scholarly journals Posttreatment Downregulation of Type III Interferons in Patients with Acute Brucellosis

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Mehran Shokri ◽  
Oreinab Ghaffari Khonakdar ◽  
Mousa Mohammadnia-Afrouzi ◽  
Mahmoud Sadeghi-Haddad-Zavareh ◽  
Amirhossein Hasanpour ◽  
...  
Keyword(s):  
Clinical Studies ◽  
Standard Therapy ◽  
Human Brucellosis ◽  
The Novel ◽  
Northern Iran ◽  
Type Iii ◽  
Before And After ◽  
Acute Form

There is a limited number of clinical studies on interferon (IFN) levels in human brucellosis. The novel group of interferons, type III interferons, which consists of four IFN-λ (lambda) molecules called IFN-λ1 or interleukin-29 (IL-29), IFN-λ2 or IL-28A, IFN-λ3 or IL-28B, and IFN-λ4, is not fully known. This study is one of the first studies of IL-28A and IL-29 levels in brucellosis cases at the end of their treatment course. A total of 33 acute brucellosis patients were included in this study. We considered changes in the levels of IL-28A and IL-29 in cases with acute brucellosis before and after treatment with standard therapy that referred to the Ayatollah Rohani Hospital in Babol, northern Iran. Of 33 included patients, 22 (66.6%) were males, and 11 (33.4%) were females. The range of patients’ age was 49.21 ± 17.70 years. Serum IL-29 and IL-28A (acute form: 56.4 ± 30.32  pg/mL and 48.73 ± 27.72  pg/mL, respectively, and posttreatment: 40.15 ± 20.30  pg/mL and 38.79 ± 22.66  pg/mL, respectively) levels were elevated significantly in acute brucellosis than after treatment ( p < 0.05 ). These findings indicate that considering biomarker levels in brucellosis patients may indicate the chronicity of infection. In conclusion, we suggest that IL-29 and IL-28A levels may be valuable biomarkers for follow-up patients with brucellosis.

scholarly journals Post-treatment downregulation of type III interferons in patients with acute Brucellosis

2021 ◽  
Author(s):  
Mehran Shokri ◽  
Oreinab Ghaffari Khonakdar ◽  
Mousa Mohammadnia-Afrouzi ◽  
Mahmoud Sadeghi-Haddad-Zavareh ◽  
Amirhossein Hasanpour ◽  
...  
Keyword(s):  
Clinical Studies ◽  
Standard Therapy ◽  
Post Treatment ◽  
Human Brucellosis ◽  
The Novel ◽  
Northern Iran ◽  
Type Iii ◽  
Before And After ◽  
Acute Form

There is a limited number of clinical studies on interferon (IFN) levels in human brucellosis. The novel group of interferons, type III interferons, consists of four IFN-λ (lambda) molecules called IFN-λ1 or Interleukin-29 (IL-29), IFN-λ2 or IL-28A, IFN-λ3 or IL-28B, and IFN-λ4, are not fully known. This study is one of the first studies of IL-28A and IL-29 levels in Brucellosis cases at the end of their treatment course. A total of 33 acute Brucellosis patients were included in this study. We considered changes in the levels of IL-28A and IL-29 in cases with acute brucellosis before and after treatment with standard therapy that referred to the Ayatollah Rohani Hospital in Babol, Northern Iran. Serum IL-29 and IL-28A (acute form: 56.4 +- 30.32 and 48.73 +- 27.72, respectively, and post-treatment: 40.15 +- 20.30 and 38.79 +- 22.66, respectively) levels were elevated significantly in acute brucellosis than after treatment (p ≤ 0.05). These findings indicate that considering biomarker levels in Brucellosis patients may be indicative of the chronicity of infection. In conclusion, we suggest that IL-29 and IL-28A levels may be valuable biomarkers for follow-up patients with brucellosis.

