Description of Discocriconemella sinensis n. sp. (Nematoda: Criconematidae) from the rhizosphere of Camellia sinensis in China

Nematology ◽  
2019 ◽  
Vol 21 (8) ◽  
pp. 779-792 ◽  
Author(s):  
Munawar Maria ◽  
Ruihang Cai ◽  
Sergei A. Subbotin ◽  
Jingwu Zheng
Keyword(s):  
New Species ◽  
Close Relationships ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
28S Rrna ◽  
Pharyngeal Bulb ◽  
En Face ◽  
A New Species ◽  
Partial 18S ◽  
Group 1

Summary Discocriconemella sinensis n. sp. isolated in Hangzhou, Zhejiang, China, from the rhizosphere of Camellia sinenis is described. The new species was characterised morphologically and molecularly. Important morphological details were elucidated by SEM photographs. The new species is characterised by an uninterrupted rounded cephalic disc, en face showing a rectangular labial plate with slit-like oral apertures, labial plate surrounded by slightly elevated projections resembling rudimentary lobes, R = 66 (64-69), Rex = 21 (17-24), stylet 74 (67-81) μm long, excretory pore located at the base of the pharyngeal bulb, vulva open, tail short and conoid with a lobed terminus. The species belongs to the group 1 lip pattern. Morphologically, it is most similar to D. discolabia, D. mauritiensis, D. mineira and D. perseae. This is the first new Discocriconemella species described from China. Phylogenetic analyses based on analysis of the D2-D3 expansion segments of the 28S rRNA, ITS rRNA, partial 18S rRNA, and COI gene revealed that the new species formed a separate clade from other criconematid species, thereby supporting its status as a new species of the genus. The new species showed close relationships with Criconemoides informis.

Morphological and molecular characterisation of Helicotylenchus ciceri n. sp. and H. scoticus Boag & Jairajpuri, 1985 (Nematoda: Hoplolaimidae) from Iran

Nematology ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 611-626
Author(s):  
Fariba Mohammadi Zameleh ◽  
Akbar Karegar ◽  
Reza Ghaderi ◽  
Abbas Mokaram Hesar
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
Rrna Genes ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Closely Related Species ◽  
Partial 18S ◽  
Molecular Characters

Summary Helicotylenchus ciceri n. sp. and H. scoticus are described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by a conical and truncated lip region with five or six distinct annuli, stylet 32-37 μm long with anteriorly concave knobs, secretory-excretory pore posterior to the pharyngo-intestinal valve, dorsally convex-conoid tail with a terminal projection, phasmids 14 (7-20) annuli anterior to the level of anus, empty spermatheca and absence of males. Intraspecific variation of 16 populations of H. scoticus, collected from chickpea and lentil fields in Kermanshah province, western Iran, is discussed. The results of the phylogenetic analyses based on the sequences of the partial 18S rRNA, D2-D3 expansion segments of 28S rRNA and ITS rRNA genes are provided for the studied species, confirming their differences from each other and determining the position of them and their relationships with closely related species.

Morphological and molecular characterization of Hemicycliophora subbotini n. sp. (Tylenchida: Hemicycliophoridae) from China

Zootaxa ◽  
2018 ◽  
Vol 4433 (1) ◽  
pp. 161 ◽  
Author(s):  
MUNAWAR MARIA ◽  
RUIHANG CAI ◽  
NAN QU ◽  
PABLO CASTILLO ◽  
JINGWU ZHENG
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Oral Disc ◽  
Pharyngeal Bulb ◽  
En Face ◽  
The Status ◽  
A New Species ◽  
Morphological And Molecular Characterization ◽  
Lateral Plates

