Species identity of Macrolophus melanotoma (Costa 1853) and Macrolophus pygmaeus (Rambur 1839) (Insecta: Heteroptera: Miridae) based on morphological and molecular data and bionomic implications

AbstractMacrolophus contains a small group of Palaearctic species with very simple and similar external morphology. The classification of these species has been based on variable characters such as body measurements, the height of the black band-shaped macula behind the eyes and the colour of the first antennal segment. Macrolophus melanotoma (Costa 1853), Macrolophus pygmaeus (Rambur 1839) and Macrolophus costalis Fieber 1858, are the most reputed predators of the genus. The classification history of M. melanotoma and M. pygmaeus shows a great number of misconceptions that have left the identity of the two species in confusion. Despite the economic importance of these two species, they have not received comprehensive taxonomical treatment until now. In this work, the morphological and sequence variation of a cytochrome b fragment (320 bp) were analysed to determine the identity of M. melanotoma and M. pygmaeus. Macrolophus costalis, Dicyphus cerastii Wagner 1951b, Dicyphus tamaninii Wagner 1951b, Cyrtopeltis geniculata Fieber 1861 and Nesidiocoris tenuis (Reuter 1895) were used as outgroup taxons in the phylogenetic analyses. Several evolutionary models were explored under a maximum likelihood framework. Macrolophus melanotoma and M. pygmaeus were classified as two distinct species based on monophyly, molecular and morphological data. Nodes at the species level were supported by high bootstrap values. M. pygmaeus and M. costalis are sister species, M. melanotoma basal to them. The shape of the black macula behind the eye may be used as a diagnostic character to differentiate M. melanotoma from M. pygmaeus with some degree of confidence.

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Description of a new species of Dicyphus Fieber (Insecta: Heteroptera: Miridae) from Portugal based on morphological and molecular data

Insect Systematics & Evolution ◽

10.1163/187631206788838572 ◽

2006 ◽

Vol 37 (3) ◽

pp. 281-300 ◽

Cited By ~ 7

Author(s):

Gerasimos Cassis ◽

Jose Isidro Martinez-Cascales ◽

Juan Antonio Sanchez

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Strong Support ◽

Molecular Data ◽

Species Identity ◽

Nesidiocoris Tenuis ◽

Dicyphus Tamaninii ◽

Morphological And Molecular Data ◽

Similar Pair ◽

A New Species

AbstractA new species of the plantbug genus Dicyphus (Insecta: Heteroptera: Miridae), D. umbertae Sanchez & Cassis sp. n., from Portugal is described and the closely related species, Dicyphus cerastii Wagner is redescribed. Several body measurements were taken. Male and female genitalia of both species are illustrated. D. umbertae was found in several localities in Central and South of Portugal on tomato (Lycopersicon esculentum Mill.) and Hyosciamus albus L. D. cerastii was found in southeast Spain on squash (Cucurbita maxima Duchesne), L. esculentum, Ononis natrix L., Withania frutescens (L.) Pauquy and H. albus. The external morphology of the two species is very alike and measurements overlap. In the two species the aedeagus has a similar pair of prominent slightly arcuate sclerites, but the shape and setae on the left paramere are different. Phylogenetic analyses were performed using 381 bp fragments of Cytochrome b. Other dicyphine species were used as outgroups in the analyses: Dicyphus tamaninii Wagner, D. rubicundus Blöte, D. escalerae Lindberg, Nesidiocoris tenuis (Reuter), Cyrtopeltis geniculata Fieber and Macrolophus sp. Several evolutionary models were explored under a maximum likelihood framework. The molecular analyses provided strong support for the species identity of the two sister species D. umbertae and D. cerastii.

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Analysis of the differences between Syntormon pallipes and S. pseudospicatus (Diptera: Dolichopodidae): morphological and molecular data

Zoosystematica Rossica ◽

10.31610/zsr/2019.28.2.305 ◽

2019 ◽

Vol 28 (2) ◽

pp. 305-316

Author(s):

M.A. Chursina ◽

I.Ya. Grichanov

Keyword(s):

Dna Analysis ◽

Distinct Species ◽

Molecular Data ◽

Wing Shape ◽

Morphological Data ◽

12S Rrna ◽

Separate Species ◽

Geometric Morphometric ◽

Geometric Morphometric Analysis ◽

Morphological And Molecular Data

The recent catalogues of the family Dolichopodidae considered Syntormon pallipes (Fabricius, 1794) and S. pseudospicatus Strobl, 1899 as separate species. In this study, we used three approaches to estimate the significance of differences between the two species: molecular analysis (COI and 12S rRNA sequences), analysis of leg colour characters and geometric morphometric analysis of wing shape. The morphological data confirmed the absence of significant differences between S. pallipes and S. pseudospicatus found in the DNA analysis. Significant differences in the wing shape of two species have not been revealed. Hence, according to our data, there is no reason to consider S. pseudospicatus as a distinct species.

