Supplementary characterisation of Bursaphelenchus leoni Baujard, 1980 (Tylenchina: Aphelenchoididae) found in Pinus massoniana from Zhejiang Province, China, with proposal of B. borealis Korentchenko, 1980 as a junior synonym

Nematology ◽  
2020 ◽  
Vol 22 (9) ◽  
pp. 1061-1078
Author(s):  
Hongmei Li ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Kai Guo ◽  
Ute Schönfeld ◽  
...  
Keyword(s):  
Excretory Pore ◽  
Chinese Population ◽  
Posterior Margin ◽  
Pinus Massoniana ◽  
Molecular Data ◽  
Nerve Ring ◽  
Zhejiang Province ◽  
Junior Synonym ◽  
Lateral Field ◽  
Female Tail

Summary Bursaphelenchus leoni is redescribed, using morphology, morphometrics and molecular data, based on a population isolated from dead Pinus massoniana in Zhejiang Province, China. It is characterised by a lateral field with three lines, a 12-15 μm long stylet with small basal swellings, excretory pore located one body diam. posterior to nerve ring, spicules mitten-shaped, dorsally and ventrally arcuate, with lamina smoothly tapering towards broadly rounded distal tip without hook-like process in the Chinese population, condylus broadly rounded with a small dorsally bent hook, rostrum triangle or conical with finely rounded tip. The bursal flap is long, spade-like with posterior margin truncate or irregular. A 2-3 μm long vulval flap is present. The female tail is long and conical (c′ = 5.3-6.9), with the terminus finely rounded, or occasionally pointed. The Zhejiang population of B. leoni is close to B. eidmanni and B. silvestris, which all belong to the leoni-group sensu Braasch et al. (2009). Comparison with the description of B. borealis led to the conclusion that B. leoni and B. borealis are conspecific, the latter being regarded as a junior synonym of the former.

Redescription of Ektaphelenchoides compsi Baujard, 1984 (Tylenchina: Aphelenchoididae) isolated from Pinus massoniana in Fujian Province, China

Nematology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Hongmei Li ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Maria Munawar
Keyword(s):  
Phylogenetic Analyses ◽  
Posterior Part ◽  
Pinus Massoniana ◽  
Distal Region ◽  
Nerve Ring ◽  
Rrna Genes ◽  
Female Gonad ◽  
Fujian Province ◽  
Lateral Field ◽  
Female Tail

Summary Ektaphelenchoides compsi is redescribed morphologically with new molecular characterisation. It was isolated from a dead Pinus massoniana tree in Ningde City, Fujian Province, China. Detailed morphology of the spicule, female gonad, hemizonid position, arrangement of male caudal papillae and female tail terminus shape are documented. It is characterised by a lateral field with three lines (forming two bands), tripartite stylet 17.8 (17.0-19.4) μm long without basal thickenings, metacorpus rectangular with anterior 40% granular and posterior part weakly muscular, metacorpal valve slightly posterior to middle of metacorpus, excretory pore at level of nerve ring, vagina with thickened walls and strongly developed muscular bundles, vulval lips slightly protuberant, vulval flap absent, distal region of post-vulval uterine sac appearing as a weakly developed oogonia, anus and rectum indistinct, female posterior part (‘tail’) dorsally convex, conical, terminal region contracted into a bluntly pointed tip. The spicules are arcuate, 15.6 (14.3-16.3) μm along the chord, lamina smoothly curved to distal end, capitulum slightly concave, condylus well-developed with broadly rounded tip and slightly depressed at dorsal end, rostrum triangular with finely rounded tip, cucullus absent, and with seven caudal papillae present. The near full length 18S and 28S D2-D3 regions of rRNA genes sequences were characterised. The phylogenetic analyses revealed that the Fujian population of E. compsi grouped with the Zhejiang population of E. compsi, both being morphologically identical.

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Morphological Status ◽  
Group 3 ◽  
A New Species

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

Bursaphelenchus geraerti n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the United Arab Emirates

Nematology ◽  
2018 ◽  
Vol 20 (6) ◽  
pp. 583-595
Author(s):  
Xuan Wang ◽  
Munawar Maria ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Jincheng Wang ◽  
...  
Keyword(s):  
New Species ◽  
United Arab Emirates ◽  
Molecular Analysis ◽  
Excretory Pore ◽  
Posterior Margin ◽  
Nerve Ring ◽  
Lateral Field ◽  
Total Length

Summary Bursaphelenchus geraerti n. sp., isolated in Tianjin, P.R. China, from packaging wood made of Pinus sp. imported from the United Arab Emirates, is described. It is characterised by the lateral field with three lines, a stylet 13-14 μm long with small basal swellings, excretory pore located posterior to nerve ring, spicule mitten-shaped, ventrally arcuate with lamina smoothly tapering towards distal tip, with a distinct bend near 60% of total length, condylus elongated with rounded tip, rostrum conical with finely rounded tip, and cucullus weakly developed or absent. The bursal flap is wide and spade-like with the posterior margin truncate or rounded and starts posterior to P6 gland papillae. A vulval flap is absent and the tail is conical, strongly ventrally curved with the terminus pointed and sometimes appearing as a mucron. The new species is characterised by morphological and molecular analysis and is close to B. decraemerae, B. gerberae and B. sakishimanus.

Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan

Nematology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 335-346 ◽  
Author(s):  
Hongmei Li ◽  
Phap Quang Trinh ◽  
Lieven Waeyenberge ◽  
Maurice Moens
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Size ◽  
Posterior Margin ◽  
Petri Dish ◽  
Packaging Materials ◽  
Lateral Field ◽  
Female Tail ◽  
Domain Sequence ◽  
Lateral Lines

Abstract Bursaphelenchus chengi sp. n. is described and illustrated. Dauer juveniles were isolated from imported wood packaging materials from Taiwan to Nanjing Port, China. Bursaphelenchus chengi sp. n. was reared and maintained on Petri dish cultures of the fungus Botrytis cinerea. The new species is characterised by the medium body size in both sexes, the presence of only two incisures in the lateral field and the robust and strongly curved spicules. The spicule lamina is angular distally, the rostrum digitate and the condylus rounded. The tail is arcuate with a pointed terminus. The bursa is usually truncate with the posterior margin indented in some specimens or rounded with a fine axial point. Females have a small vulval flap formed by a short extension of the cuticle of the anterior lip, and a conical tail that gradually tapers to an almost straight or slightly recurved, pointed or rounded terminus. Because of the presence of two lateral lines, similar spicule shape, tapering female tail and the presence of a small vulval flap, B. chengi sp. n. should be grouped in the abietinus-group sensu Braasch. together with B. abietinus, B. antoniae, B. hellenicus, B. hylobianum and B. rainulfi. ITS-RFLP profiles support the proposal of the new species, and phylogenetic analysis of the 28S rDNA D2/D3 domain sequence places it close to B. antoniae and other species of the abietinus-group.

Phylogeny and redescription of Laimaphelenchus pannocaudus Massey, 1966 (Rhabditida: Aphelenchoididae) intercepted from Canadian Picea glauca

Nematology ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 199-212
Author(s):  
Yiwu Fang ◽  
Munawar Maria ◽  
Alcides Sánchez-Monge ◽  
Jianfeng Gu
Keyword(s):  
Electron Microscopy ◽  
Picea Glauca ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
The Body ◽  
Sister Relationship ◽  
Lateral Field ◽  
Female Tail ◽  
Partial 18S ◽  
Scanning Electron

Summary Laimaphelenchus pannocaudus, isolated in Ningbo port from Picea gluaca, is redescribed based upon characteristic morphological details elucidated by light and scanning electron microscopy photographs. The recovered population is characterised by 907 (771-1024) μm long females with 11.2 (8.9-12.2) μm long stylet, four incisures in the lateral field, excretory pore located at the same level as, or slightly anterior to the nerve ring, vulva lacking a flap and located at 68.7 (63.9-73.4)% of the body, elongate post-vulval uterine sac 78.5 (55.6-101.1) μm long, and tail 40.6 (33.4-45.6) μm long. Males are common and characterised by 16.5 (15.7-17.8) μm long spicules (chord) with their condylus slightly recurved, squared to rounded in shape with a blunt rounded tip and rostrum triangular with a bluntly pointed tip, and six visible caudal papillae. The female tail is ventrally curved, conoid and bears a stalk-like terminus with 4-8 pedunculate projections. Phylogenetic analyses using partial 18S and 28S rDNA D2-D3 data revealed that L. pannocaudus formed a sister relationship with L. suberensis in both phylogenies.

Bursaphelenchus pterocarpi n. sp. (Tylenchina: Aphelenchoididae) found in Pterocarpus sp. imported into China from Ghana

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 725-737
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Majid Pedram ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Reproductive System ◽  
Female Body ◽  
Nerve Ring ◽  
Wild Type ◽  
Lateral Field ◽  
Group Species ◽  
Partial 18S

Summary Bursaphelenchus pterocarpi n. sp. isolated from Pterocarpus sp. logs imported from Ghana to Ningbo, China, is described and illustrated. The new species is characterised by a female body length of 630-946 μm, lateral field with four lines, a short 12.6-13.9 μm long stylet with small basal swellings, excretory pore located posterior to nerve ring, outstretched reproductive system with amoeboid sperm inside the spermatheca, a well-developed vulval flap, tail conical and straight with a 1.9-4.8 μm long mucron at the tip, and lacking males in both the wild type and fungal-cultured populations. In a concatenated tree, reconstructed on the basis of the partial 18S, full length ITS and partial 28S rDNA D2-D3 sequences, the new species occupied a basal placement to the clade of xylophilus-group and africanus-group species.

