Zinc Concentrations in Pure Populations of Peripheral Blood Neutrophils, Lymphocytes and Monocytes

1989 ◽  
Vol 26 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Helen F Goode ◽  
J Kelleher ◽  
B E Walker
Keyword(s):  
Peripheral Blood ◽  
Zinc Supplementation ◽  
Serum Zinc ◽  
Normal Subjects ◽  
Cell Populations ◽  
Zinc Status ◽  
Blood Neutrophils ◽  
Zinc Concentrations ◽  
Cellular Zinc

It is doubtful if the measurement of plasma or serum zinc is of value in assessing zinc status. Leucocyte zinc has been suggested as an alternative since it may be representative of tissue zinc stores; but in many studies poorly defined cell populations make interpretation difficult. This paper describes detailed techniques for the isolation and analysis of pure populations of neutrophils, lymphocytes and monocytes. Zinc concentrations (± 1SD) in normal subjects were 1·;26 ± 0·;27 nmol/mg protein, 1·;85 ± 0·;32 nmol/mg protein and 2·;58 ±0·;65 nmol/mg protein in neutrophils, lymphocytes and monocytes respectively. Fasting caused a significant decrease in neutrophil and lymphocyte zinc, and an increase in monocyte zinc. Supplementation of zinc-replete subjects with 135 mg zinc/day for 3 weeks had no significant effect on cellular zinc concentrations.

scholarly journals Effect of Zinc Supplementation on Serum Zinc Level and Micronucleus Frequency in Bangladeshi Adult Females With Poor Socioeconomic Status

1970 ◽  
Vol 1 (1) ◽  
pp. 4-8
Author(s):  
Tarek Golam Mustafa ◽  
Md Monirujjaman ◽  
Shahana Zabeen ◽  
Bakhtiar Hossain
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Zinc Supplementation ◽  
Serum Zinc ◽  
Zinc Status ◽  
Peripheral Blood Mononuclear ◽  
Micronucleus Frequency ◽  
Blood Mononuclear Cells ◽  
Zinc Levels

Zinc deficiency may result in increased DNA oxidation resulting in DNA breaks which leads to micronuclei formation. Therefore, micronuclei frequency in peripheral blood mononuclear cells has the potential to be an indicator for zinc status. The study was designed to explore the possibility of using micronuclei frequency (MNF) in peripheral blood mononuclear cells (PBMCs), as a biomarker for zinc status. The study was double blind and placebo controlled. Fourteen females with moderately low dietary zinc intake were randomly assigned to receive either zinc (20 mg of zinc as zinc sulfate /day) or placebo for twenty-one days. MNF of peripheral blood lymphocytes were determined by using cytokinesis-block micronucleus (CBMN) assay. Plasma zinc levels were measured using atomic absorption spectrophotometry. Zinc supplementation increased serum zinc levels (P = 0.008) and decreased cells with micronucleus (P =0.054) and micronuleus frequency (P = 0.016) in PBMCs. Individuals with higher zinc status, as achieved with zinc supplementation, have low micronucleus frequency. Keywords: MNF (Micronucleus Frequency); CBMN (Cytokinesis Block Micronucleus) Assay; PBMC (Peripheral Blood Mononuclear Cells) DOI: 10.3329/akmmcj.v1i1.7452 Anwer Khan Modern Medical College Journal 2010; 1(1): 04-08

Use of Serum Zinc Concentration as an Indicator of Population Zinc Status

2007 ◽  
Vol 28 (3_suppl3) ◽  
pp. S403-S429 ◽  
Author(s):  
Sonja Y. Hess ◽  
Janet M. Peerson ◽  
Janet C. King ◽  
Kenneth H. Brown
Keyword(s):  
Zinc Deficiency ◽  
Zinc Concentration ◽  
Zinc Supplementation ◽  
Serum Zinc ◽  
Interindividual Variation ◽  
Zinc Status ◽  
Dietary Zinc ◽  
Serum Zinc Concentration ◽  
Zinc Concentrations ◽  
Intervention Trials

