An integrative description of Diploechiniscus oihonnae (Richters, 1903) population from near the original type locality in Merok (Norway)

Zootaxa ◽  
2021 ◽  
Vol 4964 (1) ◽  
pp. 83-102
Author(s):  
ŁUKASZ KACZMAREK ◽  
PUSHPALATA KAYASTHA ◽  
MAGDALENA GAWLAK ◽  
MONIKA MIODUCHOWSKA ◽  
MILENA ROSZKOWSKA
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Morphological Variability ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Genetic Differences ◽  
Type Locality ◽  
28S Rrna ◽  
Life Stages ◽  
Original Type

In the present study, we used integrative taxonomy to describe a population of Diploechiniscus oihonnae (Richters, 1903) from the neotype locality in Merok (Norway). We found no differences in the chaetotaxy formula between the life stages and sex of Dpl. oihonnae. The presence of filaments at Bd in some specimens of Dpl. oihonnae appears to be random and most likely represents the morphological variability of this species. We also obtained DNA sequences of 18S rRNA, 28S rRNA, ITS-2, and COI of Dpl. oihonnae from the neotype locality for comparison with the sequences available in GenBank, which showed low genetic differences between the neotypic population and specimens from other localities. Therefore, we decided to establish our specimens from Merok as neotype and neoparatypes of Dpl. oihonnae. Additionally, based on morphological characters, Dpl. horningi (Schuster & Grigarick, 1971) was synonymised with Dpl. oihonnae.

scholarly journals New Records of Dactylobiotus parthenogeneticus Bertolani, 1982 Provide Insight into Its Genetic Variability and Geographic Distribution

2020 ◽  
Vol 68 (2) ◽  
pp. 57-72 ◽  
Author(s):  
Justyna Pogwizd ◽  
Daniel Stec
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Haplotype Diversity ◽  
Original Description ◽  
Integrative Taxonomy ◽  
Morphological Data ◽  
Morphological Identification ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
Insight Into

In sediment samples collected from three distinct European locations (United Kingdom, France, Poland), populations of Dactylobiotus parthenogeneticus were found. The original description of this species was based solely on the morphology observed with light microscopy and later supplemented by some additional SEM data of the buccal apparatus and DNA sequences of 18S rRNA and COI. Here we provide an updated description of the species by means of integrative taxonomy. The description comprises a comprehensive set of morphometric and morphological data from light and scanning microscopy as well as nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) fragments. Our analysis of haplotype diversity confirmed our morphological identification and showed that D. parthenogeneticus is widely distributed in Europe.

Variability of Echiniscus tristis Gąsiorek & Kristensen, 2018—is morphology sufficient for taxonomic differentiation of Echiniscidae?

Zootaxa ◽  
2019 ◽  
Vol 4701 (1) ◽  
pp. 1-24 ◽  
Author(s):  
TOMASZ BARTYLAK ◽  
ADAM KULPA ◽  
DARIA GROBYS ◽  
MARTA KEPEL ◽  
ANDRZEJ KEPEL ◽  
...  
Keyword(s):  
Dna Sequences ◽  
28S Rrna ◽  
Life Stages ◽  
Significant Genetic Differentiation ◽  
Wide Range ◽  
Microscope Images ◽  
Different Types ◽  
Taxonomic Confusion ◽  
Species Specific ◽  
Range Of Variation

The majority of species in the genus Echiniscus (Heterotardigrada) have been described based on differences in the chaetotaxy or dorsal sculpture. Dorsal sculpture is, in general, considered to be species-specific and not very variable; however, many problems have arisen due to various interpretations of microscope images, which has led to taxonomic confusion in the genus Echiniscus. Conversely, chaetotaxy is generally much easier to interpret, even using low-quality microscope optics. In this study, we emended the description of Madagascan population of Echiniscus tristis Gąsiorek & Kristensen, 2018 that exhibits several different types of chaetotaxy and dorsal sculpture. The analysed specimens were characterised by two types of chaetotaxy, A-C-Dd-E and A-Dd-E, but we also found a wide range of variation in appendage number, shape and length. The observed differences are partly correlated with life stages. Additionally, we analysed DNA sequences of 28S rRNA, ITS-2 and COI of the two main morphotypes, and did not find significant genetic differentiation of the two morphotypes. This highlights the importance of analysing the morphology of both immature stages and adults, as well as of DNA markers in tardigrade species identification. 