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Redescription of Chimaera ogilbyi (Chimaeriformes; Chimaeridae) from the Indo-Australian region

Zootaxa ◽

10.11646/zootaxa.4375.2.2 ◽

2018 ◽

Vol 4375 (2) ◽

pp. 191 ◽

Cited By ~ 1

Author(s):

BRITTANY FINUCCI ◽

WILLIAM T. WHITE ◽

JENNY M. KEMPER ◽

GAVIN J.P. NAYLOR

Keyword(s):

Papua New Guinea ◽

Nuclear Dna ◽

Single Species ◽

Distinct Species ◽

Molecular Data ◽

Morphological Data ◽

Mitochondrial Markers ◽

Australian Region ◽

Taxonomic Approach ◽

Morphological And Molecular Data

An integrated taxonomic approach, combining both morphological and molecular data, was adopted to investigate the Hydrolagus lemures-ogilbyi group in the Indo-Australian region. Single mitochondrial markers (CO1 and NADH2) provided evidence supporting the separation of four distinct species in this group. However, detailed morphological data collected from specimens from across their range failed to find any consistent differences, and many features previously considered to be diagnostic were found to be variable. Nuclear DNA data also failed to support the differences found with the single mitochondrial markers and, together with the morphological data, supported the hypothesis that only a single species in this group is present in the Indo-Australian region. In addition, the results failed to support the current generic placement of this group in Hydrolagus, suggesting they belong to the genus Chimaera with doubt over the validity of Hydrolagus as a valid genus. The oldest available name for this group is Chimaera ogilbyi and a redescription is provided. This species occurs throughout Australia, eastern Indonesia (Java, Bali, and Lombok) and northern Papua New Guinea.

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Resurrection of Ornithogalum brevipedicellatum (Asparagaceae) with morphological and molecular data

Acta Botanica Croatica ◽

10.1515/botcro-2016-0001 ◽

2016 ◽

Vol 75 (1) ◽

pp. 60-66

Author(s):

Candan Aykurt ◽

İsmail G. Deniz ◽

Duygu Sari ◽

Mecit Vural ◽

Hüseyin Sümbül

Keyword(s):

Electron Microscopy ◽

Leaf Morphology ◽

Distinct Species ◽

Molecular Data ◽

Morphological Data ◽

Diagnostic Characteristics ◽

Molecular Studies ◽

Similarities And Differences ◽

Morphological And Molecular Data ◽

Scanning Electron

Abstract This study evaluates Ornithogalum brevipedicellatum, which was previously accepted as a synonym of O. oligophyllum, as a separate distinct species and discusses the similarities and differences between O. brevipedicellatum and its related species (O. oligophyllum and O. pamphylicum). Similarities and differences among these species were identifi ed by morphological and molecular studies. The leaf morphology and inflorescence of O. brevipedicellatum and O. pamphylicum are similar to each other, and in terms of these features, they show differences from O. oligophyllum. Some diagnostic characteristics are quite different in O. brevipedicellatum and O. pamphylicum, such as the size of tepals, length of fruiting pedicels and style. Morphological data were supported by the results obtained from molecular studies. According to a dendrogram obtained by molecular studies, O. brevipedicellatum and O. pamphylicum are similar. O. oligophyllum is more closely related to O. pyrenaicum used as an out-group. Additionally, the seeds of O. brevipedicellatum were examined with the use of scanning electron microscopy

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Silene sunhangii (Caryophyllaceae), a new species from China

PhytoKeys ◽

10.3897/phytokeys.135.36426 ◽

2019 ◽

Vol 135 ◽

pp. 59-69

Author(s):

Nan Lin ◽

Dai-Gui Zhang ◽

Xian-Han Huang ◽

Jian-Wen Zhang ◽

Jing-Yuan Yang ◽

...

Keyword(s):

New Species ◽

Taxonomic Status ◽

Its Region ◽

Distinct Species ◽

Molecular Data ◽

Central China ◽

Morphological Data ◽

Nuclear Its ◽

Morphological And Molecular Data ◽

A New Species

Silene sunhangii, a new species of Caryophyllaceae known from only three populations in Hubei and Hunan provinces of central China, is described. Both morphological and molecular data were used to assess the taxonomic status and relationships of this species. Morphologically, S. sunhangii is most similar to S. platyphylla Franch. from which it differs most readily in having 3-veined elliptical leaves without pubescence, tasseled catacorolla, pale purple to red petals without a linear lobe or narrow tooth and lanceolate, bifid to one third. A phylogenetic analysis based on nuclear ITS region identified the new species as a well-supported, independent lineage. Our new species is nested within a grade that encompasses species representing a polyphyletic Silene sect. Physolychnis (Benth.) Bocquet. Both the genetic and morphological data support the recognition of Silene sunhangii as a distinct species, although there is inconsistency between these two datasets as to the relationships of the new species.

