scholarly journals Hyperviscous Semen Causes Poor Sperm Quality and Male Infertility through Induction of Oxidative Stress

2019 ◽  
Vol 13 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Asghar Beigi Harchegani ◽  
Hamid Rahmani ◽  
Eisa Tahmasbpour ◽  
Alireza Shahriary
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Molecular Mechanisms ◽  
Semen Quality ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Cellular Mechanisms ◽  
Vitro Fertilization ◽  
Randomized Controlled Studies

Background/Aims: Semen hyperviscosity (SHV) is one of the significant factors involved in poor semen quality and male infertility. It also leads major problems during assisted reproduction techniques and in vitro fertilization process. Although influence of SHV on sperm quality, fertilization rate and male infertility have been widely considered, molecular and cellular mechanisms for these abnormalities are not well understood. In this review, we aimed to discuss the proposed cellular and molecular mechanisms of SHV on male reproductive system, the importance of oxidative stress (OS) and the mechanisms by which SHV induces OS and impairment of other antioxidants. Methods: A PubMed/Medline and EM-BASE search was performed using keywords: “hyperviscosity semen”, “oxidative stress”, and “male infertility”. Conclusion: OS induced by reactive oxygen species can be considered as a major mechanism in patients with hyperviscosity semen that is associated with DNA fragmentation, lipid peroxida-tion and sperm membrane disintegrity, apoptosis, depletion of antioxidants, and subsequently poor sperm quality and male infertility. Therefore, antioxidant therapy may improve main pathological effects of hyperviscosity semen, especially oxidative damages and inflammation, on sperm quality and function. Further, randomized controlled studies are necessary to confirm these results and make a comparison between effects of various antioxidants such as N-acethyl-cysteine and Curcumin on fertility problem in patients with hyperviscous semen.

scholarly journals Review on the role of antioxidant supplementation against oxidative stress: a human and animal approach to male fertility

2022 ◽  
Vol 11 (1) ◽  
pp. e43211125191
Author(s):  
Luana Nayara Gallego Adami ◽  
Valter Luiz Maciel Junior ◽  
João Diego Losano
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Male Fertility ◽  
Sperm Quality ◽  
Human Sperm ◽  
Original Data ◽  
Experimental Models ◽  
Antioxidant Supplementation ◽  
And Function

Male infertility is one important factor among the multifactorial causes of couple infertility, being oxidative stress one of the main related sources. Sperm is a specialized cell extremely susceptible to stress. To understand and mitigate this event, many studies have used different antioxidants, orally or in vitro supplementation, trying to improve sperm quality and function. Considering the extensive available literature regarding approaches and attempts to solve male fertility issues, the aim of this review is evaluating the effects of antioxidant supplementation on sperm, in both humans and experimental models with animals. This review selected original data from PubMed. The keywords used were: antioxidant, sperm, male fertility, antioxidant supplementation, male infertility; and the term "rodents" was added to the descriptors “antioxidant” and “male fertility”. Only studies published in indexed journals, in English, between 2015 and 2019 were included. This review involves i) human sperm and ii) rodent sperm. For the human approach, the search retrieved 496 articles and 80 were included, among which 28 studies were of in vitro antioxidant supplementation, 19 involved oral antioxidant supplementation and the remaining 33 concerned quantification of oxidants and antioxidants already present in the seminal samples. For the rodent approach, 152 articles were retrieved and 52 were included: 3 of varicocele, 11 of diabetes, 10 of therapeutic drugs, 3 of physical exercise, 10 of environmental exposure and 3 of heat stress. The remaining studies involved oxidative stress status in experimental models. Antioxidants use for reproductive purposes is increasing in an attempt to achieve better gametes and embryos. Vitamins C, B and E, selenium and zinc are the most commonly used antioxidants, with remarkable evidences in improving pathophysiological seminal conditions.

The role of melatonin in the reproductive realization of women with infertility

2017 ◽  
pp. 99-104
Author(s):  
V.V. Orlova ◽  
◽  
L.V. Suslikova ◽  
Keyword(s):  
Oxidative Stress ◽  
Reproductive Tract ◽  
Sperm Quality ◽  
Antioxidant Properties ◽  
Negative Influence ◽  
Level Of Evidence ◽  
Functional Layer ◽  
Active Forms Of Oxygen ◽  
Vitro Fertilization

