Neutrophil extracellular traps are increased in patients with acute ischemic stroke: prognostic significance

2017 ◽  
Vol 117 (10) ◽  
pp. 1919-1929 ◽  
Author(s):  
Juana Vallés ◽  
María Teresa Santos ◽  
Ana María Latorre ◽  
José Tembl ◽  
Juan Salom ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Proteolytic Enzymes ◽  
Demographic Data ◽  
Prognostic Significance ◽  
Neutrophil Extracellular Traps ◽  
Stroke Severity ◽  
Specific Marker ◽  
Glucose Levels ◽  
Extracellular Traps

SummaryNeutrophil extracellular traps (NETs) are networks of DNA, histones, and proteolytic enzymes produced by activated neutrophils through different mechanisms. NET formation is promoted by activated platelets and can in turn activate platelets, thus favoring thrombotic processes. NETs have been detected in venous and arterial thrombosis, but data in stroke are scarce. The aim of this study was to evaluate NETs in the plasma of patients with acute ischemic stroke and their potential association with baseline clinical characteristics, stroke severity, and one-year clinical outcomes. The study included 243 patients with acute ischemic stroke. Clinical and demographic data and scores of stroke severity (NIHSS and mRs) at onset and discharge were recorded. Markers of NETs (cell-free DNA, nucleosomes, and citrullinated histone 3 (citH3)), were determined in plasma. Patients were followed-up for 12 months after the ischemic event. NETs were significantly elevated in the plasma of patients with acute ischemic stroke when compared to healthy subjects. NETs were increased in patients who were over 65 years of age and in those with a history of atrial fibrillation (AF), cardioembolic stroke, high glucose levels, and severe stroke scores at admission and discharge. In multivariate analysis, elevated levels of citH3, the most specific marker of NETs, at onset were independently associated with AF and all-cause mortality at oneyear follow-up. NETs play a role in the pathophysiology of stroke and are associated with severity and mortality. In conclusion, citH3 may constitute a useful prognostic marker and therapeutic target in patients with acute stroke.

Abstract TMP26: Existence of Neutrophil Extracellular Traps in Arterial Thrombi from Patients with Acute Ischemic Stroke

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Wei Li ◽  
Huan Wang ◽  
Wuwei Feng ◽  
Meng Zhang
Keyword(s):  
Electron Microscopy ◽  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
White Blood Cells ◽  
Neutrophil Extracellular Traps ◽  
Immunofluorescence Staining ◽  
Large Artery ◽  
Extracellular Traps ◽  
Arterial Thrombus ◽  
Hematoxylin Eosin

Introduction: Neutrophil extracellular traps (NETs) are composed mainly of strands of filamentous DNA and nuclear proteins, such as chromatin or isoforms of histone and secretable neutrophil granular proteins. Recent studies have shown that NETs existed in the venous thrombi and might function as a scaffold and had implication for thrombolytic treatments. Hypothesis: NETs also exists in cerebral arterial thrombus collected from patients with acute ischemic stroke(AIS). Methods: Ten arterial thrombi were collected from 10 AIS patients (33-78 years old, 4 females) who were treated by endovascular therapy (eight of them accepted alteplase thrombolysis firstly)within 8 hours after symptoms onset. Hematoxylin-eosin (HE) staining, immunofluorescence staining (including anti-histone H3 and Hoechst 33342 which label DNA) and electron microscopy were performed to identify the existence of fibrin and/or NETs in thrombus. Results: There were 4 thrombi from middle cerebral artery, 3 from internal carotid artery and 3 from basilar artery. Cardioembolism were considered as etiology for 5 patients and large artery atherosclerosis was the etiology for another 5 patients. HE staining showed that large amounts of fibrin and white blood cells in all ten cerebral arterial thrombi. NETs structures were also identified in 10/10 cerebral arterial thrombi by immunofluorescence staining. Furthermore, the existence of neutrophil-released DNA network together with fibrin was confirmed using electron microscopy in all 10 thrombi. Conclusion: Our results directly demonstrated that NETs do exist in the arterial thrombi from patients with AIS. they may serve as another structural component in addition to fibrin in both cardiogenic and artery atherosclerotic thrombi. NETs can be a new potential target for the recanalization treatment. Keyword: Neutrophil extracellular traps (NETs); arterial thrombus; Acute ischemic stroke

scholarly journals Evaluating the Role of Oxidative Stress in Acute Ischemic Stroke

2020 ◽  
Vol 11 (01) ◽  
pp. 156-159
Author(s):  
Bindu Menon ◽  
Krishnan Ramalingam ◽  
Rajeev Kumar
Keyword(s):  
Oxidative Stress ◽  
Ischemic Stroke ◽  
Acute Stroke ◽  
Acute Ischemic Stroke ◽  
Lipid Membranes ◽  
Demographic Data ◽  
Stroke Severity ◽  
Stroke Patients ◽  
Antioxidant Power