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A new species of Fordiophyton (Sonerileae, Melastomataceae) from Yunnan, China

PhytoKeys ◽

10.3897/phytokeys.122.35260 ◽

2019 ◽

Vol 122 ◽

pp. 15-28 ◽

Cited By ~ 1

Author(s):

Jin-Hong Dai ◽

Qiu-Jie Zhou ◽

Zhi-Yong Yu ◽

Ren-Chao Zhou ◽

Ying Liu

Keyword(s):

Sequence Data ◽

Phylogenetic Analyses ◽

Leaf Size ◽

Distinct Species ◽

Molecular Data ◽

Morphological Evidence ◽

Flower Number ◽

Morphological And Molecular Data ◽

Nrits Sequence ◽

A New Species

Fordiophytonjinpingense (Melastomataceae; Sonerileae), a species occurring in south-eastern Yunnan, China, is described as new, based on morphological and molecular data. Phylogenetic analyses, based on nrITS sequence data, showed that, except F.breviscapum, all species sampled in Fordiophyton formed a strongly supported clade in which two geographical lineages were recovered. The generic placement of F.jinpingense is well supported by phylogenetic analyses and a character combination of 4-merous flowers, distinctly dimorphic stamens and the connectives basally not calcarate. Molecular divergence and morphological evidence indicate that F.jinpingense is well separated from other members of the genus, thus justifying its recognition as a distinct species. Fordiophytonjinpingense is phylogenetically closest to F.repens, but differs markedly from the latter in stem morphology (short, obtusely 4-sided vs. long, 4-angular), habit (erect vs. creeping), leaf size (6–16.5 × 4.5–13 cm vs. 4–7.5 × 4–6.5 cm) and flower number per inflorescence (5–13 vs. 3–6).

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Loose Ends in the Cortinarius Phylogeny: Five New Myxotelamonoid Species Indicate a High Diversity of These Ectomycorrhizal Fungi with South American Nothofagaceae

Life ◽

10.3390/life11050420 ◽

2021 ◽

Vol 11 (5) ◽

pp. 420

Author(s):

María Eugenia Salgado Salomón ◽

Carolina Barroetaveña ◽

Tuula Niskanen ◽

Kare Liimatainen ◽

Matthew E. Smith ◽

...

Keyword(s):

New Species ◽

Current Knowledge ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Global Scale ◽

South American ◽

Edible Fungi ◽

Rdna Its ◽

Morphological And Molecular Data ◽

High Diversity

This paper is a contribution to the current knowledge of taxonomy, ecology and distribution of South American Cortinarius (Pers.) Gray. Cortinarius is among the most widely distributed and species-rich basidiomycete genera occurring with South American Nothofagaceae and species are found in many distinct habitats, including shrublands and forests. Due to their ectomycorrhizal role, Cortinarius species are critical for nutrient cycling in forests, especially at higher latitudes. Some species have also been reported as edible fungi with high nutritional quality. Our aim is to unravel the taxonomy of selected Cortinarius belonging to phlegmacioid and myxotelamonioid species based on morphological and molecular data. After widely sampling Cortinarius specimens in Patagonian Nothofagaceae forests and comparing them to reference collections (including holotypes), we propose five new species of Cortinarius in this work. Phylogenetic analyses of concatenated rDNA ITS-LSU and RPB1 sequences failed to place these new species into known Cortinarius sections or lineages. These findings highlight our knowledge gaps regarding the fungal diversity of South American Nothofagaceae forests. Due to the high diversity of endemic Patagonian taxa, it is clear that the South American Cortinarius diversity needs to be discovered and described in order to understand the evolutionary history of Cortinarius on a global scale.

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Disentangling relationships among eastern Mediterranean Cymbalaria including description of a novel species from the southern Peloponnese (Greece)

Plant Systematics and Evolution ◽

10.1007/s00606-020-01730-3 ◽

2021 ◽

Vol 307 (2) ◽

Author(s):

Pau Carnicero ◽

Núria Garcia-Jacas ◽

Llorenç Sáez ◽

Theophanis Constantinidis ◽

Mercè Galbany-Casals

Keyword(s):

