scholarly journals Angioarchitecture of Primary Oral Malignant Melanomas

2002 ◽  
Vol 50 (11) ◽  
pp. 1555-1562 ◽  
Author(s):  
You-Jin Lee ◽  
Noriyuki Nagai ◽  
Chong-Huat Siar ◽  
Keisuke Nakano ◽  
Hitoshi Nagatsuka ◽  
...  
Keyword(s):  
Malignant Tumors ◽  
Periodic Acid ◽  
Vasculogenic Mimicry ◽  
Tumor Vessel ◽  
Periodic Acid Schiff ◽  
Endothelial Markers ◽  
Tumor Microvasculature ◽  
Transmission Electron ◽  
Oral Malignant Melanoma ◽  
Vascular Channels

Angiogenesis is an essential process in the progression of malignant tumors. However, little is known of the angioarchitecture in primary oral malignant melanoma. We sought to determine this by the use of periodic acid-Schiff (PAS) stain, endothelial markers (CD34, CD105) and laminin, and by transmission electron microscopy in two cases. The results demonstrated that endothelium-lined vessels dominated the tumor microvasculature and these stained positively for PAS, laminin, and endothelial markers. Mosaic and tumorlined vessels were infrequently encountered. Most PAS-positive patterned networks and loops ultrastructurally represented intratumor microhemorrhages that probably arose secondary to tumor vessel leakiness. Vascular channels of the vasculogenic mimicry type were rare. They stained for laminin but not for endothelial markers.

scholarly journals Vasculogenic Mimicry Correlates to Presenting Symptoms and Mortality in Uveal Melanoma

2021 ◽  
Author(s):  
Shiva Sabazade ◽  
Viktor Torgny Gill ◽  
Christina Herrspiegel ◽  
Gustav Stålhammar
Keyword(s):  
Visual Field ◽  
Uveal Melanoma ◽  
Poor Prognosis ◽  
Periodic Acid ◽  
Vasculogenic Mimicry ◽  
Clinicopathological Parameters ◽  
Tumor Diameter ◽  
Blurred Vision ◽  
Periodic Acid Schiff ◽  
Presenting Symptoms

Abstract PurposeFluid-conducting extracellular matrix patterns known as vasculogenic mimicry (VM) have been associated with poor prognosis in uveal melanoma and other cancers. We investigate the correlations between VM, presenting symptoms, mortality and the area density of periodic acid-Schiff positive histological patterns (PAS density).MethodsSixty-nine patients that underwent enucleation for uveal melanoma between 2000 and 2007 were included. Clinicopathological parameters, presenting symptoms and outcomes were collected. Histological tumor sections were evaluated for VM and PAS density was quantified with digital image analysis.ResultsThirty-four patients (49 %) presented with blurred vision. 18 (26 %) with a shadow in the visual field, 7 (10 %) with photopsia and/or floaters and 2 (3 %) with metamorphopsia. Nine patients (13 %) had no symptoms at all. Median follow-up was 16.7 years (SD 2.6). A shadow in the visual field, but no other symptom, was positively correlated with the presence of VM (φ 0.70, p<0.001) and greater PAS density (p<0.001). In multivariate regression, retinal detachment (RD), presence of VM and PAS density ≥ median were independent predictors of a shadow, but not tumor distance to the macula, tumor apical thickness, tumor diameter or ciliary body engagement. Presence of VM was associated with significantly shorter cumulative disease-specific survival (Wilcoxon p=0.04), but not PAS density ≥ median, presenting symptoms or RD (p>0.28).ConclusionTumors from uveal melanoma patients that report a visual field shadow are likely to display VM and greater PAS density, likely explaining the previously reported association between this symptom and poor prognosis.

