Bifonazole Exerts Anti-Inflammatory Effects in Human Three-Dimensional Skin Equivalents after UVB or Histamine Challenge

2019 ◽  
Vol 32 (6) ◽  
pp. 337-343
Author(s):  
Laura Huth ◽  
Yvonne Marquardt ◽  
Ruth Heise ◽  
Katharina Fietkau ◽  
Norbert-Heinz Becker ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Skin Equivalents ◽  
Anti Inflammatory ◽  
Histamine Challenge

An Ultrasensitive Electrochemical Sensor for the Detection of Acetaminophen via a Three-Dimensional Hierarchical Nanoporous Gold Wire Electrode

The Analyst ◽  
2021 ◽  
Author(s):  
Lanting Qian ◽  
Reem Elmahdy ◽  
Antony Raj Thiruppathi ◽  
Aicheng Chen
Keyword(s):  
Electrochemical Sensor ◽  
Three Dimensional ◽  
Gold Wire ◽  
Nanoporous Gold ◽  
Wire Electrode ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Acetaminophen is one of the most commonly used non-steroidal anti-inflammatory drugs worldwide. However, due to the increasing popularity of this drug, overdosing and the contamination of ambient waterways have emerged...

scholarly journals Anti-proliferative and anti-inflammatory activities of the sea cucumber Holothuria polii aqueous extract

2018 ◽  
Vol 6 ◽  
pp. 205031211880954 ◽  
Author(s):  
Mike Kareh ◽  
Rana El Nahas ◽  
Lamis Al-Aaraj ◽  
Sara Al-Ghadban ◽  
Nataly Naser Al Deen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Matrix Metalloproteinase ◽  
Sea Cucumber ◽  
Three Dimensional ◽  
Ethanolic Extract ◽  
Two Dimensional ◽  
Aqueous Fraction ◽  
Anti Inflammatory ◽  
Metalloproteinase 9 ◽  
Holothuria Polii

Objective: Sea cucumbers are considered among the most important functional foods. Following bioassay guided fractionation, we assessed the anti-proliferative and anti-inflammatory activities of Holothuria polii ( H. polii) extracts. Methods: Sea cucumber ethanolic extract and the partially purified aqueous fractions were assessed for their anti-proliferative activities. These latter bioactivities were evaluated in the highly invasive MDA-MB-231 human breast cancer cells in two-dimensional and three-dimensional cultures using trypan blue exclusion assay. The tumor-suppressive effects of sea cucumber ethanolic extract and aqueous fractions were assayed by measuring the trans-well invasion of MDA-MB-231 cells and the expression of some epithelial mesenchymal transition markers using quantitative reverse-transcription polymerase chain reaction and western blot analysis. The anti-inflammatory activity of the aqueous fraction was tested by measuring the secreted levels of interleukin-6, nitric oxide, and matrix metalloproteinase 9 in endotoxin-induced mammary epithelial SCp2 cells and interleukin-1β in phorbol-12-myristate-13-acetate-activated human monocytic THP-1 cells. Results: Sea cucumber ethanolic extract and the aqueous fraction significantly decreased the proliferation of MDA-MB-231 cells by more than 50% at similar and noncytotoxic concentrations and caused an arrest in the S-phase of the cell cycle of treated cells. In contrast, petroleum ether, chloroform, ethyl acetate, and n-butanol organic fractions did not show any significant activity. Furthermore, sea cucumber ethanolic extract and aqueous fraction reduced the proliferation of MDA-MB-231 cells in three-dimensional cultures by more than 60% at noncytotoxic concentrations. In addition, treatment with these concentrations resulted in the loss of stellate outgrowths in favor of spherical aggregates and a 30% decrease in invasive properties. Both sea cucumber ethanolic extract and aqueous decreased the transcription of vimentin and the protein expression levels of vimentin and N-cadherin in three-dimensional cultures. The aqueous fraction decreased the levels of inflammatory markers interleukin-6, nitric oxide, and matrix metalloproteinase 9 in the mouse mammary SCp2 cells, and the level of interleukin-1β produced by phorbol-12-myristate-13-acetate-activated THP-1 human monocytic cells. Conclusion: The data reveal for the first time promising anti-proliferative and anti-inflammatory activities in H. polii water extract in two-dimensional and three-dimensional culture models.

