scholarly journals In vitro evaluation of the dissolving effect of carbonated beverages (Coca-Cola® ) and enzyme-based solutions on enteroliths obtained from horses: pilot study

2021 ◽  
Vol 58 ◽  
pp. e182579
Author(s):  
Sergio Andrés Vélez Gil ◽  
Juan José Patiño Marulanda ◽  
José Ramón Martínez Aranzales
Keyword(s):  
Pilot Study ◽  
Intestinal Lumen ◽  
Distilled Water ◽  
Water Control ◽  
Cellulase Enzymes ◽  
Santa Ana ◽  
Carbonated Beverages ◽  
Percentage Difference ◽  
Final Weight

Enteroliths are concretions of minerals that cause partial or total obstruction of the intestinal lumen, resulting in recurrent and chronic colic in horses. This pilot study aimed to evaluate the in vitro solvent effect of carbonated beverages (Coca-Cola® and Coca-Cola® Zero), and papain and cellulase enzymes (Robinson Pharma®, Santa Ana, CA, USA) on enteroliths obtained from horses. Six 51-grams samples of six enteroliths were assigned to six treatments of immersion solutions: T1, Coca-Cola®; T2: Coca-Cola® Zero; T3: distilled water + papain (90 mg) and cellulase (120 mg); T4: Coca-Cola® + papain and cellulase; T5: Coca-Cola® Zero + papain and cellulase; and, CT: distilled water (control). The volume for immersion in the assigned solution was 150 mL, at a pH of 7.1, using an incubation shaker (Heidolph® , Germany) at 37ºC and 25 rpm, for 72 h. The evaluation periods of the dissolution percentage (difference between the initial weight and final weight of the samples), were 0, 3, 12, 24, 36, 48, 60, and 72 h. After 72 h of immersion, solutions T4, T5, and T1 presented 47, 38.8, and 14.9% of dissolution, respectively. The other solutions did not have major differences with CT (control). Under the in vitro conditions of this pilot study, papain and cellulase enzymes potentiated the dissolving effect of the carbonated solutions on the enteroliths obtained from horses. Further studies are suggested since the existing literature is on the dissolution of phytobezoars and not of enteroliths. 

scholarly journals Analysis of the Influence of Food Colorings in Esthetic Orthodontic Elastomeric Ligatures

2016 ◽  
Vol 10 (1) ◽  
pp. 516-521 ◽  
Author(s):  
Vanessa Dias da Silva ◽  
Eduardo Martinelli S de Lima ◽  
Caroline Dias ◽  
Leandro Berni Osório
Keyword(s):  
Color Change ◽  
Black Tea ◽  
Control Group ◽  
Distilled Water ◽  
Water Control ◽  
Color Changes ◽  
Influence Of Food

Proposition: The purpose of this study was to evaluate in vitro the color changes of esthetic orthodontic elastomeric ligatures of different shades when exposed to four food colorings commonly found in the diet of patients. Materials and Methods: The sample consisted of esthetic orthodontic elastomeric ligatures in the colors pearl, pearl blue, pearl white and colorless, which were immersed for 72 hours in five different solutions: distilled water (control group), coffee, tea, Coca-Cola ® and wine. The color changes of the esthetic orthodontic elastomeric ligatures were measured with the aid of a spectrophotometer, at T1 - as provided by the manufacturer; and T2 - after colorings process. Results: The results indicated that the esthetic orthodontic elastomeric ligatures of all initial hues are susceptible to pigmentation. Among the evaluated colors, all changed the finished look and the color of the samples tested. In ascending order, the color of the samples was as follows: distilled water, Coca-Cola®, black tea, wine and coffee. Conclusion: The substances that have a greater potential for pigmentation in esthetic orthodontic elastomeric ligatures were black tea, wine and coffee, respectively. All shades of esthetic orthodontic elastomeric ligatures are susceptible to color change.