An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens

Zootaxa ◽  
2011 ◽  
Vol 3104 (1) ◽  
pp. 42 ◽  
Author(s):  
MICHELE CESARI ◽  
ILARIA GIOVANNINI ◽  
ROBERTO BERTOLANI ◽  
LORENA REBECCHI
Keyword(s):  
Dna Barcoding ◽  
Dna Barcode ◽  
Intraspecific Variability ◽  
Original Description ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Type Locality ◽  
Moss Sample ◽  
Voucher Specimens

We have in recent papers revealed that an integrative taxonomy approach helps to solve taxonomic problems in tardigrades. However, whole tardigrades are required for DNA work, which leaves no hologenophore voucher specimens with adult morphology. Using a novel methodology for the Tardigrada, we introduce the practice of collecting high quality maximum magnification light microscopy images of recently thawed animals to act as hologenophore voucher specimens of animals later used for DNA barcode sequencing. Within the framework of a DNA barcoding project on tardigrades, we collected a moss sample from the type locality of Macrobiotus terminalis Bertolani & Rebecchi, 1993 (Castelsantangelo, Central Apennines, Italy), a species of the “Macrobiotus hufelandi group”. Within the moss sample we found several animals and eggs with a morphology that corresponded to the original description of M. terminalis, while others were attributable to Macrobiotus macrocalix Bertolani & Rebecchi, 1993. In this study, molecular (cox1 mtDNA) analyses demonstrated no intraspecific variability in M. terminalis from the type locality but very large interspecific differences when compared with M. macrocalix and GenBank data for other species within the M. “hufelandi group”. There was also a large difference between our M. terminalis sequences and the GenBank data of a specimen attributed to the same species. The GenBank sequence originated from a population in the Northern Apennines, whose morphology appeared to be like that of the specimens of the locus typicus. This confirmed the importance in utilising material from the type locality for linking molecular data to the species’ morphological characters. Our paper underlines the importance of an integrative taxonomy in species diagnoses and demonstrates a scenario where morphological observations alone are not always sufficient. Lastly, this work adds reliable information to the sequence reference library that provides a useful building block for further studies on similar and related tardigrade taxa.

scholarly journals A new record of odd-scaled snake (Serpentes, Xenodermidae) from Vietnam: expanded description of Parafimbrios vietnamensis based on integrative taxonomy

ZooKeys ◽  
2021 ◽  
Vol 1048 ◽  
pp. 79-89
Author(s):  
Nikolai L. Orlov ◽  
Oleg A. Ermakov ◽  
Tao Thien Nguyen ◽  
Natalia B. Ananjeva
Keyword(s):  
Genetic Divergence ◽  
New Record ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Type Locality ◽  
Morphological Data ◽  
Snake Species ◽  
Male Individual

Based on the combination of molecular and morphological data, we herein report the second known finding of the xenodermid snake species Parafimbrios vietnamensis Ziegler, Ngo, Pham, Nguyen, Le & Nguyen, 2018. The male individual was found in the Yen Bai Province of northwestern Vietnam, more than 200 km from the type locality in Lai Chau Province. Genetic divergence between the newly-collected male and the holotype was low (1.7%), and is in agreement with morphological data that supports that they are conspecific. We give a detailed description of the morphological characters and coloration of the new record and provide an expanded diagnosis of P. vietnamensis. Parafimbrios is a poorly-understood genus, and our recent discovery brings the total number of known specimens of the genus to nine, 1/3 of them having been found in Vietnam (one specimen of P. lao and now two specimens of P. vietnamensis).

