Integrative description of five Pseudechiniscus species (Heterotardigrada: Echiniscidae: the suillus-facettalis complex)

Zootaxa ◽  
2020 ◽  
Vol 4763 (4) ◽  
pp. 451-484 ◽  
Author(s):  
MILENA ROSZKOWSKA ◽  
DARIA GROBYS ◽  
TOMASZ BARTYLAK ◽  
MAGDALENA GAWLAK ◽  
HANNA KMITA ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Tree ◽  
Genetic Data ◽  
Integrative Taxonomy ◽  
Genetic Distances ◽  
Morphological Features ◽  
Species Discrimination ◽  
Coi Sequences ◽  
Morphology And Morphometry ◽  
Species Specific

Pseudechiniscus is a morphologically homogeneous heterotardigrade genus with a relatively low number of morphological features useful for the species discrimination. The species of the Pseudechiniscus suillus-facettalis complex are some of the most challenging tardigrades to identify. Here, we examine several populations from Antarctica, Italy, Madagascar and Norway that would have most likely been attributed to Pse. suillus prior to the recent redescription of the species. Populations were analysed using integrative taxonomy—a combination of classical morphology and morphometry, as well as genetic data. Besides minute differences in dorsal sculpture and morphometry, we found clear, species-specific differences in ventral sculpture which are very useful in discrimination of Pseudechiniscus species. Based on morphology (mainly ventral sculpture) and significant genetic distances in COI and ITS-2 sequences, we describe five new species: Pse. angelusalas sp. nov. from Madagascar, Pse. dastychi sp. nov. from Antarctica, Pse. ehrenbergi sp. nov. from Italy as well as Pse. indistinctus sp. nov. and Pse. lacyformis sp. nov. from Norway. Finally, we provide an updated phylogenetic tree of the genus Pseudechiniscus based on COI sequences. 

High diversity in the Pseudechiniscus suillus–facettalis complex (Heterotardigrada: Echiniscidae) with remarks on the morphology of the genus Pseudechiniscus

2020 ◽  
Vol 188 (3) ◽  
pp. 733-752 ◽  
Author(s):  
Daria Grobys ◽  
Milena Roszkowska ◽  
Magdalena Gawlak ◽  
Hanna Kmita ◽  
Andrzej Kepel ◽  
...  
Keyword(s):  
Molecular Data ◽  
Integrative Taxonomy ◽  
Morphological Features ◽  
Species Discrimination ◽  
Extensive Discussion ◽  
Morphology And Morphometry ◽  
Species Specific ◽  
High Diversity ◽  
General Appearance

Abstract Pseudechiniscus is a morphologically homogeneous genus of tardigrades. The morphological features commonly used for species discrimination in this genus are the dorsal sculpture, the shape and number of dorsal plates and trunk appendages. Species of the Pseudechiniscus suillus–facettalis complex are one of the most challenging tardigrades to identify. All species are similar in their general appearance and all lack trunk appendages. Moreover, not only the nominal Pseudechiniscus suillus, but also other members of the suillus–facettalis complex have been insufficiently described. In our study, we examined several populations from the Northern and the Southern Hemispheres that could be traditionally attributed to Pse. suillus. These populations were analysed using integrative taxonomy – a combination of classical morphology and morphometry with molecular data. Besides the differences in the dorsal sculpture and morphometry, we also found species-specific differences in ventral sculpture, which were originally used for discrimination of Pseudechiniscus species. Moreover, we provide an extensive discussion on all morphological and morphometric differences used in Pseudechiniscus taxonomy and indicate main taxonomic problems with this genus. Finally, we redescribe the nominal Pse. suillus from Italy.