Assessing the prevalence and severity of zinc deficiency in populations is critical to determine the need for and appropriate targeting of zinc intervention programs and to assess their effectiveness for improving the health and well-being of high-risk populations. However, there is very little information on the zinc status of populations worldwide due to the lack of consensus on appropriate biochemical indicators of zinc status. The objective of this review was to evaluate the use of serum zinc concentration as an indicator of population zinc status. We have reviewed the response of serum zinc concentration to dietary zinc restriction and zinc supplementation. In addition, we completed pooled analyses of nine zinc intervention trials in young children to assess the relations between serum zinc concentration of individuals before treatment and their responses to zinc supplementation. Also, in updated combined analyses of previously published data, we investigated the relation between the mean initial serum zinc concentration of a study population and their mean growth responses to zinc supplementation in randomized intervention trials among children. The results from depletion/repletion studies indicate that serum zinc concentrations respond appreciably to severe dietary zinc restriction, although there is considerable interindividual variation in these responses. There is also clear evidence that both individual and population mean serum zinc concentrations increase consistently during zinc supplementation, regardless of the initial level of serum zinc concentration. By contrast, an individual's serum zinc concentration does not reliably predict that person's response to zinc supplementation. Serum zinc concentration can be considered a useful biomarker of a population's risk of zinc deficiency and response to zinc interventions, although it may not be a reliable indicator of individual zinc status.

scholarly journals Indicators of zinc status at the population level: a review of the evidence

2008 ◽  
Vol 99 (S3) ◽  
pp. S14-S23 ◽  
Author(s):  
Rosalind S. Gibson ◽  
Sonja Y. Hess ◽  
Christine Hotz ◽  
Kenneth H. Brown
Keyword(s):  
Serum Zinc ◽  
Population Level ◽  
Molecular Techniques ◽  
Collaborative Group ◽  
Zinc Status ◽  
Turnover Rates ◽  
Functional Responses ◽  
Zinc Metalloenzymes ◽  
Zinc Concentrations ◽  
New Biomarkers

The role of zinc deficiency as an important cause of morbidity and impaired linear growth has prompted the need to identify indicators of population zinc status. Three indicators have been recommended – prevalence of zinc intakes below the estimated average requirement (EAR), percentage with low serum zinc concentrations, and percentage of children aged < 5 years who are stunted. This review outlines steps to estimate the prevalence of inadequate intakes, and confirm their validity based on the EARs set by International Zinc Nutrition Collaborative Group. Next, the appropriateness of serum zinc as a biochemical marker for population zinc status is confirmed by a summary of: (a) the response of serum zinc concentrations to zinc intakes; (b) usefulness of serum zinc concentrations to predict functional responses to zinc interventions; (c) relationship between initial serum zinc and change in serum zinc in response to interventions. Height- or length-for-age was chosen as the best functional outcome after considering the responses of growth, infectious diseases (diarrhoea, pneumonia), and developmental outcomes in zinc supplementation trials and correlation studies. The potential of other zinc biomarkers such as zinc concentrations in hair, cells, zinc-metalloenzymes, and zinc-binding proteins, such as metallothionein, is also discussed. Molecular techniques employing reverse transcriptase (RT)-polymerase chain reaction to measure mRNA in metallothionein and ZIP1 transporter hold promise, as do kinetic markers such as exchangeable zinc pools (EZP) and plasma zinc turnover rates. More research is needed to establish the validity, specificity, sensitivity, and feasibility of these new biomarkers, especially in community-settings.

scholarly journals Concurrent Training Decreases Cortisol but Not Zinc Concentrations: Effects of Distinct Exercise Protocols

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Guilherme Rosa ◽  
Marcos de Sá Rego Fortes ◽  
Danielli B. de Mello
Keyword(s):  
Strength Training ◽  
Serum Zinc ◽  
Serum Cortisol ◽  
Concurrent Training ◽  
Zinc Status ◽  
Study Session ◽  
Zinc Concentrations ◽  
Cortisol Levels ◽  
Male Subjects ◽  
Blood Variables

Objectives. To investigate the effects of distinct concurrent training (CT) protocols on zinc and cortisol concentrations and test the correlation between these blood variables.Methods. Samples of serum zinc and cortisol were assessed from 10 male subjects (27.1±4.8years old; BMI25.38±0.09) before and immediately after each study session: control (CS = no exercises), concurrent training 1 (CT1 = indoor cycling + strength training), and concurrent training 2 (CT2 = strength training + indoor cycle) with five days of interval between each.Results. There were no significant changes in zinc concentrations after the CS (Δ% = 8.45;p=0.07), CT1 (Δ% = 4.77;p=0.49), and CT2 (Δ% = −2.90;p=0.12) sessions. Cortisol levels showed significant decrease after CS (Δ% = −6.02;p=0.00), CT1 (Δ% = −26.32;p=0.02), and CT2 (Δ% = −33.57;p=0.05) sessions. There was a significant correlation between the variables only at CS (zinc post versus cortisol pre:r=0.82and cortisol post:r=0.82).Conclusions. CT decreases cortisol concentrations regardless of the sequence performed. No changes were found in zinc concentrations after the study sessions. The reduction in serum cortisol concentrations appear to occur by a mechanism independent of the zinc status.