Abstract Background The role of oxidative stress in neuronal injury due to ischemic stroke has been an interesting topic in stroke research. Malondialdehyde (MDA) has emerged as a sensitive oxidative stress biomarker owing to its ability to react with the lipid membranes. Total antioxidant power (TAP) is another biomarker to estimate the total oxidative stress in stroke patients. We aimed to determine the oxidative stress in acute stroke patients by measuring MDA and TAP. Materials and Methods MDA and TAP were determined in 100 patients with ischemic stroke and compared with that in 100 age- and sex-matched healthy adults. Demographic data, stroke severity measured by the National Institutes of Health Stroke Scale (NIHSS), and disability measured by the Barthel index (BI) were recorded. The association of MDA and TAP with other variables was analyzed by paired t-test. Results Of the whole sample, 74% represented males. The mean NIHSS score was 13.11 and BI was 38.87. MDA was significantly higher in stroke patients (7.11 ± 1.67) than in controls (1.64 ± 0.82; p = 0.00). TAP was significantly lower in stroke patients (5.72 ± 1.41) than in controls (8.53 ± 2.4; p = 0.00). The lipid profile and blood sugar levels were also significantly higher in stroke patients. There was no association of MDA and TAP with other variables. Conclusion We found that oxidative stress was associated with acute ischemic stroke. However, we could not establish an association between oxidative stress and the severity of acute stroke.

scholarly journals Thrombus Neutrophil Extracellular Traps Content Impair tPA-Induced Thrombolysis in Acute Ischemic Stroke

Stroke ◽  
2018 ◽  
Vol 49 (3) ◽  
pp. 754-757 ◽  
Author(s):  
Celina Ducroux ◽  
Lucas Di Meglio ◽  
Stephane Loyau ◽  
Sandrine Delbosc ◽  
William Boisseau ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Neutrophil Extracellular Traps ◽  
Extracellular Traps

The distribution indices of erythrocytes: which one for acute ischemic stroke?

2019 ◽  
Vol 45 (1) ◽  
pp. 65-75
Author(s):  
Nahide Ekici Günay ◽  
Nurullah Günay
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Linear Relationship ◽  
Survival Rates ◽  
Prognostic Significance ◽  
Fold Increase ◽  
Reference Intervals ◽  
Stroke Severity ◽  
Severity Scores ◽  
Poor Outcomes

Abstract Background The erythrocytes distribution indices are reported from same histogram but they have different reference intervals and calculated by distinct measurements. Objectives To explore whether the RDW-CV and RDW-SD values may predictive and prognostic significance in ischemic stroke patients and to clarify the relation each of these indices with stroke severity and 3-months survival. Materials and methods Cumulative survival rates were calculated with the Kaplan-Meier method, and life curves were compared to RDW-CV, RDW-SD quartiles. The risk factors were described with a multivariable cox proportional hazard model. Results In acute ischemic stroke, RDW-CV was more specific and RDW-SD was more sensitive (92% vs. 80%) (52% vs. 48%). RDW-CV values higher than 14.7% are associated with 11-fold increase in the risk of stroke; and the level of RDW-CV does show a linear relationship with the severity of stroke. However, when we evaluated RDW-SD, higher 43.55 fL RDW-SD values was have 16-fold increase in severity of stroke and have a relatively high linear relationship with stroke severity scores to RDW-CV. Conclusions RDW-CV and RDW-SD levels have different prognostic poor outcomes for interpreting in ischemic stroke events approach and these should be used alone and comparatively with the AIS predictive and prognostic approach.

scholarly journals Immunohistological Analysis of Neutrophils and Neutrophil Extracellular Traps in Human Thrombemboli Causing Acute Ischemic Stroke

2020 ◽  
Vol 21 (19) ◽  
pp. 7387
Author(s):  
Fabian Essig ◽  
Alexander M. Kollikowski ◽  
Mirko Pham ◽  
László Solymosi ◽  
Guido Stoll ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Plaque Rupture ◽  
Cerebral Arteries ◽  
Structural Complexity ◽  
Intravenous Thrombolysis ◽  
Neutrophil Extracellular Traps ◽  
Border Region ◽  
Imaging Data ◽  
Extracellular Traps

Ischemic stroke caused by thromboembolic occlusion of large cerebral arteries, such as the internal carotid (ICA) and/or the middle cerebral artery (MCA), is treated by mechanical thrombectomy (MT). MT allows salvage of the vessel-occluding thrombemboli, which most frequently originate from the left atrium or the left ventricle of the heart or from sites of plaque rupture within large arteries above the heart. Clot composition may influence the efficacy of (intravenous) thrombolysis and MT, respectively. We analyzed 37 human thrombemboli obtained from acute ischemic stroke patients during MT with special emphasis on histological staining of neutrophils and neutrophil extracellular traps (NETs). We found neutrophils as the main cellular component of cerebral thrombemboli but encountered considerable morphological heterogeneity. Neutrophils accumulated in the border region of fibrin-rich structures indicating possible interaction of neutrophils with distinct structural thrombembolus components. Web-like NETs were found in 35 of 37 thrombemboli in varying amounts. NETs were almost exclusively found within fibrin-rich areas. Importantly, stroke etiology, age and present oral anticoagulation was associated with morphological patterns and the amount of neutrophils. Correlation of histological data and imaging data revealed that relative Hounsfield units of cerebral thrombemboli positively correlated with the amount of red blood cells. In summary, our results demonstrate that neutrophils and NETs are substantial constituents of cerebral thrombemboli and contribute to their structural complexity.

