Boeremia parva sp. nov., a novel species of the family Didymellaceae isolated from soil

Phytotaxa ◽  
2021 ◽  
Vol 518 (1) ◽  
pp. 25-35
Author(s):  
KALLOL DAS ◽  
LEONID N. TEN ◽  
JAE-HO BAN ◽  
SEUNG-YEOL LEE ◽  
HEE-YOUNG JUNG
Keyword(s):  
Dna Sequences ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Phylogenetic Position ◽  
The Novel ◽  
Closely Related Species ◽  
The Family ◽  
Colony Color ◽  
Average Size ◽  
Isolated Species

Fungal strains, designated KNU-NL4 and KNU-OL2, belonging to the family Didymellaceae were isolated from a soil sample collected in Miryang, Korea. Phylogenetic analyses based on a concatenated dataset of DNA sequences of ITS regions and partial sequences of ACT, CAL, TEF1-α, and β-TUB genes showed that the isolates reside in a clade together with Boeremia species but occupy the distinct phylogenetic position. Morphologically, the novel strains produce bigger conidiomata (average size 169.8 μm) than the closely related B. rhapontica (126.59 μm) and smaller than the other close neighbor B. coffeae (187.5 μm). Both novel strains also differed from them by smaller colony size and colony color on OA and MEA. The detailed descriptions, illustrations, and discussions regarding the morphological and phylogenetic analyses of the closely related species are provided to support the novelty of the isolated species. The results of phylogenetic analysis and morphological observations indicate that strains KNU-NL4 and KNU-OL2 represent a novel species in the genus Boeremia, for which the name Boeremia parva sp. nov. is proposed.

Diplodia parva sp. nov., a novel species of the family Botryosphaeriaceae isolated from soil in Korea

Phytotaxa ◽  
2021 ◽  
Vol 514 (2) ◽  
pp. 105-117
Author(s):  
SEUNG-YEOL LEE ◽  
LEONID N. TEN ◽  
BENJAMIN YAW AYIM ◽  
OKOUMA NGUIA FULBERT ◽  
KALLOL DAS ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Related Species ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Fungal Strain ◽  
Phylogenetic Position ◽  
Closely Related Species ◽  
The Family ◽  
Morphological Differences ◽  
Isolated Species

A fungal strain designated KNU16-007, belonging to the family Botryosphaeriaceae, was isolated from soil in Daegu, Korea. Phylogenetic analyses based on the concatenated nucleotide sequences of the ITS and TEF-1α regions, showed that the isolate resides in a clade together with Diplodia species but occupies a distinct phylogenetic position. Conidial dimensions of strain KNU16-007 (22.7–29.3 μm × 8.9–10.9 μm) or its conidial length:width ratio were significantly differed from those of the closely related D. sapinea, D. intermedia, D. scrobiculata, D. seriata, D. crataegicola, D. rosacearum, and D. citricarpa clearly indicating morphological differences from these species. Detailed descriptions, illustrations, and discussions regarding the morphological and phylogenetic analyses of the closely related species are provided to support the novelty of the isolated species. The results of phylogenetic analysis and morphological observations indicate that strain KNU16-007 represents a novel species in the genus Diplodia, for which the name Diplodia parva sp. nov. is proposed.

scholarly journals Pelagicoccus croceus sp. nov., a novel marine member of the family Puniceicoccaceae within the phylum ‘Verrucomicrobia’ isolated from seagrass

2007 ◽  
Vol 57 (12) ◽  
pp. 2874-2880 ◽  
Author(s):  
Jaewoo Yoon ◽  
Naoya Oku ◽  
Satoru Matsuda ◽  
Hiroaki Kasai ◽  
Akira Yokota
Keyword(s):  
Cell Wall ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Antibiotic Susceptibility Test ◽  
The Family ◽  
Lactam Antibiotic

An obligately aerobic, spherical, non-motile, pale-yellow pigmented bacterium was isolated from a piece of leaf of seagrass, Enhalus acoroides (L.f.) Royle, grown in Okinawa, Japan and was subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate N5FB36-5T shared approximately 96–98 % sequence similarity with the species of the genus Pelagicoccus of the family Puniceicoccaceae within the phylum ‘Verrucomicrobia’. The DNA–DNA relatedness values of strain N5FB36-5T with Pelagicoccus mobilis 02PA-Ca-133T and Pelagicoccus albus YM14-201T were below 70 %, which is accepted as the phylogenetic definition of a novel species. β-Lactam antibiotic susceptibility test and amino acid analysis of the cell wall hydrolysates indicated the absence of muramic acid and diaminopimelic acid in the cell walls, which suggested that this strain lacks an ordinary Gram-negative type of peptidoglycan in the cell wall. The DNA G+C content of strain N5FB36-5T was 51.6 mol%; MK-7 was the major menaquinone; and the presence of C16 : 0, C16 : 1 ω7c and anteiso-C15 : 0 as the major cellular fatty acids supported the identification of the novel isolate as a member of the genus Pelagicoccus. On the basis of polyphasic taxonomic data, it was concluded that this strain should be classified as a novel species of the genus Pelagicoccus, for which the name Pelagicoccus croceus sp. nov. is proposed. The type strain is N5FB36-5T (=MBIC08282T=KCTC 12903T).