Sheath nematodes of the genus Hemicycliophora are migratory root-ectoparasites of many crops, but the damage is documented for only a few species. Hemicycliophora subbotini n. sp., isolated in Hangzhou, Zhejiang, China, from the rhizosphere of camphor tree (Cinnamomum camphora (L.) Presl), is described and characterized morphologically and molecularly with important morphological details elucidated by SEM studies. The new species can be characterized by the conoid lip region, with bulging oral disc protruding beyond the first annulus in some specimens. Oral disc narrow ovate, amphidial apertures covered by lateral plates, lying in the same plane as oral disc when observed en face. Stylet 118 (108–125) μm long and flexible, stylet knobs concave shaped having a slight cavity. The excretory pore is located 1–2 annuli posterior to the base of pharyngeal bulb. Vulval lips slightly modified with small vulval sleeve, spermatheca rounded to oblong. Anus located 18 (15–22) annuli posterior to vulva. Tail elongated, with conoid or slightly offset spike having a narrowly rounded terminus. The study provides the first description of a new Hemicycliophora species from China. Phylogenetic analyses based on D2–D3 expansion segments of the 28S and ITS fragments revealed the new species forming a separate clade from other Hemicycliophora species which supported the status as a new species of this genus. 

Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Morphological Characters ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

Morphometric and molecular data of the two digenean species Lasiotocus lizae Liu, 2002 (Monorchiidae) and Paucivitellosus vietnamensis sp. n. (Bivesiculidae) from mullet fish in Tonkin Bay, Vietnam

2016 ◽  
Vol 91 (3) ◽  
pp. 346-355 ◽  
Author(s):  
D.M. Atopkin ◽  
V.V. Besprozvannykh ◽  
H.D. Ngo ◽  
N. Van Ha ◽  
N. Van Tang ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Morphological Features ◽  
Digenean Species ◽  
Molecular Differentiation ◽  
A New Species

AbstractAdults of Lasiotocus lizae Liu, 2002 (Monorchiidae) were found in the mullet Liza longimanus (Günther) from Tonkin Bay, near Cat Ba Island, Vietnam. In this region, flukes belonging to the genus Paucivitellosus (Bivesiculudae) were found in Liza subviridis (Valenciennes), Liza engeli (Bleeker) and Valamugil seheli (Forskåi). Results of investigations showed that morphological features of L. lizae (like L. glebulentus Overstreet, 1971) do not meet the criteria of the genus Lasiotocus. In addition, L. lizae is highly differentiated from other species of Lasiotocus from which molecular data were obtained, including L. arrhichostoma Searle, Cutmore et Cribb, 2014 and L. typicum (Nicoll, 1912). Phylogenetic analyses revealed that L. lizae differs considerably from other species of the genus Lasiotocus presented in the GenBank database. We have identified a new species of the genus Paucivitellosus–P. vietnamensis sp. n. – from L. subviridis, which differs from P. fragilis Coil, Reid et Kuntz, 1965 by metrical and molecular (18S rRNA) data, and from P. hanumanthai Mani, 1989 by metric features. Our results also show considerable molecular differentiation between P. vietnamensis sp. n. and Paucivitellosus spp. recovered from L. engeli and V. seheli in Vietnam.

scholarly journals Foxed intra- and interspecific differentiation in Leptogorgia (Octocorallia: Gorgoniidae). A description of a new species based on multiple sources of evidence

2017 ◽  
Vol 81 (2) ◽  
pp. 147 ◽  
Author(s):  
M. Mar Soler-Hurtado ◽  
César Megina ◽  
Annie Machordom ◽  
Pablo J. López-González
Keyword(s):  
New Species ◽  
Incomplete Lineage Sorting ◽  
Marine Invertebrate ◽  
Phylogenetic Analyses ◽  
28S Rrna ◽  
Ancestral Polymorphism ◽  
Morphometric Characters ◽  
Pattern Size ◽  
Correlated Characters ◽  
A New Species

