Antiapoptotic Activity of Autocrine Interleukin-22 and Therapeutic Effects of Interleukin-22-Small Interfering RNA on Human Lung Cancer Xenografts

Our previous studies have shown Leptomycin B (LMB) is a promising antilung cancer drug. Epigallocatechin-3-gallate (EGCG) has antitumor properties but a debatable clinical application. The objective of this study is to evaluate the combination therapeutic effect of LMB and EGCG and its molecular mechanisms in human lung cancer A549 cells. Increased cytotoxicity was observed in LMB+EGCG-treated cells compared to LMB-treated cells. Elevated ROS was maximized 2 h after treatment, and LMB+EGCG-treated cells had higher ROS levels compared to LMB. N-Acetyl-L-cysteine (NAC) studies confirmed the oxidative role of LMB and/or EGCG treatment. In comparison to the control, CYP3A4, SOD, GPX1, and p21 mRNA expression levels were increased 7.1-, 2.0-, 4.6-, and 13.1-fold in LMB-treated cells, respectively, while survivin was decreased 42.6-fold. Additionally, these increases of CYP3A4, SOD, and GPX1 were significantly reduced, while p21 was significantly increased in LMB+EGCG-treated cells compared to LMB-treated cells. The qRT-PCR results for p21 and survivin were further confirmed by Western blot. Our study first shows that LMB produces ROS and is possibly metabolized by CYP3A4, GPX1, and SOD in A549 cells, and combination treatment of LMB and EGCG augments LMB-induced cytotoxicity through enhanced ROS production and the modulation of drug metabolism and p21/survivin pathways.

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The Effect of Dark Septate Endophytic Fungi on Mahonia oiwakensis

Plants ◽

10.3390/plants10081723 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1723

Author(s):

Lei-Chen Lin ◽

Yin-Ling Tan ◽

Wan-Rou Lin ◽

Kuo-Lung Ku ◽

Shang-Tse Ho

Keyword(s):

Lung Cancer ◽

Growth Response ◽

Human Lung ◽

Growth Promotion ◽

Human Lung Cancer ◽

Composition Analysis ◽

Therapeutic Effects ◽

Fresh Weight ◽

Chemical Composition Analysis ◽

Total Fresh Weight

This is the first study to discuss the effects of dark septate endophytes (DSE) on the growth promotion and berberine concentration in Mahonia oiwakensis, whose extract (MOE) has been suggested to have potential therapeutic effects against human lung cancer. First, as per phylogenetic analysis, the strains were divided into four groups: CkDB2, CkDB5, MoAL2 and MoAL5. All of these were DSEs, which could form microsclerotia in M. oiwakensis. The growth response experiment revealed that inoculation of the plant with MoAL5 and CkDB5 promoted an increase in the total fresh weight of the seedlings. Chemical composition analysis showed that seedlings inoculated with CkDB5 had the highest berberine concentration. These results showed that some DSEs have the ability to promote growth and induce phytochemical responses in the host plant.

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Suppression of Type 1 Insulin-like Growth Factor Receptor Expression by Small Interfering RNA Inhibits A549 Human Lung Cancer Cell Invasion in vitro and Metastasis in Xenograft Nude Mice

Acta Biochimica et Biophysica Sinica ◽

10.1111/j.1745-7270.2007.00257.x ◽

2007 ◽

Vol 39 (2) ◽

pp. 137-147 ◽

Cited By ~ 16

Author(s):

Jianfang QIAN ◽

Aiqiang DONG ◽

Minjian KONG ◽

Zhiyuan MA ◽

Junqiang FAN ◽

...

Keyword(s):

Cell Invasion ◽

Small Interfering Rna ◽

Human Lung ◽

Growth Factor Receptor ◽

Receptor Expression ◽

Human Lung Cancer ◽

Lung Cancer Cell ◽

Interfering Rna

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The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells

Journal of Amino Acids ◽

10.1155/2013/251398 ◽

2013 ◽

Vol 2013 ◽

pp. 1-13 ◽

Cited By ~ 13

Author(s):

Julio R. Fernández Massó ◽

Brizaida Oliva Argüelles ◽

Yelaine Tejeda ◽

Soledad Astrada ◽

Hilda Garay ◽

...