scholarly journals The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study

2017 ◽  
Vol 6 (1) ◽  
pp. 33-34
Author(s):  
Marta Radnai ◽  
Zsolt Rajnics
Keyword(s):  
Pilot Study ◽  
Hydrochloric Acid ◽  
Acid Solution ◽  
Oral Hygiene ◽  
Direct Contact ◽  
Photometric Method ◽  
Distilled Water ◽  
Dental Calculus ◽  
Carbamide Peroxide

ABSTRACT Introduction Calculus can accommodate teeth and prosthetic restorations when the patient's oral hygiene is poor. Hardened calculus cannot be removed by patients, it needs professional cleaning using ultrasonic scaler or hand instrument. Solutions dissolving and preventing accumulation of dental calculus may help to keep dentures clean. The aim of this investigation was to examine the effect of Periogen on dental calculus in vitro. Materials and methods Calculus was collected via scaling from patients during a routine dental checkup. The samples were stored in carbamide peroxide solution (5%) for 24 hours, then rinsed and stored in distilled water. First, the Ca2+ content of the calculus was determined by photometric method after treating with cc. hydrochloric acid solution for 1 hour. The calculus samples were put in Periogen solution, prepared according to manufacturer's instruction for 16 hours, then crushed and put in Periogen solution with the same concentrate for 4.5 hours. The Ca2+ dissolved from calculus was measured using same photometric method. Results Calculus samples contained 26 mg/100 mg Ca2+, which is similar to dentin (27–28 mg/100 mg) and to enamel (36 mg/100 mg). The Ca2+ dissolved from calculus after treating with cc. hydrochloric acid for 1 hour was considered 100%. Ca2+ dissolution was 1.5% after 16 hours (0.09%/h) and 5.45% (1.21%/h) for the next 4.5 hours after pulverizing the sample. This showed Periogen Ca2+ dissolution was 385 µg/100 mg after 16 hours direct contact with the material. Conclusion The experiment showed that pulverizing the previously hard calculus was done easily after soaking it in Periogen. The ability of Periogen to soften the calculus needs to be further investigated. How to cite this article Rajnics Z, Radnai M. The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study. Int J Experiment Dent Sci 2017;6(1):33-34.

scholarly journals In Vitro Evaluation of Physical and Mechanical Properties of Light-Curing Resin Cement: A Comparative Study

2020 ◽  
Vol 14 (01) ◽  
pp. 152-156
Author(s):  
Luís Felipe Espíndola-Castro ◽  
Oscar Felipe Fonseca de Brito ◽  
Larissa Gabrielle Assis Araújo ◽  
Izabella Luiza Aragão Santos ◽  
Gabriela Queiroz De Melo Monteiro
Keyword(s):  
Color Change ◽  
Surface Hardness ◽  
Color Stability ◽  
Resin Cement ◽  
The Other ◽  
Distilled Water ◽  
Water Control ◽  
Mean Values ◽  
Significance Level

Abstract Objective The aim of study was to evaluate in vitro the surface hardness, sorption, solubility, and color stability of three light-cured resin cements, namely RelyX Veneer (RLX), Variolink Veneer (VLK), and All Cem Veneer (ACV). Materials and Methods Cylindrical samples (15 × 1 mm) were made for each group using a metallic mold (n = 10). Vickers microhardness test was performed, and average hardness was calculated from three indentations (300 gf/15s) per sample. The sorption and solubility of the materials were evaluated according to ISO 4049:2009 based on three samples weighing: initial (m 1), after immersion in distilled water for 7 days (m 2), and final (after removal of all moisture [m 3]). The color change was observed using a digital spectrophotometer, at three different time points, baseline, 1 day, and 1 week of immersion in coffee and distilled water (control). Statistical Analysis Shapiro–Wilk test was used to analyze the normality of the data, and groups were compared using Kruskal–Wallis and Mann–Whitney tests. A significance level of 5% was used. Results RLX showed the highest microhardness mean values (36.96 VHN), but higher sorption (23.2 µg/mm3) and solubility (2.40 µg/mm3), with statistically significant differences with the other groups. For color stability, higher ∆E was observed for the samples immersed in coffee (p = 0.009). The VLK resin cement presented statistically significant differences from the other groups, with higher color changes in coffee at 1 day (15.14) and after 1 week (23.65). Conclusion RLX resin cement showed better hardness results. All materials tested performed satisfactorily for sorption and solubility according to ISO 4049:2009. All materials showed high-staining values after 1 week of immersion in coffee.

scholarly journals Can the chlorhexidine gluconate solution potentiate the staining of polycrystalline ceramic brackets?