Low Immunogenicity to Romiplostim in Clinical Studies with ITP Subjects.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3425-3425 ◽  
Author(s):  
Vibha Jawa ◽  
Martha Hokom ◽  
Jenny Hu ◽  
Yao Zhuang ◽  
Dietmar Berger ◽  
...  
Keyword(s):  
Clinical Studies ◽  
Neutralizing Antibodies ◽  
Single Chain ◽  
Serum Samples ◽  
Binding Domains ◽  
C Terminus ◽  
Before And After ◽  
Immunogenicity Assessment ◽  
Covalently Linked

Abstract Romiplostim, a member of the thrombopoietin (TPO) mimetic class, is an Fc-peptide fusion protein (peptibody) that activates intracellular transcriptional pathways leading to increased platelet production via the TPO receptor (also known as cMpl). The peptibody molecule contains two identical single-chain subunits each consisting of human immunoglobulin IgG1 Fc domain, covalently linked at the C-terminus to a peptide containing two TPO receptor-binding domains. Due to the general concern regarding the immunogenic potential for all therapeutic proteins and the specific concern for monitoring antibodies capable of neutralizing thrombopoietin (TPO), an extensive immunogenicity assessment program was developed to support romiplostim. Romiplostim has been engineered to have no amino acid sequence homology to endogenous TPO. A low theoretical risk of developing conformational antibodies that cross-react against TPO exists. This risk was addressed by using an immunogenicity assessment strategy that relied upon a surface plasmon resonance based biosensor immunoassay using the Biacore 3000 capable of simultaneously monitoring antibodies that bind to romiplostim, TPO, or the active peptide portion of romiplostim (TMP). Samples that tested positive for binding antibodies in the Biacore immunoassay were then tested in the definitive functional biological assay to identify any antibodies capable of neutralizing the biological effect of romiplostim or TPO. Serum samples from 236 actively treated subjects were obtained both before and after exposure to romiplostim and were tested for romiplostim and TPO antibodies. In baseline samples, seventeen subjects (7.1%) tested romiplostim antibody positive and 12 subjects (5.1%) tested TPO antibody positive for pre-existing binding antibodies. After romiplostim exposure, twenty-five out of 236 (10.5%) subjects with ITP developed binding antibodies against romiplostim (inclusive of antibodies to both peptide and the whole molecule) and 12 out of 236 (5.1%) subjects with ITP developed binding antibodies against TPO. The antibodies that developed against romiplostim did not cross react with TPO and the antibodies that developed against TPO did not cross react with romiplostim. The incidence of anti-romiplostim neutralizing antibodies among 236 subjects with ITP who were treated with romiplostim across 10 clinical studies was 0.4% (1 out of 236). No cases of anti-TPO neutralizing antibodies were detected in romiplostim treated samples. In conclusion, after thorough immunogenicity assessment of all subjects treated with romiplostim using sensitive methods to detect binding and neutralizing antibodies, only one subject was found positive for the presence of antibodies capable of neutralizing romiplostim that was negative at the time of follow up 4 months later. As expected, none of the subjects treated were positive for antibodies capable of neutralizing TPO. No clinical sequelae were observed in association to the presence of antibodies.

scholarly journals Vascular Response on a Novel Fibrin-Based Coated Flow Diverter

2021 ◽  
Author(s):  
Ruben Mühl-Benninghaus ◽  
Frederik Fries ◽  
Mara Kießling ◽  
Toshiki Tomori ◽  
Stefanie Krajewski ◽  
...  
Keyword(s):  
Animal Model ◽  
Flow Diverter ◽  
Endothelial Cell Proliferation ◽  
The Novel ◽  
Nano Coating ◽  
Before And After ◽  
Proliferation In Vitro

Abstract Purpose Due to thromboembolic complications and in-stent-stenosis after flow diverter (FD) treatment, the long-term use of dual antiplatelet treatment (DAPT) is mandatory. The tested nano-coating has been shown to reduce material thrombogenicity and promote endothelial cell proliferation in vitro. We compared the biocompatibility of coated (Derivo Heal) and non-coated (Derivo bare) FDs with DAPT in an animal model. Methods Derivo® bare (n = 10) and Derivo® Heal (n = 10) FD were implanted in the common carotid arteries (CCAs) of New Zealand white rabbits. One additional FD, alternately a Derivo bare (n = 5) or Derivo Heal (n = 5), was implanted in the abdominal aorta (AA) for assessment of the patency of branch arteries. Histopathological examinations were performed after 28 days. Angiography was performed before and after FD implantation and at follow-up. Results Statistical analysis of the included specimens showed complete endothelialization of all FDs with no significant differences in neointima thickness between Derivo® bare and Derivo® Heal (CCA: p = 0.91; AA: p = 0.59). A significantly reduced number of macrophages in the vessel wall of the Derivo Heal was observed for the CCA (p = 0.02), and significantly reduced fibrin and platelet deposition on the surface of the Derivo Heal was observed for the AA. All branch arteries of the stented aorta remained patent. Conclusion In this animal model, the novel fibrin-based coated FD showed a similar blood and tissue compatibility as the non-coated FD.

