Regulator of Cell Cycle Protein (RGCC/RGC-32) Protects Against Pulmonary Fibrosis

2021 ◽  
Author(s):  
Irina G. Luzina ◽  
Violeta Rus ◽  
Virginia Lockatell ◽  
Jean-Paul Courneya ◽  
Brian S. Hampton ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Pulmonary Fibrosis ◽  
Cell Cycle Protein

scholarly journals Effects of Aspergillus fumigatus Conidia on Apoptosis and Proliferation in an In Vitro Model of the Lung Microenvironment

2021 ◽  
Vol 9 (7) ◽  
pp. 1435
Author(s):  
Hisako Kushima ◽  
Toshiyuki Tsunoda ◽  
Taichi Matsumoto ◽  
Yoshiaki Kinoshita ◽  
Koichi Izumikawa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Epithelial Cells ◽  
Pulmonary Fibrosis ◽  
Aspergillus Fumigatus ◽  
Rna Sequencing ◽  
A549 Cells ◽  
Western Blots ◽  
Mesenchymal Transition ◽  
Expression Of Genes ◽  
Nih 3T3

Background/Aim: Aspergillus is often detected in respiratory samples from patients with chronic respiratory diseases, including pulmonary fibrosis, suggesting that it can easily colonize the airways. To determine the role of Aspergillus colonization in pulmonary fibrosis, we cultured human lung epithelial A549 cells or murine embryo fibroblast NIH/3T3 cells with Aspergillus conidia in 3D floating culture representing the microenvironment. Materials and Methods: Cells were cultured in two-dimensional (2D) and three-dimensional floating (3DF) culture with heat-inactivated Aspergillus fumigatus (AF) 293 conidia at an effector-to-target cell ratio of 1:10 (early-phase model) and 1:100 (colonization model), and RNA-sequencing and Western blots (WB) were performed. Results: AF293 conidia reduced A549 cell growth in 2D and 3DF cultures and induced apoptosis in A549 spheroids in 3DF culture. RNA-sequencing revealed the increased expression of genes associated with interferon-mediated antiviral responses including MX dymamin-like GTPase 1 (MX1). Interestingly, the decreased expression of genes associated with the cell cycle was observed with a high concentration of AF293 conidia. WB revealed that epithelial-mesenchymal transition was not involved. Notably, AF293 conidia increased NIH/3T3 growth only in 3DF culture without inducing an apoptotic reaction. RNA-sequencing revealed the increased expression of genes associated with interferon signalling, including MX2; however, the decreased expression of genes associated with the cell cycle was not observed. Conclusions: AF affects both apoptosis of epithelial cells and the growth of fibroblasts. A deeper understanding of the detailed mechanisms underlying Aspergillus-mediated signaling pathway in epithelial cells and fibroblasts will help us to understand the lung microenvironment.

scholarly journals Cell-Cycle Protein Expression in a Population-Based Study of Ovarian and Endometrial Cancers

2015 ◽  
Vol 5 ◽  
Author(s):  
Ashley S. Felix ◽  
Mark E. Sherman ◽  
Stephen M. Hewitt ◽  
Munira Z. Gunja ◽  
Hannah P. Yang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Expression ◽  
Population Based ◽  
Cell Cycle Protein ◽  
Population Based Study ◽  
Endometrial Cancers

Effect of total saponins of “panax notoginseng root” on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats

Phytomedicine ◽  
2010 ◽  
Vol 17 (3-4) ◽  
pp. 233-240 ◽  
Author(s):  
Lu Wu ◽  
Wei Zhang ◽  
Ying-Hong Tang ◽  
Hua Li ◽  
Bei-Yang Chen ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Extracellular Matrix ◽  
Intimal Hyperplasia ◽  
Panax Notoginseng ◽  
Cell Cycle Protein

scholarly journals Fibroblast senescence in the pathology of idiopathic pulmonary fibrosis

2018 ◽  
Vol 315 (2) ◽  
pp. L162-L172 ◽  
Author(s):  
David W. Waters ◽  
Kaj E. C. Blokland ◽  
Prabuddha S. Pathinayake ◽  
Janette K. Burgess ◽  
Steven E. Mutsaers ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Wound Repair ◽  
Cell Cycle Progression ◽  
Early Stage ◽  
Critical Role ◽  
Cell Types ◽  
Normal Fibroblast ◽  
Cycle Arrest

Idiopathic pulmonary fibrosis (IPF) is a chronic fibrosing interstitial pneumonia of unknown cause with a median survival of only three years. Little is known about the mechanisms that precede the excessive collagen deposition seen in IPF, but cellular senescence has been strongly implicated in disease pathology. Senescence is a state of irreversible cell-cycle arrest accompanied by an abnormal secretory profile and is thought to play a critical role in both development and wound repair. Normally, once a senescent cell has contributed to wound repair, it is promptly removed from the environment via infiltrating immune cells. However, if immune clearance fails, the persistence of senescent cells is thought to drive disease pathology through their altered secretory profile. One of the major cell types involved in wound healing is fibroblasts, and senescent fibroblasts have been identified in the lungs of patients with IPF and in fibroblast cultures from IPF lungs. The question of what is driving abnormally high numbers of fibroblasts into senescence remains unanswered. The transcription factor signal transducer and activator of transcription 3 (STAT3) plays a role in a myriad of processes, including cell-cycle progression, gene transcription, as well as mitochondrial respiration, all of which are dysregulated during senescence. Activation of STAT3 has previously been shown to correlate with IPF progression and therefore is a potential molecular target to modify early-stage senescence and restore normal fibroblast function. This review summarizes what is presently known about fibroblast senescence in IPF and how STAT3 may contribute to this phenotype.

scholarly journals The DNA deaminase APOBEC3B interacts with the cell-cycle protein CDK4 and disrupts CDK4-mediated nuclear import of Cyclin D1

2019 ◽  
Vol 294 (32) ◽  
pp. 12099-12111 ◽  
Author(s):  
Jennifer L. McCann ◽  
Madeline M. Klein ◽  
Evelyn M. Leland ◽  
Emily K. Law ◽  
William L. Brown ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cyclin D1 ◽  
Nuclear Import ◽  
Cell Cycle Protein

scholarly journals IGF-I mediates regeneration of endocrine pancreas by increasing beta cell replication through cell cycle protein modulation in mice

Diabetologia ◽  
2008 ◽  
Vol 51 (10) ◽  
pp. 1862-1872 ◽  
Author(s):  
J. Agudo ◽  
E. Ayuso ◽  
V. Jimenez ◽  
A. Salavert ◽  
A. Casellas ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Beta Cell ◽  
Endocrine Pancreas ◽  
Cell Replication ◽  
Cell Cycle Protein ◽  
Beta Cell Replication ◽  
Igf I

Evaluation of proliferative index and cell cycle protein expression in choroid plexus tumors in children

2001 ◽  
Vol 103 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Carlos G. Carlotti ◽  
Bodour Salhia ◽  
Sheila Weitzman ◽  
Mark Greenberg ◽  
Peter B. Dirks ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Expression ◽  
Choroid Plexus ◽  
Proliferative Index ◽  
Cell Cycle Protein ◽  
Choroid Plexus Tumors
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