Taxonomy of Anterastes and related genera: a new synonym and a new species of Anterastes

Zootaxa ◽  
2011 ◽  
Vol 2771 (1) ◽  
pp. 41 ◽  
Author(s):  
SARP KAYA ◽  
BATTAL CIPLAK
Keyword(s):  
New Species ◽  
Phylogenetic Tree ◽  
Phylogenetic Trees ◽  
Genetic Data ◽  
New Combination ◽  
New Synonym ◽  
Male Calling ◽  
Phylogenetic Hypotheses ◽  
Distinct Features ◽  
A New Species

Among the Anatolian Tettigoniinae (Orthoptera, Tettigoniidae) the genera Anterastes, Koroglus, Sureyaella and Rhacocleis are distinguishable from the others by presence of one pair of spurs on the apico-ventral end of hind tibiae. The last two can be easily distinguished from the others by several distinct features, but the separation of the first two from each other is problematic. A new species described here provided opportunity of re-evaluating their taxonomy. The new species Anterastes antecessor sp. n. is described based on morphology, male calling song and genetic data. The taxonomy of Anterastes and Koroglus is rectified based on phylogentic hypotheses obtained from representative 16S rDNA haplotypes. Sureyaella bella, Parapholidoptera signata and Bolua turkiyae are used as out groups in different combinations to obtain a more stable phylogeny. Although analyses with different outgroups suggested the same topology, the phylogenetic tree with outgroups Parapholidoptera signata and Bolua turkiyae resulted with the highest bootstrap supports to the branches. Phylogenetic trees suggested the following relationships for the ingroup species; (A. antecessor sp. n. + ((Koroglus disparalatus + A. uludaghensis) + (A. turcicus + (A. niger + (A. ucari + A. babadaghi))) + ((A. tolunayi + (A. serbicus + A. antitauricus + A. burri)))). Considering the phylogenetic hypotheses and characters used in previous publications, Koroglus is put in synonymy with Anterastes, and a new combination is suggested for the only species of the former Anterastes disparalatus comb. n. A short remark is given about the characters used in the generic taxonomy of the group.

scholarly journals Four new species of the trapdoor spider genus Conothele Thorell, 1878 (Araneae, Halonoproctidae) from China

ZooKeys ◽  
2019 ◽  
Vol 833 ◽  
pp. 133-150
Author(s):  
Hao Liu ◽  
Xin Xu ◽  
Zengtao Zhang ◽  
Fengxiang Liu ◽  
Daiqin Li
Keyword(s):  
New Species ◽  
Nucleotide Substitution ◽  
Mitochondrial Gene ◽  
Genetic Distances ◽  
Jiangxi Province ◽  
Hainan Province ◽  
Genital Morphology ◽  
Species Specific ◽  
Specific Nucleotide ◽  
Female Genital

Herein four species of the trapdoor spider genus Conothele Thorell, 1878 collected from China are described as new to science based on the female genital morphology: C.baishasp. n. (Hainan Province), C.baotingsp. n. (Hainan Province), C.linzhisp. n. (Tibet), and C.jinggangshansp. n. (Jiangxi Province). For two Hainan species, C.baishasp. n. and C.baotingsp. n., between which it is difficult to distinguish solely based on female genital morphology, additional diagnoses derived from species-specific nucleotide substitution information and genetic distances using the mitochondrial gene, cytochrome c oxidase subunit I are provided.

scholarly journals The Complete Mitochondrial Genome of Bactrocera carambolae (Diptera: Tephritidae): Genome Description and Phylogenetic Implications

Insects ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 429
Author(s):  
Elena Drosopoulou ◽  
Alexandros Syllas ◽  
Panagiota Goutakoli ◽  
Georgios-Alkis Zisiadis ◽  
Theodora Konstantinou ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Integrative Taxonomy ◽  
Bactrocera Dorsalis ◽  
Species Discrimination ◽  
Bactrocera Carambolae ◽  
Phylogenetic Implications ◽  
Mitochondrial Sequences ◽  
Species Specific ◽  
Population Suppression

