An unusual disjunction in Loasaceae: Central American Chichicaste grandis is nested in Brazilian Aosa

Phytotaxa ◽  
2018 ◽  
Vol 365 (3) ◽  
pp. 273 ◽  
Author(s):  
RAFAEL ACUÑA ◽  
ISLER F. CHINCHILLA ◽  
MAXIMILIAN WEIGEND
Keyword(s):  
Costa Rica ◽  
Single Species ◽  
Molecular Data ◽  
Central American ◽  
Morphological Data ◽  
New Combination ◽  
Nuclear Marker ◽  
Data Set ◽  
Plastid Markers ◽  
Morphological Differences

The highly distinctive genus Chichicaste is restricted to the Costa Rica-Chocó Biogeographic Hotspsot from Costa Rica to northwestern Colombia and comprises a single species, C. grandis. Relationships of this taxon have been doubtful in the absence of critical morphological analyses (particularly of living plants) and convincing molecular data. The present study aims at identifying the phylogenetic relationships of C. grandis using molecular and morphological data to establish its relationships. Our molecular data set includes four plastid markers (trnL–trnF, matK, trnS–trnG and rps16) and the nuclear marker ITS for 38 in-group taxa, including all of the currently recognized genera of the Loasoideae clade, and six out-group taxa from non Loasoideae Loasaceae and Hydrangeaceae. The dataset was analyzed using Maximum Likelihood and Bayesian Inference approaches. The plastid and nuclear trees were mostly congruent, with their respective ML best and BI strict consensus trees showing no significant differences in their topologies. Chichicaste is nested in Aosa series Parviflorae and sister to A. plumieri from Hispaniola, thus representing northern and western outliers of this otherwise strictly Brazilian genus. A critical morphological re-examination indicates that considering C. grandis as part of Aosa is plausible, in spite of the ecological and morphological differences between the two taxa. Based on these results the genus Chichicaste is synonymized with Aosa and the required new combination is provided. An amended key for an expanded Aosa, is also presented.

scholarly journals Combination of nuclear and mitochondrial markers as a useful tool to identify Ctenophthalmus species and subspecies (Siphonaptera: Ctenophthalmidae)

2021 ◽  
Author(s):  
Antonio Zurita ◽  
Cristina Cutillas
Keyword(s):  
Taxonomic Status ◽  
Molecular Study ◽  
Molecular Data ◽  
Point Of View ◽  
Morphological Data ◽  
Molecular Approach ◽  
Nuclear Marker ◽  
Mitochondrial Markers ◽  
Morphological Criteria ◽  
Mtdna Markers

AbstractCtenophthalmus is considered the largest genus within the Order Siphonaptera. From a morphological point of view, only males of this genus can be identified at species and subspecies levels using morphological keys, whereas there are no morphological criteria in order to classify females at these taxonomical levels. Furthermore, the amount of available molecular and phylogenetic data for this genus is quite scarce so far. The main objective of this work was to assess the utility of the combination of nuclear and mitochondrial markers with respect to their ability to differentiate among different subspecies within the Ctenophthalmus genus. With this purpose, we carried out a comparative morphological and molecular study of three different subspecies (Ctenophthalmus baeticus arvernus, Ctenophthalmus nobilis dobyi, and Ctenophthalmus andorrensis catalaniensis) in order to clarify and discuss its taxonomic status. In addition, our study complemented the molecular data previously provided for Ctenophthalmus baeticus boisseauorum and Ctenophthalmus apertus allani subspecies. We sequenced five different molecular markers: EF1-α, ITS1, ITS2, cox1, and cytb. Our results confirmed that morphological data by themselves are not able to discriminate among Ctenophthalmus female taxa; however, the combination of the nuclear marker EF1-α together with mtDNA markers cytb and cox1 constituted a useful taxonomical and phylogenetic tool to solve this issue. Based on these results, we consider that the use of this molecular approach should be gradually used within Ctenophthalmus genus in order to complement its classical taxonomy and clarifying the complex taxonomy of other congeneric species of fleas.