2019 ◽  
Vol 18 ◽  
pp. e191647
Author(s):  
Catharina e Silva Monteiro Barros ◽  
Jose Guilherme Neves ◽  
Ana Paula Terossi de Godoi ◽  
Ana Rosa Costa ◽  
Mario Vedovello Filho ◽  
...  
Keyword(s):  
Red Wine ◽  
Color Change ◽  
Color Variation ◽  
Distilled Water ◽  
Water Control ◽  
Significance Level ◽  
Polycrystalline Ceramic ◽  
Ceramic Brackets ◽  
Ceramic Bracket

Aim: The present study aimed to assess, in vitro, the effect of chlorhexidine on the potentiation of polycrystalline ceramic bracket staining. Methods: Seventy-two polycrystalline ceramic brackets of upper right central incisors were divided into six groups (n=12) according to immersion solution. The groups were G1 - distilled water (control); G2 - chlorhexidine; G3 - coffee; G4 - red wine; G5 - chlorhexidine associated with coffee; and G6 - chlorhexidine associated with red wine. The samples were analyzed by means of a spectrophotometer according to the CIEL*a*b* system, and color change (ΔE*) was calculated. The readings were performed at the following times: T0 - After package removal and T1 - After staining. The data were analyzed by Kruskal Wallis and t tests (p<0.05) at 5% significance level. Results: The total color variation (ΔE*) was greater in the group that received chlorhexidine associated with red wine (p<0.05) and lower in the groups that received distilled water. All other groups showed greater value variations when compared to G1 and G2. Group G6 showed a greater color change due to the potentiation of chlorhexidine with the dye substance. Conclusion: It is concluded that chlorhexidine potentiates the staining caused by red wine in polycrystalline ceramic brackets.

scholarly journals Effect of Energy Drinks on the Inorganic Composition of a Composite Resin Material

2020 ◽  
pp. 1-5
Author(s):  
Danielson Guedes Pontes ◽  
Lara Pepita de Souza Oliveira ◽  
Diego Ferreira Regalado ◽  
Danielson Guedes Pontes ◽  
Cláudia Cândida Silva ◽  
...  
Keyword(s):  
Immersion Time ◽  
Composite Resin ◽  
In Vitro Study ◽  
Energy Drinks ◽  
Composite Resins ◽  
Distilled Water ◽  
Water Control ◽  
Inorganic Composition ◽  
The Individual

Composite resins are used in Dentistry to perform direct aesthetic restorations, and are a material whose properties can be influenced by the diet of the individual, especially in the face of the action of beverages with acid pH, such as energy drinks. The aim of this in vitro study was to evaluate the effect of two energy beverages on the chemical composition of a nanoparticulate composite resin, at two-time intervals. Filtek Z350XT (3M/ESPE) composite resin was used to produce thirty-six specimens (discs) of 6.0mm x 1.5mm dimensions. The specimens were initially immersed in distilled water for 24h at 37ºC and then randomly divided into two groups (n=18); according to the immersion time: 1 month (T1) and 3 months (T2); and in three subgroups (n=6) referring to immersion solutions: distilled water (control) and two energy beverages (RedBull and Monster). The inorganic composition of the specimens was measured by a spectrometry. The data were submitted to two-way ANOVA and Tukey tests. The main inorganic elements of this restorative material were respectively: zirconium (Zr), silicon (Si) and calcium (Ca), which reduced its percentage after immersion time. RedBull was the most active solution, promoting losses of the mineral Si after 1 month of immersion, of Zr, after 3 months, and of Ca after 1 month and after 3 months. Monster influenced losses only in Si, after 1 month. Distilled water did not induce significant losses. Thus, the two energy beverages reduced the inorganic percentage of the nanoparticulate composite resin evaluated in this research.

scholarly journals Effect of bleaching protocols on surface roughness and color change of high- and low-viscosity bulk-fill composite resins.