Systemic Mastocytosis in a Goat

1995 ◽  
Vol 32 (6) ◽  
pp. 719-721 ◽  
Author(s):  
K. N. M. Khan ◽  
J. E. Sagartz ◽  
G. Koenig ◽  
K. Tanaka
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Transmission Electron Microscopy ◽  
Mast Cells ◽  
Systemic Mastocytosis ◽  
Periodic Acid ◽  
Hypochromic Anemia ◽  
Postmortem Examination ◽  
Periodic Acid Schiff ◽  
Transmission Electron

Systemic mastocytosis was diagnosed in a 4-year-old, female Nubian goat. Clinically, the animal was depressed and had severe macrocytic hypochromic anemia and leukopenia. Postmortem examination revealed neoplastic mast cells invading the heart, lung, liver, spleen, lymph nodes, and bone marrow. Eosinophils were frequently admixed with infiltrating mast cells in all organs. Using routine light microscopy, histochemistry, and transmission electron microscopy, metachromatic and periodic acid—Schiff–positive granules were identified within the cytoplasm of neoplastic mast cells. Erythrophagocytosis was observed in some neoplastic cells, although its contribution to the anemia was not clear. This report represents the first description of mast cell neoplasia in the goat.

scholarly journals Single-cell Transcriptomic Analysis of Eutopic Endometrium and Ectopic Lesions of Adenomyosis

2020 ◽  
Author(s):  
Zhiyong Liu ◽  
Zhonghua Sun ◽  
Hongyun Liu ◽  
Weipin Niu ◽  
Xin Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Motility ◽  
Single Cell ◽  
Periodic Acid ◽  
Expression Patterns ◽  
Vasculogenic Mimicry ◽  
Cell Subpopulation ◽  
Periodic Acid Schiff ◽  
Eutopic Endometrium ◽  
Invasion And Migration

Abstract Background: Adenomyosis (AM) is a common benign chronic gynaecological disorder; however, the precise pathogenesis of adenomyosis is still poorly understood. Single-cell RNA sequencing (scRNA-seq) can uncover rare subpopulations, explore genetic and functional heterogeneity, and reveal the uniqueness of each cell. It provides us a new approach to reveal biological issues from a more detailed and microscopic perspective. Here, we utilize this revolutionary technology to identify the changes of gene expression patterns between ectopic lesions and the eutopic endometrium at the single-cell level and explore a potential novel pathogenesis of AM.Methods: A control endometrium (sample with leiomyoma excluding endometrial disorders, n=1), eutopic endometrium and ectopic lesion (from a patient with adenomyosis, n=1) samples were analysed by scRNA-seq, and additional leiomyoma (n=3) and adenomyosis (n=3) samples were used to confirm colocalization and vasculogenic mimicry (VM) formation. Protein colocalization was visualized by immunofluorescence, and CD34-periodic acid-Schiff (PAS) double staining was used to assess the formation of VM.Results: The scRNA-seq results suggest that cancer-, cell motility- and inflammation- (CMI) associated terms, cell proliferation and angiogenesis play important roles in the progression of AM. Moreover, the colocalization of EPCAM and PECAM1 increased significantly in the ectopic endometrium group (P < 0.05), cell subpopulation with high copy number variation (CNV) levels possessing tumour-like features existed in the ectopic lesion sample, and VNN1- and EPCAM-positive cell subcluster displayed active cell motility in endometrial epithelial cells. Furthermore, the epithelial cells transformed to endothelial cells with the obvious accumulation of vasculogenic mimicry formations (positively stained with PAS but not CD34, P < 0.05) in ectopic lesions.Conclusions: In the present study, our results support the theory of adenomyosis derived from the invasion and migration of the endometrium. Moreover, cell subcluster with high CNV level and tumour-associated characteristics is identified. Furthermore, epithelial-endothelial transition (EET) and the formation of VM in tumours, the latter of which facilitates the blood supply and plays an important role in maintaining cell growth, were also confirmed to occur in AM. These results indicated that the inhibition of EET and VM formation may be a potential strategy for AM management.