scholarly journals Hybrid Robot-Assisted Gait Training for Motor Function in Subacute Stroke: A Single-Blind Randomized Controlled Trial

2021 ◽  
Author(s):  
Yen-Nung Lin ◽  
Shih-Wei Huang ◽  
Yi-Chun Kuan ◽  
Hung-Chou Chen ◽  
Wen-Shan Jian ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Lower Extremity ◽  
Controlled Trial ◽  
Gait Training ◽  
The Novel ◽  
Robot Assisted ◽  
Randomized Controlled ◽  
Subacute Stroke ◽  
Before And After

Abstract Background:Robot-assisted gait training (RAGT) is a practical treatment adjunctive to conventional rehabilitation to provide high-intensity repetitive training for patients with stroke. The robot models designed are usually either the end-effector type or the exoskeleton type. We developed a novel hybrid RAGT system that combines the advantages of both types.Objective:This randomized controlled trial (RCT) evaluated whether the novel RAGT system was beneficial and assessed long-term effects (at the 3-month follow-up) for nonambulatory patients with subacute stroke.Methods:This trial recruited 40 patients with subacute stroke who were equally randomized to receive conventional rehabilitation or 15 add-on RAGT sessions. We assessed lower-extremity motor function, balance, and gait performance by using the following tools: active range of motion (AROM), manual muscle test (MMT), the Fugl-Meyer Assessment (FMA) lower-extremity subscale (FMA-LE) and total (FMA-total), Postural Assessment Scale for Stroke (PASS), Berg Balance Scale (BBS), Tinetti Performance Oriented Mobility Assessment (POMA) balance and gait subscores, and the 3- and 6-m walking speed and Timed Up and Go (TUG) tests. These measurements were performed before and after the intervention and at the 3-month follow-up.Results:Both groups demonstrated significant within-group changes in the AROM, MMT, FMA-LE, FMA-total, PASS, BBS, POMA, TUG, and 3- and 6-m walking speeds before and after intervention and at the 3-month follow-up (p < 0.05). Only FMA-LE (p = 0.014) and total (p = 0.002) scores differed significantly between groups (RAGT vs. control).Conclusion:Although the novel hybrid RAGT was beneficial, no powerful evidence to supported its effectiveness relative to the control group in substantial leg dysfunction after stroke.Trial Registration: The study was registered with an International Standard Randomized Controlled Trial Number, ISRCTN, ISRCTN15088682. Registered 16 September 2016, Retrospectively registered https://www.isrctn.com/ISRCTN15088682

Melatih Kepemimpinan Perangkat Desa Kebonharjo Kulonprogo: Membangun Sikap Anti Korupsi

2018 ◽  
Vol 1 ◽  
pp. 107
Author(s):  
Adi Heryadi ◽  
Evianawati Evianawati
Keyword(s):  
Transformational Leadership ◽  
Data Collection ◽  
Paired Samples ◽  
Significant Difference ◽  
Before And After ◽  
Post Test ◽  
Sample Test ◽  
Corruption Perception ◽  
Measuring Tool

This study aims to prove whether transformational leadership training is effective for building anti-corruption attitudes of villages in Kebonharjo village, subdistrict Samigaluh Kulonprogo. This research is an experimental research with one group pre and posttest design.Subject design is 17 people from village of 21 candidates registered. Measuring tool used in this research is the scale of anti-corruption perception made by the researcher referring to the 9 anti-corruption values with the value of reliability coefficient of 0.871. The module used as an intervention made by the researcher refers to the transformational leadership dimension (Bass, 1990). The data collected is analyzed by statistical analysis of different test Paired Sample Test. Initial data collection results obtained sign value of 0.770 which means> 0.05 or no significant difference between anti-corruption perception score between before and after training. After a period of less than 1 (one) month then conducted again the measurement of follow-up of the study subjects in the measurement again using the scale of anti-corruption perception. The results of the second data collection were analysed with Paired Samples Test and obtained the value of 0.623 sign meaning p> 0.05 or no significant difference between post test data with follow-up data so that the hypothesis of this study was rejected.

