Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy

2016 ◽  
Vol 70 (10) ◽  
pp. 1639-1648 ◽  
Author(s):  
Jinghua Liu ◽  
Qing Huang
Keyword(s):  
Fourier Transform ◽  
High Throughput Screening ◽  
Haematococcus Pluvialis ◽  
Fourier Transform Infrared ◽  
Raman Microspectroscopy ◽  
Screening Methods ◽  
Ir Absorption ◽  
New Approach ◽  
Ft Ir ◽  
Ir And Raman

Haematococcus pluvialis has promising applications owing to its ability to accumulate astaxanthin under stress conditions. In order to acquire higher astaxanthin productivity from H. pluvialis, it is critical not only to develop efficient mutagenesis techniques, but also to establish rapid and effective screening methods which are highly demanded in current research and application practice. In this work, we therefore attempted to develop a new approach to screening the astaxanthin-hyperproducing strains based on spectroscopic tools. Using Fourier transform infrared (FT-IR) and Raman microspectroscopy, we have achieved rapid and quantitative analysis of the algal cells in terms of astaxanthin, β-carotene, proteins, lipids, and carbohydrates. In particular, we have found that the ratio of the IR absorption band at 1740 cm−1 to the band at 1156 cm−1 can be utilized for identifying astaxanthin-hyperproducing strains. This work may therefore open a new avenue for developing high-throughput screening methods necessary for the microbial mutant breeding industry.

Revealing Covariance Structures in Fourier Transform Infrared and Raman Microspectroscopy Spectra: A Study on Pork Muscle Fiber Tissue Subjected to Different Processing Parameters

2007 ◽  
Vol 61 (10) ◽  
pp. 1032-1039 ◽  
Author(s):  
Ulrike Böcker ◽  
Ragni Ofstad ◽  
Zhiyun Wu ◽  
Hanne Christine Bertram ◽  
Ganesh D. Sockalingum ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Fourier Transform ◽  
Raman Spectra ◽  
Muscle Tissue ◽  
Salt Content ◽  
Fourier Transform Infrared ◽  
Spectroscopic Techniques ◽  
Ft Ir ◽  
Ir And Raman ◽  
Ir Bands

The aim of this study was to investigate the correlation patterns between Fourier transform infrared (FT-IR) and Raman microspectroscopic data obtained from pork muscle tissue, which helped to improve the interpretation and band assignment of the observed spectral features. The pork muscle tissue was subjected to different processing factors, including aging, salting, and heat treatment, in order to induce the necessary degree of variation of the spectra. For comparing the information gained from the two spectroscopic techniques with respect to the experimental design, multiblock principal component analysis (MPCA) was utilized for data analysis. The results showed that both FT-IR and Raman spectra were mostly affected by heat treatment, followed by the variation in salt content. Furthermore, it could be observed that IR amide I, II, and III band components appear to be effected to a different degree by brine-salting and heating. FT-IR bands assigned to specific protein secondary structures could be related to different Raman C–C stretching bands. The Raman C–C skeletal stretching bands at 1031, 1061, and 1081 cm−1 are related to the IR bands indicative of aggregated β-structures, while the Raman bands at 901 cm−1 and 934 cm−1 showed a strong correlation with IR bands assigned to α-helical structures. At the same time, the IR band at 1610 cm−1, which formerly was assigned to tyrosine in spectra originating from pork muscle, did not show a correlation to the strong tyrosine doublet at 827 and 852 cm−1 found in Raman spectra, leading to the conclusion that the IR band at 1610 cm−1 found in pork muscle tissue is not originating from tyrosine.

Forensic Analysis of Architectural Finishes Using Fourier Transform Infrared and Raman Spectroscopy, Part II: White Paint

2005 ◽  
Vol 59 (11) ◽  
pp. 1340-1346 ◽  
Author(s):  
Steven E. J. Bell ◽  
Louise A. Fido ◽  
S. James Speers ◽  
W. James Armstrong ◽  
Sharon Spratt
Keyword(s):  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Forensic Analysis ◽  
The Other ◽  
Large Group ◽  
Ir Microscopy ◽  
Infrared And Raman Spectroscopy ◽  
Ft Ir ◽  
Ir And Raman

