Morphological and molecular characterisation of Criconemoides brevistylus Singh & Khera, 1976 and C. obtusicaudatus Heyns, 1962 from South Africa (Nematoda: Criconematidae) with first description of a male C. obtusicaudatus and proposal of new synonyms

Nematology ◽  
2012 ◽  
Vol 14 (8) ◽  
pp. 961-976 ◽  
Author(s):  
Esther van den Berg ◽  
Louwrens R. Tiedt ◽  
Sergei A. Subbotin
Keyword(s):  
South Africa ◽  
Limpopo Province ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Closely Related Species ◽  
New Synonyms ◽  
Maize Field ◽  
Kwazulu Natal ◽  
First Time

During recent collections in South Africa, two species of Criconemoides were found. Criconemoides brevistylus is described and illustrated from a golf course in KwaZulu-Natal Province. It is compared with several closely related species, and three previously described species (C. helicus, C. onostris and C. paronostris) are regarded as junior synonyms of C. brevistylus. Criconemoides obtusicaudatus is described and illustrated from a maize field in Limpopo Province. The male of the species is described for the first time. Molecular characterisation of C. brevistylus and C. obtusicaudatus using the D2-D3 expansions segments of 28S rRNA and ITS rRNA gene sequences are provided. Phylogenetic relationships of these species with other Criconemoides are discussed.

Morphological and molecular characterisation of Hemicycliophora lutosa Loof & Heyns, 1969 and H. typica de Man, 1921 from South Africa (Nematoda: Hemicycliophoridae)

Nematology ◽  
2010 ◽  
Vol 12 (2) ◽  
pp. 303-308 ◽  
Author(s):  
Esther van den Berg ◽  
Sergei A. Subbotin ◽  
Louwrens R. Tiedt
Keyword(s):  
South Africa ◽  
Phylogenetic Relationships ◽  
Restriction Enzymes ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Diagnostic Pcr ◽  
Fallow Soil ◽  
De Man ◽  
First Time

AbstractTwo Hemicycliophora species, H. lutosa and H. typica, found in samples from fallow soil and sugarcane soil in South Africa, were studied morphologically and, for the first time, molecularly. Diagnostic PCR-IT-rRNA-RFLP profiles generated by five restriction enzymes are provided. Study of phylogenetic relationships using D2-D3 expansion segment of 28S rRNA gene sequences revealed that H. lutosa was related to H. poranga. Hemicycliophora lutosa and H. poranga are compared morphologically. SEM photographs are given for H. typica and for H. lutosa for the first time. The male of H. typica represents a first report for South Africa.

Morphological and molecular characterisation of three known species of Criconematoidea from Iran

Nematology ◽  
2020 ◽  
Vol 22 (7) ◽  
pp. 745-758
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Reza Ghaderi
Keyword(s):  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Morphometric Data ◽  
Fourth Stage ◽  
Closely Related Species ◽  
Morphometric Characters ◽  
Tail Shape ◽  
Lateral Lines ◽  
Stage Juvenile

Summary In this study, we provide morphological and molecular characterisation of three known species of the Criconematoidea, namely Criconema cylindraceum, Paratylenchus alleni and P. steineri, recovered from Iran. Descriptions, morphometric data, drawings and photomicrographs are provided, and comparisons are made with closely related species. Phylogenetic analysis using the D2-D3 expansion segments of the 28S rRNA gene revealed that C. cylindraceum formed a cluster with Ogma decalineatus, a species with a similar head structure, but with differences in cuticle ornamentation, tail shape and morphometric characters. Moreover, P. alleni grouped with species of Paratylenchus having four lateral lines and a short stylet (< 40 μm), exceptionally including the unresolved P. straeleni, while P. steineri formed a clade with long stylet species, including P. teres and P. wuae. The taxonomic placement of C. cylindraceum in Criconema, rather than in Criconemoides, is justified, and P. teres is molecularly differentiated from P. steineri, the two species differing in having or lacking a stylet in the fourth-stage juvenile.