The prevalence of infertility, including tubal-peritoneal genesis, led to the development of assisted reproductive technology (ART) as the most effective treatment and achievement of the expected pregnancy. But the success of ART is about 40% and depends on many factors: genetic, immunological, hormonal, age, control ovarial stimulation, sperm quality, quantity and quality received oocytes and embryos and implantation ability of endometrium. Therefore, it is important to consider the mechanisms and factors behind the successful implantation. Particular attention is given to the study of disturbances of oxidative stress and apoptosis in the reproductive tract of infertile women. Under the influence of adverse factors and in pathology, as a result of reducing the cell’s ability to neutralize free radicals and active forms of oxygen, antioxidant protection is disturbed, and oxidative stress develops, which promotes the onset of apoptosis. The programmed cell death occurs in the physiological functioning of the reproductive system: follicular atresia and regression of the functional layer of the endometrium when pregnancy is not occur. However, today the negative influence of oxidative stress and violation of the regulation of apoptosis on the reproductive function, namely the success of in vitro fertilization, has been confirmed. Also, the use of ART has some of the negative effects on gametes and embryos, which is accompanied by the emergence of oxidative stress and reduces the probability of pregnancy. Melatonin is a universal hormone that plays a key role in the functioning of sex hormones and has powerful antioxidant properties. As a key antioxidant regulator As a key antioxidant regulator at the biochemical and hormonal levels, melatonin positively influences the maturation of oocytes and the preparation of the endometrium before implantation. Therefore, there is a reasonable justification for the use of melatonin during treatment with ART. Many studies are devoted to the study of the effectiveness of the use of exogenous melatonin in the treatment of infertility, but to date, the level of evidence is insufficient for use in it ART. Key words: melatonin, infertility, oxidative stress, apoptosis, ART.

scholarly journals Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

2021 ◽  
Author(s):  
Iman Al-Saleh ◽  
Serdar Coskun ◽  
Reem Al-Rouqi ◽  
Tahreer Al-Rajudi ◽  
Chafica Eltabache ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
In Vitro Fertilization ◽  
Seminal Plasma ◽  
Follicular Fluid ◽  
Fertilization Rate ◽  
Reproductive Hormones ◽  
Vitro Fertilization ◽  
Poor Fertilization

This study examined the status of oxidative stress in 599 couples undertaking in vitro fertilization (IVF) treatment and its association with reproductive hormones, smoking, and outcomes. Oxidative stress biomarkers such as malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant capacity (TAC) were determined in follicular fluid and seminal plasma. Tail moment (TM) was used to evaluate DNA damage in sperm and granulosa cells. Reproductive hormones in serum and cotinine (COT) in urine, follicular fluid, and seminal plasma samples were determined. We used log-binomial multivariate regression to estimate relative risks for the association between oxidative stress/DNA damage and IVF binary outcomes (fertilization rate, biochemical pregnancy, clinical pregnancy, and live birth). We observed an increase in the oxidative stress markers MDA, 8-OHdG, and H2O2 in follicular fluid and seminal plasma, but a decrease in the antioxidant protection markers CAT and TAC. The MDA, 8-OHdG, and H2O2 levels were significantly higher in seminal plasma than in follicular fluid, while TAC, CAT, and TM were higher in follicular fluid (p < 0.001). Although women were nonsmokers, COT levels >50 µg/l were observed in 5.7% (urine) and 1.4% (follicular fluid). An increase in the CAT levels of follicular fluid was associated with a 48 and 41% decrease in the risk of poor fertilization rate (≤50%) and unsuccessful live birth, respectively. After the models were adjusted for hormonal factors, the associations remained the same, except that elevated TAC in follicular fluid became significantly associated with a decrease of 42% in the risk of poor fertilization rate (≤50%).

Comparison of the Outcome of Conventional in vitro Fertilization and Intracytoplasmic Sperm Injection in Moderate Male Infertility from Ejaculate

2013 ◽  
Vol 94 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Bao-Guo Xie ◽  
Yuan-Hua Huang ◽  
Wei-Jie Zhu ◽  
Song Jin
Keyword(s):  
In Vitro Fertilization ◽  
Male Infertility ◽  
Intracytoplasmic Sperm Injection ◽  
Semen Analysis ◽  
Implantation Rate ◽  
Fertilization Rate ◽  
Vitro Fertilization ◽  
Good Quality Embryo ◽  
Implantation And Pregnancy Rates

Objective: To evaluate whether couples with moderate male infertility should be treated with conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Patients and Methods: A total of 249 couples with moderate male infertility undergoing their first IVF/ICSI cycle were enrolled in the study. The couples were divided into two groups according to the results of semen analysis: moderate oligozoospermia (O group) and moderate oligoasthenozoospermia (OA group). Sibling oocytes were randomized into groups to be inseminated either by conventional IVF or ICSI. Fertilization rate, embryo quality, implantation rate, and clinical pregnancy rate were examined. Results: There was no difference in the fertilization, implantation, and pregnancy rates between conventional IVF and ICSI in either the O group or OA group (p > 0.05). Additionally, in the OA group, the good quality embryo rate was similar after IVF or ICSI (p > 0.05). However, in the O group, the good quality embryo rate was significantly higher after ICSI than after IVF (p < 0.05). Conclusions: Couples with moderate oligozoospermia or moderate oligoasthenozoospermia did not influence the major indices of IVF. Because of the uncertainties concerning the safety of ICSI, couples with moderate oligozoospermia or moderate oligoasthenozoospermia need not be subjected to this procedure.

scholarly journals Novel hydrogen‐producing Si‐based agent reduces oxidative stress, and improves sperm motility and in vitro fertilization rate in varicocoele

