Polyelectrolyte Multi-Layered Griseofulvin Nanoparticles: Conventional versus Continuous In-Situ Layer-by-Layer Fabrication

2021 ◽  
Vol 21 (11) ◽  
pp. 5611-5621
Author(s):  
Sumayah Abdul-Jabbar ◽  
Gary P. Martin ◽  
Luigi G. Martini ◽  
Jayne Lawrence ◽  
Paul G. Royall
Keyword(s):  
Conventional Method ◽  
Electrostatic Interactions ◽  
De Novo ◽  
Particle Surface ◽  
Drug Carriers ◽  
Water Soluble ◽  
Layer By Layer ◽  
Polyelectrolyte Multilayer ◽  
Potential Applications

Polyelectrolyte multilayers are promising drug carriers with potential applications in the delivery of poorly soluble drugs. Furthermore, the polyelectrolyte multilayer contributes towards electrostatic interactions, which enhances the physical and chemical stability of colloids when compared to those prepared by other approaches. The aim of this work was to generate a polyelectrolyte multilayer on well characterised nanoparticles of the poorly water-soluble drug, griseofulvin. Griseofulvin (GF) nanoparticles (300 nm) were produced by wet bead milling, bearing a negative surface charge due to the use of poly(sodium 4-styrenesulfonate) (PSS) as a stabiliser. Six further layers of alternating chitosan and PSS polyelectrolyte multilayer were successfully generated at the particle surface either via use of: (1) the conventional method of adding excess coating polymer followed by centrifugation, or (2) the continuous in situ approach of adding sufficient amount of coating polymer. The continuous in situ method was designed de novo by the consecutive addition of polymers under high shear rate mixing. In comparison to the continuous in situ method, the conventional method yielded nanoparticles of smaller size (282 ±9 nm vs. 497 ±34 nm) and higher stability by maintaining its size for 6 months. In conclusion, the parent griseofulvin nanosuspension proved to be a suitable candidate for the polyelectrolyte multilayer fabrication providing an avenue for a bespoke formulation with versatile and potentially enhanced drug delivery properties.

scholarly journals Mucosal Applications of Poloxamer 407-Based Hydrogels: An Overview

Pharmaceutics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 159 ◽  
Author(s):  
Elena Giuliano ◽  
Donatella Paolino ◽  
Massimo Fresta ◽  
Donato Cosco
Keyword(s):  
Triblock Copolymer ◽  
Room Temperature ◽  
Thermal Characteristic ◽  
Drug Carriers ◽  
Water Soluble ◽  
Poloxamer 407 ◽  
Hydrophobic Residue ◽  
Pharmaceutical Drug ◽  
Mucosal Drug Delivery

Poloxamer 407, also known by the trademark Pluronic® F127, is a water-soluble, non-ionic triblock copolymer that is made up of a hydrophobic residue of polyoxypropylene (POP) between the two hydrophilic units of polyoxyethylene (POE). Poloxamer 407-based hydrogels exhibit an interesting reversible thermal characteristic. That is, they are liquid at room temperature, but they assume a gel form when administered at body temperature, which makes them attractive candidates as pharmaceutical drug carriers. These systems have been widely investigated in the development of mucoadhesive formulations because they do not irritate the mucosal membranes. Based on these mucoadhesive properties, a simple administration into a specific compartment should maintain the required drug concentration in situ for a prolonged period of time, decreasing the necessary dosages and side effects. Their main limitations are their modest mechanical strength and, notwithstanding their bioadhesive properties, their tendency to succumb to rapid elimination in physiological media. Various technological approaches have been investigated in the attempt to modulate these properties. This review focuses on the application of poloxamer 407-based hydrogels for mucosal drug delivery with particular attention being paid to the latest published works.

GOLD NANOPARTICLE-INCORPORATED POLYELECTROLYTE MULTILAYER FOR SENSITIVE ELECTROCHEMICAL IMMUNOSENSING

COSMOS ◽  
2010 ◽  
Vol 06 (02) ◽  
pp. 197-205
Author(s):  
WEIHUA HU ◽  
CHANG MING LI
Keyword(s):  
Storage Stability ◽  
Dynamic Range ◽  
Layer By Layer ◽  
Polyelectrolyte Multilayer ◽  
Redox Species ◽  
Protein Resistance ◽  
Amperometric Immunosensor ◽  
Capture Antibody ◽  
Electrochemical Immunosensing