Redescription of the Central American genus Moribaetis Waltz & McCafferty 1985 (Ephemeroptera, Baetidae)

Zootaxa ◽  
2018 ◽  
Vol 4521 (2) ◽  
pp. 231
Author(s):  
NIKITA J. KLUGE ◽  
JUAN A. BERNAL VEGA
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Type Species ◽  
Central American ◽  
Type Locality ◽  
New Combination ◽  
Single Male ◽  
Male And Female ◽  
A New Species

A new definition for the genus Moribaetis Waltz & McCafferty 1985 is given. Its type species, Moribaetis maculipennis (Flowers 1979) is redescribed based on male and female imagoes reared from larvae near the type locality in Panama. Larvae, formerly wrongly attributed to Moribaetis salvini (Eaton 1885), and a male imago, formerly wrongly attributed to Moribaetis macaferti Waltz 1985, belong to a new species Moribaetis latipennis sp. n., which is described here based on a male imago reared from larva in Panama. Both species, M. maculipennis and M. latipennis sp. n., are distinct from M. salvini, which is known as a single male imago (lectotype) from Costa Rica. All other species, formerly attributed to Moribaetis, are excluded from this genus; a new combination Caribaetis macaferti comb. n. is proposed for the species originally described as Moribaetis macaferti Waltz (in Waltz & McCafferty) 1985, and a new combination Baetis (Rhodobaetis) mimbresaurus comb. n. is proposed for the species originally described as Moribaetis mimbresaurus McCafferty 2007. 

A revision of Australian species of Radula subg. Odontoradula

2013 ◽  
Vol 26 (6) ◽  
pp. 408 ◽  
Author(s):  
Matt A. M. Renner ◽  
Nicolas Devos ◽  
Elizabeth A. Brown ◽  
Matt J. von Konrat
Keyword(s):  
New Zealand ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
South Eastern ◽  
Chloroplast Markers ◽  
Molecular Divergence ◽  
Wet Tropics ◽  
Australian Flora ◽  
Morphological Differences

The current paper presents molecular data from three chloroplast markers (atpB–rbcL spacer, trnG G2 intron, trnL–trnF intron and spacer); morphological data, and geographic data to support the recognition of nine species belonging to Radula subg. Odontoradula in Australasia. R. ocellata, the subgeneric type from the Wet Tropics bioregion, is maintained as distinct from its sister species, R. pulchella, from south-eastern Australian rainforests; both species are Australian endemics. Reinstatement of R. allisonii from synonymy, under R. retroflexa, is supported by molecular data and morphological characters, including the absence of triradiate trigones on leaf-lobe cell walls, the apex of lobules on primary shoots not being turned outwards, the oblong-elliptic female bracts, and the perianths having a pronounced wing. Reinstatement of R. weymouthiana, from synonymy under R. retroflexa, is also supported by molecular data and morphological characters, including the presence of a single low dome-shaped papilla over each leaf-lobe cell, and the large imbricate lobules on primary shoots. R. weymouthiana occurs in Tasmania and New Zealand, whereas R. allisonii is a New Zealand endemic. Australian R. retroflexa exhibits differentiation into epiphytic and rheophytic morphs, interpreted as ecotypes. Australian individuals, comprising both epiphytic and rheophytic morphs, are monophyletic and nested within a clade containing individuals from other regions. R. novae-hollandiae is newly reported for the New Zealand Botanical Region, from Raoul Island in the Kermadecs. R. novae-hollandiae exhibits decoupling of morphological and molecular divergence, with Australian individuals forming two clades reflecting geography (a Wet Tropics bioregion clade and a south-eastern Rainforest clade). These clades exhibit equivalent levels of molecular divergence, as observed in R. pulchella and R. ocellata, but no morphological differences. Similar levels of molecular divergence were observed in trans-Tasman populations of R. tasmanica. The New Zealand endemic, R. plicata, is excluded from the Australian flora, and R. cuspidata replaces R. dentifolia for the New Zealand endemic species formerly known by both names.

