Wound Healing Activities of Hydromethanolic Crude Extract and Solvent Fractions of Bersama abyssinica Leaves in Mice

Background. Bersama abyssinica leaves are traditionally used for management of wounds in several communities of Ethiopia, despite no scientifically approved studies done on wound healing. Our study planned to work out the wound healing effects of B. abyssinica leaves extract in mice. Methods. B. abyssinica leaves were extracted with 80% hydromethanol and fractioned with chloroform, hexane, and water. The acute dermal toxicity of the crude extract was evaluated in mice. The crude extract formulated at 5% and 10% w/w ointment was investigated in excision, incision, and burn wound models and solvent fractions in the excision model using simple ointment (negative control) and nitrofurazone 0.2% w/v (positive control). We evaluated histopathological analysis, wound contraction rate, complete epithelialization period, and skin durability. One-way ANOVA followed by the post hoc Tukey HSD test with IBM SPSS software version 23.0 was used for data analysis, and p < 0.05 was considered statistically significant. Results. Hydromethanolic crude extract produced 5% (99.5%) and 10% (100%) wound contraction on the 16th day of the treatment and 5% (18.8) and 10% (28.2) percent reduction in the epithelization period on the excision wound healing model. Hydromethanolic crude extract produced 5% (47.5) and 10% (61.17) percent durability on the incision wound healing model. Hydromethanolic crude extract produced 5% (99.82%) and 10% (100%) wound contraction on the 20th day of treatment and 5% (13%) and 10% (21.7%) reduction in the epithelization period on the burn wound healing model. The chloroform fraction produced 5% (90.17%) and 10% (91.01%), hexane fraction produced 5% (85.81%) and 10% (86.78%), and aqueous fraction produced 5% (99.17%) and 10% (99.38%) wound contraction on the 14th day of the treatment and 5% (18.8) and 10% (28.2) percent reduction in the epithelization period on the excision wound healing model. Both hydromethanolic crude extract and solvent fractions at 5% and 10% (w/w) were significant ( p < 0.001) compared with negative control. Conclusion. The results of this study showed that both 5% w/w and 10% w/w of 80% hydromethanolic crude extract and solvent fractions of B. abyssinica leaves have wound healing effects.

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512 Use of Artificial Dermis as a Wound Bed for Cultured Keratinocyte Sheet Cultured Using Temperature Response Dishes in an Ovine Burn Wound Healing Model

Journal of Burn Care & Research ◽

10.1093/jbcr/irz013.402 ◽

2019 ◽

Vol 40 (Supplement_1) ◽

pp. S231-S232

Author(s):

Y Niimi ◽

S Alharbi ◽

S Fukuda ◽

V L Popov ◽

K Liu ◽

...

Keyword(s):

Wound Healing ◽

Temperature Response ◽

Burn Wound ◽

Burn Wound Healing ◽

Artificial Dermis ◽

Wound Healing Model ◽

Healing Model

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A Burn Wound Healing Model in the Hairless Descendant of the Mexican Hairless Dog

Journal of Burn Care & Rehabilitation ◽

10.1097/00004630-199707000-00005 ◽

1997 ◽

Vol 18 (4) ◽

pp. 306-312 ◽

Cited By ~ 18

Author(s):

Hajime Matsumura ◽

Naoki Yoshizawa ◽

Tohru Kimura ◽

Katsueki Watanabe ◽

Nicole S. Gibran ◽

...

Keyword(s):

Wound Healing ◽

Burn Wound ◽

Burn Wound Healing ◽

Wound Healing Model ◽

Healing Model

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Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

Experimental Cell Research ◽

10.1016/j.yexcr.2013.09.016 ◽

2014 ◽

Vol 320 (1) ◽

pp. 79-91 ◽

Cited By ~ 14

Author(s):

Nina Tandon ◽

Elisa Cimetta ◽

Aranzazu Villasante ◽

Nicolette Kupferstein ◽

Michael D. Southall ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Wound Healing ◽

Reactive Oxygen ◽

Dermal Fibroblasts ◽

Oxygen Species ◽

Human Dermal Fibroblasts ◽

Wound Healing Model ◽

Healing Model

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Transcriptomic Analysis of a Diabetic Skin-Humanized Mouse Model Dissects Molecular Pathways Underlying the Delayed Wound Healing Response

Genes ◽

10.3390/genes12010047 ◽

2020 ◽

Vol 12 (1) ◽

pp. 47

Author(s):

Carlos León ◽

Francisco García-García ◽

Sara Llames ◽

Eva García-Pérez ◽

Marta Carretero ◽

...

Keyword(s):

Wound Healing ◽

Functional Enrichment ◽

Preclinical Model ◽

Published Data ◽

Diabetic Patients ◽

Ulcer Formation ◽

Histological Features ◽

Depth Analysis ◽

Wound Healing Model ◽

Healing Model

Defective healing leading to cutaneous ulcer formation is one of the most feared complications of diabetes due to its consequences on patients’ quality of life and on the healthcare system. A more in-depth analysis of the underlying molecular pathophysiology is required to develop effective healing-promoting therapies for those patients. Major architectural and functional differences with human epidermis limit extrapolation of results coming from rodents and other small mammal-healing models. Therefore, the search for reliable humanized models has become mandatory. Previously, we developed a diabetes-induced delayed humanized wound healing model that faithfully recapitulated the major histological features of such skin repair-deficient condition. Herein, we present the results of a transcriptomic and functional enrichment analysis followed by a mechanistic analysis performed in such humanized wound healing model. The deregulation of genes implicated in functions such as angiogenesis, apoptosis, and inflammatory signaling processes were evidenced, confirming published data in diabetic patients that in fact might also underlie some of the histological features previously reported in the delayed skin-humanized healing model. Altogether, these molecular findings support the utility of such preclinical model as a valuable tool to gain insight into the molecular basis of the delayed diabetic healing with potential impact in the translational medicine field.

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EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i3.23223 ◽

2018 ◽

Vol 11 (3) ◽

pp. 152

Author(s):

Sujith V ◽

Poornima G ◽

Balaji O ◽

Bairy Kl ◽

Praveen K ◽

...

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Chronic Wounds ◽

Statistical Significance ◽

Silver Sulfadiazine ◽

Histopathological Analysis ◽

Burn Wound ◽

Excision Wound ◽

Burn Wounds ◽

Wound Model

Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

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EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

Kongunadu Research Journal ◽

10.26524/krj17 ◽

2014 ◽

Vol 1 (1) ◽

pp. 73-77

Author(s):

Uma Maheswari P ◽

Shalimol A ◽

Arumugasamy A ◽

Udhaya Sankar M.R

Keyword(s):

Wound Healing ◽

Dry Weight ◽

Wound Contraction ◽

Albino Rats ◽

Standard Drug ◽

Excision Wound ◽

Wound Model ◽

Wistar Albino Rats ◽

Incision Wound ◽

Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

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