The challenges of delimiting and identifying marine invertebrate species impede estimations of true biodiversity. This is particularly true in the case of gorgonian diversity, in which only classical morphological characters (e.g. branching pattern, size and colouration of the colony and sclerites, etc.), which can be homoplastic and continuous, have been used. In this study, using an integrative taxonomic approach, we analysed two morphs initially considered as two eco-typical variants of Leptogorgia alba Duchassaing and Michelotti, 1864, living sympatrically in the littoral area of Ecuador. We explored the use of classical morphological and morphometric characters to delimit species in combination with the analyses of molecular markers (mtMutS, CoxII-Igr-CoxI, ITSs, and 28S rRNA) to infer phylogenetic relationships. Based on our results, two species should be considered, L. alba and a new species, L. manabiensis n. sp., which showed distinguishing morphological features that cannot be attributed to phenotypic plasticity. Both species also showed significant differences in morphometric, non-correlated characters in all size classes. The phylogenetic analyses showed a polyphyletic L. alba - L. manabiensis n. sp. species complex, and ancestral polymorphism and incomplete lineage sorting as possible evolutionary processes leading to this pattern. In conclusion, the combination of morphological and morphometric evidences provides the best support for the identification and delimitation of these challenging species. In addition, molecular analyses, mainly supported by nuclear markers, allow fundamental aspects of the evolutionary history of these organisms to be discerned.

A new cystoid nematode, Cryphodera japonicum n. sp. (Nematoda: Heteroderidae), from the rhizosphere of Japanese Podocarpus macrophyllus

Nematology ◽  
2020 ◽  
Vol 22 (8) ◽  
pp. 891-905
Author(s):  
Jianfeng Gu ◽  
Munawar Maria ◽  
Yiwu Fang ◽  
Honghong Wang ◽  
Kan Zhuo
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Coi Gene ◽  
Second Stage ◽  
Close Relationship ◽  
Podocarpus Macrophyllus ◽  
Stylet Length ◽  
A New Species ◽  
Partial 18S

Summary Cryphodera japonicum n. sp., detected at Ningbo port, China, from the rhizosphere of imported Japanese Podocarpus macrophyllus, is described. The new species is characterised by females with a globose body, protruding vulval lips, slightly concave anus-vulva profile, a stylet length of 37.2 (31.1-41.3) μm and a vulva-anus distance of 38.1 (34.7-44.1) μm. Males possess two lip annuli, a stylet length of 27 (22.5-30.6) μm long, three lines in lateral fields and a spicule 21.5 (19.1-23.0) μm long. Second-stage juveniles have bodies 543 (506-588) μm long, three lip annuli, a stylet 31.7 (29.2-34.5) μm long, three lines in the lateral fields, a conoid tail with a narrow rounded terminus, and a relatively long hyaline region occupying half of the tail length. Phylogenetic analyses based on the D2-D3 expansion segments of the 28S, ITS, partial 18S rRNA, and COI gene revealed the unique position of this species with other heteroderid nematodes, supporting its status as a new species of Cryphodera. The new species showed a close relationship with C. brinkmani.

Paratylenchus jasmineae sp. n. (Nematoda: Paratylenchinae) from rhizosphere of Jasminum sambac in India

Nematology ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 469-478 ◽  
Author(s):  
Victor Phani ◽  
Vishal Singh Somvanshi ◽  
Uma Rao ◽  
Matiyar Rahaman Khan
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
28S Rrna ◽  
Lateral Field ◽  
Lateral Lines ◽  
Stylet Length ◽  
Jasminum Sambac ◽  
A New Species

Summary A new species of pin nematode, Paratylenchus jasmineae sp. n., was isolated and described from the rhizosphere of jasmine in India. The new species can be diagnosed by having a relatively larger body length of 248-355 μm, stylet length of 24.6-28.5 μm, lateral field with four lateral lines, the presence of small post-vulval uterine sac and absence of advulval flaps in the females. Additional morphological characters included continuous lip region with small projected submedian lobes, large and oblong-shaped spermatheca, the presence of prevulval swelling, and arcuate conoid tail with finely rounded terminus. Molecular characterisation of the species was carried out based on internal transcribed spacer sequence (ITS rRNA) and D2-D3 expansion segment of 28S rRNA subunit. Phylogenetic analyses based on ITS and D2-D3 molecular markers resulted in clear separation of P. jasmineae sp. n. from other Paratylenchus species.

scholarly journals A troglobitic species of the centipede Cryptops (Chilopoda, Scolopendromorpha) from northwestern Botswana

