Comparative morphological and serological studies of three Anatolian Mountain frogs, Rana macrocnemis, R. camerani and R. holtzi (Anura, Ranidae)

2006 ◽  
Vol 27 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Mehmet Kutsay Atatür ◽  
Uğur Kaya ◽  
Hüseyin Arikan ◽  
İbrahim Ethem Çevik
Keyword(s):  
Blood Serum ◽  
Measurement Data ◽  
Disc Electrophoresis ◽  
Species Boundaries ◽  
Serum Samples ◽  
Frog Species ◽  
Serological Studies ◽  
Discriminant Analyses ◽  
Rana Macrocnemis

AbstractThree currently recognized species of Anatolian mountain frogs were analyzed for species boundaries. Meristic and proportional measurement data of three populations, including two topotypical ones, were evaluated using both univariate and discriminant analyses, and blood-serum samples were analyzed electrophoretically with polyacrylamide-disc electrophoresis. The results indicate that only two distinct mountain frog species inhabit Anatolia. We recommend that R. camerani from Mt Erciyes should be recognized as a synonym of R. macrocnemis.

scholarly journals PEDV Infection Generates Conformation-Specific Antibodies That Can Be Effectively Detected by a Cell-Based ELISA

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 303
Author(s):  
Wei-Ting Hsu ◽  
Chia-Yu Chang ◽  
Chih-Hsuan Tsai ◽  
Sung-Chan Wei ◽  
Huei-Ru Lo ◽  
...  
Keyword(s):  
Recombinant Baculovirus ◽  
Immunocytochemical Staining ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Perfect Agreement ◽  
Specific Antibodies ◽  
Diarrhea Virus ◽  
Serological Studies ◽  
A Cell ◽  
Infected Insect

Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes serious and highly contagious enteric disease in swine worldwide. In this study, we constructed a recombinant baculovirus (S-Bac) expressing full-length spike protein of the virulent epidemic genotype 2b (G2b) PEDV strain for serological studies of infected pigs. We found that most spike-specific antibodies produced upon PEDV infection in pigs are conformation-specific and they could be detected on S-Bac-infected insect cells by immunofluorescent assay, but they were insensitive to Western blot analysis, the typical method for antiserum analysis. These results indicated that spike conformation is crucial for serum recognition. Since it is difficult to purify trimeric spike membrane protein for conventional enzyme-linked immunosorbent assay (ELISA), we used S-Bac to generate a novel cell-based ELISA for convenient PEDV detection. We analyzed 100 pig serum samples, and our cell-based ELISA exhibited a sensitivity of 100%, a specificity of 97%, and almost perfect agreement [Cohen’s kappa coefficient value (κ) = 0.98] with immunocytochemical staining results. Our cell-based ELISA rapidly presented antigen for proper detection of conformation-specific antibodies, making PEDV detection more convenient, and it will be useful for detecting many viral diseases in the future.

Determination of urea in 10-?l blood serum samples with a urease reactor / ion-selective electrode cell

1983 ◽  
Vol 81 (1-2) ◽  
pp. 135-147 ◽  
Author(s):  
U. Thanei-Wyss ◽  
W. E. Morf ◽  
P. Lienemann ◽  
Z. Stefanac ◽  
I. Mostert ◽  
...  
Keyword(s):  
Blood Serum ◽  
Ion Selective Electrode ◽  
Selective Electrode ◽  
Serum Samples ◽  
Electrode Cell

scholarly journals TOXIC ELEMENTS CONTENT ANALYSIS OF BLOOD AND MILK OF COWS

2019 ◽  
pp. 92-97
Author(s):  
T. V Zaznobina ◽  
O. V Ivanova
Keyword(s):  
Blood Serum ◽  
Russian Federation ◽  
Toxic Elements ◽  
Blood Clot ◽  
Experimental Studies ◽  
Correlation Coefficients ◽  
Krasnoyarsk Krai ◽  
Economic Policies ◽  
Serum Samples ◽  
Safe Food

Currently, one of the main economic policies of the Russian Federation in the field of food security is production of safe food and consumer protection. The aim of the research was to study the content of such toxic elements as Zn, Cd and Pb in milk and blood of cows. Experimental studies were carried out at stud farms Plemzavod Tayozhny LLC in Suhobuzimsky District and OPH Solyanskoe LLC in Rybinsk District, Krasnoyarsk Krai, Russian Federation. The milksamples were studied by atomic absorption method; the blood serum samples, by mass spectrometry. Blood serum samples were obtained by sedimentation of whole blood and retraction of the blood clot followed by centrifugation. The obtained data were processed by the method of variation statistics with the calculation of statistical indicators. The lowest contents of Zn, Cd and Pb were found in blood serum of the cows of OPH Solyanskoe LLC; it was 0.038, 0.001, and 0.002 mg/l, respectively. The milk samplescollected at Plemzavod Tayozhny LLCcontained less heavy metals than those collected at OPH Solyanskoe LLC: Zn by 2.069 mg/l, Cd by 0.01 mg/l, and Pb by 0.061 mg/l (P>0.999). When calculating the correlation coefficients, weak positive and negative correlationswere established between Zn and Pb content in blood and milk. The detected amounts of toxic elements did not exceed the maximum permissible concentrations, except for Pb content in milk sampled at OPH Solyanskoe LLC, which was higher than the standard content by 0.026 mg/l.