Reported was a novel amperometric immunosensor based on layer-by-layer (LbL) assembled polyethylenimine/gold nanoparticles/poly (acrylic acid) (PEI/AuNPs/PAA) multilayer. The assembly process was in situ monitored by surface plasmon resonance (SPR) technique. Anti-goat IgG as a capture antibody was covalently immobilized on the outermost PAA layer of the multilayer to construct an immunosensor. A target protein, goat IgG was electrochemically detected with alkaline phosphatase-conjugated anti-goat IgG (ALP-anti-goat IgG) as a recognition antibody. Electrochemical investigations suggest that the incorporation of AuNPs facilitates the electron transfer between the underlying electrode and the redox species in solution, and thus enhances the electrochemical signals and in turn improves the immunosensing performance. A detection limit of 100 pg mL-1 with a dynamic range of five orders of magnitude was achieved. Due to the protein-friendly environment and the protein resistance of the polyelectrolyte multilayer, the resulting immunosensor demonstrates excellent storage stability, satisfying assay specificity.

scholarly journals Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1230
Author(s):  
Ekaterina Naumenko ◽  
Farida Akhatova ◽  
Elvira Rozhina ◽  
Rawil Fakhrullin
Keyword(s):  
Gene Delivery ◽  
Electrostatic Interactions ◽  
Surface Engineering ◽  
Delivery Systems ◽  
In Vitro Cytotoxicity ◽  
Water Soluble ◽  
Layer By Layer ◽  
Dependent Manner ◽  
Cationic Polyelectrolytes

Polycations are an essential part of layer-by-layer (LbL)-assembled drug delivery systems, especially for gene delivery. In addition, they are used for other related applications, such as cell surface engineering. As a result, an assessment of the cytotoxicity of polycations and elucidation of the mechanisms of polycation toxicity is of paramount importance. In this study, we examined in detail the effects of a variety of water-soluble, positively charged synthetic polyelectrolytes on in vitro cytotoxicity, cell and nucleus morphology, and monolayer expansion changes. We have ranked the most popular cationic polyelectrolytes from the safest to the most toxic in relation to cell cultures. 3D cellular cluster formation was disturbed by addition of polyelectrolytes in most cases in a dose-dependent manner. Atomic force microscopy allowed us to visualize in detail the structures of the polyelectrolyte–DNA complexes formed due to electrostatic interactions. Our results indicate a relationship between the structure of the polyelectrolytes and their toxicity, which is necessary for optimization of drug and gene delivery systems.

Recent Advances of Water-Soluble Fullerene Derivatives in Biomedical Applications

2018 ◽  
Vol 16 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Xiaoyan Zhang ◽  
Hailin Cong ◽  
Bing Yu ◽  
Qun Chen
Keyword(s):  
Biomedical Applications ◽  
Antiviral Agents ◽  
Drug Carriers ◽  
Water Soluble ◽  
Covalent Bonds ◽  
Fullerene Derivatives ◽  
Dynamic Therapy ◽  
Potential Applications ◽  
Biomedical Field ◽  
Photo Dynamic Therapy

Fullerenes, especially C60, exhibit great potential applications in biology field, due to their excellent antibacterial, antiviral, antitumor and other properties. Many water-soluble fullerene derivatives have been designed, synthesized and used as photo-dynamic therapy agents, antiviral agents, bioimaging agents, drug carriers and so on. This review focuses on the usage of water-soluble fullerene derivatives in biomedical field in recent years. The first half gives the preparation of water-soluble fullerene derivatives themselves with covalent bonds and non-covalent bonds. In the second half, myriad applications of water-soluble fullerene derivatives in biomedical field are introduced.

scholarly journals A Fluorescence Sensing Determination of 2, 4, 6-Trinitrophenol Based on Cationic Water-Soluble Pillar[6]arene Graphene Nanocomposite

Sensors ◽  
2018 ◽  
Vol 19 (1) ◽  
pp. 91 ◽  
Author(s):  
Xiaoping Tan ◽  
Tingying Zhang ◽  
Wenjie Zeng ◽  
Shuhua He ◽  
Xi Liu ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Fluorescence Probe ◽  
Water Soluble ◽  
Fluorescence Sensing ◽  
Sensing Platform ◽  
Reduced Graphene ◽  
Potential Applications ◽  
Water And Soil Samples ◽  
Graphene Nanocomposite

