First record of Branchipodopsis affinis Sars, 1901 (Crustacea: Anostraca) in Iran (Bazargan, West Azerbaijan): Ecology, Morphology and Genetics

We report the first record of Branchipodopsis affinis Sars, 1901 (Branchiopoda, Anostraca) from Iran. The specimens were collected in small temporary pools in the Bazargan area located in West Azerbaijan province, in spring 2015. Details on the biogeography, ecology and morphology of this species are provided. The DNA sequence data (COI) for this species is reported for the first time, which can be used in the identification of species on molecular terms and generation of information regarding the evolutionary relationship of the species in future. Also, the new finding is an important contribution to the knowledge of the anostracan fauna from Iran.

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Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

Helminthologia ◽

10.2478/s11687-007-0002-z ◽

2007 ◽

Vol 44 (2) ◽

pp. 43-46 ◽

Cited By ~ 5

Author(s):

D. Kuznetsov ◽

N. Kuznetsova

Keyword(s):

Dna Sequence ◽

Intraspecific Variation ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Moscow Region ◽

Dna Sequence Data ◽

Interspecific Differences ◽

Second Internal Transcribed Spacer ◽

First Time

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

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Immature stages, host plants and natural enemies of Henosepilachna implicata (Mulsant) (Coleoptera: Coccinellidae) with DNA sequence data and a new synonym and notes on some Indian species of Epilachnini

Zootaxa ◽

10.11646/zootaxa.4970.3.5 ◽

2021 ◽

Vol 4970 (3) ◽

pp. 533-546

Author(s):

J. POORANI ◽

R. G. BOOTH ◽

R. GANDHI GRACY ◽

C. ANURADHA ◽

R. THANIGAIRAJ ◽

...

Keyword(s):

Dna Sequence ◽

Natural Enemies ◽

Host Plants ◽

Sequence Data ◽

Indian Species ◽

Life Stages ◽

Important Species ◽

Dna Sequence Data ◽

New Synonym ◽

First Time

Life stages of Henosepilachna implicata (Mulsant), an economically important species of Epilachnini in India, are documented and illustrated. Mitochondrial DNA sequence data is provided for the first time for H. implicata with additional details on its host plants, distribution, and natural enemies. Its similarities and differences with other common pestiferous Henosepilachna spp. in India such as H. vigintioctopunctata (F.), H. septima (Dieke) and H. pusillanima (Mulsant) are discussed. Epilachna circularis Korschefsky, 1933 is found to be conspecific with H. implicata and is reduced to a junior synonym of the latter (new synonym). Notes are given on the distribution and natural enemies of some other species of Epilachnini of the Indian region.

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The Genera of Fungi – G6: Arthrographis, Kramasamuha, Melnikomyces, Thysanorea, and Verruconis

Fungal Systematics and Evolution ◽

10.3114/fuse.2020.06.01 ◽

2020 ◽

Vol 6 (1) ◽

pp. 1-24

Author(s):

M. Hernández-Restrepo ◽

A. Giraldo ◽

R. van Doorn ◽

M.J. Wingfield ◽

J.Z. Groenewald ◽

...

Keyword(s):

New Species ◽

Dna Sequence ◽

Type Species ◽

New Genus ◽

Sequence Data ◽

Dna Sequence Data ◽

Two New Species ◽

First Time

The Genera of Fungi series, of which this is the sixth contribution, links type species of fungal genera to their morphology and DNA sequence data. Five genera of microfungi are treated in this study, with new species introduced in Arthrographis, Melnikomyces, and Verruconis. The genus Thysanorea is emended and two new species and nine combinations are proposed. Kramasamuha sibika, the type species of the genus, is provided with DNA sequence data for first time and shown to be a member of Helminthosphaeriaceae (Sordariomycetes). Aureoconidiella is introduced as a new genus representing a new lineage in the Dothideomycetes.

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Description of the immature stages of the Neotropical whirligig beetle Gyrinus (Neogyrinus) rozei Ochs, 1953 (Coleoptera: Gyrinidae) and first report of the parasitoid wasp Melanosmicra sp. (Hymenoptera: Chalcididae) on a Gyrinus species

Zootaxa ◽

10.11646/zootaxa.4732.1.4 ◽

2020 ◽

Vol 4732 (1) ◽

pp. 99-116

Author(s):

DANIARA COLPANI ◽

CÉSAR JOÃO BENETTI ◽

NEUSA HAMADA ◽

VANDERLY ANDRADE-SOUZA ◽

KARINE SCHOENINGER ◽

...