Bactrocera carambolae is one of the approximately 100 sibling species of the Bactrocera dorsalis complex and considered to be very closely related to B. dorsalis. Due to their high morphological similarity and overlapping distribution, as well as to their economic impact and quarantine status, the development of reliable markers for species delimitation between the two taxa is of great importance. Here we present the complete mitochondrial genome of B. carambolae sourced from its native range in Malaysia and its invaded territory in Suriname. The mitogenome of B. carambolae presents the typical organization of an insect mitochondrion. Comparisons of the analyzed B. carambolae sequences to all available complete mitochondrial sequences of B. dorsalis revealed several species-specific polymorphic sites. Phylogenetic analysis based on Bactrocera mitogenomes supports that B. carambolae is a differentiated taxon though closely related to B. dorsalis. The present complete mitochondrial sequences of B. carambolae could be used, in the frame of Integrative Taxonomy, for species discrimination and resolution of the phylogenetic relationships within this taxonomically challenging complex, which would facilitate the application of species-specific population suppression strategies, such as the sterile insect technique.

scholarly journals Integrative taxonomy ofMetrichiaRoss (Trichoptera: Hydroptilidae: Ochrotrichiinae) microcaddisflies from Brazil: descriptions of twenty new species

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2009 ◽  
Author(s):  
Allan P.M. Santos ◽  
Daniela M. Takiya ◽  
Jorge L. Nessimian
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
First Record ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Mato Grosso ◽  
Intraspecific Divergence ◽  
Coi Sequences

Metrichiais assigned to the Ochrotrichiinae, a group of almost exclusively Neotropical microcaddisflies.Metrichiacomprises over 100 described species and, despite its diversity, only one species has been described from Brazil so far. In this paper, we provide descriptions for 20 new species from 8 Brazilian states:M. acuminatasp. nov.,M. azulsp. nov.,M. bonitasp. nov.,M. bracuisp. nov.,M. caracasp. nov.,M. circuliformesp. nov.,M. curtasp. nov.,M. farofasp. nov.,M. forcepssp. nov.,M. formosinhasp. nov.,M. goianasp. nov.,M. itabaianasp. nov.,M. longissimasp. nov.,M. peludasp. nov.,M. rafaelisp. nov.,M. simplessp. nov.,M. talhadasp. nov.,M. teresp. nov.,M. ubajarasp. nov., andM. vulgarissp. nov.DNA barcode sequences (577 bp of the mitochondrial gene COI) were generated for 13 of the new species and two previously known species ofMetrichiaresulting in 64 sequences. In addition, COI sequences were obtained for other genera of Ochrotrichiinae (Angrisanoia,Nothotrichia,Ochrotrichia,Ragatrichia, andRhyacopsyche). DNA sequences and morphological data were integrated to evaluate species delimitations. K2P pairwise distances were calculated to generate a neighbor-joining tree. COI sequences also were submitted to ABGD and GMYC methods to assess ‘potential species’ delimitation. Analyses showed a conspicuous barcoding gap amongMetrichiasequences (highest intraspecific divergence: 4.8%; lowest interspecific divergence: 12.6%). Molecular analyses also allowed the association of larvae and adults ofMetrichia bonitasp. nov.from Mato Grosso do Sul, representing the first record of microcaddisfly larvae occurring in calcareous tufa (or travertine). ABGD results agreed with the morphological delimitation ofMetrichiaspecies, while GMYC estimated a slightly higher number of species, suggesting the division of two morphological species, each one into two potential species. Because this could be due to unbalanced sampling and the lack of morphological diagnostic characters, we have maintained these two species as undivided.

scholarly journals The first molecular insight into the genus Turanium Baeckmann, 1922 (Coleoptera: Cerambycidae: Callidiini) with a description of a new species from Middle Asia

2021 ◽  
Vol 79 ◽  
pp. 465-484
Author(s):  
Lech Karpiński ◽  
Wojciech T. Szczepański ◽  
Radosław Plewa ◽  
Lech Kruszelnicki ◽  
Katarzyna Koszela ◽  
...  
Keyword(s):  
New Species ◽  
Strong Support ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Central Asian ◽  
Ecological Associations ◽  
Coi Sequences ◽  
A New Species ◽  
Insight Into ◽  
Key Characters