Keyword(s):

Lung Cancer ◽

Tumor Cells ◽

Mechanism Of Action ◽

Human Lung ◽

Anticancer Therapy ◽

Human Lung Cancer ◽

Potential Candidate ◽

Human Lung Cancer Cells ◽

Cell Penetrating ◽

Antiapoptotic Activity

We have demonstrated that the peptide L-2 designed from an alanine scanning of the Limulus-derived LALF32-51 region is a potential candidate for the anticancer therapy and its cell-penetrating capacity is an associated useful property. By the modification in the primary structure of L-2, a second-generation peptide (CIGB-552) was developed. However, the molecular mechanism underlying its cytotoxic activity remains partially unknown. In this study, it was shown that CIGB-552 increases the levels of COMMD1, a protein involved in copper homeostasis, sodium transport, and the NF-κB signaling pathway. We found that CIGB-552 induces ubiquitination of RelA and inhibits the antiapoptotic activity regulated by NF-κB, whereas the knockdown of COMMD1 blocks this effect. We also found that CIGB-552 decreases the antioxidant capacity and induces the peroxidation of proteins and lipids in the tumor cells. Altogether, this study provides new insights into the mechanism of action of the peptide CIGB-552, which could be relevant in the design of future anticancer therapies.

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Therapeutic effects of gold nanoparticles synthesized using Musa paradisiaca peel extract against multiple antibiotic resistant Enterococcus faecalis biofilms and human lung cancer cells (A549)

Microbial Pathogenesis ◽

10.1016/j.micpath.2016.11.029 ◽

2017 ◽

Vol 102 ◽

pp. 173-183 ◽

Cited By ~ 47

Author(s):

S. Vijayakumar ◽

B. Vaseeharan ◽

B. Malaikozhundan ◽

N. Gopi ◽

P. Ekambaram ◽

...

Keyword(s):

Lung Cancer ◽

Gold Nanoparticles ◽

Enterococcus Faecalis ◽

Human Lung ◽

Human Lung Cancer ◽

Therapeutic Effects ◽

Antibiotic Resistant ◽

Musa Paradisiaca ◽

Human Lung Cancer Cells ◽

Peel Extract

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Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice

BMC Cancer ◽

10.1186/1471-2407-7-149 ◽

2007 ◽

Vol 7 (1) ◽

Cited By ~ 76

Author(s):

Xulong Zhang ◽

Jian Zhang ◽

Lihua Wang ◽

Haiming Wei ◽

Zhigang Tian

Keyword(s):

Lung Cancer ◽

Human Lung ◽

Human Lung Cancer ◽

Therapeutic Effects ◽

Xenograft Mice ◽

Stat3 Decoy

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Downregulation of Xeroderma Pigmentosum Complementation Group C Expression by 17-Allylamino-17-Demethoxygeldanamycin Enhances Bevacizumab-Induced Cytotoxicity in Human Lung Cancer Cells

Pharmacology ◽

10.1159/000509052 ◽

2020 ◽

pp. 1-15

Author(s):

Jyh-Cheng Chen ◽

Jen-Chung Ko ◽

Yong-Cing Taso ◽

Hsiang-Hung Cheng ◽

Tzu-Ying Chen ◽

...

Keyword(s):

Lung Cancer ◽

Growth Factor ◽

Xeroderma Pigmentosum ◽

Human Lung ◽

Excision Repair ◽

Lung Squamous Cell Carcinoma ◽

Complementation Group ◽

Human Lung Cancer ◽

Pcr Analysis ◽

Therapeutic Effects

Introduction: Xeroderma pigmentosum complementation group C (XPC) protein is an important DNA damage recognition factor involved in nucleotide excision repair and regulation of non-small-cell lung cancer (NSCLC) cell proliferation and viability. 17-Allylamino-17-demethoxygeldanamycin (17-AAG) blocks ATP binding to heat shock protein 90 (Hsp90), resulting in destabilization of Hsp90-client protein complexes. Vascular endothelial growth factor (VEGF) is a potent angiogenic growth factor expressed by many types of tumors. Bevacizumab (Avastin) is a humanized monoclonal antibody against human VEGF used as an antiangiogenesis agent in the therapy of many cancers, proving successful in increasing objective tumor response rate and prolonging overall survival in NSCLC patients. Methods: After the bevacizumab and/or 17-AAG treatment, the expressions of XPC mRNA were determined by quantitative real-time PCR analysis. Protein levels of XPC and phospho-AKT were determined by Western blot analysis. We used specific XPC small interfering RNA and PI3K inhibitor (LY294002) to examine the role of the AKT-XPC signal in regulating the chemosensitivity of bevacizumab and 17-AAG. Cell viability was assessed by the MTS assay and trypan blue exclusion assay. Results: In this study, bevacizumab decreased XPC expression in human lung squamous cell carcinoma H520 and H1703 cells via AKT inactivation. Enhancement of AKT activity by transfection with constitutively active AKT vectors increased XPC expression and cell survival after treatment with bevacizumab. In addition, 17-AAG synergistically enhanced bevacizumab-induced cytotoxicity and cell growth inhibition in H520 and H1703 cells, associated with downregulation of XPC expression and inactivation of AKT. Discussion/Conclusion: Together, these results may provide a rationale to combine bevacizumab with Hsp90 inhibitors in future to enhance therapeutic effects for lung cancer.

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