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Parasitoid Wasp ◽

First Record ◽

The Body ◽

Immature Stages ◽

Cytochrome Oxidase Subunit ◽

Dna Sequence Data ◽

Gene Coi ◽

Northern Brazil

In most species of Gyrinidae, the immature stages are unknown, especially due to the difficulty in collecting the juveniles and assigning them to a particular species. Molecular association is a feasible technique that may solve this problem. Recent studies have used DNA sequence data, specifically the gene cytochrome oxidase subunit I (COI), to associate immature and adult stages, thus enabling the description of the former. The objectives of this study were (1) to describe and illustrate the immature stages of Gyrinus (Neogyrinus) rozei Ochs, 1953 including morphometric, chaetotaxic and bionomic information, and (2) to assess the usefulness of the gene COI to associate immatures and adults of Gyrinus. The studied specimens were collected in Roraima state, northern Brazil. The association of immature and adult stages was done either by rearing adults under laboratory conditions or by using DNA sequence data (COI). Eggs were described based on scanning electron microscopy; they are distinguished mainly by several features of chorion, micropyle and reticulation. Larvae of G. (N.) rozei can be distinguished from those of other Neotropical Gyrinidae by a combination of several characters, including the stipes with five robust hook-like additional setae on the dorsointernal margin, and the lacinia roughly hook-shaped. The pupa is similar to that of G. argentinus Steinhel, 1869, except for the number of setae on the body. The first record of the parasitoid wasp Melanosmicra sp. (Hymenoptera: Chalcididae) on a Gyrinus species is also provided.

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Molecular phylogenetics of the Onobrychis genus (Fabaceae: Papilionoideae) using ITS and trnL–trnF DNA sequence data

Australian Journal of Botany ◽

10.1071/bt13279 ◽

2014 ◽

Vol 62 (3) ◽

pp. 235 ◽

Cited By ~ 2

Author(s):

S. Safaei Chaei Kar ◽

F. Ghanavati ◽

M. R. Naghavi ◽

H. Amirabadi-zade ◽

R. Rabiee

Keyword(s):

Dna Sequence ◽

Molecular Phylogenetics ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Dna Sequence Data ◽

Group I ◽

Molecular Phylogenetic ◽

Nuclear Its ◽

Cultivated Species ◽

Relationship Of

Onobrychis, comprising more than 130 species, is a genus of the family Fabaceae. At this time, the interspecies relationship of this biologically important genus is still a subject of great discussion and debate. To help resolve this disagreement, we used molecular phylogeny to analyse internal transcribed spacer (ITS) and trnL–trnF sequences of 76 species of Onobrychis. Bayesian interference, maximum parsimony and maximum likelihood analyses of nuclear ITS and plastid trnL–trnF DNA sequence data generated trees with strong posterior probability for two groups: Onobrychis subgen. Sisyrosema (including: Heliobrychis, Hymenobrychis, Afghanicae and Anthyllium sections) along with Laxiflorae section in Group I and Onobrychis subgen. Onobrychis (except Laxiflorae section) in the other (Group II). The Laxiflorae section roots back to the ancestral node for Sisyrosema subgen. O. viciifolia (cultivated species), which is closely associated with O. cyri var. cyri, suggesting that the latter may be a wild progenitor of O. viciifolia. The present study supported the paraphyly of subgenera Onobrychis and Sisyrosema. The study proposed the paraphyletic nature of the sections Onobrychis, Dendrobrychis, Heliobrychis and Hymenobrychis. Together with our molecular phylogenetic analyses we present a review of Onobrychis morphology and discuss and compare our results with those of earlier morphological and molecular phylogenetic analyses.

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The challenge of integrative taxonomy of rare, deep-water gastropods: the genus Exilia (Neogastropoda: Turbinelloidea: Ptychatractidae)

Journal of Molluscan Studies ◽

10.1093/mollus/eyz037 ◽

2020 ◽

Vol 86 (2) ◽

pp. 120-138

Author(s):