scholarly journals Evaluation of serum ferritin as a prognostic marker in acute ischemic stroke: a prospective observational study

2020 ◽  
Vol 8 (12) ◽  
pp. 4282
Author(s):  
Ramesh Thanikachalam ◽  
Sathyan Elangovan ◽  
Appandraj Srivijayan
Keyword(s):  
Ischemic Stroke ◽  
Observational Study ◽  
Acute Ischemic Stroke ◽  
Prognostic Marker ◽  
Serum Ferritin ◽  
Prospective Observational Study ◽  
Tertiary Care ◽  
Prognostic Significance ◽  
Stroke Severity ◽  
The Mean

Background: Stroke is an important health problem causing of morbidity and mortality globally. Serum ferritin has gained clinical significance as a prognostic factor that can aggravate the cytotoxicity of brain ischemia. The present study investigated the prognostic significance of serum ferritin levels with the severity of stroke using NIHSS scale.Methods: It was a prospective observational study conducted on 60 patients with acute ischemic stroke admitted in the general medicine department of a tertiary care teaching hospital. Serum ferritin levels were assessed on all participants using the instrument “immulite”. National institute of health stroke scale scoring was applied at the time of admission and on the seventh day to assess the impairment caused by a stroke. IBM SPSS version 22 was used for statistical analysis.Results: The mean serum ferritin levels at admission in patients with severe stroke, moderate stroke and mild and less stroke were 337.41±58.76, 285.56±49.37, and 197.91±111.01 ng/mL, respectively. The mean serum ferritin levels at admission were 178.76±114.70 ng/mL and 341.91±62.292 ng/mL in subjects who did not deteriorate and those who deteriorated, respectively. Whereas the mean serum ferritin levels on the sixth day were 198.34±106.88 and 348.10±57.34 ng/mL in subjects who did not deteriorate and those who deteriorated, respectively.Conclusions: Serum ferritin has a significant positive correlation with the severity of acute ischemic stroke severity on admission (p<0.001) and negative correlation with the severity of acute ischemic stroke severity on seventh day of admission (p<0.001). Thus, serum ferritin can be used as a prognostic marker in acute ischemic stroke.

Correlation of Neutrophil to Lymphocyte Ratio with Expression of Neutrophil Extracellular Traps Within Stroke Emboli

2021 ◽  
pp. 159101992110655
Author(s):  
Jorge Arturo Larco ◽  
Mehdi Abbasi ◽  
Sarosh Irfan Madhani ◽  
Madalina Oana Mereuta ◽  
Yang Liu ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Blood Cells ◽  
White Blood Cells ◽  
Neutrophil Extracellular Traps ◽  
Vessel Occlusion ◽  
Average Percentage ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Extracellular Traps

Background & Purpose It has been hypothesized that circulating neutrophils have a direct correlation with the composition of emboli in acute ischemic stroke (AIS). The aim of this study is to evaluate the association between neutrophil-lymphocyte ratio (NLR) in peripheral blood and the expression of neutrophil extracellular traps (NETs) within stroke emboli. Methods Consecutive patients with acute ischemic stroke (AIS) due to large vessel occlusion (LVO) that underwent mechanical thrombectomy (MT) were included. Patients were divided into two groups based on NLR median value. Retrieved thrombi were histologically analyzed using Martius Scarlett Blue (MSB) for main thrombus components including red blood cells (RBCs), white blood cells (WBCs), fibrin and platelet. Immunohistochemistry staining for von Willebrand Factor (vWF) and anti-citrullinated H3 (H3Cit; NETs marker) was also performed. Results Samples from a total of 84 patients were included. The average percentage of RBCs, WBCs, fibrin, platelet, H3Cit, and vWF components in thrombi were 45.1%, 3.5%, 21.8%, 29.6%, 19.7% and 14.8% respectively. When stratifying by NLR group [low (≤3.94) versus high (>3.95)], high NLR group had significantly more WBCs (4.5%), fibrin (24.2%), H3Cit (22.7%) and vWF (17.1%) thrombus fractions compared to low NLR group. Additionally, RBC content (38.8%) was lower in the high NLR group. Conclusions NLR is correlated with the amounts of WBCs, fibrin, NETs and vWF within the thrombi retrieved from AIS patients due to LVO.

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