White household paints are commonly encountered as evidence in the forensic laboratory but they often cannot be readily distinguished by color alone so Fourier transform infrared (FT-IR) microscopy is used since it can sometimes discriminate between paints prepared with different organic resins. Here we report the first comparative study of FT-IR and Raman spectroscopy for forensic analysis of white paint. Both techniques allowed the 51 white paint samples in the study to be classified by inspection as either belonging to distinct groups or as unique samples. FT-IR gave five groups and four unique samples; Raman gave seven groups and six unique samples. The basis for this discrimination was the type of resin and/or inorganic pigments/extenders present. Although this allowed approximately half of the white paints to be distinguished by inspection, the other half were all based on a similar resin and did not contain the distinctive modifiers/pigments and extenders that allowed the other samples to be identified. The experimental uncertainty in the relative band intensities measured using FT-IR was similar to the variation within this large group, so no further discrimination was possible. However, the variation in the Raman spectra was larger than the uncertainty, which allowed the large group to be divided into three subgroups and four distinct spectra, based on relative band intensities. The combination of increased discrimination and higher sample throughput means that the Raman method is superior to FT-IR for samples of this type.

High-Throughput Analyses of Microplastic Samples Using Fourier Transform Infrared and Raman Spectrometry

2020 ◽  
Vol 74 (9) ◽  
pp. 1185-1197 ◽  
Author(s):  
Josef Brandt ◽  
Lars Bittrich ◽  
Franziska Fischer ◽  
Elisavet Kanaki ◽  
Alexander Tagg ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Open Source Software ◽  
Fourier Transform Infrared ◽  
Particle Analysis ◽  
Particle Detection ◽  
Raman Spectrometry ◽  
Open Source Software Package ◽  
Ft Ir ◽  
Ir Microspectroscopy ◽  
Ir And Raman

Determining microplastics in environmental samples quickly and reliably is a challenging task. With a largely automated combination of optical particle analysis, Fourier transform infrared (FT-IR), and Raman microscopy along with spectral database search, particle sizes, particle size distributions, and the type of polymer including particle color can be determined. We present a self-developed, open-source software package for realizing a particle analysis approach with both Raman and FT-IR microspectroscopy. Our software GEPARD (Gepard Enabled PARticle Detection) allows for acquiring an optical image, then detects particles and uses this information to steer the spectroscopic measurement. This ultimately results in a multitude of possibilities for efficiently reviewing, correcting, and reporting all obtained results.

scholarly journals Plasma Protein Contents Determined by Fourier-Transform Infrared Spectrometry

2001 ◽  
Vol 47 (4) ◽  
pp. 730-738 ◽  
Author(s):  
Cyril Petibois ◽  
Georges Cazorla ◽  
André Cassaigne ◽  
Gérard Déléris
Keyword(s):  
Fourier Transform ◽  
Ir Spectra ◽  
Spectral Range ◽  
Plasma Proteins ◽  
Fourier Transform Infrared ◽  
Infrared Spectrometry ◽  
Ir Absorption ◽  
Apo B ◽  
Ir Spectrometry ◽  
Ft Ir

Abstract Background: Fourier-transform infrared (FT-IR) spectrometry has been used to measure small molecules in plasma. We wished to extend this use to measurement of plasma proteins. Methods: We analyzed plasma proteins, glucose, lactate, and urea in 49 blood samples from 35 healthy subjects and 14 patients. For determining the concentration of each biomolecule, the method used the following steps: (a) The biomolecule was sought for which the correlation between spectral range areas of plasma FT-IR spectra and concentrations determined by comparison method was greatest. (b) The IR absorption of the biomolecule at the most characteristic spectral range was calculated by analyzing pure samples of known concentrations. (c) The plasma concentration of the biomolecule was determined using the FT-IR absorption of the pure compound and the integration value obtained for the plasma FT-IR spectra. (d) The spectral contribution of the biomolecule was subtracted from the plasma FT-IR spectra, and the resulting spectra were saved for further analyses. (e) The same method was then applied to determining the concentrations of other biomolecules by sequentially comparing the resulting FT-IR spectra. Results: Results agreed with those obtained by clinical methods for the following biomolecules when analyzed in the following order: albumin, glucose, fibrinogen, IgG2, lactate, IgG1, α1-antitrypsin, α2-macroglobulin, transferrin, apolipoprotein (Apo)-A1, urea, Apo-B, IgM, Apo-C3, IgA, IgG4, IgG3, IgD, haptoglobin, and α1-acid glycoprotein. Conclusion: FT-IR spectrometry is a useful tool for determining concentrations of several plasma biomolecules.

FR-IR Molecular Microanalysis System

1990 ◽  
Vol 48 (2) ◽  
pp. 270-271
Author(s):  
John A. Reffner ◽  
William T. Wihlborg
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Integrated System ◽  
Morphological Examination ◽  
Fully Integrated ◽  
Ir Microscopy ◽  
Normal Functions ◽  
Infrared Microspectroscopy ◽  
Infrared Spectral ◽  
Ft Ir

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

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