Yuri I Kantor ◽

Nicolas Puillandre ◽

Philippe Bouchet

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Taxonomic Revision ◽

Distinct Species ◽

First Record ◽

Integrative Taxonomy ◽

Molecular Systematic ◽

Dna Sequence Data ◽

In The Wild ◽

Anatomical Characters

Abstract According to a recent taxonomic revision by Kantor et al. (2001), the neogastropod genus Exilia Conrad, 1860, comprises ten mostly rare species that live at depths between 200 and 2000 m. Adult Exilia measure between 30 and 90 mm in shell length, and the genus is mostly represented in museum collections by empty shells. The abundance of this genus is low in the wild, but recent expeditions organized by the Muséum national d’Histoire naturelle have yielded several dozen specimens. These new collections include samples preserved for molecular studies. Here, we present the results of the first molecular systematic study of Exilia. Our aim was to investigate the species limits proposed by Kantor et al. (2001) on the basis of shell and anatomical characters. Analysis of DNA sequence data for the cytochrome c oxidase I gene suggests that Exilia hilgendorfi, previously considered to be a single, polymorphic and broadly distributed species, is a complex of at least six species (four of which we sequenced). Two of these species, Exilia cognata n. sp. and E. fedosovi n. sp., are described as new to science. Exilia gracilior, E. claydoni and E. prellei are resurrected from the synonymy of Exilia hilgendorfi; of these three, only the last was sequenced. Exilia vagrans is a well-defined taxon, but our molecular systematic data shows that it consists of two distinct species, which occur sympatrically off Taiwan and are strikingly similar in shell and radular morphology; due to the absence of DNA sequence data from the type locality of E. vagrans (Vanuatu), it is unclear to which of these two species the name would apply. Exilia karukera n. sp., which is conchologically very similar to E. vagrans, was discovered off Guadeloupe, represents the first record of the genus from the Atlantic. For E. elegans, which was previously known only from a single shell, we provide new data including new distributional records (South Africa and the Mozambique Channel), details of the radula and DNA sequence data.

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Delayed germination of basidiospores in temperate species of Crepidotus (Fr.) Staude

Canadian Journal of Botany ◽

10.1139/b02-009 ◽

2002 ◽

Vol 80 (3) ◽

pp. 280-287 ◽

Cited By ~ 5

Author(s):

M C Aime ◽

O K Miller Jr.

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Abiotic Factors ◽

Large Subunit ◽

Early Spring ◽

Single Spore ◽

Dna Sequence Data ◽

Delayed Germination ◽

First Time ◽

Ribosomal Dna Sequence

Delayed basidiospore germination (endogenous dormancy) for a number of species of Crepidotus (Agaricales: Crepidotaceae) is reported for the first time. Ninety percent of recovered single spore isolates germinated between 18 and 36 weeks after collection; average germination was 25 weeks after collection. The period in which 90% of germinations occurred was between 17 February and 19 April, with 50% of recovered isolates germinating in March. Many abiotic factors were experimentally manipulated in an effort to reduce or alter the necessary incubation period without effect. The latent period was consistent for a given collection, with the majority of recovered isolates from fall-fruiting collections germinating during early spring, regardless of whether spores were plated immediately after harvesting or stored for one to several months prior to plating. The identity of the cultures derived from delayed germination was confirmed by DNA sequencing.Key words: basidiospore, dormancy, single spore isolates, Crepidotaceae, large subunit ribosomal DNA sequence data, phylogeny.

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Unravelling unexplored diversity of cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) in tropical Africa

MycoKeys ◽

10.3897/mycokeys.81.67850 ◽

2021 ◽

Vol 81 ◽

pp. 69-138

Author(s):

Yalemwork Meswaet ◽

Ralph Mangelsdorff ◽

Nourou S. Yorou ◽

Meike Piepenbring

Keyword(s):

West Africa ◽

Dna Sequence ◽

Vigna Unguiculata ◽

Sequence Data ◽

Morphological Characteristics ◽

Molecular Sequence Data ◽

Dna Sequence Data ◽

Wide Range ◽

Cercosporoid Fungi ◽

First Time

Cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) are one of the largest and most diverse groups of hyphomycetes causing a wide range of diseases of economically important plants as well as of plants in the wild. Although more than 6000 species are known for this group, the documentation of this fungal group is far from complete. Especially in the tropics, the diversity of cercosporoid fungi is poorly known. The present study aims to identify and characterise cercosporoid fungi collected on host plants belonging to Fabaceae in Benin, West Africa. Information on their morphology, host species and DNA sequence data (18S rDNA, 28S rDNA, ITS and tef1) is provided. DNA sequence data were obtained by a simple and non-culture-based method for DNA isolation which has been applied for cercosporoid fungi for the first time in the context of the present study. Among the loci used for the phylogenetic analysis, tef1 provided the best resolution together with the multigene dataset. Species delimitation in many cases, however, was only possible by combining molecular sequence data with morphological characteristics. Based on forty specimens recently collected in Benin, 18 species are presented with morphological descriptions, illustrations and sequence data. Among these, six species in the genus Cercospora and two species in Pseudocercospora are proposed as species new to science. The newly described species are Cercospora (C.) beninensis on Crotalaria macrocalyx, C. parakouensis on Desmodium tortuosum, C. rhynchophora on Vigna unguiculata, C. vignae-subterraneae on Vigna subterranea, C. tentaculifera on Vigna unguiculata, C. zorniicola on Zornia glochidiata, Pseudocercospora sennicola on Senna occidentalis and Pseudocercospora tabei on Vigna unguiculata. Eight species of cercosporoid fungi are reported for Benin for the first time, three of them, namely C. cf. canscorina, C. cf. fagopyri and C. phaseoli-lunati are new for West Africa. The presence of two species of cercosporoid fungi on Fabaceae previously reported from Benin, namely Nothopassalora personata and Passalora arachidicola, is confirmed.