scholarly journals Point-of-care high-sensitivity assay on PATHFAST as the backup in the emergency room

2021 ◽  
Vol 5 ◽  
pp. 239920262110550
Author(s):  
Joško Osredkar ◽  
Katja Krivic ◽  
Teja Fabjan ◽  
Kristina Kumer ◽  
Jure Tršan ◽  
...  
Keyword(s):  
Blood Serum ◽  
Troponin I ◽  
Point Of Care ◽  
High Sensitivity ◽  
Turnaround Time ◽  
Serum Samples ◽  
Linear Relationships ◽  
Accuracy And Precision ◽  
Laboratory Results ◽  
Key Indicators

Aim: Although the levels of cardiac troponin I (cTnI) have proved to be a useful diagnostic biomarker of acute myocardial infarction, there are a wide variety of point-of-care (POC) analysers, which provide measurements of cTnI. The aim of this study was to compare the results obtained by the ADVIA Centaur ultra-assay cTnI assay (us-cTnI), ADVIA Centaur high-sensitive cTnI assay (hs-cTnI) and a POC high-sensitivity assay using PATHFAST. We also aimed to explore total turnaround time (TAT) for laboratory results using the POC PATHFAST analyser. Methods: Samples from 161 patients were taken. Of these samples, 129 were tested with all three assays (us-cTnI, hs-cTnI and PATHFAST), and 32 samples were tested on PATHFAST for the comparison of whole blood, serum and plasma. Results: Comparison of the POC testing methods in this study demonstrated that there are strong linear relationships between all three cTnI assays (us-cTnI, hs-cTnI and POC on PATHFAST). Furthermore, we also show there are strong linear relationships between the two high-sensitive cTnI assays (hs-cTnI and PATHFAST) for blood serum samples, as determined by Passing–Bablok regression analyses. In our comparison of our new data with our older study, the TAT went down. Conclusion: The timeliness of laboratory results is, in addition to accuracy and precision, one of the key indicators of laboratory performance, and at the same time has a significant impact on the course of the patient’s condition. It is therefore important that the laboratory strives to meet the expectations of clinicians regarding the time from the order to the result of the analysis.

scholarly journals Role of O-polysaccharide antigen made from B. melitensis in the differential diagnosis of brucellosis in small ruminants

2021 ◽  
Author(s):  
P.K. Arakelyan ◽  
A.S. Dimova ◽  
A.V. Rudenko ◽  
N.V. Khristenko ◽  
V.T. Wolf ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Blood Serum ◽  
Small Ruminants ◽  
Natural Course ◽  
Primary Immunization ◽  
Serum Samples ◽  
Polysaccharide Antigen ◽  
Objective Indicator ◽  
Healthy Sheep

Out of 2942 blood serum samples from small ruminants of 10 flocks with a natural course of brucellosis caused by B. melitensis, 322 samples reacted with both antigens in the RID, of which 90 samples only with the O-PS M antigen (from B. melitensis), only with O-PS A-antigen (from B. abortus) reactive was not revealed. In healthy sheep immunized against brucellosis with the vaccine from strain 19 according to different schemes, only the O-PS M antigen was not found to react. Reaction with O-PS A- and M-antigens was observed in animals that were immunized twice subcutaneously at a dose of 40 billion mc. - after 2 months. after revaccination (60%), as well as in those reimmunized conjunctivally at a dose of 4 billion mc. according to the background of primary immunization subcutaneously at a dose of 40 billion mc. (10%) In animals immunized once or twice conjunctivally, reacting in RID with both antigens was not detected. Out of 2432 blood serum samples of small ruminants, 10 flocks with a brucellosis problem immunized against brucellosis with a vaccine from B.abortus strain 19 according to different schemes, 151 samples (6.2%) reacted positively with both O-PS antigens in RID with both O-PS antigens, of which only 86 samples (56.9%) reacted with O-PS M-antigen. The prevalence of indications of RID with O-PS M-antigen over RID with O-PS A-antigen (O-PS antigen made from Brucellae abortus) in small ruminants in one or another flock is characteristic of infection caused by brucellae melitensis at least in the absence, at least in the presence of the fact of immunization with a vaccine from the B. abortus 19 strain. RID with O-PS M-antigen is an objective indicator of epizootic danger and is able to differentiate brucellosis (B. melitensis) in small ruminants from vaccination-induced reactions (B. abortus 19).