scholarly journals Integrative descriptions of two new Macrobiotus species (Tardigrada, Eutardigrada, Macrobiotidae) from Mississippi (USA) and Crete (Greece)

2021 ◽  
Vol 97 (1) ◽  
pp. 281-306
Author(s):  
Matteo Vecchi ◽  
Daniel Stec
Keyword(s):  
18S Rrna ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Distal Portion ◽  
Morphological Data ◽  
28S Rrna ◽  
Morphometric Characters ◽  
Clade B ◽  
Specific Morphology ◽  
Definition Of

In this paper, we describe two new Macrobiotus species from Mississippi (USA) and Crete (Greece) by means of integrative taxonomy. Detailed morphological data from light and scanning electron microscopy, as well as molecular data (sequences of four genetic markers: 18S rRNA, 28S rRNA, ITS-2 and COI), are provided in support of the descriptions of the new species. Macrobiotus annewintersaesp. nov. from Mississippi belongs to the Macrobiotus persimilis complex (Macrobiotus clade B) and exhibits a unique egg processes morphology, similar only to Macrobiotus anemone Meyer, Domingue & Hinton, 2014, but mainly differs from that species by the presence of eyes, granulation on all legs, dentate lunulae on legs IV, and of bubble-like structures within the tentacular arms that are present on the distal portion of the egg processes. Macrobiotus rybakisp. nov. from Crete belongs to the Macrobiotus clade A and is most similar to Macrobiotus dariae Pilato & Bertolani, 2004, Macrobiotus noemiae Roszkowska & Kaczmarek, 2019, Macrobiotus santoroi Pilato & D’Urso, 1976, and Macrobiotus serratus Bertolani, Guidi & Rebecchi, 1996, but differs from them mainly in the morphological details of its egg processes and chorion reticulation, but also by a number of morphometric characters. In light of the specific morphology of the egg processes of Macrobiotus annewintersaesp. nov. and Macrobiotus anemone, that are equipped with tentacular arms instead of proper terminal disc, we also provide an updated definition of the Macrobiotus persimilis complex.

scholarly journals AN INTEGRATIVE TAXONOMY OF MOLECULAR AND TRADITIONAL APPROACHES FOR IDENTIFICATION OF A STORAGE MITE, ALEUROGLYPHUS OVATUS (ACARI: ASTIGMATA: ACARIDAE) IN MALAYSIA

2016 ◽  
Vol 6 (2) ◽  
pp. 882-889
Author(s):  
Ernieenor Faraliana Che Lah
Keyword(s):  
Molecular Identification ◽  
Dna Sequences ◽  
Correct Diagnosis ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Its2 Region ◽  
Morphological Identification ◽  
Taxonomic Identification ◽  
Internal Transcribed Spacer Region ◽  
Accurate Identification

A reliable and rapid taxonomic identification of a mite is the basis for a correct diagnosis of important mite associated allergies as they produce species-specific allergens. A double approach (molecular and morphological) to the taxonomic identification of Aleuroglyphus ovatus was presented. Molecular identification was performed with amplification of the internal transcribed spacer region (ITS2), whilst morphological characters were examined under light microscope. The BLAST results obtained from molecular analysis of A. ovatus was shown to be in concordance with the morphological identification with 97% genetic similarity. Thus, the molecular identification based on the ITS2 region can be applied as a reliable and efficient tool for species identification of Aleuroglyphus and probably any other astigmatid mites. Our findings suggest the need for a broad taxonomic sampling especially from closely related species for an accurate identification of local mites using both DNA sequences and morphology.

scholarly journals Two new species of the Macrobiotus hufelandi complex (Tardigrada: Eutardigrada: Macrobiotidae) from Australia and India, with notes on their phylogenetic position