A molecular phylogeny of Callianassidae and related families (Crustacea : Decapoda : Axiidea) with morphological support

2020 ◽  
Vol 34 (2) ◽  
pp. 113 ◽  
Author(s):  
Rafael Robles ◽  
Peter C. Dworschak ◽  
Darryl L. Felder ◽  
Gary C. B. Poore ◽  
Fernando L. Mantelatto
Keyword(s):  
Bayesian Analysis ◽  
Molecular Analysis ◽  
Single Species ◽  
Molecular Data ◽  
Total Evidence ◽  
Morphological Data ◽  
12S Rrna ◽  
New Genera ◽  
Key Species ◽  
The Family

The axiidean families Callianassidae and Ctenochelidae, sometimes treated together as Callianassoidea, are shown to represent a monophyletic taxon. It comprises 265 accepted species in 74 genera, twice this number of species if fossil taxa are included. The higher taxonomy of the group has proved difficult and fluid. In a molecular phylogenetic approach, we inferred evolutionary relationships from a maximum-likelihood (ML) and Bayesian analysis of four genes, mitochondrial 16S rRNA and 12S rRNA along with nuclear histone H3 and 18S rRNA. Our sample consisted of 298 specimens representing 123 species plus two species each of Axiidae and Callianideidae serving as outgroups. This number represented about half of all known species, but included 26 species undescribed or not confidently identified, 9% of all known. In a parallel morphological approach, the published descriptions of all species were examined and detailed observations made on about two-thirds of the known fauna in museum collections. A DELTA (Description Language for Taxonomy), database of 135 characters was made for 195 putative species, 18 of which were undescribed. A PAUP analysis found small clades coincident with the terminal clades found in the molecular treatment. Bayesian analysis of a total-evidence dataset combined elements of both molecular and morphological analyses. Clades were interpreted as seven families and 53 genera. Seventeen new genera are required to reflect the molecular and morphological phylograms. Relationships between the families and genera inferred from the two analyses differed between the two strategies in spite of retrospective searches for morphological features supporting intermediate clades. The family Ctenochelidae was recovered in both analyses but the monophyly of Paragourretia was not supported by molecular data. The hitherto well recognised family Eucalliacidae was found to be polyphyletic in the molecular analysis, but the family and its genera were well defined by morphological synapomorphies. The phylogram for Callianassidae suggested the isolation of several species from the genera to which they had traditionally been assigned and necessitated 12 new generic names. The same was true for Callichiridae, with stronger ML than Bayesian support, and five new genera are proposed. Morphological data did not reliably reflect generic relationships inferred from the molecular analysis though they did diagnose terminal taxa treated as genera. We conclude that discrepancies between molecular and morphological analyses are due at least in part to missing sequences for key species, but no less to our inability to recognise unambiguously informative morphological synapomorphies. The ML analysis revealed the presence of at least 10 complexes wherein 2–4 cryptic species masquerade under single species names.

Back to the Future? Molecules Take Us Back to the 1925 Classification of the Lembophyllaceae (Bryopsida)

2009 ◽  
Vol 34 (3) ◽  
pp. 443-454 ◽  
Author(s):  
Dietmar Quandt ◽  
Sanna Huttunen ◽  
Ray Tangney ◽  
Michael Stech
Keyword(s):  
Single Species ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
New Family ◽  
Morphological Studies ◽  
The Family ◽  
Family Level ◽  
Plastid Markers ◽  
Pleurocarpous Moss

Although the Lembophyllaceae has undergone considerable revision during the last century, the generic and familial level relationships of this pleurocarpous moss family are still poorly understood. To address this problem, a generic revision of the Lembophyllaceae based on molecular data was undertaken. We analyzed two plastid markers, the trnL-trnF and the psbT-psbH region in combination with the ITS2 of nuclear ribosomal DNA. The molecular data reveal that the current circumscription of the family is too narrow and that several genera previously placed in the Lembophyllaceae should be reincluded. The family includes: Bestia, Camptochaete, Dolichomitra, Dolichomitriopsis, Fallaciella, Fifea, Isothecium, Lembophyllum, Looseria stat. nov., Pilotrichella, Rigodium, Tripterocladium, and Weymouthia. Looseria contains a single species: Looseria orbiculata comb. nov. Acrocladium is excluded and provisionally accommodated in the Lepyrodontaceae. Generic limits supported by the molecular data support a return to the early twentieth century family concept of Brotherus. The analyses indicate that the segregate genus Orthostichella is distinct from its parent genus Pilotrichella, probably at the family level. Whereas Pilotrichella is resolved within the Lembophyllaceae, Orthostichella clusters with Porotrichum and Porothamnium forming a clade (OPP-clade) sister to the remaining Neckeraceae and Lembophyllaceae. Hence, the Neckeraceae is paraphyletic. Recognition of the OPP-clade as a new family is desirable but awaits the results of detailed ongoing morphological studies.

scholarly journals DNA Barcoding: Mixed results for big-headed flies (Diptera: Pipunculidae)