Bursaphelenchus parayongensis n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from China

Nematology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Ute Schönfeld ◽  
Xinxin Ma ◽  
Xiaoling Lü
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Excretory Pore ◽  
Posterior Margin ◽  
Posterior Part ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
The Status ◽  
A New Species ◽  
Partial 18S

Summary Bursaphelenchus parayongensis n. sp. was isolated from packaging wood of a consignment from China exported to Germany. The new species is characterised by the lateral field with three lines, 9-13 μm long stylet with small basal swellings, and the excretory pore located near the posterior part of metacorpus. The male spicules are mitten-shaped, the lamina smoothly tapers towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular with sharply pointed tip, and without cucullus. One precloacal papilla, one adcloacal pair and two postcloacal pairs of male papillae present, with the posteriormost pair forming ‘gland papillae’. Bursal flap long, spade-like with posterior margin truncate or irregular. Females have a small vulval flap of 2-3 μm long, and a post-vulval uterine sac extending for 55-77% of the vulva to anus distance. Tail cylindrical, mostly (about 70%) with a robust mucron, very short to less than 3 μm, but occasionally tail terminus bluntly rounded, without mucron. It is similar to species in subgroup 3 of the eggersi group including B. carpini, B. clavicauda, B. cryphali and B. yongensis, but morphologically different. Detailed phylogenetic analysis based on partial 18S, ITS and D2-D3 region of 28S sequences has confirmed the status of this nematode as a new species.

Aphelenchoides smolae n. sp. (Tylenchina: Aphelenchoididae) found in Lilium orientalis imported into China from The Netherlands

Nematology ◽  
2020 ◽  
Vol 22 (7) ◽  
pp. 799-813
Author(s):  
Jingjing Cai ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Yiwu Fang ◽  
Hongmei Li
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
The Netherlands ◽  
Body Length ◽  
Excretory Pore ◽  
Female Body ◽  
Nerve Ring ◽  
Male Body ◽  
Lateral Field ◽  
Group 2

Summary Aphelenchoides smolae n. sp. isolated in Ningbo Port, China, from medium soil and tissues of Lilium orientalis bulbs imported from The Netherlands, is described. It is characterised by the female body length of 826 (672-1002) μm and male body length of 785 (683-844) μm, lateral field with four lines (three bands), stylet 13.0-14.9 μm long with small basal swellings, excretory pore situated slightly posterior to nerve ring, vulval flap absent, post-vulval uterine sac well developed and extending for ca 35.7-62.4% of vulva to anus distance, conical tail with terminus bearing a single mucron with many tiny nodular protuberances under SEM, males common, with spicules 25.7-29.8 μm long (median curved line), the condylus and rostrum rounded, well developed, the dorsal limb with a hook-like tip, and tail bearing three pairs (2 + 2 + 2) of caudal papillae and a sharply pointed mucron. The new species belongs to the Group 2 category of Aphelenchoides species sensu Shahina. Phylogenetic analysis based on 18S, 28S D2-D3 of rDNA and COI mtDNA further characterised the new species.

A KEY TO THE SPECIES OF THE NEMATODE GENUS APHELENCHOIDES FISCHER, 1894

1961 ◽  
Vol 39 (2) ◽  
pp. 143-148 ◽  
Author(s):  
K. C. Sanwal
Keyword(s):  
Excretory Pore ◽  
Nerve Ring ◽  
Line Drawings ◽  
Nematode Genus ◽  
Female Tail ◽  
Tail Structure

A key for the identification of 35 species of the nematode genus Aphelenchoides Fischer, 1894 is given. The key is based on the characters of the females. The chief characters used in the key are as follows: shape of female tail, structure of stylet, presence or absence of postvulvar uterine sac, position of excretory pore and nerve ring, and number of lateral incisures. The descriptive terms used in the key are illustrated by line drawings. A separate key is provided for species where only males are known.

Aphelenchoides medicagus n. sp. (Tylenchina: Aphelenchoididae) found in Medicago sativa imported into China from the USA

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 709-723 ◽  
Author(s):  
Zhen Wang ◽  
Wim Bert ◽  
Jianfeng Gu ◽  
Marjolein Couvreur ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Medicago Sativa ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male Tail ◽  
The Usa ◽  
Group 3 ◽  
A New Species

Summary Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μm long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μm long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis.

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