2019 ◽  
Author(s):  
Kyle Coughlan ◽  
Daniel Stec
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Taxonomic Status ◽  
Phylogenetic Position ◽  
28S Rrna ◽  
Future Studies ◽  
Mitochondrial Coi ◽  
Definition Of ◽  
Group Species ◽  
Independent Species

In this paper we describe two new tardigrade species belonging to the Macrobiotus hufelandi complex: Macrobiotus noongaris sp. nov. from Perth, Australia, and Macrobiotus kamilae sp. nov. from Mussoorie, India. Live specimens extracted from moss samples were used to establish laboratory cultures in order to obtain additional animals and eggs needed for their integrative descriptions. These descriptions are based on traditional morphological and morphometric data collected using both light and scanning electron microscopy, which, at the same time, were associated with DNA sequences of four genetic markers, three nuclear (18S rRNA, 28S rRNA and ITS-2) and one mitochondrial (COI). The use of DNA sequences allowed for a more accurate verification of the taxonomic status of M. noongaris sp. nov. and M. kamilae sp. nov as independent species of the hufelandi group. Although they both exhibit typical inverted goblet-shaped processes, they represent a recently discovered clade, which was thought to group species with modified morphology of egg processes. Thus, this contribution expands the definition of the mentioned clade and constitutes another link that will be helpful for future studies on the evolution of the M. hufelandi complex.

scholarly journals Integrative taxonomy of a new and highly-diverse genus of onchidiid slugs from the Coral Triangle (Gastropoda, Pulmonata, Onchidiidae)

ZooKeys ◽  
2018 ◽  
Vol 763 ◽  
pp. 1-111 ◽  
Author(s):  
Tricia C. Goulding ◽  
Munawar Khalil ◽  
Shau Hwai Tan ◽  
Benoît Dayrat
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Comparative Anatomy ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
The Philippines ◽  
Coral Triangle ◽  
Yellow Orange

A new genus of onchidiid slugs,WallaconchisGoulding & Dayrat,gen. n., is described, including ten species. Five species were previously described but known only from the type material:Wallaconchisater(Lesson, 1830),W.graniferum(Semper, 1880),W.nangkauriense(Plate, 1893),W.buetschlii(Stantschinsky, 1907), andW.gracile(Stantschinsky, 1907), all of which were originally classified inOnchidiumBuchannan, 1800. Many new records are provided for these five species, which greatly expand their known geographic distributions. Five species are new:WallaconchisachleitneriGoulding,sp. n.,W.comendadoriGoulding & Dayrat,sp. n.,W.melanesiensisGoulding & Dayrat,sp. n.,W.sinanuiGoulding & Dayrat,sp. n., andW.uncinusGoulding & Dayrat,sp. n.Nine of the tenWallaconchisspecies are found in the Coral Triangle (eastern Indonesia and the Philippines). Sympatry is high, with up to six species found on the island of Bohol (Philippines) and eight species overlapping in northern Sulawesi (Indonesia).Wallaconchisis distinguished from other onchidiids by its bright dorsal colors (red, yellow, orange) but those are extremely variable and not useful for specific identification. Internally, the reproductive system can be used to identify allWallaconchisspecies. The copulatory organs ofWallaconchisspecies are especially diverse compared to other onchidiid genera, and the possible role of reproductive incompatibility in species diversification is discussed. All specimens examined were freshly collected for the purpose of a worldwide revision of the Onchidiidae Rafinesque, 1815. The species are well delineated using DNA sequences and comparative anatomy. Mitochondrial DNA analysis yields thirteen molecular units separated by a large barcode gap, while nuclear DNA yields nine units. By integrating nuclear DNA and mitochondrial DNA with morphology, ten species are recognized. The natural history of each species (e.g., the microhabitat where they are found) is also documented. Nomenclature is addressed thoroughly (the types of all onchidiid species were examined, lectotypes were designated when needed,nomina dubiaare discussed). Morphological characters, transitions to new microhabitats, and diversification processes are discussed in the context of a robust molecular phylogeny.