Zootaxa ◽  
2007 ◽  
Vol 1423 (1) ◽  
pp. 1-26 ◽  
Author(s):  
JEFFREY H. SKEVINGTON ◽  
CHRISTIAN KEHLMAIER ◽  
GUNILLA STÅHLS
Keyword(s):  
Dna Barcoding ◽  
Sequence Data ◽  
Phylogenetic Signal ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Base Pairs ◽  
Data Set ◽  
Taxonomic Decision ◽  
Morphological Species

Sequence data from 658 base pairs of mitochondrial cytochrome c oxidase I (cox1) were analysed for 28 described species of Pipunculidae (Diptera) in an effort to test the concept of DNA Barcoding on this family. Two recently revised but distantly related pipunculid lineages with presumed different evolutionary histories were used for the test (Clistoabdominalis Skevington, 2001 and Nephrocerus Zetterstedt, 1838). An effort was made to test the concept using sister taxa and morphologically similar sibling species swarms in these two genera. Morphological species concepts for Clistoabdominalis taxa were either supported by cox1 data or found to be too broad. Most of the discordance could be accounted for after reassessing morphological characters. In these cases, the molecular data were invaluable in assisting taxonomic decision-making. The radiation of Nearctic species of Nephrocerus could not be diagnosed using cox1. The ability of cox1 to recover phylogenetic signal was also tested on Clistoabdominalis. Morphological data for Clistoabdominalis were combined with the molecular data set. The pipunculid phylogeny from molecular data closely resembles the published phylogeny based on morphology. Partitioned Bremer support is used to localize areas of conflict between the datasets.

scholarly journals The phylogenetic conundrum of Lutzia (Diptera: Culicidae: Culicini): a cautionary account of conflict and support

2015 ◽  
Vol 46 (3) ◽  
pp. 269-290 ◽  
Author(s):  
Ian J. Kitching ◽  
C. Lorna Culverwell ◽  
Ralph E. Harbach
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Set ◽  
Generic Status ◽  
Strong Signal ◽  
Phylogenetic Studies ◽  
Data Support

Lutzia Theobald was reduced to a subgenus of Culex in 1932 and was treated as such until it was restored to its original generic status in 2003, based mainly on modifications of the larvae for predation. Previous phylogenetic studies based on morphological and molecular data have provided conflicting support for the generic status of Lutzia: analyses of morphological data support the generic status whereas analyses based on DNA sequences do not. Our previous phylogenetic analyses of Culicini (based on 169 morphological characters and 86 species representing the four genera and 26 subgenera of Culicini, most informal group taxa of subgenus Culex and five outgroup species from other tribes) seemed to indicate a conflict between adult and larval morphological data. Hence, we conducted a series of comparative and data exclusion analyses to determine whether the alternative positions of Lutzia are due to conflicting signal or to a lack of strong signal. We found that separate and combined analyses of adult and larval data support different patterns of relationships between Lutzia and other Culicini. However, the majority of conflicting clades are poorly supported and once these are removed from consideration, most of the topological disparity disappears, along with much of the resolution, suggesting that morphology alone does not have sufficiently strong signal to resolve the position of Lutzia. We critically examine the results of other phylogenetic studies of culicinine relationships and conclude that no morphological or molecular data set analysed in any study conducted to date has adequate signal to place Lutzia unequivocally with regard to other taxa in Culicini. Phylogenetic relationships observed thus far suggest that Lutzia is placed within Culex but further data and extended taxon sampling are required to confirm its position relative to Culex.

The greenhouse thrips, Heliothrips haemorrhoidalis, and its generic relationships within the subfamily Panchaetothripinae (Thysanoptera: Thripidae)

2001 ◽  
Vol 32 (2) ◽  
pp. 205-216 ◽  
Author(s):  
John W.H. Trueman ◽  
Rita Marullo ◽  
Laurence A. Mound
Keyword(s):  
Sister Group ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Sister Group Relationship ◽  
Pest Species ◽  
Data Set ◽  
Weak Support ◽  
Heliothrips Haemorrhoidalis ◽  
Generic Relationships

AbstractThe subfamily Panchaetothripinae, comprising 35 genera and 98 species, includes several pest species of which the most notorious is the greenhouse thrips, Heliothrips haemorrhoidalis. In an attempt to establish the sister-group of Heliothrips, the relationships of this genus to 31 of the other genera in the subfamily were examined cladistically, using 35 parsimony-informative morphological characters. The analysis indicated that there was no support for two of the three tribes into which this subfamily is customarily arranged, the Monilothripini and the Panchaetothripini, but weak support for the tribe Tryphactothripini. No clear sister-group relationship could be identified for the New World genus Heliothrips, although it grouped with three old world genera Australothrips, Retithrips and Rhipiphorothrips. It is concluded that a morphological data set is not capable of producing a robust phylogeny of the Panchaetothripinae, and that the subject requires re-examination using molecular data.