This paper sheds the first light on the phylogeny of the Central Asian genus Turanium Baeckmann, 1922. By applying an integrative taxonomy approach, we revealed and described a new species from Kyrgyzstan—Turanium losi Karpiński, Plewa & Hilszczański sp. nov. Distinguishing characters from closely related Turanium pilosum (Reitter, 1891) are presented and their ecological associations are discussed. The key characters, including the male terminalia, were examined by means of scanning electron microscopy. High-quality stacked photographs of the habitus of the specimens are presented for both species and their geographical distributions are mapped. While the new species shows stable morphological characters that allow its differentiation from T. pilosum and the COI genetic distance between them is approx. 3%, the different species delimitation methods gave discordant results. Although the new species remained unrecognized for so long, it seems that these cerambycids are common in the region and both can be considered potentially invasive as they are apparently highly polyphagous. It has also been documented that they occur sympatrically in Kyrgyzstan. Both the Bayesian and maximum likelihood analyses of COI sequences confirmed the monophyly of the genus Turanium with strong support (PP 1 and BS 90, respectively). Moreover, the recently revealed polyphyly of the tribe Callidiini was supported by our analyses and, consequently, the discussion on the establishment of a new tribe Ropalopini is raised. This study further corroborates the effectiveness of DNA barcoding as a tool in detecting new species and provides some of the first sequences for Central Asian cerambycids, which remain almost completely unknown in terms of molecular studies.

Integrative taxonomy of Hemimycale (Hymedesmiidae: Poecilosclerida: Demospongiae) from Southeastern Brazil, with the description of two new species

Zootaxa ◽  
2018 ◽  
Vol 4442 (1) ◽  
pp. 137-152
Author(s):  
LARISSA HUGUENIN ◽  
SULA SALANI ◽  
MAIRA F. LOPES ◽  
RODOLPHO M. ALBANO ◽  
EDUARDO HAJDU ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Southeastern Brazil ◽  
Morphological Identification ◽  
Two New Species ◽  
Anatomical Characters ◽  
Coi Sequences ◽  
Morphological And Molecular Data ◽  
Reddish Orange

Two new species of Hemimycale from Southeastern Brazil are described in this study with the use of morphological and molecular data. Hemimycale oxeata sp. nov. is orange salmon colored in life and has unique oxeote like tornote spicules in addition to the typical (subtylo-)strongyles of the genus. Hemimycale ceadensis sp. nov. is reddish orange in life and has raised, tiny pore sieves, and subtylostrongyles with asymmetrical ends and raphides as microscleres. The diagnosis of the genus Hemimycale was slightly modified for the inclusion of the new species. Maximum-Likelihood analyses of 18S rRNA and COI sequences resulted in the clustering of both new species with the type species of the genus, thus confirming our morphological identification. The value of anatomical characters of pore sieves for diagnosing species and the genus is discussed. A key for species identification is also furnished. 

scholarly journals Diagnoses of two new species of Parosphromenus (Teleostei: Osphronemidae) from Bangka Island and Kalimantan, Indonesia

Zootaxa ◽  
2021 ◽  
Vol 5060 (1) ◽  
pp. 71-92
Author(s):  
WENTIAN SHI ◽  
SHUJIE GUO ◽  
HARYONO HARYONO ◽  
YIJIANG HONG ◽  
WANCHANG ZHANG
Keyword(s):  
New Species ◽  
Phylogenetic Tree ◽  
Cytochrome B ◽  
River System ◽  
Genetic Distances ◽  
The Body ◽  
Cytb Gene ◽  
Caudal Fin ◽  
Red Color ◽  
Two New Species

