scholarly journals PAF and Haematopoiesis. I. 5-Fluoro-Uracil Induces PAF Production in Haematopoietic Organs of Rats

1994 ◽  
Vol 3 (1) ◽  
pp. 23-25 ◽  
Author(s):  
Y. Denizot ◽  
V. Praloran
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Platelet Activating Factor ◽  
Femoral Bone ◽  
Before And After ◽  
Apoptotic Effects ◽  
First Time ◽  
Femoral Bone Marrow

Haematopoietic organs of rats were examined for the presence of platelet-activating factor (PAF) and acetylhydrolase before and after treatment with 5-fluoro-uracil (5-FU) (200 mg/kg) a chemotherapeutic compound with apoptotic effects. PAF was reported in thymus, spleen and femoral bone marrow of rats with or without 5-FU. Although acetylhydrolase activity in organs was not affected by 5-FU treatment, elevated levels of PAF were observed in thymus and spleen. For the first time PAF is reported in haematopoietic organs of rats, strengtheningin vitrodata suggesting its role in the apoptotic processes in thymus, in the modulation of the immune response, and in the regulation of haematopoiesis.

scholarly journals Acetabular and femoral bone marrow in patients with hip osteoarthrosis: Cellular landscape of immune and stromal compartments

Bone Reports ◽  
2021 ◽  
Vol 14 ◽  
pp. 100844
Author(s):  
Drenka Trivanovic ◽  
Janek Hader ◽  
Maximilian Leucht ◽  
Theresa Kreuzahler ◽  
Bianca Schlierf ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Femoral Bone ◽  
Femoral Bone Marrow

scholarly journals Arginase Activity in Eisenia andrei Coelomocytes: Function in the Earthworm Innate Response

2021 ◽  
Vol 22 (7) ◽  
pp. 3687
Author(s):  
Joanna Homa ◽  
Alina Klosowska ◽  
Magdalena Chadzinska
Keyword(s):  
Immune Response ◽  
Wound Healing ◽  
Arginase Activity ◽  
Eisenia Andrei ◽  
Heavy Metals Ions ◽  
First Time ◽  
Important Marker

Arginase is the manganese metalloenzyme catalyzing the conversion of l-arginine to l-ornithine and urea. In vertebrates, arginase is involved in the immune response, tissue regeneration, and wound healing and is an important marker of alternative anti-inflammatory polarization of macrophages. In invertebrates, data concerning the role of arginase in these processes are very limited. Therefore, in the present study, we focused on the changes in arginase activity in the coelomocytes of Eisenia andrei. We studied the effects of lipopolysaccharide (LPS), hydrogen peroxide (H2O2), heavy metals ions (e.g., Mn2+), parasite infection, wound healing, and short-term fasting (5 days) on arginase activity. For the first time in earthworms, we described arginase activity in the coelomocytes and found that it can be up-regulated upon in vitro stimulation with LPS and H2O2 and in the presence of Mn2+ ions. Moreover, arginase activity was also up-regulated in animals in vivo infected with nematodes or experiencing segment amputation, but not in fasting earthworms. Furthermore, we confirmed that the activity of coelomocyte arginase can be suppressed by l-norvaline. Our studies strongly suggest that similarly to the vertebrates, also in the earthworms, coelomocyte arginase is an important element of the immune response and wound healing processes.

scholarly journals FEMORAL BONE MARROW BIOPSY IN THE ALBINO RAT

Blood ◽  
1948 ◽  
Vol 3 (3) ◽  
pp. 292-294 ◽  
Author(s):  
D. G. CAMERON ◽  
G. M. WATSON
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Biopsy ◽  
Femoral Bone ◽  
Albino Rat ◽  
Femoral Bone Marrow ◽  
Differential Counts

Abstract A technic for repeated femoral bone marrow biopsy in the rat is detailed. Differential counts confirmed the distribution of the cellular elements as described by other authors.

scholarly journals Influence of Cobalt, Sheep Erythropoietin and Several Hormones on Erythropoiesis in Bone Marrows of Isolated Perfused Hind Limbs of Dogs

Blood ◽  
1964 ◽  
Vol 23 (1) ◽  
pp. 87-98 ◽  
Author(s):  
JAMES W. FISHER ◽  
B. L. ROH ◽  
CHARLES COUCH ◽  
W. O. NIGHTINGALE
Keyword(s):  
Bone Marrow ◽  
Growth Hormone ◽  
Erythroid Cell ◽  
Femoral Bone ◽  
Perfusion System ◽  
Cell Counts ◽  
Hind Limbs ◽  
Erythroid Hyperplasia ◽  
Dog Blood ◽  
Femoral Bone Marrow

Abstract A technic has been developed for studying the femoral bone marrow of the dog by perfusing the isolated hind limb. With the use of this method, bone marrows were perfused for 4-6 hours with donor dog blood alone and blood containing sheep erythropoietin, cobalt, growth hormone, hydrocortisone, 3,5,3'-triiodothyronine or testosterone. Significant increases were seen in nucleated erythroid cell counts within femoral marrows perfused with blood containing sheep erythropoietin and cobalt. A slight increase was also noted in the bone marrows from limbs in which growth hormone was added to the perfusion system. It may be concluded from these experiments that erythropoietin, cobalt and growth hormone are capable of stimulating erythroid hyperplasia in the bone marrow directly.

scholarly journals The Diverse Potential of Gluten from Different Durum Wheat Varieties in Triggering Celiac Disease: A Multilevel In Vitro, Ex Vivo and In Vivo Approach

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3566
Author(s):  
Federica Gaiani ◽  
Sara Graziano ◽  
Fatma Boukid ◽  
Barbara Prandi ◽  
Lorena Bottarelli ◽  
...  
Keyword(s):  
Immune Response ◽  
Celiac Disease ◽  
Durum Wheat ◽  
Ex Vivo ◽  
Diet Group ◽  
Control Group ◽  
Wheat Varieties ◽  
Before And After

The reasons behind the increasing prevalence of celiac disease (CD) worldwide are still not fully understood. This study adopted a multilevel approach (in vitro, ex vivo, in vivo) to assess the potential of gluten from different wheat varieties in triggering CD. Peptides triggering CD were identified and quantified in mixtures generated from simulated gastrointestinal digestion of wheat varieties (n = 82). Multivariate statistics enabled the discrimination of varieties generating low impact on CD (e.g., Saragolla) and high impact (e.g., Cappelli). Enrolled subjects (n = 46) were: 19 healthy subjects included in the control group; 27 celiac patients enrolled for the in vivo phase. Celiacs were divided into a gluten-free diet group (CD-GFD), and a GFD with Saragolla-based pasta group (CD-Sar). The diet was followed for 3 months. Data were compared between CD-Sar and CD-GFD before and after the experimental diet, demonstrating a limited ability of Saragolla to trigger immunity, although not comparable to a GFD. Ex vivo studies showed that Saragolla and Cappelli activated immune responses, although with great variability among patients. The diverse potential of durum wheat varieties in triggering CD immune response was demonstrated. Saragolla is not indicated for celiacs, yet it has a limited potential to trigger adverse immune response.

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