Serum zinc status in thalassemic adolescents attending Yangon Children Hospital, Myanmar

2020 ◽  
Author(s):  
Win-Yu Aung ◽  
Thae-Nu Htwe ◽  
Myat Thandar ◽  
Ohn Mar
Keyword(s):  
Zinc Deficiency ◽  
Day Care ◽  
Zinc Concentration ◽  
Zinc Supplementation ◽  
Care Center ◽  
Serum Zinc ◽  
Delayed Puberty ◽  
Zinc Status ◽  
Day Care Center ◽  
Serum Zinc Concentration

Background: Thalassemia constitutes a major public health problem causing a significant burden on children and their families. Zinc deficiency plays an important role in many thalassemia-related complications like growth retardation, hypogonadism and delayed puberty which are frequently noted in adolescent age. Although zinc is supplemented to thalassemic patients visiting Day Care Center, Yangon Children Hospital (YCH), Myanmar, a report concerning serum zinc level of these patients is still lacking. This study, therefore, aimed to assess serum zinc status in thalassemic adolescents attending Day Care Center, YCH. Materials and Methods: This hospital-based cross-sectional study was conducted on 99 thalassemic adolescents. Mean age of diagnosis was 5.1±2.1 years. Non-fasting serum zinc concentration was determined by atomic absorption spectrophotometry. According to National Health and Nutrition Examination Survey data, zinc deficiency was defined as serum zinc concentration < 66 μg/dL (female) and < 70 μg/dL (male). Results: Serum zinc concentration (μg/dL) was 57.35 (47.30-80.14) (median, interquartile range) with maximum, 195.05 and minimum, 28.83. Zinc deficiency was observed in 69.7% (69 out of 99; 35 males and 34 females) of the patients. The associations of zinc deficiency with gender, phenotype and the use of chelator were non-significant (P>0.05). Conclusion: In spite of zinc supplementation, nearly 70% of the thalassemic adolescents showed zinc deficiency. Zinc deficiency in these adolescents might not be related to gender, phenotypes or the use of chelator. Poor compliance to take zinc supplementation and/or irregular blood transfusion could partly be attributable to zinc deficiency in these adolescents. Providing health education on the importance of regular intake of adequate zinc is advisable and periodic evaluation of zinc levels is recommended for thalassemic adolescents.

Tissue zinc status and drug elimination in patients with chronic liver disease

1990 ◽  
Vol 78 (6) ◽  
pp. 547-549 ◽  
Author(s):  
Michael Barry ◽  
P. W. N. Keeling ◽  
John Feely
Keyword(s):  
Hepatic Cirrhosis ◽  
Serum Zinc ◽  
Zinc Content ◽  
Control Group ◽  
Zinc Status ◽  
Antipyrine Clearance ◽  
Zinc Depletion ◽  
Antipyrine Kinetics ◽  
Healthy Control ◽  
Zinc Concentrations

1. The zinc status and drug-metabolizing ability of 15 patients with histologically diagnosed hepatic cirrhosis were studied. Zinc status was assessed using both serum and leucocyte zinc concentrations, and drug-metabolizing ability was assessed by antipyrine kinetics. 2. Patients with cirrhosis were found to have lower serum and leucocyte zinc concentrations when compared with a healthy control group. 3. Leucocyte zinc content and antipyrine clearance were correlated. Those patients with the lowest leucocyte zinc content had the greatest impairment of drug metabolism. Antipyrine elimination and serum zinc concentrations were not correlated. 4. Leucocyte zinc concentrations and antipyrine clearance were not influenced by the severity of liver dysfunction, as assessed by using the Child Turcotte classification. 5. These results suggest that tissue zinc depletion in some patients with hepatic cirrhosis may explain in part the impaired capacity to metabolize drugs.

scholarly journals Effects of organic zinc supplementation in weaned calves

2016 ◽  
Vol 85 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Sylva Dresler ◽  
Josef Illek ◽  
Ladislav Zeman
Keyword(s):  
Metabolic Profile ◽  
Zinc Supplementation ◽  
Serum Zinc ◽  
Treated Group ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Sod Activity ◽  
Organic Zinc ◽  
Zinc Concentrations ◽  
Organic Zn