Direct Fluorometric Determination of Total Cholesterol in Serum Using Derivatized Cholesterol Oxidase

2000 ◽  
Vol 54 (8) ◽  
pp. 1157-1162 ◽  
Author(s):  
Javier Galbán ◽  
José F. Sierra ◽  
José M. López Sebastian ◽  
Susana de Marcos ◽  
Juan R. Castillo
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Enzymatic Reaction ◽  
Direct Determination ◽  
Analytical Signal ◽  
Cholesterol Concentration ◽  
Serum Samples ◽  
Response Range

In this paper the use of cholesterol oxidase derivatized with a fluorescein derivative is proposed for the direct determination of total cholesterol in blood serum. The method is based on the changes in the fluorescence of the solution during the enzymatic reaction (λexe = 498 nm and λem 519 nm). A mathematical model which relates the analytical signal to the total cholesterol concentration was developed, and the model can also be used to obtain some of the thermodynamic constants of the process. The method has a linear response range up to 70 mg/L of cholesterol, a detection limit of 2.5 mg/L, and the precision was 1.0% (40 mg/L cholesterol, n = 10). The method was applied to total cholesterol determination in blood serum samples. The results were compared to those obtained by a commercial analyzer, and statistically similar results were obtained. The use of derivatized cholesterol oxidase makes it possible to simplify the methodology normally used in this type of determination (the indicator reaction is avoided and the number of reagents reduced), with the added advantage that the analytical signal is independent of the concentrations of O2 and cholesterol oxidase.

scholarly journals Adjuvanted Influenza Vaccines Elicits Higher Antibody Responses against the A(H3N2) Subtype than Non-Adjuvanted Vaccines

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 704
Author(s):  
Laura Sánchez de Prada ◽  
Iván Sanz Muñoz ◽  
Javier Castrodeza Sanz ◽  
Raúl Ortiz de Lejarazu Leonardo ◽  
José María Eiros Bouza
Keyword(s):  
Influenza Vaccine ◽  
Influenza A ◽  
Seasonal Influenza ◽  
The Elderly ◽  
Antibody Responses ◽  
Serological Response ◽  
Serum Samples ◽  
H3n2 Subtype ◽  
Serological Studies ◽  
Vaccination Campaigns

Background: vaccination is the best approach to prevent influenza infections so far. Serological studies on the effect of different vaccine types are important to address vaccination campaigns and protect our population. In our study, we compared the serological response against influenza A subtypes using the non-adjuvanted influenza vaccine (NAIV) in adults and the elderly and the adjuvanted influenza vaccine (AIV) in the elderly. Methods: We performed a retrospective analysis by hemagglutination inhibition assay (HI) of serum samples right before and 28 days after seasonal influenza vaccination during the 1996–2017 seasons. Conclusions: The AIV presents better performance against the A(H3N2) subtype in the elderly whereas the NAIV induces a better response against A(H1N1)pdm09 in the same group.

scholarly journals Modification of blood serum DSC profiles by sauna treatments in cross-country skiers during the exercise cycle

2020 ◽  
Vol 142 (5) ◽  
pp. 1927-1932
Author(s):  
Klaudia Duch ◽  
Anna Michnik ◽  
Ilona Pokora ◽  
Ewa Sadowska-Krępa
Keyword(s):  
Blood Serum ◽  
Recovery Process ◽  
Individual Variability ◽  
Exercise Session ◽  
Large Individual ◽  
Serum Samples ◽  
Scanning Calorimetry ◽  
Post Exercise ◽  
Higher Temperature ◽  
Cross Country

AbstractThe study has evaluated the effect of sauna treatments on the blood serum of professional cross-country skiers (3 women and 6 men) during two exercise sessions: the first without any treatments and the second after ten sauna treatments. Aqueous solutions of serum samples from the blood of athletes collected in four stages in both exercise sessions: at rest, after exercise, after 1 h and 24 h rest and additionally only at rest after the first sauna treatment, were measured by means of differential scanning calorimetry (DSC). DSC results allowed to register characteristic post-exercise changes in the profile of thermal denaturation transition of serum. A trend of these changes was similar regardless of the use of sauna, but their intensity was stronger in the exercise session after the sauna treatments. In particular, the differences relating to the use of sauna have revealed in the higher temperature range of serum denaturation transition. Regarding the recovery process after exercises, a large individual variability was observed in both sessions.

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