scholarly journals  Azygosporus gen. nov., a synapmorphic clade in the family Ancylistaceae

MycoKeys ◽  
2021 ◽  
Vol 85 ◽  
pp. 161-172
Author(s):  
Yue Cai ◽  
Yong Nie ◽  
Heng Zhao ◽  
ZiMin Wang ◽  
ZhengYu Zhou ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
New Combination ◽  
Molecular Evidence ◽  
The Novel ◽  
Closely Related Species ◽  
The Family ◽  
Distinct Lineage ◽  
A New Species ◽  
Taxonomic System

The fungal genus Conidiobolus sensu lato was delimited into four genera based on morphology and phylogeny. However, the taxonomic placement of C. parvus has not been determined until now. Here, we show that C. parvus belongs to a distinct lineage based on mitochondrial (mtSSU) and nuclear (TEF1 and nrLSU) phylogenetic analyses. Phylogenetic analyses further revealed a new species as sister to C. parvus. We identified a synapomorphy uniting these lineages (azygospore production) that was not observed in other allied genera of the family Ancylistaceae, and erected a new genus Azygosporusgen. nov. for this monophyletic group, with a new combination, A. parvuscomb. nov. as the type species. Within Azygosporus, the novel species A. macropapillatussp. nov. was introduced from China based on morphological characteristics and molecular evidence, which is characterized by its prominent basal papilla, in comparison to other closely related species, measuring 7.5–10.0×5.0–10.0 µm. Our study resolved the phylogenetic placement of C. parvus and improved the taxonomic system of the Ancylistaceae family.

scholarly journals Rosistilla oblonga gen. nov., sp. nov. and Rosistilla carotiformis sp. nov., isolated from biotic or abiotic surfaces in Northern Germany, Mallorca, Spain and California, USA

2020 ◽  
Vol 113 (12) ◽  
pp. 1939-1952 ◽  
Author(s):  
Muhammad Waqqas ◽  
Markus Salbreiter ◽  
Nicolai Kallscheuer ◽  
Mareike Jogler ◽  
Sandra Wiegand ◽  
...  
Keyword(s):  
Cell Biology ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
The Novel ◽  
Biological Features ◽  
Abiotic Surfaces ◽  
The Family ◽  
Axenic Cultures ◽  
The Baltic

AbstractPlanctomycetes are ubiquitous bacteria with fascinating cell biological features. Strains available as axenic cultures in most cases have been isolated from aquatic environments and serve as a basis to study planctomycetal cell biology and interactions in further detail. As a contribution to the current collection of axenic cultures, here we characterise three closely related strains, Poly24T, CA51T and Mal33, which were isolated from the Baltic Sea, the Pacific Ocean and the Mediterranean Sea, respectively. The strains display cell biological features typical for related Planctomycetes, such as division by polar budding, presence of crateriform structures and formation of rosettes. Optimal growth was observed at temperatures of 30–33 °C and at pH 7.5, which led to maximal growth rates of 0.065–0.079 h−1, corresponding to generation times of 9–11 h. The genomes of the novel isolates have a size of 7.3–7.5 Mb and a G + C content of 57.7–58.2%. Phylogenetic analyses place the strains in the family Pirellulaceae and suggest that Roseimaritima ulvae and Roseimaritima sediminicola are the current closest relatives. Analysis of five different phylogenetic markers, however, supports the delineation of the strains from members of the genus Roseimaritima and other characterised genera in the family. Supported by morphological and physiological differences, we conclude that the strains belong to the novel genus Rosistilla gen. nov. and constitute two novel species, for which we propose the names Rosistilla carotiformis sp. nov. and Rosistilla oblonga sp. nov. (the type species). The two novel species are represented by the type strains Poly24T (= DSM 102938T = VKM B-3434T = LMG 31347T = CECT 9848T) and CA51T (= DSM 104080T = LMG 29702T), respectively.

scholarly journals Cryolevonia gen. nov. and Cryolevonia schafbergensis sp. nov., a cryophilic yeast from ancient permafrost and melted sea ice