Erratum to: Anti-inflammatory Effects of Atorvastatin in Human Glioblastoma Spheroids Cultured in a Three-dimensional Model: Possible Relevance to Glioblastoma Treatment

2017 ◽  
Vol 55 (3) ◽  
pp. 2111-2111
Author(s):  
Neda Bayat ◽  
Reza Izadpanah ◽  
Somayeh Ebrahimi-Barough ◽  
Abbas Norouzi-Javidan ◽  
Hooshang Saberi ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Human Glioblastoma ◽  
Anti Inflammatory

scholarly journals Design of a 3D BMP-2-Delivering Tannylated PCL Scaffold and Its Anti-Oxidant, Anti-Inflammatory, and Osteogenic Effects In Vitro

2018 ◽  
Vol 19 (11) ◽  
pp. 3602 ◽  
Author(s):  
Jae Lee ◽  
Hyunwoong Lim ◽  
Jae Ahn ◽  
Dongsik Jang ◽  
Seung Lee ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Calcium Deposition ◽  
Mrna Levels ◽  
Tnf Α ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Factor Α ◽  
Bone Morphogenic Protein 2 ◽  
Necrosis Factor

In this study, a novel three-dimensional (3D) bone morphogenic protein-2 (BMP-2)-delivering tannylated polycaprolactone (PCL) (BMP-2/tannic acid (TA)/PCL) scaffold with anti-oxidant, anti-inflammatory, and osteogenic activities was fabricated via simple surface coating with TA, followed by the immobilization of BMP-2 on the TA-coated PCL scaffold. The BMP-2/TA/PCL scaffold showed controlled and sustained BMP-2 release. It effectively scavenged reactive oxygen species (ROS) in cells, and increased the proliferation of MC3T3-E1 cells pre-treated with hydrogen peroxide (H2O2). Additionally, the BMP-2/TA/PCL scaffold significantly suppressed the mRNA levels of pro-inflammatory cytokines, including matrix metalloproteinases-3 (MMP-3), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), in lipopolysaccharide (LPS)-induced MC3T3-E1 cells. Furthermore, it showed outstanding enhancement of the osteogenic activity of MC3T3-E1 cells through increased alkaline phosphatase (ALP) activity and calcium deposition. Our findings demonstrated that the BMP-2/TA/PCL scaffold plays an important role in scavenging ROS, suppressing inflammatory response, and enhancing the osteogenic differentiation of cells.

scholarly journals Modulating the Chemical and Biological Properties of Cancer Stem Cell-Potent Copper(II)-Nonsteroidal Anti-Inflammatory Drug Complexes

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1677 ◽  
Author(s):  
Shin ◽  
Eskandari ◽  
Suntharalingam
Keyword(s):  
Three Dimensional ◽  
Biological Properties ◽  
Culture Studies ◽  
Apoptosis Pathway ◽  
Cytotoxicity Studies ◽  
Ic50 Value ◽  
Low Toxicity ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Micromolar Range

Copper(II) complexes bearing nonsteroidal anti-inflammatory drugs (NSAIDs) are known to potently kill cancer stem cells (CSCs), a subpopulation of tumour cells with high metastatic and relapse fidelity. One of the major disadvantages associated to these copper(II) complexes is their instability in the presence of strong cellular reductants (such as ascorbic acid). Here we present a biologically stable copper(II)-NSAID complex containing a bathocuproinedisulfonic acid disodium ligand and two indomethacin moieties, Cu(bathocuproinedisulfonic acid disodium)(indomethacin)2, 2. The copper(II) complex, 2 kills bulk breast cancer cells and breast CSC equally (in the sub-micromolar range) and displays very low toxicity against non-tumorigenic breast and kidney cells (IC50 value > 100 µM). Three-dimensional cell culture studies show that 2 can significantly reduce the number and size of breast CSC mammospheres formed (from single suspensions) to a similar level as salinomycin (an established anti-breast CSC agent). The copper(II) complex, 2 is taken up reasonably by breast CSCs and localises largely in the cytoplasm (>90%). Cytotoxicity studies in the presence of specific inhibitors suggest that 2 induces CSC death via a reactive oxygen species (ROS) and cyclooxygenase isoenzyme-2 (COX-2) dependent apoptosis pathway.

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