2020 ◽  
Vol 33 (2) ◽  
pp. 59-68
Author(s):  
Bruna Tavares ◽  
Fabiana França ◽  
Roberta Basting ◽  
Basting Turssi ◽  
Turssi Amaral
Keyword(s):  
Surface Roughness ◽  
Composite Resin ◽  
Color Change ◽  
In Vitro Study ◽  
High Viscosity ◽  
Composite Resins ◽  
Distilled Water ◽  
Water Control ◽  
Low Viscosity

The aim of this in vitro study was to evaluate the effect of bleaching protocols on the surface roughness (Ra), color change and surface micromorphology of a low-viscosity bulk-fill composite (Filtek Bulk Fill Flow, 3M ESPE), a highviscosity bulk-fill composite (Filtek Bulk Fill, 3M ESPE) and a conventional nanoparticulate composite resin (control) (Filtek Z350 XT, 3M ESPE). Forty samples of each composite (disks 5 mm in diameter and 2 mm thick) were randomly divided into four groups (n=10), according to bleaching protocol: a) 10% carbamide peroxide gel (Opalescence, Ultradent Products) (2 h/ day, for 14 days); b) 40% hydrogen peroxide gel (Opalescence Boost, Ultradent Products) (three bleaching sessions, once a week, 45 min/session); c) whitening rinse (Listerine Whitening Extreme, Johnson & Johnson) (2 min/day, for 14 days); and d) distilled water (control). The samples were submitted to triplicate readings (Ra and color [CIELAB parameters]) before and after contact with bleaching protocols. Micromorphology was analyzed in a scanning electron microscope (SEM). Ra and color parameters (ΔL, Δa, Δb and ΔE) were analyzed by generalized linear models (α=0.05). The Ra of the high-viscosity bulk-fill was significantly higher than that of the other composites (p<0.05). Ra increased significantly (p<0.05) and surface became more irregular (SEM analysis) in all the composite resins, regardless of the bleaching protocol (p<0.05). The high-viscosity bulk-fill composite resin group had significantly lower ΔE (p<0.05) than the nanoparticulate composite resin group immersed in distilled water. It was concluded that the characteristics of each resin significantly influenced the Ra more than the bleaching protocol. The high-viscosity bulk-fill resin presented minor color change.

scholarly journals Influence of arbuscular mycorrhizal fungi on the nematicidal properties of leaf extracts of Thymus vulgaris L.

2009 ◽  
Vol 46 (4) ◽  
pp. 230-240 ◽  
Author(s):  
N. Sasanelli ◽  
A. Anton ◽  
T. Takács ◽  
T. D’Addabbo ◽  
I. Birò ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Thymus Vulgaris ◽  
Distilled Water ◽  
Water Control ◽  
Leaf Extracts ◽  
Egg Hatch ◽  
Egg Masses ◽  
Meloidogyne Species