Morphological and molecular characterisation of Caloosia longicaudata (Loos, 1948) Siddiqi & Goodey, 1963 (Nematoda: Caloosiidae) from Maui, the Hawaiian Islands with notes on some species of the genus

Nematology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 381-393 ◽  
Author(s):  
Esther van den Berg ◽  
Sergei Subbotin ◽  
Louwrens Tiedt
Keyword(s):  
18S Rrna ◽  
Hawaiian Islands ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Diagnostic Pcr ◽  
Rflp Profile ◽  
First Time ◽  
Partial 18S ◽  
Scanning Electron

AbstractCaloosia longicaudata is described from Maui, the Hawaiian Islands, for the first time and both sexes are characterised morphologically using light and scanning electron microscopy. Molecular characterisation of C. longicaudata using the D2-D3 domain of 28S rRNA, partial 18S rRNA and ITS rRNA gene sequences is also provided. The phylogenetic relationships of this species with other representatives of the suborder Criconematina are presented and discussed. A diagnostic PCR-ITS-RFLP profile for C. longicaudata is given together with an identification table for eight species of Caloosia. Caloosia langola n. comb. is transferred to the genus and C. shorai is synonymised with H. psidii.

Morphological and molecular characterisation of one new and several known species of the reniform nematode, Rotylenchulus Linford & Oliveira, 1940 (Hoplolaimidae: Rotylenchulinae), and a phylogeny of the genus

Nematology ◽  
2016 ◽  
Vol 18 (1) ◽  
pp. 67-107 ◽  
Author(s):  
Esther Van Den Berg ◽  
Juan E. Palomares-Rius ◽  
Nicola Vovlas ◽  
Louwrens R. Tiedt ◽  
Pablo Castillo ◽  
...  
Keyword(s):  
South Africa ◽  
Valid Species ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Rapid Diagnostics ◽  
Sister Relationship ◽  
Species Specific ◽  
Reniform Nematodes ◽  
A New Species

The reniform nematodes of the genusRotylenchulusare semi-endoparasites of numerous herbaceous and woody plant roots and are mainly distributed in tropical and subtropical regions. In this study, we provide morphological and molecular characterisation of six out of ten presently known valid species ofRotylenchulus:R. clavicaudatus,R. leptus,R. macrodoratus,R. macrosoma,R. reniformisandR. saccharifrom South Africa, USA, Italy and Spain.Rotylenchulus parvuswas only studied morphologically. A new species,R. macrosomoidessp. n., isolated from soil and roots of sugarcane in South Africa, is described. The phylogeny ofRotylenchulus, as inferred from the analyses of D2-D3 of 28S rRNA, ITS rRNA,coxImtDNA andhsp90gene sequences, is presented. The study revealed thatR. reniformisandR. macrosomahave a sister relationship, but that relationships between otherRotylenchulusspecies remain unresolved. The phylogenetic analysis also confirmed the hypothesis that this genus originated from the Afrotropical zoogeographical region. Our study revealed thatR. reniformisandR. macrosomoidessp. n. have two distinct rRNA gene types andR. macrosomahave three rRNA gene types in their genomes. PCR with species-specific primers was developed for rapid diagnostics ofR. reniformis.

Morphological and molecular characterisation of several Paratylenchus Micoletzky, 1922 (Tylenchida: Paratylenchidae) species from South Africa and USA, together with some taxonomic notes

Nematology ◽  
2014 ◽  
Vol 16 (3) ◽  
pp. 323-358 ◽  
Author(s):  
Esther Van den Berg ◽  
Esther Van den Berg ◽  
Louwrens R. Tiedt ◽  
Esther Van den Berg ◽  
Louwrens R. Tiedt ◽  
...  
Keyword(s):  
South Africa ◽  
Rrna Genes ◽  
Morphological Identification ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Electron Microscopic ◽  
Wide Range ◽  
Its Rrna Gene ◽  
Its Rrna