The impact of multiple molecular and morphological data sets on the phylogenetic reconstruction of subtribe Neurachninae (Poaceae: Panicoideae: Paniceae)

2021 ◽  
Author(s):  
E. J. Thompson ◽  
Melodina Fabillo
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Reconstruction ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Sets ◽  
Plastid Markers ◽  
Morphological And Molecular Data ◽  
The Impact ◽  
Selection Of

The taxonomy of Neurachninane has been unstable, with its member genera consisting of Ancistrachne, Calyptochloa, Cleistochloa, Dimorphochloa, Neurachne, Paraneurachne and Thyridolepis, changing since its original circumscription that comprised only the latter three genera. Recent studies on the phylogeny of Neurachninae have focused primarily on molecular data. We analysed the phylogeny of Neurachninae on the basis of molecular data from seven molecular loci (plastid markers: matK, ndhF, rbcL, rpl16, rpoC2 and trnLF, and ribosomal internal transcribed spacer, ITS) and morphological data from 104 morphological characters, including new taxonomically informative micromorphology of upper paleas. We devised an impact assessment scoring (IAS) protocol to aid selection of a tree for inferring the phylogeny of Neurachninae. Combining morphological and molecular data resulted in a well resolved phylogeny with the highest IAS value. Our findings support reinstatement of subtribe Neurachninae in its original sense, Neurachne muelleri and Dimorphochloa rigida. We show that Ancistrachne, Cleistochloa and Dimorphochloa are not monophyletic and Ancistrachne maidenii, Calyptochloa, Cleistochloa and Dimorphochloa form a new group, the cleistogamy group, united by having unique morphology associated with reproductive dimorphism.

Phylogenetic analysis of the druid flies (Diptera: Schizophora: Clusiidae) based on morphological and molecular data

2010 ◽  
Vol 41 (3) ◽  
pp. 231-274 ◽  
Author(s):  
Jinzhong Fu ◽  
Owen Lonsdale ◽  
Brian Wiegmann ◽  
Stephen Marshall
Keyword(s):  
Cytochrome Oxidase ◽  
Dna Sequences ◽  
Phylogenetic Reconstruction ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Sets ◽  
Cytochrome Oxidase Subunit ◽  
Data Set ◽  
Oxidase Subunit ◽  
Morphological And Molecular Data

AbstractIn this paper, the Clusiidae (Diptera: Schizophora) is analyzed phylogenetically using morphological and molecular data sets, and then redefined on the basis of derived morphological characters. The biology and distribution of the Clusiidae are also reviewed, a key is provided to the World genera, the status of the genus Craspedochaeta Czerny is reevaluated and the type of Heterochroa pictipennis Wulp is discussed. Molecular data sets include genomic DNA sequences from the mitochondrial genes COI (cytochrome oxidase subunit I) and COII (cytochrome oxidase subunit II), the large ribosomal nuclear subunit 28S, and the nuclear protein-coding carbomoylphosphate synthase (CPS) domain of CAD (or “rudimentary”). Genes were analyzed separately, in combination with each other, and in combination with a morphological data set. Although individual molecular data sets often provided conflicting phylogenetic signals, the topologies of the cladograms produced from each data set alone or in combination were largely similar. Most genus-level relationships and several basal divergences were unresolved, but Apiochaeta was very strongly and consistently supported as Sobarocephalinae, not Clusiinae. The Clusiinae and Sobarocephalinae are subsequently redefined using an adjusted morphological tree — retaining Apiochaeta in the Sobarocephalinae — that is only slightly longer (8.4%, or seven steps) than the most parsimonious tree. Our results illustrate the benefits of multiple independent data sets for phylogenetic reconstruction in order to verify and refine existing classifications.

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