AbstractThe effect of arbuscolar mycorrhizal fungi (AMF) on the nematicidal activity of Thymus vulgaris against the rootknot nematodes Meloidogyne incognita and M. javanica was investigated in two in vitro experiments. In the first experiment egg masses of M. incognita and M. javanica were immersed for 3 weeks in aqueous leaf extracts of thyme plants non-inoculated or previously inoculated with Glomus mosseae or mixed AMF strains (Sclerocystis sinuosa, Glomus claroideum-1, G. claroideum-2 and G. claroideum-3). Thereafter the hatching test continued in distilled water for five more weeks. In the second experiment egg masses of both Meloidogyne species were exposed to the different thyme extracts for 4, 8 and 16 hours and then incubated in distilled water for 8 weeks. Distilled water and 5 mg/ml aqueous solution of fenamiphos nematicide were used as controls. Numbers of second stage juveniles emerging weekly were expressed as cumulative percentages of the total egg content of the egg masses. In the first experiment juvenile emergence from eggs of both Meloidogyne species immersed in thyme extracts for three weeks was completely suppressed since the first week. Hatching of eggs of M. incognita in all the extracts was significantly lower than that in water control, although emergence in the extract from uninoculated thyme plants was significantly higher than the others and no statistical different from that of aqueous fenamiphos solution. Emergence of M. javanica juveniles was significantly lower after immersion in all the extracts than in distilled water control and aqueous fenamiphos solution. In the second experiment a 4-hour exposure to the extract from thyme inoculated with G. mosseae and mixed AMF population significantly reduced the final hatch of M. incognita in comparison to distilled water. A 16-hour exposure to the extract from mixed AMF inoculated plants resulted in a significantly lower egg hatch than the shorter exposure times, whereas no statistical difference was found between 4 and 8 hour exposure to both extracts. Emergence of M. javanica juveniles was statistically lower than in water only after 16 hours exposure to the extracts from mixed AMF strains inoculated plants, but no difference was found among the different exposure times. Growth of T. vulgaris was significantly increased only by the infections of mixed AMF strains.

Effect of Whitening Toothpastes and Brushing on Microhardness of Esthetic Restorative Materials

2020 ◽  
Vol 44 (5) ◽  
pp. 296-301
Author(s):  
Fouad Salama ◽  
Faika Abdelmegid ◽  
Lamya Alhomaidhi ◽  
Sara Alswayyed ◽  
Shatha Alfarraj
Keyword(s):  
Surface Properties ◽  
Study Design ◽  
Distilled Water ◽  
Restorative Material ◽  
Water Control ◽  
Over The Counter ◽  
Significant Difference ◽  
Restorative Materials ◽  
And Control

OBJECTIVE: Whitening toothpastes are widely used. Hence, it is important to understand their effect on the surface properties of restorative materials. To evaluate the effect of three over-the-counter whitening toothpastes and toothbrushing simulation on microhardness of three restorative materials. Study design: Forty cylindrical (10×2mm) specimens were prepared from each restorative material and randomly assigned into four groups/10 each according to the whitening toothpastes used and distilled water (control). All specimens were measured for microhardness (Baseline–T1). The specimens were brushed with a soft brush using an in vitro tooth-brushing simulator with the assigned whitening toothpaste using the same setting for brushing cycles/load for all groups. Specimens were then measured for microhardness (T2) similar to baseline. Results: The highest (mean±SD) microhardness after application of the whitening toothpastes and brushing was recorded for Intense White and Filtek Z250 XT (127.6±1.8), followed by Optic White and Fuji ll LC (73.9±0.9) and Optic White and Photac Fill (72.7±1.3). There was statistically significant difference for microhardness between pre- and post-application of the whitening toothpastes and brushing for all tested restorative materials (P=0.0001). The microhardness of Filtek Z250XT with 3D White post-application of the whitening toothpastes and brushing was lowest compared to other toothpastes and control (P=0.0001). Conclusion: Microhardness increased after application of the whitening toothpastes and toothbrushing simulation for all combination of tested restorative materials and whitening toothpastes. The microhardness of FIiltek Z250XT with 3D White post-application of the whitening toothpastes and brushing was lowest compared to other toothpastes and control.

scholarly journals Effects on Dentin Treated with Eluted Multi-Mineral Varnish In Vitro

2012 ◽  
Vol 6 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Robert L Karlinsey ◽  
Allen C Mackey ◽  
Craig S Schwandt
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Control Group ◽  
Distilled Water ◽  
Water Control ◽  
Acid Ph ◽  
Ph Cycling ◽  
Demineralized Dentin ◽  
Acid Challenge