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Ecology and seasonality of Pseudo-nitzschia species (Bacillariophyceae) in the northwestern Adriatic Sea over a 30-years period (1988–2020)

Mediterranean Marine Science ◽

10.12681/mms.26021 ◽

2021 ◽

Vol 22 (3) ◽

pp. 505

Author(s):

SONIA GIULIETTI ◽

TIZIANA ROMAGNOLI ◽

ALESSANDRA CAMPANELLI ◽

CECILIA TOTTI ◽

STEFANO ACCORONI

Keyword(s):

Dna Sequence ◽

Dna Sequences ◽

Species Complex ◽

Adriatic Sea ◽

Sequence Data ◽

Coastal Station ◽

Dna Sequence Data ◽

The Mean ◽

First Time ◽

North Western

The ecology and seasonality of Pseudo-nitzschia species and their contribution to phytoplankton community were analysed for the first time at the coastal station of the LTER-Senigallia-Susak transect (north-western Adriatic Sea) from 1988 to 2020. Species composition was addressed using DNA sequence data obtained from 106 monoclonal strains isolated from January 2018 to January 2020. The mean annual cycle of total phytoplankton in the study period (Feb 1988–Jan 2020) showed maximum abundances in winter followed by other peaks in spring and autumn. Diatoms were the main contributors in terms of abundance during the winter and the spring blooms. The autumn peak was due to phytoflagellates and diatoms. In summer phytoflagellates dominated the community, followed by diatoms and dinoflagellates, which in this season reached their annual maximum. Pseudo-nitzschia spp. represented on average 0.4–17.6% of diatom community, but during their blooms they could reach up to up to 90% of the total diatom abundances with 106 cells l-1. By LM, six different taxa were recognized: Pseudo-nitzschia cf. delicatissima and P. cf. pseudodelicatissima were the most abundant, followed by P. cf. fraudulenta, P. pungens, P. multistriata and P. cf. galaxiae. P. cf. fraudulenta and P. pungens were indicator taxa of winter. P. cf. delicatissima and P. cf. pseudodelicatissima were spring and summer taxa, respectively. P. galaxiae showed maximum abundances in autumn. DNA sequences revealed the presence of two species belonging to the ’P. seriata group’ (i.e. P. fraudulenta and P. pungens) and four species belonging to the ‘P. delicatissima group’ (P. calliantha and P. mannii within the P. pseudodelicatissima species complex, and P. delicatissima and P. cf. arenysensis within the P. delicatissima species complex). The presence of several cryptic and pseudo-cryptic species highlights the need to combine LM observations with DNA sequence data when the ecology of Pseudo-nitzschia is investigated.

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Nerve cells of Xenoturbella bocki (phylum uncertain) and Harrimania kupfferi (Enteropneusta) are positively immunoreactive to antibodies raised against echinoderm neuropeptides

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315405012725 ◽

2005 ◽

Vol 85 (6) ◽

pp. 1519-1524 ◽

Cited By ~ 19

Author(s):

Thomas Stach ◽

Samuel Dupont ◽

Olle Israelson ◽

Geraldine Fauville ◽

Hiroaki Nakano ◽

...

Keyword(s):

Phylogenetic Relationship ◽

Dna Sequence ◽

Sequence Data ◽

Nerve Cells ◽

Phylogenetic Position ◽

Dna Sequence Data ◽

Close Phylogenetic Relationship ◽

Positive Immunoreactivity ◽

Phylogenetic Hypotheses ◽

Relationship Of

The phylogenetic position of Xenoturbella spp. has been uncertain since their discovery in 1949. It has been recently suggested that they could be related to Ambulacraria within Deuterostomia. Ambulacraria is a taxon that has been suggested to consist of Hemichordata and Echinodermata. The hypothesis that X. bocki was related to Ambulacraria as well as the hypothesis of a monophyletic Ambulacraria is primarily based on the analysis of DNA sequence data. We tested both phylogenetic hypotheses using antibodies raised against SALMFamide 1 and 2 (S1, S2), neuropeptides isolated from echinoderms, on X. bocki and the enteropneust Harrimania kupfferi. Both species showed distinct positive immunoreactivity against S1 and S2. This finding supports the Ambulacraria-hypothesis and suggests a close phylogenetic relationship of X. bocki to Ambulacraria. In particular, the presence of immunoreactivity against S2 can be interpreted as a synapomorphy of Enteropneusta, Echinodermata, and Xenoturbella spp.

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