We describe two new species of Parosphromenus from Indonesia based on morphological and molecular diagnoses. Parosphromenus juelinae, sp. nov., occurs on Bangka Island. Its unpaired fin coloration is similar to that of P. deissneri, but it differs from the latter in having a rounded caudal fin with a non-filamentous branched median ray and a smaller anal fin. Although the new species has the same caudal fin structure as P. bintan, it can be distinguished from the latter by its distinct unpaired fin coloration and the intense red color on the body flanks. Parosphromenus kishii, sp. nov., is found only in a single river system in Kalimantan Tengah. It is distinguished from all other congeners by the unique coloration of its caudal fin. A phylogenetic tree based on the cytochrome b (cytb) gene indicates that the two new species are distinct monophyletic groups constituting distinct phylogenetic branches from their congeners. Cytochrome b Genetic distances between Parosphromenus juelinae, sp. nov., and Parosphromenus kishii, sp. nov., and the other taxa in the phylogenetic tree range from 2.44% to 19.52% and from 8.65% to 17.28%, respectively.  

scholarly journals A new species and first record of the genus Procerobaetis Kaltenbach & Gattolliat, 2020 (Ephemeroptera, Baetidae) from Thailand

ZooKeys ◽  
2021 ◽  
Vol 1023 ◽  
pp. 13-28
Author(s):  
Chanaporn Suttinun ◽  
Thomas Kaltenbach ◽  
Jean-Luc Gattolliat ◽  
Boonsatien Boonsoong
Keyword(s):  
New Species ◽  
Posterior Margin ◽  
First Record ◽  
Morphological Characters ◽  
Genetic Distances ◽  
The Other ◽  
Closely Related Species ◽  
Coi Sequences ◽  
First Time ◽  
A New Species

The genus Procerobaetis Kaltenbach & Gattolliat, 2020 is reported for the first time from Thailand, and Procerobaetis totuspinosussp. nov. is described as a new species based on larvae. It can be easily distinguished from other known Procerobaetis species by the presence of triangular spines at the posterior margin of tergites VI–IX. COI sequences were obtained from all known species. The genetic distances (Kimura 2-parameter) between the new species and the other species are between 20% and 23%. The morphological characters of the new species and its closely related species are discussed; larval key to all species of the genus Procerobaetis is also provided.

Molecular phylogenetic analysis and comparative morphology resolve two new species of olive-tree soil related dagger nematodes of the genus Xiphinema (Dorylaimida : Longidoridae) from Spain

2016 ◽  
Vol 30 (6) ◽  
pp. 547 ◽  
Author(s):  
Antonio Archidona-Yuste ◽  
Juan A. Navas-Cortés ◽  
Carolina Cantalapiedra-Navarrete ◽  
Juan E. Palomares-Rius ◽  
Pablo Castillo
Keyword(s):  
New Species ◽  
Iberian Peninsula ◽  
Olive Tree ◽  
Comparative Morphology ◽  
Integrative Taxonomy ◽  
Species Discrimination ◽  
Root Cells ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Olive Trees

The genus Xiphinema constitutes a large group of ~275 species of polyphagous, plant-ectoparasitic nematodes that are distributed almost worldwide. Some species affect agricultural crops by feeding directly on root cells as well as by transmitting nepoviruses. Despite their agricultural importance, species discrimination in Xiphinema is difficult, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses and morphology, constitutes a new insight into Xiphinema species identification. In this study we describe two new species of Xiphinema from the Iberian Peninsula (X. macrodora, sp. nov. and X. oleae, sp. nov.) associated with cultivated and wild olive trees. Both species have specific rRNA sequences. Morphologically, Xiphinema macrodora, sp. nov. is characterised by a very long body (7.2–8.7 mm), a very long odontostyle and odontophore (190–206 and 105–120 µm, respectively), and a well developed pseudo-Z-organ, comprising 8 to 12 sclerotised bodies. Xiphinema oleae, sp. nov. is characterised by an odontostyle and an odontophore 136–149 and 65–80 µm long, respectively, and a well developed Z-organ with refractive inclusions (3–5), variable in shape. Additionally, X. macrodora, sp. nov. has the longest body size, and the longest odontostyle and odontophore of any Xiphinema, whereas X. oleae, sp. nov. is the first species with a well developed Z-organ from the Iberian Peninsula. http://zoobank.org/urn:lsid:zoobank.org:pub:E4CE8711-22EF-40E4-9D39-6FE962F25225

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