
Litchi Chinensis ◽  
Subtropical Fruit

The pulp from lychee, a tropical to subtropical fruit, contains large quantities of phenolic compounds and exhibits antioxidant activities both in vitro and in vivo.

scholarly journals Selective antimicrobial activity of maggots against pathogenic bacteria

2008 ◽  
Vol 57 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Domen Jaklič ◽  
Aleš Lapanje ◽  
Klemen Zupančič ◽  
Dragica Smrke ◽  
Nina Gunde-Cimerman
Keyword(s):  
Pathogenic Bacteria ◽  
Chronic Wounds ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Infected Wounds ◽  
Before And After ◽  
Treatment Of Wounds ◽  
Maggot Therapy

Maggot therapy, also known as biosurgery, is an ancient method for the healing of chronic infected wounds. Although clinicians have reported on the beneficial activities of the Lucilia sericata larvae that have been used for healing chronic wounds, the selectivity of this therapy against the different pathogenic micro-organisms that are found in chronic wounds has never been analysed. In the present study, we have investigated the in vitro activities of larval excreta/secreta both against selected bacterial strains that frequently occur in chronically infected wounds, and against bacteria isolated directly from the larvae and their excreta/secreta. Additionally, the antibacterial activities were investigated in in vivo studies, by comparing bacterial diversity in wounds before and after the application of L. sericata larvae. In conclusion, larval therapy is highly recommended, particularly for the treatment of wounds infected with Gram-positive bacteria, like Staphylococcus aureus, but less so for wounds infected with Gram-negative bacteria, especially Proteus spp. and Pseudomonas spp. strains. Bacteria from the genus Vagococcus were resistant to the maggot excreta/secreta.

Anabasis aretioides Coss. & Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties

2018 ◽  
Vol 30 (2) ◽  
pp. 251-257 ◽  
Author(s):  
Assia Berrani ◽  
Ilias Marmouzi ◽  
Mourad Kharbach ◽  
Abdelhakim Bouyahya ◽  
Maha El Hamdani ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Digestive Enzyme ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Ethnobotanical Survey ◽  
Traditional Use ◽  
Aerial Parts

Abstract Background Based on our previous ethnobotanical survey, the non-investigated Saharan plant Anabasis aretioides Coss. & Moq., growing in the region of Errachidia, was selected for pharmacological investigation. In Moroccan traditional medicine, A. aretioides is being used for diabetes treatment. Thus, the current work aims at evaluating the antidiabetic, antioxidant, and antibacterial activities of the plant in relation to the digestive tract. Methods The different parts of the plant (aerial parts, roots, seeds) were extracted with methanol (MeOH) and screened in enzymatic assays for their inhibitory potential against α-amylase and α-glucosidase, as well as antioxidant and antibacterial activities. Furthermore, the phenolic compounds were analyzed using HPLC-DAD-QTOF-MS. Results The MeOH extracts of A. aretioides aerial parts, roots, and seeds, respectively, inhibited α-amylase (IC50 of 3148.07 μg/mL, 2440.20 μg/mL, 3395.71 μg/mL) and α-glucosidase (IC50 of 2940.59 μg/mL, 3521.81 μg/mL, 3393.83 μg/mL). Moreover, compared to aerial parts and seeds, the plant roots exhibited higher antioxidant capacity and a potent reducing power. In resazurin microplate assay, the plant parts displayed a minimal inhibitory concentration (MIC) ranging from 7.81 mg/mL to 31.25 mg/mL. Chemical analysis revealed 25 phenolic compounds, with chlorogenic acid as the main phenolic compound in the aerial parts, hesperidin in roots, and quercitrin in seeds. Conclusion Anabasis aretioides cited for treatment of diabetes shows promising antioxidant and antibacterial properties, as well as an ability to inhibit digestive enzyme, including α-amylase and α-glucosidase. Thus, our results explain in part the traditional use of this Saharan medicine and open doors for further in vivo mechanistic and functional studies.

Identification and quantification of polyphenols from Cassia auriculata L. leaf, flower and flower bud using UPLC-QqQ-MS/MS

2021 ◽  
pp. 1-9
Author(s):  
Devanesan Arul Ananth ◽  
Vijayaraghavan Mahalakshmi ◽  
Thilagar Sivasudha ◽  
Liron Klipcan ◽  
Zipora Tietel
Keyword(s):  
Phenolic Compounds ◽  
Hydroxycinnamic Acids ◽  
Benzoic Acids ◽  
Flower Buds ◽  
Flower Bud ◽  
Plant Parts ◽  
Cassia Auriculata ◽  
Polyphenolic Composition

Abstract Cassia auriculata is an Ayurvedic medicinal herb, traditionally indicated for diabetes and hyperlipidemia. Several works have demonstrated its antioxidant, antidiabetic and anti-hyperlipidemic activity in vivo and in vitro. Nevertheless, only a few works have investigated its phytochemical composition, and specifically, the polyphenolic composition of the various plant parts that are traditionally used. In this work, the polyphenolic composition of C. auriculata leaves, flowers and flower buds were evaluated using UPLC-QqQ-MS/MS. Our results demonstrated the polyphenolic profile of C. auriculata plant parts. A total of five benzoic acids, four hydroxycinnamic acids, three flavonoids and two other phenolic compounds were identified and quantified. Our results show that in C. auriculata leaves, flavonoids were most abundant (4204 µg/g DW), while in flowers benzoic acids were the most prominent (3924 µg/g DW). Total benzoic acid contents ranged from 1580 to 3924 µg/g DW in leaf and flower, respectively. Hydroxycinnamic acids ranged from 404 µg/g DW in flower buds to 2623 µg/g DW in leaves. Flavonoids showed the highest contents in leaves, while the lowest levels were observed in flowers (2626 µg/g DW). The meaning of the results is discussed in light of the bioactivities of phenolic compounds, concomitant with C. auriculata reported medicinal bioactivities. To our knowledge, this is the first work to identify and quantify polyphenolic compounds in flower and bud of C. auriculata.

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