Eastern Mediterranean ◽

Phylogenetic Analyses ◽

Taxonomic Revision ◽

Distribution Patterns ◽

Morphological Characters ◽

Molecular Data ◽

Morphological Data ◽

Long Distance ◽

Aegean Islands ◽

Molecular Phylogenetic

AbstractThe eastern Mediterranean basin hosts a remarkably high plant diversity. Historical connections between currently isolated areas across the Aegean region and long-distance dispersal events have been invoked to explain current distribution patterns of species. According to most recent treatments, at least two Cymbalaria species occur in this area, Cymbalaria microcalyx and C. longipes. The former comprises several intraspecific taxa, treated at different ranks by different authors based on morphological data, evidencing the need of a taxonomic revision. Additionally, some populations of C. microcalyx show exclusive morphological characters that do not match any described taxon. Here, we aim to shed light on the systematics of eastern Mediterranean Cymbalaria and to propose a classification informed by various sources of evidence. We performed molecular phylogenetic analyses using ITS, 3’ETS, ndhF and rpl32-trnL sequences and estimated the ploidy level of some taxa performing relative genome size measures. Molecular data combined with morphology support the division of traditionally delimited C. microcalyx into C. acutiloba, C. microcalyx and C. minor, corresponding to well-delimited nrDNA lineages. Furthermore, we propose to combine C. microcalyx subsp. paradoxa at the species level. A group of specimens previously thought to belong to Cymbalaria microcalyx constitute a well-defined phylogenetic and morphological entity and are described here as a new species, Cymbalaria spetae. Cymbalaria longipes is non-monophyletic, but characterized by being glabrous and diploid, unlike other eastern species. The nrDNA data suggest at least two dispersals from the mainland to the Aegean Islands, potentially facilitated by marine regressions.

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Redescription of Paradiplozoon hemiculteri (Monogenea, Diplozoidae) from the type host Hemiculter leucisculus, with neotype designation

Parasite ◽

10.1051/parasite/2018004 ◽

2018 ◽

Vol 25 ◽

pp. 4 ◽

Cited By ~ 3

Author(s):

Dagmar Jirsová ◽

Xuejuan Ding ◽

Kristína Civáňová ◽

Eliška Jirounková ◽

Jana Ilgová ◽

...

Keyword(s):

Southern China ◽

Phylogenetic Analyses ◽

Posterior Part ◽

Original Description ◽

Molecular Data ◽

The Body ◽

Type Host ◽

Hemiculter Leucisculus ◽

Morphological And Molecular Data ◽

Median Plate

Paradiplozoon hemiculteri (Ling, 1973), a member of the Diplozoidae, parasitizes the gills of Asian fish. Not only is the type material unavailable for this species, the original description was poor and somewhat conflicting, and adequate molecular data were not available. What is more, the available morphological and molecular data are inconsistent and fluctuate significantly. Here, we present a redescription of P. hemiculteri based on morphological and molecular data from new isolates collected from the type host, the sharpbelly Hemiculter leucisculus (Basilewsky, 1855), captured at the neotype locality (Shaoguan, Guangdong Province, southern China); a neotype for P. hemiculteri was designated from this collection. The length and width of the body, buccal suckers, pharynx, attachment clamps, sickle and the central hook handle were all measured and the shape of the anterior and posterior part of the median plate and anterior and posterior joining sclerites accurately documented. Phylogenetic analyses based on the sequences of the second rDNA internal transcribed spacer (ITS2) indicated that all new samples clustered together and differed clearly from sequences attributed to P. hemiculteri, which are deposited in GenBank. Our results confirm that P. hemiculteri is the only diplozoid that has demonstrably been found on the gills of H. leucisculus to date.

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Morphological redescription and molecular characterization of Trichodina matsu Basson & Van As, 1994 (Ciliophora, Mobilida, Trichodinidae) infecting Tachysurus fulvidraco (Richardson, 1846) from Chongqing, China

Zootaxa ◽

10.11646/zootaxa.4995.2.6 ◽

2021 ◽

Vol 4995 (2) ◽

pp. 334-344

Author(s):

QIAN ZHOU ◽

FAHUI TANG ◽

YUANJUN ZHAO

Keyword(s):

Phylogenetic Analyses ◽

Molecular Data ◽

18S Rrna Gene ◽

Rrna Gene ◽

Similar Species ◽

Sequence Alignments ◽

Multiple Sequence ◽

Multiple Sequence Alignments ◽

Morphological And Molecular Data ◽

Tachysurus Fulvidraco

During a survey of parasitic ciliates in Chongqing, China, Trichodina matsu Basson & Van As, 1994 was isolated from gills of Tachysurus fulvidraco. Furthermore, the 18S rRNA gene and ITS-5.8S rRNA region of T. matsu were sequenced for the first time and applied for the species identification and comparison with similar species in the present study. Based on the morphological and molecular comparisons, the results indicate that T. matsu is an ectoparasite specific for the Siluriformes catfish. Based on the analyses of genetic distance, multiple sequence alignments, and phylogenetic analyses, no obvious differentiation within populations of T. matsu was found. In addition, the ‘Trichodina hyperparasitis’ (KX904933) in GenBank is a misidentification and appears to be conspecific with T. matsu according to the comparison of morphological and molecular data.

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