Pin nematodes of the genus Paratylenchus are widely distributed across the world and associated with many plant species. Morphological identification of Paratylenchus species is a difficult task because it relies on many characters with a wide range of intraspecific variation. In this study we provide morphological and molecular characterisation of several pin nematodes: Paratylenchus aquaticus, P. dianthus, P. hamatus, P. nanus and P. straeleni, collected in different states of the USA and South Africa. Paratylenchus aquaticus is reported from South Africa and Hawaii and P. nanus is found from South Africa for the first time. Morphological descriptions, morphometrics, light and scanning electron microscopic photos and drawings are given for these species. Molecular characterisation of nematodes using the D2-D3 of 28S rRNA and ITS rRNA gene sequence revealed that samples morphologically identified as P. aquaticus, P. hamatus and P. nanus indeed represent species complexes containing several species. Sequences of the rRNA genes are also provided for several unidentified Paratylenchus. Phylogenetic relationships within the genus Paratylenchus are given as inferred from the analyses of the D2-D3 of 28S rRNA and ITS rRNA gene sequences. We present here the most complete phylogenetic analysis of the genus.

New host-plants records of Afrotropical Gelechiidae (Lepidoptera), with description of three new species

Zootaxa ◽  
2021 ◽  
Vol 4952 (3) ◽  
pp. 495-522
Author(s):  
OLEKSIY BIDZILYA
Keyword(s):  
South Africa ◽  
First Record ◽  
Limpopo Province ◽  
New Combination ◽  
Larval Biology ◽  
New Host ◽  
North West ◽  
Annotated List ◽  
Three New Species ◽  
First Time

First and additional host-plant records for 38 species of Gelechiidae from South Africa and Kenya are presented. An annotated list with brief descriptions of larval biology and known geographical distributions is presented. Three species are described as new: Mesophleps kruegeri sp. nov. (Namibia: Kavango Region and South Africa: Limpopo Province), Istrianis inquilinus sp. nov. (South Africa: North West Province), and Teleiopsis sharporum sp. nov. (South Africa: Limpopo Province). The new combination Istrianis epacria (Bradley, 1965) comb. nov. is proposed. The male genitalia of Hypatima melanecta (Meyrick, 1914) is described for the first time. It is noticed that male hitherto associated with T. commaculata (Meyrick, 1918) in fact refers to T. pundamilia Bidzilya & Mey, 2018. Hypatima stasimodes (Meyrick, 1931), Dichomeris coenulenta (Meyrick, 1927), Dichomeris eustacta Meyrick, 1921, Neotelphusa similella Janse, 1958 and Argophara epaxia Janse, 1963 are recorded for the first time from South Africa, and Istrianis epacria is new for Kenya. The adults, male and female genitalia are illustrated for some species mentioned in paper. The first record of the genus Teleiopsis Sattler, 1960 from South Africa is briefly discussed. 

Morphological and molecular characterisation of Helicotylenchus ciceri n. sp. and H. scoticus Boag & Jairajpuri, 1985 (Nematoda: Hoplolaimidae) from Iran

Nematology ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 611-626
Author(s):  
Fariba Mohammadi Zameleh ◽  
Akbar Karegar ◽  
Reza Ghaderi ◽  
Abbas Mokaram Hesar
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
Rrna Genes ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Closely Related Species ◽  
Partial 18S ◽  
Molecular Characters

Summary Helicotylenchus ciceri n. sp. and H. scoticus are described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by a conical and truncated lip region with five or six distinct annuli, stylet 32-37 μm long with anteriorly concave knobs, secretory-excretory pore posterior to the pharyngo-intestinal valve, dorsally convex-conoid tail with a terminal projection, phasmids 14 (7-20) annuli anterior to the level of anus, empty spermatheca and absence of males. Intraspecific variation of 16 populations of H. scoticus, collected from chickpea and lentil fields in Kermanshah province, western Iran, is discussed. The results of the phylogenetic analyses based on the sequences of the partial 18S rRNA, D2-D3 expansion segments of 28S rRNA and ITS rRNA genes are provided for the studied species, confirming their differences from each other and determining the position of them and their relationships with closely related species.