Effects of O3 on submucosal gland of bonnet monkey trachea

1983 ◽  
Vol 41 ◽  
pp. 678-679
Author(s):  
S. Yamashiro ◽  
D. Wilson ◽  
J. St. George ◽  
D. Hyde ◽  
C. Plopper ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Lung Parenchyma ◽  
Uranyl Acetate ◽  
Upper Airways ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Bonnet Monkey ◽  
Submucosal Glands

In the past, ozone inhalation studies have focused on the lower airways and lung parenchyma. The purpose of this study was to evaluate the effects of ozone on submucosal glands of upper airways. Six adult male bonnet monkeys were exposed to 0.64 ppm ozone continuously for 7 days, and three were exposed to chamber conditions without ozone. The animals were exsanguinated under barbiturate anesthesia. The trachea and lung were fixed by airway infusion of Karnovsky's fixative, which was adjusted to pH 7.4 and 440 milliOsmols. Sagittal sections of ventral trachea were embedded in glycol methacrylate and Araldite 502 for light and electron microscopy. One micrometer methacrylate sections were stained with Alcian blue-periodic acid Schiff (AB/PAS). Selected areas of Araldite-embedded tissue were sectioned for transmission electron microscopy, stained with uranyl acetate and lead citrate and examined with a Zeiss EM 10. Volume percentages of the lumen, granular and nongranular regions of fhe gland and the duct wall, respectively, were estimated by stereologic methods on AB/PAS stained sections.

scholarly journals Vasculogenic mimicry correlates to presenting symptoms and mortality in uveal melanoma

2021 ◽  
Author(s):  
Shiva Sabazade ◽  
Viktor Gill ◽  
Christina Herrspiegel ◽  
Gustav Stålhammar
Keyword(s):  
Visual Field ◽  
Uveal Melanoma ◽  
Poor Prognosis ◽  
Periodic Acid ◽  
Vasculogenic Mimicry ◽  
Clinicopathological Parameters ◽  
Tumor Diameter ◽  
Blurred Vision ◽  
Periodic Acid Schiff ◽  
Presenting Symptoms

Abstract Purpose Fluid-conducting extracellular matrix patterns known as vasculogenic mimicry (VM) have been associated with poor prognosis in uveal melanoma and other cancers. We investigate the correlations between VM, presenting symptoms, mortality, and the area density of periodic acid-Schiff positive histological patterns (PAS density). Methods Sixty-nine patients that underwent enucleation for uveal melanoma between 2000 and 2007 were included. Clinicopathological parameters presenting symptoms and outcomes were collected. Histological tumor sections were evaluated for VM and PAS density was quantified with digital image analysis. Results Thirty-four patients (49%) presented with blurred vision. 18 (26%) with a shadow in the visual field, 7 (10%) with photopsia and/or floaters, and 2 (3%) with metamorphopsia. Nine patients (13%) had no symptoms at all. Median follow-up was 16.7 years (SD 2.6). A shadow in the visual field, but no other symptom, was positively correlated with the presence of VM (φ 0.70, p < 0.001) and greater PAS density (p < 0.001). In multivariate regression, retinal detachment (RD), presence of VM, and PAS density ≥ median were independent predictors of a shadow, but not tumor distance to the macula, tumor apical thickness, tumor diameter, or ciliary body engagement. The presence of VM was associated with significantly shorter cumulative disease-specific survival (Wilcoxon p = 0.04), but not PAS density ≥ median, presenting symptoms or RD (p > 0.28). Conclusion Tumors from uveal melanoma patients that report a visual field shadow are likely to display VM and greater PAS density, likely explaining the previously reported association between this symptom and poor prognosis.

scholarly journals Avian pulmonary proteinosis: six cases and a review of the literature

2019 ◽  
Vol 31 (3) ◽  
pp. 378-381 ◽  
Author(s):  
Dayna A. Goldsmith ◽  
Aslı Mete ◽  
Joseph B. Pesavento ◽  
John M. Adaska
Keyword(s):  
Pulmonary Surfactant ◽  
Pulmonary Alveolar Proteinosis ◽  
Incidental Finding ◽  
Periodic Acid ◽  
Review Of The Literature ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Alveolar Proteinosis ◽  
Lamellar Material ◽  
Electron Lucent