The aim of the study was to investigate the effect of organic zinc supplementation in calves on serum zinc (Zn) concentrations, selected metabolic profile indicators and serum immunoglobulin (Ig) concentrations. The trial included 2 groups (n = 10) of weaned female calves. The Zn-Methionin calves (group Zn-Met) were supplemented with 30 mg Zn-Met/kg dry matter (DM)/day (BIOPLEX® Zn, Alltech, USA) for 90 days; the control calves (group C) received the same diet without organic zinc supplementation. Compared to the control group, organic Zn treatment significantly increased serum Zn concentration (P < 0.05), superoxide dismutase (SOD) activity (P < 0.01) and total Ig (P < 0.01) in the group Zn-Met at the beginning (7 days from the start of Zn-Met supplementation) of the trial. At the end of the trial (day 90) serum total protein (TP) (P < 0.05), albumin (P < 0.01), urea (P < 0.01), SOD (P < 0.01), copper (Cu) (P < 0.05), Zn (P < 0.01) and Ig (P < 0.05) concentrations were significantly higher in the Zn-Met calves. In the control group alkaline phosphatase (ALP) activity was significantly (P < 0.01) higher on day 90. A positive correlation between zinc concentrations, ALP, and SOD activities in serum, and a negative correlation between zinc and copper concentrations were demonstrated. Dietary Zn-Met supplementation in calves markedly influenced the metabolic profile and serum immunoglobulin concentrations. Compared to the control group, the Zn supplemented group showed a significantly (P < 0.05) lower ALP and significantly greater SOD serum activity (P < 0.01) at the end of the trial. Total Ig concentrations were significantly higher in the Zn treated group (day 7: P < 0.01 vs. day 90: P < 0.05).

scholarly journals Zinc Inhibits Phosphate-Induced Vascular Calcification through TNFAIP3-Mediated Suppression of NF-κB

2018 ◽  
Vol 29 (6) ◽  
pp. 1636-1648 ◽  
Author(s):  
Jakob Voelkl ◽  
Rashad Tuffaha ◽  
Trang T.D. Luong ◽  
Daniel Zickler ◽  
Jaber Masyout ◽  
...  
Keyword(s):  
Vascular Calcification ◽  
Zinc Finger Protein ◽  
Zinc Supplementation ◽  
Serum Zinc ◽  
Serum Samples ◽  
Cardiovascular Morbidity And Mortality ◽  
Zinc Concentrations ◽  
Zinc Sensing ◽  
Functional Relevance ◽  
Zinc Levels

Background The high cardiovascular morbidity and mortality of patients with CKD may result in large part from medial vascular calcification, a process promoted by hyperphosphatemia and involving osteo-/chondrogenic transdifferentiation of vascular smooth muscle cells (VSMCs). Reduced serum zinc levels have frequently been observed in patients with CKD, but the functional relevance of this remains unclear.Methods We performed experiments in primary human aortic VSMCs; klotho-hypomorphic (kl/kl), subtotal nephrectomy, and cholecalciferol-overload mouse calcification models; and serum samples from patients with CKD.Results In cultured VSMCs, treatment with zinc sulfate (ZnSO4) blunted phosphate-induced calcification, osteo-/chondrogenic signaling, and NF-κB activation. ZnSO4 increased the abundance of zinc-finger protein TNF-α–induced protein 3 (TNFAIP3, also known as A20), a suppressor of the NF-κB pathway, by zinc-sensing receptor ZnR/GPR39-dependent upregulation of TNFAIP3 gene expression. Silencing of TNFAIP3 in VSMCs blunted the anticalcific effects of ZnSO4 under high phosphate conditions. kl/kl mice showed reduced plasma zinc levels, and ZnSO4 supplementation strongly blunted vascular calcification and aortic osteoinduction and upregulated aortic Tnfaip3 expression. ZnSO4 ameliorated vascular calcification in mice with chronic renal failure and mice with cholecalciferol overload. In patients with CKD, serum zinc concentrations inversely correlated with serum calcification propensity. Finally, ZnSO4 ameliorated the osteoinductive effects of uremic serum in VSMCs.Conclusions Zinc supplementation ameliorates phosphate-induced osteo-/chondrogenic transdifferentiation of VSMCs and vascular calcification through an active cellular mechanism resulting from GPR39-dependent induction of TNFAIP3 and subsequent suppression of the NF-κB pathway. Zinc supplementation may be a simple treatment to reduce the burden of vascular calcification in CKD.

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