Revision of the South African genus Neopimus (Coleoptera: Staphylinidae: Paederinae)

Zootaxa ◽  
2019 ◽  
Vol 4577 (2) ◽  
pp. 361
Author(s):  
JIŘÍ JANÁK
Keyword(s):  
South Africa ◽  
South African ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
The South ◽  
Additional Material ◽  
Kwazulu Natal ◽  
First Time ◽  
African Genus

A revision of the south African genus Neopimus Özdikmen, Demir & Türkeş, 2008 is presented. Based on revision of the type and additional material, three species are recognised. The genus Neopimus is redescribed and all species are described or redescribed and illustrated, two of them for the first time: Neopimus capensis Janák, sp. nov., from Eastern Cape Province, South Africa and N. zulu Janák, sp. nov., from KwaZulu-Natal Province, South Africa. The distribution of the genus is mapped and a key of species is presented. 

scholarly journals Rabies in South Africa between 1993 and 2005 - what has been achieved?

2010 ◽  
Vol 81 (1) ◽  
Author(s):  
B. Gummow ◽  
Y. A.A. Roefs ◽  
G. De Klerk
Keyword(s):  
South Africa ◽  
Domestic Animal ◽  
Limpopo Province ◽  
Domestic Dogs ◽  
Veterinary Services ◽  
Time And Space ◽  
Kwazulu Natal ◽  
Laboratory Results

Epidemiological analyses in time and space were carried out on all animal rabies cases reported in South Africa for the period 1993 - 2005. Validation of state veterinary records was done by comparing these with data from the 2 diagnostic laboratories mandated to test for animal rabies. A discrepancy between state veterinary records and laboratory results was found and is discussed. The total number of positive rabies cases reported to veterinary services between 1993 and 2005 was 4767. During this period the proportion of domestic animal rabies had increased to 79% (19% more than for the period 1980 - 1994), with 59% of the total cases being domestic dogs. Of the domestic animal cases 74% were canine and only 21% were bovine; when compared to the data from 1985 - 1994 there was an increase of 6% of rabies in canine and a 3% decrease in bovine cases. A disturbing trend is the increase in the incidence of rabies over the last 16 years in provinces neighbouring KwaZulu-Natal province, where rabies used to be concentrated, and in Limpopo province.

Morphological and molecular characterisation of Heterodera koreana (Vovlas, Lamberti & Choo, 1992) Mundo-Ocampo, Troccoli, Subbotin, Del Cid, Baldwin & Inserra, 2008 (Nematoda: Heteroderidae) from bamboo in Japan

Nematology ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 333-350 ◽  
Author(s):  
Shigeyuki Sekimoto ◽  
Taketo Uehara ◽  
Takayuki Mizukubo
Keyword(s):  
Phylogenetic Analyses ◽  
Original Description ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
Phyllostachys Edulis ◽  
Relationship Of ◽  
First Time

The Korean cyst nematode, Heterodera koreana, was recorded for the first time from Japan and characterised morphologically, morphometrically and molecularly. In total, 41 populations were detected from soil samples collected from the rhizosphere of four bamboo species in Japan: 31 populations from moso bamboo (Phyllostachys edulis), seven from madake (P. bambusoides), two from henon bamboo (P. nigra var. henonis) and one from fish pole bamboo (P. aurea). The morphology and morphometrics of the Japanese population were in agreement with those of the original description of H. koreana from South Korea and other subsequent descriptions from China and Iran, with the exception of some minor differences. The results of the phylogenetic analyses of the D2-D3 expansion segments of 28S rRNA gene and ITS rRNA gene sequences confirmed the species identification and phylogenetic relationship of H. koreana with other Heterodera species. The COI mtDNA gene sequences were obtained for the first time for H. koreana. Three COI haplotypes found in Japanese H. koreana populations showed a characteristic geographical distribution in Japan.

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