Pulmonary alveolar proteinosis (PAP) is a disease of surfactant clearance in which functional abnormalities in alveolar macrophages lead to accumulation of surfactant within alveoli in mammals. Histologic examination of 6 avian autopsies, including 4 chickens, a turkey, and a cockatiel, revealed accumulation of hypereosinophilic densely arrayed lamellar material in the lungs that was magenta by periodic acid–Schiff stain and diastase resistant. Transmission electron microscopy of the proteinaceous material in 2 cases demonstrated alternating electron-dense and electron-lucent lamellae that formed whorls and had a regular periodicity of 6–14 nm, consistent with pulmonary surfactant. Given the anatomic differences between avian and mammalian lungs, we designated the presented condition “pulmonary proteinosis,” which can be observed as both an incidental finding or, when severe, may be a contributing factor to death through respiratory failure.

Hepatotoxic effects of melamine exposure from the weaning period in rats: a flow cytometric, electron microscopic, and histopathologic study

2021 ◽  
Author(s):  
Zuleyha Erisgin ◽  
Hasan Serdar Mutlu ◽  
Yavuz Tekelioglu ◽  
Engin Deveci ◽  
Ugur Seker
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Periodic Acid ◽  
Flow Cytometric Analysis ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Periodic Acid Schiff ◽  
Flow Cytometric ◽  
Transmission Electron

Abstract This study aims to investigate the effects of melamine exposure from the weaning period (21st postnatal days in rats) on liver tissue. Female Wistar albino rats (n = 18) were divided into three groups. About 0.1-ml saline was applied to the control group by gavage for 21 days from the postnatal 21st day. The second group was taken 50-mg/kg melamine (in 0.1-ml saline) and the third group was taken 75-mg/kg melamine (in 0.1-ml saline) p.o. On the postnatal 45th day, all rats were sacrificed under anesthesia. Then, liver tissues were cut into three parts and two of them placed in neutral formalin for histopathological and flow cytometric analysis, and one of them placed in 2.5% glutaraldehyde. Histopathological analysis was performed with hematoxylin & eosin, Masson trichrome, periodic acid Schiff stained sections, and also with transmission electron microscopy. Apoptosis (Annexin V positivity) was analyzed by flow cytometry. According to histopathological analysis, hepatocyte damage, sinusoidal dilatation, and inflammatory cell infiltration significantly increased in both melamine groups compared with the control group. Apoptosis significantly increased in the 50 and 75-mg melamine groups compared with the control group. In the results of transmission electron microscopy analysis, there was abnormal chromatin distribution in the hepatocyte nuclei, loss in the cristae of the mitochondria, and organelle loss in large areas in the cytoplasm in both melamine exposure groups. As result, melamine exposure from the weaning period causes liver damage with increasing doses.

scholarly journals Does Vasculogenic Mimicry Exist in Astrocytoma?

2005 ◽  
Vol 53 (8) ◽  
pp. 997-1002 ◽  
Author(s):  
Wei-Ying Yue ◽  
Zhong-Ping Chen
Keyword(s):  
Tumor Cells ◽  
Periodic Acid ◽  
Vasculogenic Mimicry ◽  
World Health ◽  
Glial Fibrillary Acid Protein ◽  
Melanoma Tumor ◽  
Periodic Acid Schiff ◽  
Weak Reaction ◽  
Positive Pattern ◽  
Endothelial Marker