2020 ◽  
Vol 70 (4) ◽  
pp. 2334-2338 ◽  
Author(s):  
Ana Pontes ◽  
Joel Ruethi ◽  
Beat Frey ◽  
Andreia Aires ◽  
Amanda Thomas ◽  
...  
Keyword(s):  
Sea Ice ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Its Region ◽  
Swiss Alps ◽  
Sexual Cycle ◽  
The Novel ◽  
Prolonged Incubation ◽  
D2 Domain ◽  
The Family

A cryophilic basidiomycetous yeast unable to grow at 18 °C or higher temperatures was isolated from a subsurface permafrost layer collected in the Eastern Swiss Alps and from melted sea ice collected in the Artic at Frobisher Bay, Nunavut, Canada. Phylogenetic analyses employing combined sequences of the D1/D2 domain and ITS region indicated that the two new isolates belong to the family Camptobasidiaceae of the class Microbotryomycetes but are distantly related to any of the currently recognized species and genera. Consequently, the novel genus Cryolevonia, and the novel species Cryolevonia schafbergensis (type strain PYCC 8347T=CBS 16055T) are proposed to accommodate this cryophilic yeast. Although sparse hyphae and teliospore-like stuctures were observed upon prolonged incubation, a sexual cycle was not observed and therefore C. schafbergensis is documented solely from its asexual stage.

scholarly journals First records and a new genus of comb-tailed spiders (Araneae: Hahniidae) from Thailand with comments on the six-eyed species of this family

2020 ◽  
Vol 724 ◽  
Author(s):  
Francisco Andrés Rivera-Quiroz ◽  
Booppa Petcharad ◽  
Jeremy A. Miller
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
New Genus ◽  
New Combination ◽  
Phylogenetic Position ◽  
The Novel ◽  
Novel Genus ◽  
The Family ◽  
Two New Species ◽  
First Time

The family Hahniidae is reported from Thailand for the first time. The genus Hexamatia gen. nov. and two new species, Hexamatia seekhaow gen. et sp. nov. and Hahnia ngai sp. nov., are described and illustrated. DNA sequences are provided for all the species reported here. The phylogenetic position of the novel genus Hexamatia gen. nov. and its relation to Hahnia are discussed. Based on these results, a new combination is proposed for Hexamatia senaria (Zhang, Li & Zheng, 2011) gen. et comb. nov. = Hahnia senaria. Known distribution of the species Hahnia saccata Zhang, Li & Zheng, 2011, originally described from China, is expanded. A brief review and notes on the taxonomy of the six-eyed hahniids are included.

scholarly journals Description and phylogenetic position of Corlissina maricaensis gen. nov., sp. nov. (Karyorelictea, Geleiidae), a novel interstitial ciliate from Brazil, with redefinition of the family Geleiidae

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4297-4308 ◽  
Author(s):  
Pedro H. Campello-Nunes ◽  
Noemi Fernandes ◽  
Martin Schlegel ◽  
Inácio D. Silva-Neto
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Cylindrical Body ◽  
Sister Group ◽  
18S Rrna Gene ◽  
Phylogenetic Position ◽  
Morphological Description ◽  
Rrna Gene ◽  
The Novel ◽  
Cortical Granules

Corlissina maricaensis gen. nov., sp. nov. was obtained from samples of sediment collected in a brackish lagoon of Maricá city, Rio de Janeiro state, Brazil. The morphological description was based on live observations, after protargol staining, and scanning electron microscopy. The novel species has a cylindrical body shape that is slightly contractile, 230–550 × 35–65 μm, a cytoplasm with many globular inclusions, one row of irregular cortical granules between each somatic kinety, approximately 40–62 somatic kineties, two globular macronuclei measuring 9–24 μm and one micronucleus of approximately 4–9 μm. A subapical oral cavity was approximately 20–80 × 9–25 μm, with an adoral zone on the left side of the buccal field, which was composed of 32–60 polykineties and a paroral at the right side that was composed of 40–57 short polykineties. The new genus is distinguished from other geleiids by a loop-shaped posterior end of the paroral ciliature, made up of two rows of short polykineties, and the oralization of the central superior kinety (K0i), forming a row of dikinetids that borders the adoral zone internally, followed by several rows of monokinetids. In the phylogenetic analyses, the novel species was recovered as the sister group of Parduczia orbis with full support values based on 18S rRNA gene sequences. This work also indicates some problems in the definitions of the Geleiidae and proposes a new diagnosis for this karyorelictid family.

scholarly journals Multiple Types of Novel Enteric Bopiviruses (Picornaviridae) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary

Viruses ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 66
Author(s):  
Zoltán László ◽  
Péter Pankovics ◽  
Gábor Reuter ◽  
Attila Cságola ◽  
Ádám Bálint ◽  
...  
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Interspecies Transmission ◽  
Background Information ◽  
Type Ii ◽  
Rt Pcr ◽  
Faecal Samples ◽  
The Family ◽  
Domestic Livestock