More Alike Than Unalike: Cross-language Similarity Influences Novel Word Lexicalization in Bilingual Individuals

2020 ◽  
Author(s):  
Pauline Palma ◽  
Marie-France Marin ◽  
k onishi ◽  
Debra Titone
Keyword(s):  
Lexical Decision ◽  
Lexical Decision Task ◽  
Decision Task ◽  
The Novel ◽  
Lexical Representations ◽  
Before And After ◽  
Word Forms ◽  
Explicit Recognition ◽  
Novel Word Learning ◽  
Cross Language

Although several studies have focused on novel word learning and consolidation in native (presumably monolingual) speakers, less is know about how bilinguals add novel words to their mental lexicon. Here, we trained 33 English-French bilinguals on novel word-forms that were neighbors to “hermit” English words (i.e., words with no existing neighbors). Importantly, these English words varied in terms of orthographic overlap with their French translation equivalent (i.e., cognates vs. noncognates). We measured explicit recognition of the novel neighbors and the interaction between novel neighbors and English words through a lexical decision task, both before and after a sleep interval. In the lexical decision task, we found evidence of immediate facilitation for English words with novel neighbors, and evidence of competition after a sleep interval for cognate words only. These results suggest that higher quality of existing lexical representations predicts an earlier onset for novel word lexicalization.

SUBLINGUAL VITAMIN D3 DRUG THERAPY IN VITAMIN D DEFICIENCY PATIENTS AT PRAVARA RURAL HOSPITAL

2019 ◽  
pp. 18-20
Author(s):  
Sanjeeva Kumar Goud T ◽  
Rahul Kunkulol
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Vitamin D3 ◽  
Serum Vitamin ◽  
Cross Sectional ◽  
Serum Vitamin D ◽  
Significant Difference ◽  
Before And After

The present study was aimed to study the effect of Sublingual Vitamin D3 on Serum Vitamin D level in Vitamin D deficiency patients. This was a cross-sectional and interventional study. All the Vitamin D deficiency patients of age 18-60years and either gender, willing to participate in the study were included. Patients who had greater than 20 ng/ml were excluded from the study. The total number of participants in our study was 200, out of these 111 males and 89 females, the mean age in our study was 51.07 ± 7.39Yrs. All volunteers were given sublingual vitamin D3 (60,000IU) in six doses every fifteen days of follow up for 3 months. The subject’s serum 25(OH)D levels were estimated before and after the treatment of sublingual vitamin D3. There was a statistically significant difference in serum vitamin D3 level before 16.61±6.71 ng/ml and after 35.80±7.80 ng/ml after treatment with Sublingual Vitamin D3. Six doses of 60,000IU of Vitamin D3 sublingual route having improved the role of serum 25(OH)D levels in the treatment of Vitamin D3 deficiency patients.Keywords: Vitamin D3; Sublingual route

A CRITICAL ASSESSMENT OF THE NOVEL «ASSEMBLY HALL» BY HERMANN KANT IN THE REUNIFIED GERMANY (AFTER 1991)

2020 ◽  
pp. 144-152
Author(s):  
Rimma Gurevich
Keyword(s):  
Critical Assessment ◽  
The Novel ◽  
Aesthetic Appreciation ◽  
Party Position ◽  
Creative Personality ◽  
Before And After

H. Kant’s novel received a high literary and aesthetic appreciation in criticism and wide recognition by readers. Criticism (before and after the unification of Germany) concerns mainly one aspect viz. authenticity of the events depicted in the novel and the charcters’ images. Opponents argue that Kant’s ideological views, his consistent socialist and party position have prompt him to embellish reality, create simulacra, and the idyllic world of socialist Biedermeyer. The article shows that these assessments ignore the nature of his talent, especially his creative personality peculiarities such as journalistic orientation of the motivated «political» person and writer.

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