Andrology ◽  
2020 ◽  
Author(s):  
Yusuke Inagaki ◽  
Shinichiro Fukuhara ◽  
Ryoichi Imamura ◽  
Yuki Kobayashi ◽  
Sohei Kuribayashi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Sperm Motility ◽  
Fertilization Rate ◽  
Vitro Fertilization

Pathophysiological and age-specific mechanisms of morpho-functional changes in spermatozoa in infertility

2021 ◽  
Vol 20 (4) ◽  
pp. 17-26
Author(s):  
Anna A. Dotsenko ◽  
Alexander V. Polevshchikov
Keyword(s):  
Male Infertility ◽  
Russian Federation ◽  
Molecular Mechanisms ◽  
Married Couples ◽  
Vitro Fertilization ◽  
Pathophysiological Mechanisms ◽  
Functional Changes ◽  
Different Ages ◽  
The Russian Federation

The aim of the review is to analyze the causes and pathophysiological mechanisms of sperm alteration accompanying the development of male infertility, the sequence of the development of their dysfunctions in individuals of different ages. Male infertility is a worldwide problem; up to 20% of married couples are childless. In the Russian Federation, the problem is even more acute, which complicates the unfavorable demographic situation. Based on the generalization of the results of various screening studies, from 30 to 50% of men in the Russian Federation have impaired fertility. The review examines the main classifications and pathophysiological mechanisms of the development of male infertility. An extended assessment of changes in the morphology and functional properties of spermatozoa, relevant for the practice of in vitro fertilization, was carried out. The mechanisms of damage to spermatozoa and the sequence of development of degenerative changes in the cell are considered in detail. The mechanisms of sperm dysfunction development in men of different ages are compared. The conclusion is made about the need for further studies of the molecular mechanisms of fertility, deciphering the entire set of interactions between molecules and cells involved in the implementation of the reproduction function.

Mechanisms of diazinon effects on impaired spermatogenesis and male infertility

2018 ◽  
Vol 34 (9) ◽  
pp. 653-664 ◽  
Author(s):  
Asghar Beigi Harchegani ◽  
Alireza Rahmani ◽  
Eisa Tahmasbpour ◽  
Hamid Bakhiari Kabootaraki ◽  
Hossein Rostami ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Reproductive System ◽  
Molecular Mechanisms ◽  
Sperm Quality ◽  
Male Reproductive System ◽  
Testicular Atrophy ◽  
Sperm Dna Fragmentation ◽  
Organophosphate Insecticide ◽  
Cellular Mechanisms

Diazinon (DZN) is an organophosphate insecticide that has cytotoxic and pathological effects on the reproductive system. It causes a wide variety of pathological effects on the reproductive system such as testicular atrophy, disturbance in sex hormones, impaired spermatogenesis, low quality of sperm, and fertility problems. However, molecular and cellular mechanisms of its adverse effects are not well understood. General events such as testicular damage, inflammation, mitochondrial deficiency, DNA fragmentation, disintegration of sperm plasma membrane, apoptosis, and cell death are observed in DZN-exposed animals. Oxidative stress (OS) induced by reactive oxygen species may be a main mechanism, which can be associated with sperm DNA fragmentation, reduced integrity of sperm cell membrane, apoptosis, depletion of antioxidants, and subsequently poor sperm quality and male infertility. Therefore, identification of these pathways may provide valuable information regarding the mechanisms of DZN action on the male reproductive system. In this review, we aim to discuss the proposed cellular and molecular mechanisms of DZN action on male reproductive system, the importance of OS and mechanisms by which DZN induces OS and depletion of other antioxidants.

scholarly journals Clinical and Structural Features of Sperm Head Vacuoles in Men Included in the In Vitro Fertilization Programme

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Nina Fekonja ◽  
Jasna Štrus ◽  
Magda Tušek Žnidarič ◽  
Katja Knez ◽  
Eda Vrtacnik Bokal ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Human Sperm ◽  
Structural Features ◽  
Fertilization Rate ◽  
Sperm Head ◽  
Vitro Fertilization

The human sperm head vacuoles and their role in male infertility are still poorly understood. The aim of this study was to identify the clinical and ultrastructural features of human sperm head vacuoles in men included in the in vitro fertilization programme: men with normal (normozoospermia) and impaired sperm morphology (teratozoospermia). The sperm samples were observed under 6000-time magnification using motile sperm organelle morphology examination (MSOME). The proportion of sperm with head vacuoles was evaluated and related to the outcome of in vitro fertilization. The sperm of men with impaired sperm morphology was characterized by a higher proportion of sperm head vacuoles. The sperm head vacuoles were related to impaired semen quality (sperm concentration, motility, and morphology) but were not influenced by male factors (semen volume, height, age, weight, or body mass index). Moreover, sperm head vacuoles were related to impaired fertilization rate merely after classical in vitro fertilization (IVF), while there was no relation to pregnancy. In a subgroup of men, the sperm was fixed and observed by transmission electron microscopy (TEM). The ultrastructural study revealed that sperm head vacuoles are large nuclear indentations of various sizes and positions, packed with membranous material organized in membrane whorls (MW).

Sign in / Sign up

Export Citation Format

Share Document