Most picornaviruses of the family Picornaviridae are relatively well known, but there are certain “neglected” genera like Bopivirus, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, three novel picornaviruses provisionally called ovipi-, gopi- and bopivirus/Hun (MW298057-MW298059) from enteric samples of asymptomatic ovine, caprine and bovine respectively, were determined using RT-PCR and dye-terminator sequencing techniques. These monophyletic viruses share the same type II-like IRES, NPGP-type 2A, similar genome layout (4-3-4) and cre-localisations. Culture attempts of the study viruses, using six different cell lines, yielded no evidence of viral growth in vitro. Genomic and phylogenetic analyses show that bopivirus/Hun of bovine belongs to the species Bopivirus A, while the closely related ovine-origin ovipi- and caprine-origin gopivirus could belong to a novel species “Bopivirus B” in the genus Bopivirus. Epidemiological investigation of N = 269 faecal samples of livestock (ovine, caprine, bovine, swine and rabbit) from different farms in Hungary showed that bopiviruses were most prevalent among <12-month-old ovine, caprine and bovine, but undetectable in swine and rabbit. VP1 capsid-based phylogenetic analyses revealed the presence of multiple lineages/genotypes, including closely related ovine/caprine strains, suggesting the possibility of ovine–caprine interspecies transmission of certain bopiviruses.

scholarly journals Karyotype Characterization of Nine Periwinkle Species (Gastropoda, Littorinidae)

Genes ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 517 ◽  
Author(s):  
Daniel García-Souto ◽  
Sandra Alonso-Rubido ◽  
Diana Costa ◽  
José Eirín-López ◽  
Emilio Rolán-Álvarez ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Chromosome Numbers ◽  
Gene Clusters ◽  
Chromosomal Mapping ◽  
Closely Related Species ◽  
Submetacentric Chromosome ◽  
The Family ◽  
Littorina Arcana

Periwinkles of the family Littorinidae (Children, 1834) are common members of seashore littoral communities worldwide. Although the family is composed of more than 200 species belonging to 18 genera, chromosome numbers have been described in only eleven of them. A molecular cytogenetic analysis of nine periwinkle species, the rough periwinkles Littorina arcana, L. saxatilis, and L. compressa, the flat periwinkles L. obtusata and L. fabalis, the common periwinkle L. littorea, the mangrove periwinkle Littoraria angulifera, the beaded periwinkle Cenchritis muricatus, and the small periwinkle Melarhaphe neritoides was performed. All species showed diploid chromosome numbers of 2n = 34, and karyotypes were mostly composed of metacentric and submetacentric chromosome pairs. None of the periwinkle species showed chromosomal differences between male and female specimens. The chromosomal mapping of major and minor rDNA and H3 histone gene clusters by fluorescent in situ hybridization demonstrated that the patterns of distribution of these DNA sequences were conserved among closely related species and differed among less related ones. All signals occupied separated loci on different chromosome pairs without any evidence of co-localization in any of the species.

scholarly journals The genus Microniphargus (Crustacea, Amphipoda): evidence for three lineages distributed across northwestern Europe and transfer from Niphargidae to Pseudoniphargidae

2021 ◽  
Vol 151 ◽  
Author(s):  
Dieter Weber ◽  
Fabio Stoch ◽  
Lee R.F.D. Knight ◽  
Claire Chauveau ◽  
Jean-François Flot
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Analyses ◽  
Ribosomal Gene ◽  
Phylogenetic Position ◽  
Mitochondrial Cytochrome ◽  
Monotypic Genus ◽  
Centre Of Origin ◽  
Phylogenetic Studies ◽  
The Family

Microniphargus leruthi Schellenberg, 1934 (Amphipoda: Niphargidae) was first described based on samples collected in Belgium and placed in a monotypic genus within the family Niphargidae. However, some details of its morphology as well as recent phylogenetic studies suggest that Microniphargus may be more closely related to Pseudoniphargus (Amphipoda: Pseudoniphargidae) than to Niphargus. Moreover, M. leruthi ranges over 1,469 km from Ireland to Germany, which is striking since only a few niphargids have confirmed ranges in excess of 200 km. To find out the phylogenetic position of M. leruthi and check whether it may be a complex of cryptic species, we collected material from Ireland, England and Belgium then sequenced fragments of the mitochondrial cytochrome c oxidase subunit 1 gene as well as of the nuclear 28S ribosomal gene. Phylogenetic analyses of both markers confirm that Microniphargus is closer to Pseudoniphargus than to Niphargus, leading us to reallocate Microniphargus to Pseudoniphargidae. We also identify three congruent mito-nuclear lineages present respectively in Ireland, in both Belgium and England, and in England only (with the latter found in sympatry at one location), suggesting that M. leruthi is a complex of at least three species with a putative centre of origin in England.

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