Purpose: The purpose of this pilot study was to assess the non-contact effects of a NaF varnish on the morphology and elemental compositions of sound and demineralized dentin in an in vitro pH cycling model. Methods: 3 mm diameter dentin cores were extracted from bovine teeth, mounted in acrylic rods, and ground and polished. Sound and demineralized (in 10 mL unstirred 50% citric acid (pH = 1.2) for two minutes) specimens were divided into four groups (N=6) corresponding to treatments with either water (control group) or non-contact Vanish® 5% NaF White Varnish with tri-calcium phosphate (3M ESPE). For the non-contact varnish group, one 0.50 ml unit-dose was applied to acrylic rods and positioned in the same stopper as three dentin specimens. The four groups were then cycled in a regimen consisting of three rounds of one-hour treatments with each round followed by immersion in a three-minute static acid challenge (0.3% citric acid, pH = 3.8). For each one-hour treatment immersion the test groups were immersed in distilled water. The specimens were thoroughly rinsed with distilled water after each treatment and after each acid challenge. After the last challenge, specimens were rinsed with distilled water and immersed in artificial saliva (AS) overnight. All treatments, acid challenges and immersion in AS solution were performed in an incubator set at 37°C. After three days, the specimens were assessed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Results: SEM and EDS results demonstrated the non-contact varnish group can provide mineralization benefits relative to the control group for both sound and demineralized dentin substrates. Depositions from mineral eluting from the varnish developed on the intertubular and intratubular regions alike, with the most prominent depositions appearing on initially demineralized dentin. Conclusions: In this observational study, acid-resistant mineral depositions were produced on the sound and dentin surfaces without direct application from a multi-mineral varnish containing fluoride, calcium and phosphate.

scholarly journals Spectrophotometric Analysis of Dental Enamel Staining to Antiseptic and Dietary Agents: In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Mukhatar Ahmed Javali ◽  
Mohasin Abdul Khader ◽  
Razan Mansour Alqahtani ◽  
Muna Jubran Almufarrij ◽  
Thamra Mohammed Alqahtani ◽  
...  
Keyword(s):  
Dental Enamel ◽  
In Vitro Study ◽  
Spectrophotometric Analysis ◽  
Distilled Water ◽  
Water Control ◽  
Calculus Formation ◽  
The Difference ◽  
Dietary Agents

Background/Objectives. Use of antiseptics as an adjunct to a traditional mechanical tooth brushing method has limited their application for long duration because of their side effects such as staining and calculus formation. The objective of this in vitro study is to analyse the staining effects of antiseptic mouthwashes on dental enamel and compare it with those containing nanoparticles, dietary agents, and distilled water (control). Material and Methods. 105 intact premolars extracted for orthodontic reasons and without any caries or anatomical defects were selected for analysis. The samples were randomly divided into 7 different groups of fifteen teeth each for different solutions. A spectrophotometer was used to assess the colorimeter analysis of buccal dental enamel surface at R1 (baseline examination), R2 (24 hours after immersion in different solutions), and R3 (after brushing). Statistical analysis was done using the Kolmogorov–Smirnov test and Levene’s test (p<0.05), respectively. One-way ANOVA was used to compare the difference in color (∆E) between the readings, R1, R2, and R3. Results. The mouthwash containing titanium dioxide (TiO2) nanoparticles produced the greater enamel discoloration compared to that of chlorhexidine. Brushing had little effect on removal of stains induced by all mouthwashes except for dietary solutions (lemon with sodium bicarbonate and olive with laurel) and distilled water (control). Conclusion. The results from this study show that mouthwashes containing TiO2 nanoparticles and other antiseptic mouthwashes cause change in color of the teeth and lead to poor esthetic appearance when compared to dietary and control solutions. Thus, future in vivo studies have to be conducted to confirm these findings as in vitro studies may not provide a reliable simulation of the clinical situations.

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