A century later - a total evidence re-evaluation of the phylogeny of scutigeromorph centipedes (Myriapoda:Chilopoda)

2006 ◽  
Vol 20 (5) ◽  
pp. 503 ◽  
Author(s):  
Gregory D. Edgecombe ◽  
Gonzalo Giribet
Keyword(s):  
Molecular Markers ◽  
18S Rrna ◽  
Sequence Data ◽  
Histone H3 ◽  
Sister Group ◽  
Morphological Characters ◽  
Molecular Data ◽  
Total Evidence ◽  
28S Rrna ◽  
Group Relationships

Scutigeromorpha (‘house centipedes’) play a pivotal role in myriapod systematics in being the sister group to all other chilopods, but their internal phylogeny has not been comprehensively appraised since K. W. Verhoeff’s morphological investigations a century ago. Relationships between the three families of Scutigeromorpha are inferred based on a combined analysis of approximately 5.5 Kb of sequence data from five molecular markers (complete 18S rRNA, a 2.2-Kb fragment of 28S rRNA, 16S rRNA, cytochrome c oxidase subunit I, histone H3) and 33 ingroup morphological characters. Molecular data are available for 19 ingroup terminals representing 14 morphospecies that include the genera Scutigerina, Madagassophora (family Scutigerinidae), Sphendononema (family Pselliodidae), Scutigera, Thereuopoda, Thereuopodina, Thereuonema, Allothereua and Parascutigera (family Scutigeridae). Morphology resolves the southern African–Malagasy Scutigerinidae as sister to all other Scutigeromorpha, whereas rival sister-group relationships between the Neotropical–Afrotropical Pselliodidae and Scutigerinidae + Scutigeridae or Pselliodidae + Scutigerinidae and Scutigeridae are resolved by the molecular and combined analyses. Monophyly of Scutigeridae and Thereuoneminae are stable across a broad range of analytical parameters. Thereuoneminae is composed of two stable clades: an Allothereua + Parascutigera group, and a grouping of Thereuopoda, Thereuonema and Thereuopodina. Molecular and combined analyses resolve the genus Scutigerina and the morphospecies Scutigerina weberi as paraphyletic, in both cases with a Malagasy clade excluding populations from southern Africa.

scholarly journals Integrated taxonomy supports the identification of some species of Phytoseiidae (Acari: Mesostigmata) from Georgia

Acarologia ◽  
2021 ◽  
Vol 61 (4) ◽  
pp. 824-844
Author(s):  
Marie-Stephane Tixier ◽  
Philippe Auger ◽  
Alain Migeon ◽  
Martial Douin ◽  
Amandine Fossoud ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Abundant Species ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
12S Rrna ◽  
Amblyseius Andersoni ◽  
Integrated Taxonomy ◽  
Well Differentiated ◽  
Mtdna Markers ◽  
Eleven Species

The present study reports results of a survey carried out mostly on Citrus sp. and Rubus sp. in Georgia. Morphological and molecular (12S rRNA, COI and CytB mtDNA markers) data were analysed in a framework of integrative taxonomy. Eleven species were identified and among them seven are new for the Georgian fauna. Euseius stipulatus and Phytoseius finitimus were the most abundant species during this survey. We assume that Amblyseius eharai, only reported from eastern Asia, was most probably introduced. Neoseiulus californicus, retrieved from uncultivated vegetation, was almost certainly originating from commercial strains. DNA sequences comparisons disclosed phylogenetic closeness between Amblyseius andersoni and Transeius wainsteini, despite these species (i) being morphologically well differentiated and (ii) classified in different genera, thereby questioning the reliability of the genus Transeius. General morphological characters, including measurements, are provided for species for which diagnoses were doubtful.

Sign in / Sign up

Export Citation Format

Share Document