ZooKeys ◽  
2020 ◽  
Vol 977 ◽  
pp. 25-40 ◽  
Author(s):  
Gregory D. Edgecombe ◽  
Nesrine Akkari ◽  
Edward C. Netherlands ◽  
Gerhard Du Preez
Keyword(s):  
South Africa ◽  
New Species ◽  
New Zealand ◽  
16S Rrna ◽  
Molecular Phylogenetics ◽  
18S Rrna ◽  
Temperate Species ◽  
28S Rrna ◽  
Oxidase Subunit ◽  
A New Species

A new species of Cryptops, C. (Cryptops) legagussp. nov., occurs in caves in the Koanaka and Gcwihaba Hills in northwestern Botswana. Bayesian molecular phylogenetics using 18S rRNA, 28S rRNA, 16S rRNA and cytochrome c oxidase subunit I corroborates a morphological assignment to the subgenus Cryptops and closest affinities to southern temperate species in South Africa, Australia and New Zealand. The new species is not conspicuously modified as a troglomorph.

Aphelenchoides salixae n. sp. (Nematoda: Aphelenchoididae) isolated from Salix alba in western Iran

Nematology ◽  
2017 ◽  
Vol 19 (6) ◽  
pp. 697-707 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Mohammad Tahmoures ◽  
Weimin Ye
Keyword(s):  
New Species ◽  
Rrna Genes ◽  
28S Rrna ◽  
Salix Alba ◽  
Western Iran ◽  
Female Tail ◽  
Lateral Lines ◽  
Conical Bearing ◽  
A New Species ◽  
Group 1

Aphelenchoides salixaen. sp. was isolated from wood and bark samples of a dead willow in Kermanshah Province, western Iran. The nematodes were collected directly from wood samples and were successfully reared onBotrytis cinerea. The new species has a body length of 567 (507-620) μm (females) and 382 or 400 μm (males). The cuticle is weakly annulated with four lateral lines. Lip region offset. The stylet is 9.5 (9-10) μm long with small basal swellings. The excretory pore is locatedcaone body diam. posterior to the metacorpus valve. The spicules are short with the dorsal limb 14 or 15 μm long (n = 2), apex and rostrum rounded and only slight offset. The female tail is cylindrical with a broadly rounded terminus. The male tail is conical, bearing six (2 + 2 + 2) caudal papillae with a hamate mucron at terminus. The new species belongs to the Group 1 category ofAphelenchoidesspecies and is close to seven known species, namely:A. capsuloplanus,A. confusus,A. limberi,A. obtusicaudatus,A. obtusus,A. orientalisandA. rotundicaudatus. Molecular analyses of the partial 18S, D2-D3 expansion segments of the 28S rRNA genes and internal transcribed spacer (ITS) supported it as a new species and that the new species is closest toA.besseyi,A. fujianensisandA. ritzemabosiin dendrograms inferred using 18S and 28S D2-D3 genes.

An integrative description of a new tardigrade species Mesobiotus romani sp. nov. (Macrobiotidae: harmsworthi group) from the Ecuadorian Pacific coast

Zootaxa ◽  
2018 ◽  
Vol 4450 (5) ◽  
pp. 550 ◽  
Author(s):  
MILENA ROSZKOWSKA ◽  
DANIEL STEC ◽  
MAGDALENA GAWLAK ◽  
ŁUKASZ KACZMAREK
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Pacific Coast ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Mitochondrial Coi ◽  
Lichen Sample ◽  
Taxonomic Description ◽  
North Western ◽  
A New Species

In a mixed moss and lichen sample collected in Esmeraldas Province in north-western Ecuador, 20 tardigrades and 11 eggs, belonging to a new species of the genus Mesobiotus, were found. In addition to the traditional taxonomic description with morphometrics, light and scanning microscopy imaging, we also provide nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragments of the new species. Based on the egg chorion morphology, Mesobiotus romani sp. nov. is the most similar to: M. binieki, M. coronatus, M. dimentmani, M. patiens, M. perfidus, M. philippinicusi, M. pseudoblocki, M. pseudocoronatus, M. pseudopatiens, M. radiatus, M. rigidus, M. simulans and M. wuzhishanensis, but differs mainly by some specific characters of both egg and adult morphology, and morphometrics. 

Sign in / Sign up

Export Citation Format

Share Document