Vasculogenic mimicry (VM) has been observed in melanoma and in some non-melanoma tumor types. It is unknown whether a similar VM phenomenon exists in astrocytoma. The present study was to examine 45 astrocytomas (including World Health Organization grade II 15 cases, grade III 15 cases, and grade IV 15 cases) by CD34 endothelial marker periodic acid-Schiff (PAS) dual staining to see if VM existing in these tumors. The results demonstrated that endothelium-lined vessels dominated the tumor microvasculature and stained positively for PAS, laminin, and endothelial marker. PAS-positive pattern of VM was found in two grade IV astrocytomas. Channels stained positively for PAS, laminin, and negatively for CD34 of the VM entrapped in the tumor tissue. Erythrocytes could be observed in some of these channels. In these networks of PAS-positive pattern, spots of weak reaction for CD34 were observed, suggesting the incorporation of VM channel and normal vessel. Furthermore, in astrocytoma, especially glioblastoma, focus of anaplastic tumor cells appeared with CD34 expression, whereas some tumor cells lost glial fibrillary acid protein expression. It is assumed that genetically deregulated tumor cells in astrocytoma could lose the astrocyte-specific protein and express inappropriate markers not expected in cells of astrocyte lineage. The present results suggest that VM phenomenon exists in some malignant astrocytoma.

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

2020 ◽  
Vol 26 (3) ◽  
pp. 551-566 ◽  
Author(s):  
Soha A. Soliman ◽  
Basma Mohamed Kamal ◽  
Alaa S. Abuo-Elhmad ◽  
Hanan H. Abd-Elhafeez
Keyword(s):  
Electron Microscopy ◽  
Periodic Acid ◽  
Detailed Structure ◽  
Periodic Acid Schiff ◽  
Transmission Electron ◽  
Different Types ◽  
Pas Staining ◽  
Precursor Layer ◽  
Histochemical Characterization

AbstractStudying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblast precursors differentiate and secrete enamel and dentine, respectively. We used different histochemical techniques, including Crossmon's trichrome staining, Weigert–Van Gieson staining, periodic acid–Schiff (PAS) staining, combined Alcian blue (AB; pH 2.5)/PAS staining, Weigert–Van Gieson staining, Mallory trichrome staining, and AB staining to distinguish the dentine and denticle ligaments. We used acridine orange to detect lysosome activity during denticle eruption. Transmission electron microscopy was used to detect the denticle ultrastructure, and scanning electron microscopy was used to detect the topographic distributions of different types of dermal tissues in different anatomical regions.

Malakoplakia in the Urinary Bladder of 4 Puppies

2021 ◽  
pp. 030098582110097
Author(s):  
Katelin L. Davis ◽  
Liang Cheng ◽  
José Ramos-Vara ◽  
Melissa D. Sánchez ◽  
Rebecca P. Wilkes ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Periodic Acid ◽  
Granular Cell ◽  
Periodic Acid Schiff ◽  
E Coli ◽  
Transmission Electron ◽  
Microscopic Features ◽  
Escherichia Coli Infection ◽  
Large Round ◽  
Hematoxylin And Eosin Stain

Malakoplakia in humans most often affects the urinary bladder and is characterized by inflammation with von Hansemann–type macrophages, with or without Michaelis-Gutmann bodies, and is frequently associated with Escherichia coli infection. We describe the microscopic features of malakoplakia in the urinary bladder of 4 puppies. In all cases, the lamina propria of the urinary bladder was markedly expanded by sheets of large, round to polygonal macrophages with intracytoplasmic, periodic acid-Schiff-positive granules and granular inclusions, and rare Prussian blue–positive inclusions. Macrophages were positive for CD18 and Iba1. In 2 cases, Michaelis-Gutmann bodies were detected with hematoxylin and eosin stain and were best demonstrated with von Kossa stain. E. coli infection was confirmed in 2 cases with bacterial culture or polymerase chain reaction (PCR) and sequencing of the bacterial 16S ribosomal RNA gene. Transmission electron microscopy of one case demonstrated macrophages with abundant lysosomes, phagolysosomes, and rod-shaped bacteria. Microscopic features were similar to human cases of malakoplakia. In dogs, the light microscopic characteristics of malakoplakia closely resemble granular cell tumors and histiocytic ulcerative colitis.

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