We describe a selective and sensitive fluorescence platform for the detection of trinitrophenol (TNP) based on competitive host–guest recognition between pyridine-functionalized pillar[6]arene (PCP6) and a probe (acridine orange, AO) that used PCP6-functionalized reduced graphene (PCP6-rGO) as the receptor. TNP is an electron-deficient and negative molecule, which is captured by PCP6 via electrostatic interactions and π–π interactions. Therefore, a selective and sensitive fluorescence probe for TNP detection is developed. It has a low detection limit of 0.0035 μM (S/N = 3) and a wider linear response of 0.01–5.0 and 5.0–125.0 for TNP. The sensing platform is also used to test TNP in two water and soil samples with satisfying results. This suggests that this approach has potential applications for the determination of TNP.

scholarly journals Layer-by-Layer Assembly of Polyelectrolyte Multilayer onto PET Fabric for Highly Tunable Dyeing with Water Soluble Dyestuffs

Polymers ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 735 ◽  
Author(s):  
Shili Xiao ◽  
Pengjun Xu ◽  
Qingyan Peng ◽  
Jiali Chen ◽  
Jiankang Huang ◽  
...  
Keyword(s):  
Water Soluble ◽  
Layer By Layer ◽  
Polyelectrolyte Multilayer ◽  
Pet Fabric ◽  
Layer By Layer Assembly ◽  
Layer Assembly

Molecular Layer-by-Layer Self-Assembly of Water-Soluble Perylene Diimides through π−π and Electrostatic Interactions

Langmuir ◽  
2006 ◽  
Vol 22 (1) ◽  
pp. 26-28 ◽  
Author(s):  
Tingji Tang ◽  
Jiangqiang Qu ◽  
Klaus Müllen ◽  
Stephen E. Webber
Keyword(s):  
Self Assembly ◽  
Electrostatic Interactions ◽  
Molecular Layer ◽  
Water Soluble ◽  
Layer By Layer ◽  
Perylene Diimides

scholarly journals A Facile and High Selectivity Novel Fluorescence Sensing Determination of 2, 4, 6-Trinitrophenol Based on Cationic Water-Soluble Pillar[6]arene Graphene Nanocomposite

2018 ◽  
Author(s):  
Xiaoping Tan ◽  
Tingying Zhang ◽  
Wenjie Zeng ◽  
Shuhua He ◽  
Xi Liu ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Water Soluble ◽  
Fluorescence Sensing ◽  
Sensing Platform ◽  
Reduced Graphene ◽  
Potential Applications ◽  
Water And Soil Samples ◽  
Graphene Nanocomposite ◽  
Pi Interactions

We describe a selective and sensitive fluorescence platform for the detection of trinitrophenol (TNP) based on competitive host–guest recognition between pyridine-functionalized pillar[6]arene (PCP6) and probe (acridine orange, AO) that used PCP6-functionalized reduced graphene (PCP6-rGO) as the receptor. TNP is an electron-deficient and negative molecule which is captured by PCP6 via electrostatic interactions and π-π interactions. Therefore, a selective and sensitive fluorescence sensor for TNP detection is developed. It has a low detection limit of 0.0035 μM (S/N=3) and a wider linear response of 0.01−5.0 and 5.0−125.0 for TNP. The sensing platform is also used to test TNP in two water and soil samples with satisfying results. This suggests that this approach has potential applications for the determination of TNP.

An in situ and ex situ TEM study of the formation of thin cobalt silicide films by molecular beam epitaxy on the silicon (100) surface

1988 ◽  
Vol 46 ◽  
pp. 884-885
Author(s):  
D. Loretto ◽  
J. M. Gibson ◽  
S. M. Yalisove ◽  
R. T. Tung
Keyword(s):  
Molecular Beam Epitaxy ◽  
Molecular Beam ◽  
Defect Density ◽  
High Vacuum ◽  
Ultra High Vacuum ◽  
Ex Situ ◽  
Metal Base ◽  
In Situ Experiments ◽  
Potential Applications

The cobalt disilicide/silicon system has potential applications as a metal-base and as a permeable-base transistor. Although thin, low defect density, films of CoSi2 on Si(111) have been successfully grown, there are reasons to believe that Si(100)/CoSi2 may be better suited to the transmission of electrons at the silicon/silicide interface than Si(111)/CoSi2. A TEM study of the formation of CoSi2 on Si(100) is therefore being conducted. We have previously reported TEM observations on Si(111)/CoSi2 grown both in situ, in an ultra high vacuum (UHV) TEM and ex situ, in a conventional Molecular Beam Epitaxy system.The procedures used for the MBE growth have been described elsewhere. In situ experiments were performed in a JEOL 200CX electron microscope, extensively modified to give a vacuum of better than 10-9 T in the specimen region and the capacity to do in situ sample heating and deposition. Cobalt was deposited onto clean Si(100) samples by thermal evaporation from cobalt-coated Ta filaments.

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